Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 238923849

GI number: 238923849

Start: 1358256

End: 1359113

Strand: Direct

Name: dapF

Synonym: EUBREC_1477

Alternate gene names: 238923849

Gene position: 1358256-1359113 (Clockwise)

Preceding gene: 238923847

Following gene: 238923850

Centisome position: 39.37

GC content: 44.64

Gene sequence:

>858_bases
ATGAAATTTACGAAAATGCACGGCTGTGGAAATGATTATGTATATGTAAACCTTTTTGAAGAGAAGTTGGATGACCCGGC
AAGGGTGTCAATATATGTCAGTGACAGGCATTTTGGAATAGGCTCAGACGGACTAATCACAATAGGTCCGTCTGATAAGG
CAGATTTTAGAATGAGGATATATAATGCAGACGGTTCGGAAGCCGAGATGTGCGGAAACGGCATACGCTGCGTGGCGAAA
TACGTGTATGACCATAAACTTACTGACAAGACTGAAATATCGGTTGAGACAGGAGCAGGCATCAAGTACCTTACACTATA
TGTTGAGGAAAATAAGGTATCACAGGTGCGCGTGGATATGGGTGAGCCTATTCTGACGCCTGGGGACATTCCGGTTGTTA
AAGCAGACGGAAGTGCTTATAGTGATGACTACAGGGTAATAGATGAACCGATTTCAGCAGGCAATAGAGAGTGGCACATG
ACCTGTGTTTCAATGGGAAATCCTCATGCGGTAGTGTTTGTCGATGATGTGGCAGGTTTTGAGCTTGAGAAATACGGTCC
GCTTTTTGAGAATCATAAGATGTTTCCAAAGCGAACCAACACGGAGTTTGTAGAGATACTTTCAAGGAATGAGGCGAAAA
TGAGAGTATGGGAGAGGGGCAGCGCTGAGACATGGGCCTGCGGTACAGGAACCTGTGCGACCGTCATGGCATGTATACTC
AACAAAAAGACTGACAACAAGGTGCTTGTGCATCTAAGGGGCGGAGATCTGACTATCGAGTATATACCTGAAACCAATCA
TGTGTTTATGACTGGCCCTGCTACTGAGGTTTTCTCAGGAGAAATTGATATCATATAA

Upstream 100 bases:

>100_bases
CACAAAACGCAATATAAAACGCACAAAATGCTATAGAAAAACAAGAAAGTGCGCTGTATAATATAGTTACCAATATAAAA
CCAATAACGAGGTGATCACT

Downstream 100 bases:

>100_bases
AGCACAAGTATTGTGCGGTTTGGCGAATGTCTGTGGTTGAATAGTTACAAATCTTTAAGAAACAAAATTAACAGAAAATA
TATATCAAAAATATAATAAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKFTKMHGCGNDYVYVNLFEEKLDDPARVSIYVSDRHFGIGSDGLITIGPSDKADFRMRIYNADGSEAEMCGNGIRCVAK
YVYDHKLTDKTEISVETGAGIKYLTLYVEENKVSQVRVDMGEPILTPGDIPVVKADGSAYSDDYRVIDEPISAGNREWHM
TCVSMGNPHAVVFVDDVAGFELEKYGPLFENHKMFPKRTNTEFVEILSRNEAKMRVWERGSAETWACGTGTCATVMACIL
NKKTDNKVLVHLRGGDLTIEYIPETNHVFMTGPATEVFSGEIDII

Sequences:

>Translated_285_residues
MKFTKMHGCGNDYVYVNLFEEKLDDPARVSIYVSDRHFGIGSDGLITIGPSDKADFRMRIYNADGSEAEMCGNGIRCVAK
YVYDHKLTDKTEISVETGAGIKYLTLYVEENKVSQVRVDMGEPILTPGDIPVVKADGSAYSDDYRVIDEPISAGNREWHM
TCVSMGNPHAVVFVDDVAGFELEKYGPLFENHKMFPKRTNTEFVEILSRNEAKMRVWERGSAETWACGTGTCATVMACIL
NKKTDNKVLVHLRGGDLTIEYIPETNHVFMTGPATEVFSGEIDII
>Mature_285_residues
MKFTKMHGCGNDYVYVNLFEEKLDDPARVSIYVSDRHFGIGSDGLITIGPSDKADFRMRIYNADGSEAEMCGNGIRCVAK
YVYDHKLTDKTEISVETGAGIKYLTLYVEENKVSQVRVDMGEPILTPGDIPVVKADGSAYSDDYRVIDEPISAGNREWHM
TCVSMGNPHAVVFVDDVAGFELEKYGPLFENHKMFPKRTNTEFVEILSRNEAKMRVWERGSAETWACGTGTCATVMACIL
NKKTDNKVLVHLRGGDLTIEYIPETNHVFMTGPATEVFSGEIDII

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=286, Percent_Identity=38.8111888111888, Blast_Score=201, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_EUBR3 (C4Z900)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937365.1
- ProteinModelPortal:   C4Z900
- GeneID:   7963842
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1477
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31778; Mature: 31778

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 71-71 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKMHGCGNDYVYVNLFEEKLDDPARVSIYVSDRHFGIGSDGLITIGPSDKADFRMRI
CCEEEECCCCCCEEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEE
YNADGSEAEMCGNGIRCVAKYVYDHKLTDKTEISVETGAGIKYLTLYVEENKVSQVRVDM
ECCCCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCEEEEEEC
GEPILTPGDIPVVKADGSAYSDDYRVIDEPISAGNREWHMTCVSMGNPHAVVFVDDVAGF
CCCCCCCCCEEEEEECCCCCCCCCEEEECHHCCCCCEEEEEEEECCCCEEEEEEECCCCC
ELEKYGPLFENHKMFPKRTNTEFVEILSRNEAKMRVWERGSAETWACGTGTCATVMACIL
CHHHCCCCHHCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEECCCCHHHHHHHHHH
NKKTDNKVLVHLRGGDLTIEYIPETNHVFMTGPATEVFSGEIDII
CCCCCCEEEEEEECCCEEEEEECCCCEEEEECCCHHHCCCEEECC
>Mature Secondary Structure
MKFTKMHGCGNDYVYVNLFEEKLDDPARVSIYVSDRHFGIGSDGLITIGPSDKADFRMRI
CCEEEECCCCCCEEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEE
YNADGSEAEMCGNGIRCVAKYVYDHKLTDKTEISVETGAGIKYLTLYVEENKVSQVRVDM
ECCCCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCEEEEEEC
GEPILTPGDIPVVKADGSAYSDDYRVIDEPISAGNREWHMTCVSMGNPHAVVFVDDVAGF
CCCCCCCCCEEEEEECCCCCCCCCEEEECHHCCCCCEEEEEEEECCCCEEEEEEECCCCC
ELEKYGPLFENHKMFPKRTNTEFVEILSRNEAKMRVWERGSAETWACGTGTCATVMACIL
CHHHCCCCHHCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEECCCCHHHHHHHHHH
NKKTDNKVLVHLRGGDLTIEYIPETNHVFMTGPATEVFSGEIDII
CCCCCCEEEEEEECCCEEEEEECCCCEEEEECCCHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA