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The map label for this gene is trmD [H]

Identifier: 238923881

GI number: 238923881

Start: 1389056

End: 1390672

Strand: Direct

Name: trmD [H]

Synonym: EUBREC_1509

Alternate gene names: 238923881

Gene position: 1389056-1390672 (Clockwise)

Preceding gene: 238923880

Following gene: 238923882

Centisome position: 40.27

GC content: 43.48

Gene sequence:

>1617_bases
ATGAGATTTCATGTGCTGACCTTATTCCCACAGATGATAGAACAGGGACTGTCAGAGAGTATCACAGGCAGAGCTTTAAA
ACAGAATATTATATCACTCAATACGGTAAATATCAGGGATTTTGCGCACAATAAGCACAATAAGGTTGATGATTACACAT
ATGGTGGCGGTGCGGGGATGCTTATGCAGGCAGAGCCGGTGTATCAGGCTGTGCGCAGTGTGGTGAGTCAGATCAATAAA
TGTAATCAGGTCCATTCAGGTGATAATTCAGAGAAAAATATCGCCGATGAAAATATATTATATGAAAATACATCATATAA
AAATACAGCAGAAGAAATAAAAAATCACAATGCGCGTATGATTTATGTTACTCCACAGGGCCGCGTGTTCAACCAGCAGA
TGGCAGCAGAATTTGCAAAGTGTGATGACCTCATTTTTCTATGTGGACACTATGAAGGTATTGACGAGCGCGTGCTGGAG
GAGACGGTAACAGATTATGTTTCTATCGGTGACTATGTTCTAACGGGCGGAGAACTGCCGTCGATGGTAATGATAGATGC
GATTTCACGTCTGGTTCCTGGGGTGCTGCACAATGATATTTCGGCAGAGACAGAGTCGTTTCATGGCAATCTGCTCGAAT
ACCCTCAGTATTCACGTCCTGTTGAGTGGCATGACAAAAAGGTGCCTGAGGTACTCATGTCAGGCAATCAGAAAAAGATA
GATGCATGGCGCCTTGAAAAGTCTATTGAGCGCACAAAGGAGCGCAGGCCGGATTTGTATGCCGGGTTTAAAAGACTTGA
TAAATGCAGAGAGTTTCTGATGAAAAATAAACTGCTTCATATTGATATGATAGAGCTCATAAACAGGGGCTGCGCCGAGA
TACTGTTTGAGGCAGATGGCGAATATCTGCTTCGGGACATGGTCTCAAAGGTATGCTTTCATACAAGACCGGATGAGGGC
GAGTCGAAGCTTATTGATTTGGTGCAGGAAAATGTCACAAAGTCTGTTGATAAATACAGCAGTCAGCGTATTTCAAAAAA
CGTTACAGATCAGATTGTAAATGGCATAGTGCTTCACCAGCAAAGATATGTTGAGCTCTTTACAGCAAATGGCTTTAATG
AGACTGTAGAGTGCAGGCAGGCTGTGTATACAAACAAAGAAAAGCTATCTGTCAGCGGACTGTACAGACCGGATGGAAAG
CCGATGCCAAATGGCTTAACTATAAGAAAGCTGGATGCAGCTGATATACAGGAGGCTGCGCCAATGTACCCGGGCTTTGA
TAATCCGGATTATATCGTAGACAGGATAGAGGCAGGTGCTGTCTACGGAGCTTTTTTGAGTGATAATACTGCTGATGGTA
CCATTAATACCCTCGCGGGAATTATCGGAATCCATGAGGAGGGCAGCATAGGCATGCTCTATGTAAAGCTGCAGTACAGG
CATCAAAAGCTTGCCACAGCACTTGAAACATATGCTTTCAACAGGGCACTGGAGAACGGATGGATACCATATGGACAGAT
AATCGTGGGCAATGAGGCATCTATGAAGTTGCAGGAGAGTATGGGATTGCATTTTTCAAAATCATCCGTATACTGGATGA
CTAAAAATAATGCATAA

Upstream 100 bases:

>100_bases
CTCAAACTGCAAAAACCGTAAAATGAGATATAAAATTGAACAAAATCATACATTTTAAATCGTAAAACTGTAAAACAGGA
TGATATAGGAGATATAGGAA

Downstream 100 bases:

>100_bases
GGTAGGCAAAAGATAGTAAAAAATACTTGCAAATCCCATGAATCTGTGGTAAAGTACCAATGTTGTGAATAAAAGAGATG
GTCCTCTGTTGTGAAAATAG

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MRFHVLTLFPQMIEQGLSESITGRALKQNIISLNTVNIRDFAHNKHNKVDDYTYGGGAGMLMQAEPVYQAVRSVVSQINK
CNQVHSGDNSEKNIADENILYENTSYKNTAEEIKNHNARMIYVTPQGRVFNQQMAAEFAKCDDLIFLCGHYEGIDERVLE
ETVTDYVSIGDYVLTGGELPSMVMIDAISRLVPGVLHNDISAETESFHGNLLEYPQYSRPVEWHDKKVPEVLMSGNQKKI
DAWRLEKSIERTKERRPDLYAGFKRLDKCREFLMKNKLLHIDMIELINRGCAEILFEADGEYLLRDMVSKVCFHTRPDEG
ESKLIDLVQENVTKSVDKYSSQRISKNVTDQIVNGIVLHQQRYVELFTANGFNETVECRQAVYTNKEKLSVSGLYRPDGK
PMPNGLTIRKLDAADIQEAAPMYPGFDNPDYIVDRIEAGAVYGAFLSDNTADGTINTLAGIIGIHEEGSIGMLYVKLQYR
HQKLATALETYAFNRALENGWIPYGQIIVGNEASMKLQESMGLHFSKSSVYWMTKNNA

Sequences:

>Translated_538_residues
MRFHVLTLFPQMIEQGLSESITGRALKQNIISLNTVNIRDFAHNKHNKVDDYTYGGGAGMLMQAEPVYQAVRSVVSQINK
CNQVHSGDNSEKNIADENILYENTSYKNTAEEIKNHNARMIYVTPQGRVFNQQMAAEFAKCDDLIFLCGHYEGIDERVLE
ETVTDYVSIGDYVLTGGELPSMVMIDAISRLVPGVLHNDISAETESFHGNLLEYPQYSRPVEWHDKKVPEVLMSGNQKKI
DAWRLEKSIERTKERRPDLYAGFKRLDKCREFLMKNKLLHIDMIELINRGCAEILFEADGEYLLRDMVSKVCFHTRPDEG
ESKLIDLVQENVTKSVDKYSSQRISKNVTDQIVNGIVLHQQRYVELFTANGFNETVECRQAVYTNKEKLSVSGLYRPDGK
PMPNGLTIRKLDAADIQEAAPMYPGFDNPDYIVDRIEAGAVYGAFLSDNTADGTINTLAGIIGIHEEGSIGMLYVKLQYR
HQKLATALETYAFNRALENGWIPYGQIIVGNEASMKLQESMGLHFSKSSVYWMTKNNA
>Mature_538_residues
MRFHVLTLFPQMIEQGLSESITGRALKQNIISLNTVNIRDFAHNKHNKVDDYTYGGGAGMLMQAEPVYQAVRSVVSQINK
CNQVHSGDNSEKNIADENILYENTSYKNTAEEIKNHNARMIYVTPQGRVFNQQMAAEFAKCDDLIFLCGHYEGIDERVLE
ETVTDYVSIGDYVLTGGELPSMVMIDAISRLVPGVLHNDISAETESFHGNLLEYPQYSRPVEWHDKKVPEVLMSGNQKKI
DAWRLEKSIERTKERRPDLYAGFKRLDKCREFLMKNKLLHIDMIELINRGCAEILFEADGEYLLRDMVSKVCFHTRPDEG
ESKLIDLVQENVTKSVDKYSSQRISKNVTDQIVNGIVLHQQRYVELFTANGFNETVECRQAVYTNKEKLSVSGLYRPDGK
PMPNGLTIRKLDAADIQEAAPMYPGFDNPDYIVDRIEAGAVYGAFLSDNTADGTINTLAGIIGIHEEGSIGMLYVKLQYR
HQKLATALETYAFNRALENGWIPYGQIIVGNEASMKLQESMGLHFSKSSVYWMTKNNA

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=280, Percent_Identity=36.7857142857143, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 60932; Mature: 60932

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFHVLTLFPQMIEQGLSESITGRALKQNIISLNTVNIRDFAHNKHNKVDDYTYGGGAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCCCCCCCCE
LMQAEPVYQAVRSVVSQINKCNQVHSGDNSEKNIADENILYENTSYKNTAEEIKNHNARM
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCEE
IYVTPQGRVFNQQMAAEFAKCDDLIFLCGHYEGIDERVLEETVTDYVSIGDYVLTGGELP
EEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
SMVMIDAISRLVPGVLHNDISAETESFHGNLLEYPQYSRPVEWHDKKVPEVLMSGNQKKI
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHH
DAWRLEKSIERTKERRPDLYAGFKRLDKCREFLMKNKLLHIDMIELINRGCAEILFEADG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHCCC
EYLLRDMVSKVCFHTRPDEGESKLIDLVQENVTKSVDKYSSQRISKNVTDQIVNGIVLHQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRYVELFTANGFNETVECRQAVYTNKEKLSVSGLYRPDGKPMPNGLTIRKLDAADIQEAA
HHEEEEEECCCCCHHHHHHHHHHCCCHHEEECCEECCCCCCCCCCCEEEECCHHHHHHHC
PMYPGFDNPDYIVDRIEAGAVYGAFLSDNTADGTINTLAGIIGIHEEGSIGMLYVKLQYR
CCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHEECCCCCEEEEEEEEEHH
HQKLATALETYAFNRALENGWIPYGQIIVGNEASMKLQESMGLHFSKSSVYWMTKNNA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCEEECCEEEEEECCCC
>Mature Secondary Structure
MRFHVLTLFPQMIEQGLSESITGRALKQNIISLNTVNIRDFAHNKHNKVDDYTYGGGAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCCCCCCCCE
LMQAEPVYQAVRSVVSQINKCNQVHSGDNSEKNIADENILYENTSYKNTAEEIKNHNARM
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCEE
IYVTPQGRVFNQQMAAEFAKCDDLIFLCGHYEGIDERVLEETVTDYVSIGDYVLTGGELP
EEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
SMVMIDAISRLVPGVLHNDISAETESFHGNLLEYPQYSRPVEWHDKKVPEVLMSGNQKKI
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHH
DAWRLEKSIERTKERRPDLYAGFKRLDKCREFLMKNKLLHIDMIELINRGCAEILFEADG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHCCC
EYLLRDMVSKVCFHTRPDEGESKLIDLVQENVTKSVDKYSSQRISKNVTDQIVNGIVLHQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRYVELFTANGFNETVECRQAVYTNKEKLSVSGLYRPDGKPMPNGLTIRKLDAADIQEAA
HHEEEEEECCCCCHHHHHHHHHHCCCHHEEECCEECCCCCCCCCCCEEEECCHHHHHHHC
PMYPGFDNPDYIVDRIEAGAVYGAFLSDNTADGTINTLAGIIGIHEEGSIGMLYVKLQYR
CCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHEECCCCCEEEEEEEEEHH
HQKLATALETYAFNRALENGWIPYGQIIVGNEASMKLQESMGLHFSKSSVYWMTKNNA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCEEECCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA