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The map label for this gene is nadE [H]

Identifier: 238923736

GI number: 238923736

Start: 1229699

End: 1231606

Strand: Direct

Name: nadE [H]

Synonym: EUBREC_1358

Alternate gene names: 238923736

Gene position: 1229699-1231606 (Clockwise)

Preceding gene: 238923735

Following gene: 238923737

Centisome position: 35.65

GC content: 46.8

Gene sequence:

>1908_bases
TTGAAGGACGGATATATAAAGGTCGCTGCAGCGACACCAAAGGTAAAGGTAGCTGATACTGTATACAACGGACAGCTTAT
TAAAGCTCTTATGAAGGAGTGCACTGACAACGGGGCAAAGGTGGTTGTTTTCCCGGAGCTTTGCATTACCGGATACACCT
GTCAGGATCTTTTCTGGCAGGATAAGCTCATAGCTGCAGCAGAGGATGAGCTTATAGGGATAGCAGATTACAGCAGGAGC
CTTGACGGCATATTTTTCATAGGGCTTCCATATGAGATAAACGGCATGCTCTATAATATGGCGGCAGTTGTATCGCGCGG
AGAGGTGCTTGCCATGGTGCCAAAGACATTTCTGCCAAACTATAATGAGTTTTATGAGGCAAGGCATTTTGCATCGGGAG
AAAACTTAAGCACATATGTCACACTGAAAAATGGACAGCAGGTGTCTGTAGATACTGATTTTATTTTTAGCTGCAAGCAG
CTGCCAAAGCTTAAGATAGCGGTGGAGCTTTGTGAGGATCTTTGGACTCCGAACCCGCCGAGCATCAGACACGCGATGTC
TGGTGCCACTGTGATTGTAAATCTGTCTGCATCAGATGAGGTGACAGGCAAGGCAATATACAGGAGAGAGCTTGTGAGCG
GACAGTCTGCCAGATTAATCTGTGGATATATTTATGCGTCAGCGGGAGACGGTGAGTCCACACAGGATGTGGTCTACTCA
GGACACAATCTCATCTGTGAAAACGGAAATGTGCTTGCCGAGTCAGGGCGCTTTACAAATGAGACTATATACAGCGAGTT
TGATGTGGAAAGAATAGAGACTGAGAGGCGCAGGATGACAACCTTTGTTGTCGAGGATGACCACAGATGGGCTGAGTTTG
ACCTTGAGGTAAAGGATACCACTCTTAGCCGCTATGTGAATCCGGCTCCGTTTGTTCCGGCGGACAAGACAGACAGGGAC
AGACGCTGTGATGAGATTCTCATGATTCAGGCTATGGGACTTAAAAAGCGATTAGAGCATACCAACTGTAAGACAGCAGT
TATCGGTATTTCAGGAGGACTTGACTCCACATTGGCACTGCTTGTCACGGTAAGGGCATTTGATCTTTTGGGAAAGGATC
ACAAGGATATAGCGGCAGTTACTATGCCGGGCTTTGGAACAACAGACCGCACATATGACAATGCGGTAAACCTAATAAAA
TGCCTTGGTGCTACATTTATCGAGGTGGATATAAAGGATGCTGTAAATATACATTTCAGGGATATCGGACAGGATCCGTC
TGTGCATGATGTCACCTACGAGAATGGACAGGCACGCGAGCGCACACAGATACTTATGGATATCGCAAACAAGGAAAACG
GAATGGTCATAGGTACAGGAGATTTGTCAGAGCTGGCACTCGGCTGGGCTACCTACAACGGAGACCATATGTCCATGTAT
GCTGTAAATGCATCGGTTCCAAAGACTCTGGTGCGCCATCTGGTAAAATACTATGCAGATACCTGCGGCAGCGATTTGCT
GGAAAGTACACTTCTTGATGTGCTTGATACACCTGTTTCTCCTGAGCTTTTACCACCTGAAAACGGTAAGATTTCACAGA
AGACAGAGGATCTTGTGGGCCCTTATGAGCTGCATGACTTCTTCCTTTACAATATGCTGCGCTGCGGCTATGCTCCTGCC
AAGGTTTACCGCCTTGCAAGGATAGCATTTGAAGGAAAATATGATGATGAGTTTATTCTCAAATGGCTTAAGAACTTCTA
TCGCAGATTTTTCGCACAGCAGTTCAAGCGCTCATGTCTGCCGGATGGACCAAAGGTAGGAACAGTGGCGGTCTCACCGA
GAGGAGACCTAAGGATGCCTTCTGATGCGTGTGGACGTATCTGGATGGATGAGGTTGATAAGCTGTAA

Upstream 100 bases:

>100_bases
TGATTCCATTATCGCAGATGGCGAGAGCATTGAGGTAAAGTGCCAGTTCTGTAATAAGGCATATGAGTTTACGGTGGATG
AGTTGAAGGAGATGCGCTAA

Downstream 100 bases:

>100_bases
TAGTAAGAGGAGAACAATGGGAAAGACACATAAGAAAACTCATAGAAGGCAGGGAAGCATAAAACGTGCAAGGCACCGCG
ACAGGCGCATGAAAATAGGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MKDGYIKVAAATPKVKVADTVYNGQLIKALMKECTDNGAKVVVFPELCITGYTCQDLFWQDKLIAAAEDELIGIADYSRS
LDGIFFIGLPYEINGMLYNMAAVVSRGEVLAMVPKTFLPNYNEFYEARHFASGENLSTYVTLKNGQQVSVDTDFIFSCKQ
LPKLKIAVELCEDLWTPNPPSIRHAMSGATVIVNLSASDEVTGKAIYRRELVSGQSARLICGYIYASAGDGESTQDVVYS
GHNLICENGNVLAESGRFTNETIYSEFDVERIETERRRMTTFVVEDDHRWAEFDLEVKDTTLSRYVNPAPFVPADKTDRD
RRCDEILMIQAMGLKKRLEHTNCKTAVIGISGGLDSTLALLVTVRAFDLLGKDHKDIAAVTMPGFGTTDRTYDNAVNLIK
CLGATFIEVDIKDAVNIHFRDIGQDPSVHDVTYENGQARERTQILMDIANKENGMVIGTGDLSELALGWATYNGDHMSMY
AVNASVPKTLVRHLVKYYADTCGSDLLESTLLDVLDTPVSPELLPPENGKISQKTEDLVGPYELHDFFLYNMLRCGYAPA
KVYRLARIAFEGKYDDEFILKWLKNFYRRFFAQQFKRSCLPDGPKVGTVAVSPRGDLRMPSDACGRIWMDEVDKL

Sequences:

>Translated_635_residues
MKDGYIKVAAATPKVKVADTVYNGQLIKALMKECTDNGAKVVVFPELCITGYTCQDLFWQDKLIAAAEDELIGIADYSRS
LDGIFFIGLPYEINGMLYNMAAVVSRGEVLAMVPKTFLPNYNEFYEARHFASGENLSTYVTLKNGQQVSVDTDFIFSCKQ
LPKLKIAVELCEDLWTPNPPSIRHAMSGATVIVNLSASDEVTGKAIYRRELVSGQSARLICGYIYASAGDGESTQDVVYS
GHNLICENGNVLAESGRFTNETIYSEFDVERIETERRRMTTFVVEDDHRWAEFDLEVKDTTLSRYVNPAPFVPADKTDRD
RRCDEILMIQAMGLKKRLEHTNCKTAVIGISGGLDSTLALLVTVRAFDLLGKDHKDIAAVTMPGFGTTDRTYDNAVNLIK
CLGATFIEVDIKDAVNIHFRDIGQDPSVHDVTYENGQARERTQILMDIANKENGMVIGTGDLSELALGWATYNGDHMSMY
AVNASVPKTLVRHLVKYYADTCGSDLLESTLLDVLDTPVSPELLPPENGKISQKTEDLVGPYELHDFFLYNMLRCGYAPA
KVYRLARIAFEGKYDDEFILKWLKNFYRRFFAQQFKRSCLPDGPKVGTVAVSPRGDLRMPSDACGRIWMDEVDKL
>Mature_635_residues
MKDGYIKVAAATPKVKVADTVYNGQLIKALMKECTDNGAKVVVFPELCITGYTCQDLFWQDKLIAAAEDELIGIADYSRS
LDGIFFIGLPYEINGMLYNMAAVVSRGEVLAMVPKTFLPNYNEFYEARHFASGENLSTYVTLKNGQQVSVDTDFIFSCKQ
LPKLKIAVELCEDLWTPNPPSIRHAMSGATVIVNLSASDEVTGKAIYRRELVSGQSARLICGYIYASAGDGESTQDVVYS
GHNLICENGNVLAESGRFTNETIYSEFDVERIETERRRMTTFVVEDDHRWAEFDLEVKDTTLSRYVNPAPFVPADKTDRD
RRCDEILMIQAMGLKKRLEHTNCKTAVIGISGGLDSTLALLVTVRAFDLLGKDHKDIAAVTMPGFGTTDRTYDNAVNLIK
CLGATFIEVDIKDAVNIHFRDIGQDPSVHDVTYENGQARERTQILMDIANKENGMVIGTGDLSELALGWATYNGDHMSMY
AVNASVPKTLVRHLVKYYADTCGSDLLESTLLDVLDTPVSPELLPPENGKISQKTEDLVGPYELHDFFLYNMLRCGYAPA
KVYRLARIAFEGKYDDEFILKWLKNFYRRFFAQQFKRSCLPDGPKVGTVAVSPRGDLRMPSDACGRIWMDEVDKL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=607, Percent_Identity=23.2289950576606, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538756, Length=272, Percent_Identity=28.3088235294118, Blast_Score=102, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321865, Length=667, Percent_Identity=21.8890554722639, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641841, Length=605, Percent_Identity=21.6528925619835, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24641843, Length=605, Percent_Identity=21.6528925619835, Blast_Score=94, Evalue=4e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 70901; Mature: 70901

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDGYIKVAAATPKVKVADTVYNGQLIKALMKECTDNGAKVVVFPELCITGYTCQDLFWQ
CCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCHHHHHHC
DKLIAAAEDELIGIADYSRSLDGIFFIGLPYEINGMLYNMAAVVSRGEVLAMVPKTFLPN
CCEEEECCCCEEEEHHHCCCCCCEEEEECCEEECCHHHHHHHHHCCCCEEEECCHHHCCC
YNEFYEARHFASGENLSTYVTLKNGQQVSVDTDFIFSCKQLPKLKIAVELCEDLWTPNPP
HHHHHHHHHCCCCCCCEEEEEEECCCEEEECHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
SIRHAMSGATVIVNLSASDEVTGKAIYRRELVSGQSARLICGYIYASAGDGESTQDVVYS
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHEEC
GHNLICENGNVLAESGRFTNETIYSEFDVERIETERRRMTTFVVEDDHRWAEFDLEVKDT
CCEEEECCCCEEECCCCCCCCHHHHHCCHHHHHHHHHEEEEEEEECCCCEEEEEEEEECC
TLSRYVNPAPFVPADKTDRDRRCDEILMIQAMGLKKRLEHTNCKTAVIGISGGLDSTLAL
HHHHCCCCCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LVTVRAFDLLGKDHKDIAAVTMPGFGTTDRTYDNAVNLIKCLGATFIEVDIKDAVNIHFR
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEE
DIGQDPSVHDVTYENGQARERTQILMDIANKENGMVIGTGDLSELALGWATYNGDHMSMY
ECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHEEEEEECCCEEEEE
AVNASVPKTLVRHLVKYYADTCGSDLLESTLLDVLDTPVSPELLPPENGKISQKTEDLVG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
PYELHDFFLYNMLRCGYAPAKVYRLARIAFEGKYDDEFILKWLKNFYRRFFAQQFKRSCL
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDGPKVGTVAVSPRGDLRMPSDACGRIWMDEVDKL
CCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHCCC
>Mature Secondary Structure
MKDGYIKVAAATPKVKVADTVYNGQLIKALMKECTDNGAKVVVFPELCITGYTCQDLFWQ
CCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCHHHHHHC
DKLIAAAEDELIGIADYSRSLDGIFFIGLPYEINGMLYNMAAVVSRGEVLAMVPKTFLPN
CCEEEECCCCEEEEHHHCCCCCCEEEEECCEEECCHHHHHHHHHCCCCEEEECCHHHCCC
YNEFYEARHFASGENLSTYVTLKNGQQVSVDTDFIFSCKQLPKLKIAVELCEDLWTPNPP
HHHHHHHHHCCCCCCCEEEEEEECCCEEEECHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
SIRHAMSGATVIVNLSASDEVTGKAIYRRELVSGQSARLICGYIYASAGDGESTQDVVYS
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHEEC
GHNLICENGNVLAESGRFTNETIYSEFDVERIETERRRMTTFVVEDDHRWAEFDLEVKDT
CCEEEECCCCEEECCCCCCCCHHHHHCCHHHHHHHHHEEEEEEEECCCCEEEEEEEEECC
TLSRYVNPAPFVPADKTDRDRRCDEILMIQAMGLKKRLEHTNCKTAVIGISGGLDSTLAL
HHHHCCCCCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LVTVRAFDLLGKDHKDIAAVTMPGFGTTDRTYDNAVNLIKCLGATFIEVDIKDAVNIHFR
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEE
DIGQDPSVHDVTYENGQARERTQILMDIANKENGMVIGTGDLSELALGWATYNGDHMSMY
ECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHEEEEEECCCEEEEE
AVNASVPKTLVRHLVKYYADTCGSDLLESTLLDVLDTPVSPELLPPENGKISQKTEDLVG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
PYELHDFFLYNMLRCGYAPAKVYRLARIAFEGKYDDEFILKWLKNFYRRFFAQQFKRSCL
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDGPKVGTVAVSPRGDLRMPSDACGRIWMDEVDKL
CCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]