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The map label for this gene is 238923781

Identifier: 238923781

GI number: 238923781

Start: 1283948

End: 1284481

Strand: Direct

Name: 238923781

Synonym: EUBREC_1403

Alternate gene names: NA

Gene position: 1283948-1284481 (Clockwise)

Preceding gene: 238923780

Following gene: 238923782

Centisome position: 37.22

GC content: 32.21

Gene sequence:

>534_bases
GTGTTCGGATATGCAAAATTAGAACTTGGAGAAGTATGTAGTTCGATTTCTTCAGGTAAATCAAAGGAAAGGTCAAAAAC
AGGACCATATCCTGTGTATGGTTCAACTGGAATTATCGCAAGAACCAATAGAGCTTCATATGACAAAACAAATATATTAA
TAGCTCGTGTTGGTGCTAACGCAGGTTATGTGCACCTTGCAAGTGGGAGTTATGATGTTTCAGATAACACCTTGATTGCG
GATATCAAACCGGAAAATAATTTAAAATATATATTTTATATATTGCAAAATATAGCATTGAACAGATTTGCTAAAGGTGG
TGGCCAGCCCTTAATAACAGCAGGAAAAATAAAGCAAATTGAAATTAAAATTCCAGATCAAATCACACAAGATAAAGTTG
TAAAAATTCTTGATGAATTTGAAATGATTTGTACAGATCTTAATGCTGGAATTCCTGCTGAGATTAATGTAAGGAATAAA
CAGTATGAATTTTATAGAGACAAATTAATGACATTTAAGGAGAAATTAAAGTGA

Upstream 100 bases:

>100_bases
AAAAACAATATGAATACTACAGGGATGCTTTATTGACATATGCTTCTACTGGAAAGATAATTGCTAGACAGACAGAACAT
AATTAGACTTGTTCAATAAT

Downstream 100 bases:

>100_bases
AAACCATAGCATTACGCTTTGGAGAACATTTTGCACCAGAATGCGGTACTATAGAAGCCCATAGAGCTGTTATAGAACAA
TATGGGTATGTCTGGTATGG

Product: anti-codon nuclease masking agent (PrrB)

Products: NA

Alternate protein names: S.MpnORFGP; Type I restriction enzyme specificity protein MPN_285; S protein [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MFGYAKLELGEVCSSISSGKSKERSKTGPYPVYGSTGIIARTNRASYDKTNILIARVGANAGYVHLASGSYDVSDNTLIA
DIKPENNLKYIFYILQNIALNRFAKGGGQPLITAGKIKQIEIKIPDQITQDKVVKILDEFEMICTDLNAGIPAEINVRNK
QYEFYRDKLMTFKEKLK

Sequences:

>Translated_177_residues
MFGYAKLELGEVCSSISSGKSKERSKTGPYPVYGSTGIIARTNRASYDKTNILIARVGANAGYVHLASGSYDVSDNTLIA
DIKPENNLKYIFYILQNIALNRFAKGGGQPLITAGKIKQIEIKIPDQITQDKVVKILDEFEMICTDLNAGIPAEINVRNK
QYEFYRDKLMTFKEKLK
>Mature_177_residues
MFGYAKLELGEVCSSISSGKSKERSKTGPYPVYGSTGIIARTNRASYDKTNILIARVGANAGYVHLASGSYDVSDNTLIA
DIKPENNLKYIFYILQNIALNRFAKGGGQPLITAGKIKQIEIKIPDQITQDKVVKILDEFEMICTDLNAGIPAEINVRNK
QYEFYRDKLMTFKEKLK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 19722; Mature: 19722

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGYAKLELGEVCSSISSGKSKERSKTGPYPVYGSTGIIARTNRASYDKTNILIARVGAN
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCC
AGYVHLASGSYDVSDNTLIADIKPENNLKYIFYILQNIALNRFAKGGGQPLITAGKIKQI
CCEEEEECCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEE
EIKIPDQITQDKVVKILDEFEMICTDLNAGIPAEINVRNKQYEFYRDKLMTFKEKLK
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGYAKLELGEVCSSISSGKSKERSKTGPYPVYGSTGIIARTNRASYDKTNILIARVGAN
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCC
AGYVHLASGSYDVSDNTLIADIKPENNLKYIFYILQNIALNRFAKGGGQPLITAGKIKQI
CCEEEEECCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEE
EIKIPDQITQDKVVKILDEFEMICTDLNAGIPAEINVRNKQYEFYRDKLMTFKEKLK
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]