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The map label for this gene is murE [H]

Identifier: 238923850

GI number: 238923850

Start: 1359216

End: 1360703

Strand: Direct

Name: murE [H]

Synonym: EUBREC_1478

Alternate gene names: 238923850

Gene position: 1359216-1360703 (Clockwise)

Preceding gene: 238923849

Following gene: 238923851

Centisome position: 39.4

GC content: 43.08

Gene sequence:

>1488_bases
ATGGGAGAAAATAAAATGAAGCTTGAAAAATTACTTGAAAGAATGGATTATAAAGTATTAGCAGGAAGCACGGATATAGA
GATTTCCACACTCGTGTATGACTCGCGCAAGGTGGAGAAGGCATCGGTATTTGTGTGCATATCGGGAGCTGTGTCAGATG
CACATGATTTCATCCCTGATGTGGTAAAGAAAGGTGCAGCGGCAGTGGTTGTCGAAAAAGATGTGACTCCTATCGAAGGG
GTGACATATATTAAGGTAGATAATACAAGGCTTGCACTTGCGTGTATGTCAGCAGCTTATTTTGATTATCCGGCCGAGAA
GATAAAGACTATCGGTATTACAGGTACAAAGGGTAAGACCACTACCACATATATGGTAAAGTCAATTCTTGAGTCAGCCG
GCATAAAGACAGGACTTATCGGTACAATAGAGTCTATATGTGGTGACAAGAGAATTCCGTCAGCAAATACCACACCTGAG
TCATACAGAGTTCAGGAGCTGTTTAAGGAGATGGTGGATGAAGGCCTTGATGCAGTTGTTATGGAAGTGTCATCACAGGC
TCTAATGCTTCACAGAGTATCGGGCTTTACCTTTGATATCGGTGTATTTACAAATCTTGAGCCTGACCACATCGGAGAGC
ATGAGCACAAGGATTTTGCAGACTACATGCATTGCAAGAGTCTTTTATTCAGACAGTGCAGGCTTGGTATCTTTAACGGA
GATGATGAGCACTTAGAGGGTGTTATGAAGGGACACACATGCCAGGTGGAGACTTACGGCTACAAGTCCACAAACAACCT
GATAGCAGAAAATGTTGAGCTTAAAAAGGAGCATGGAGCACTCGGCGTTAAATATCATGTATCAGGGCTTCTGGATTTTG
ATGTGGAGGTCAATGTGCCGGGCAAATTCTCAGTATACAATTCACTAACAGCCATTGCTATCTGCCATCATTTCAATGTG
GATAAAAAGGCAATCGGGGAGGCTCTTCATCATGTGAGTGTAAAGGGCAGAATAGAGATTGTTCCTGTTACAAAGAGATA
TACCCTTATGATTGATTATGCACACAATGCGATGGCACTTGAAAGCCTTCTCACCACTCTTAAGGAGTATGAGCCGGGAA
GACTTGTATGCCTGTTTGGCTGTGGCGGAAACAGGGCAAAGTCACGCCGTTATGAGATGGGAGAGGTGTCATCGAGACTT
GCAGACCTTACTGTTGTCACATCCGATAATCCAAGAGATGAGGAGCCTATGGACATAATCAATGATATTTTACAGGGTGT
ACACAAGGCAGATGGAGAGTATGTGACAATTCCTGACCGTAAGGAGGCTATCAGATACTGCATGGAGAATGCCAAGGATG
GTGATATCATAGTACTTGCAGGAAAGGGCCATGAGGATTATCAGGAGATAAAGGGTGTGAAGCATCACATGGATGAGAGA
GAATTAATTGCTGATATAATAAAAGAAAATCCTGACATCAGACTTTAA

Upstream 100 bases:

>100_bases
CACAAGTATTGTGCGGTTTGGCGAATGTCTGTGGTTGAATAGTTACAAATCTTTAAGAAACAAAATTAACAGAAAATATA
TATCAAAAATATAATAAAAT

Downstream 100 bases:

>100_bases
TATTTTAGTATAGGTTAAATAGTTTAGTATAAACTAGTTCAGTAAAATAGTGTATTTGGGAGGTATATATGTCTCTGTTT
AAGGGCGCCGGAGTAGCGCT

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MGENKMKLEKLLERMDYKVLAGSTDIEISTLVYDSRKVEKASVFVCISGAVSDAHDFIPDVVKKGAAAVVVEKDVTPIEG
VTYIKVDNTRLALACMSAAYFDYPAEKIKTIGITGTKGKTTTTYMVKSILESAGIKTGLIGTIESICGDKRIPSANTTPE
SYRVQELFKEMVDEGLDAVVMEVSSQALMLHRVSGFTFDIGVFTNLEPDHIGEHEHKDFADYMHCKSLLFRQCRLGIFNG
DDEHLEGVMKGHTCQVETYGYKSTNNLIAENVELKKEHGALGVKYHVSGLLDFDVEVNVPGKFSVYNSLTAIAICHHFNV
DKKAIGEALHHVSVKGRIEIVPVTKRYTLMIDYAHNAMALESLLTTLKEYEPGRLVCLFGCGGNRAKSRRYEMGEVSSRL
ADLTVVTSDNPRDEEPMDIINDILQGVHKADGEYVTIPDRKEAIRYCMENAKDGDIIVLAGKGHEDYQEIKGVKHHMDER
ELIADIIKENPDIRL

Sequences:

>Translated_495_residues
MGENKMKLEKLLERMDYKVLAGSTDIEISTLVYDSRKVEKASVFVCISGAVSDAHDFIPDVVKKGAAAVVVEKDVTPIEG
VTYIKVDNTRLALACMSAAYFDYPAEKIKTIGITGTKGKTTTTYMVKSILESAGIKTGLIGTIESICGDKRIPSANTTPE
SYRVQELFKEMVDEGLDAVVMEVSSQALMLHRVSGFTFDIGVFTNLEPDHIGEHEHKDFADYMHCKSLLFRQCRLGIFNG
DDEHLEGVMKGHTCQVETYGYKSTNNLIAENVELKKEHGALGVKYHVSGLLDFDVEVNVPGKFSVYNSLTAIAICHHFNV
DKKAIGEALHHVSVKGRIEIVPVTKRYTLMIDYAHNAMALESLLTTLKEYEPGRLVCLFGCGGNRAKSRRYEMGEVSSRL
ADLTVVTSDNPRDEEPMDIINDILQGVHKADGEYVTIPDRKEAIRYCMENAKDGDIIVLAGKGHEDYQEIKGVKHHMDER
ELIADIIKENPDIRL
>Mature_494_residues
GENKMKLEKLLERMDYKVLAGSTDIEISTLVYDSRKVEKASVFVCISGAVSDAHDFIPDVVKKGAAAVVVEKDVTPIEGV
TYIKVDNTRLALACMSAAYFDYPAEKIKTIGITGTKGKTTTTYMVKSILESAGIKTGLIGTIESICGDKRIPSANTTPES
YRVQELFKEMVDEGLDAVVMEVSSQALMLHRVSGFTFDIGVFTNLEPDHIGEHEHKDFADYMHCKSLLFRQCRLGIFNGD
DEHLEGVMKGHTCQVETYGYKSTNNLIAENVELKKEHGALGVKYHVSGLLDFDVEVNVPGKFSVYNSLTAIAICHHFNVD
KKAIGEALHHVSVKGRIEIVPVTKRYTLMIDYAHNAMALESLLTTLKEYEPGRLVCLFGCGGNRAKSRRYEMGEVSSRLA
DLTVVTSDNPRDEEPMDIINDILQGVHKADGEYVTIPDRKEAIRYCMENAKDGDIIVLAGKGHEDYQEIKGVKHHMDERE
LIADIIKENPDIRL

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=482, Percent_Identity=32.5726141078838, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55019; Mature: 54887

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGENKMKLEKLLERMDYKVLAGSTDIEISTLVYDSRKVEKASVFVCISGAVSDAHDFIPD
CCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
VVKKGAAAVVVEKDVTPIEGVTYIKVDNTRLALACMSAAYFDYPAEKIKTIGITGTKGKT
HHHCCCEEEEEECCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCHHHEEEEEEECCCCCC
TTTYMVKSILESAGIKTGLIGTIESICGDKRIPSANTTPESYRVQELFKEMVDEGLDAVV
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
MEVSSQALMLHRVSGFTFDIGVFTNLEPDHIGEHEHKDFADYMHCKSLLFRQCRLGIFNG
HHHHHHHEEEEECCCCEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DDEHLEGVMKGHTCQVETYGYKSTNNLIAENVELKKEHGALGVKYHVSGLLDFDVEVNVP
CHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCEEHHCCCEEEEEEECEEEEEEEEECCC
GKFSVYNSLTAIAICHHFNVDKKAIGEALHHVSVKGRIEIVPVTKRYTLMIDYAHNAMAL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEECCEEEEEEECCEEEEEEEECCCHHHH
ESLLTTLKEYEPGRLVCLFGCGGNRAKSRRYEMGEVSSRLADLTVVTSDNPRDEEPMDII
HHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHEEEEEEECCCCCCCCHHHHH
NDILQGVHKADGEYVTIPDRKEAIRYCMENAKDGDIIVLAGKGHEDYQEIKGVKHHMDER
HHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCHH
ELIADIIKENPDIRL
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
GENKMKLEKLLERMDYKVLAGSTDIEISTLVYDSRKVEKASVFVCISGAVSDAHDFIPD
CCCHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
VVKKGAAAVVVEKDVTPIEGVTYIKVDNTRLALACMSAAYFDYPAEKIKTIGITGTKGKT
HHHCCCEEEEEECCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCHHHEEEEEEECCCCCC
TTTYMVKSILESAGIKTGLIGTIESICGDKRIPSANTTPESYRVQELFKEMVDEGLDAVV
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
MEVSSQALMLHRVSGFTFDIGVFTNLEPDHIGEHEHKDFADYMHCKSLLFRQCRLGIFNG
HHHHHHHEEEEECCCCEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DDEHLEGVMKGHTCQVETYGYKSTNNLIAENVELKKEHGALGVKYHVSGLLDFDVEVNVP
CHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCEEHHCCCEEEEEEECEEEEEEEEECCC
GKFSVYNSLTAIAICHHFNVDKKAIGEALHHVSVKGRIEIVPVTKRYTLMIDYAHNAMAL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEECCEEEEEEECCEEEEEEEECCCHHHH
ESLLTTLKEYEPGRLVCLFGCGGNRAKSRRYEMGEVSSRLADLTVVTSDNPRDEEPMDII
HHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHEEEEEEECCCCCCCCHHHHH
NDILQGVHKADGEYVTIPDRKEAIRYCMENAKDGDIIVLAGKGHEDYQEIKGVKHHMDER
HHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCHH
ELIADIIKENPDIRL
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]