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The map label for this gene is nplT [H]

Identifier: 238923566

GI number: 238923566

Start: 1097947

End: 1099713

Strand: Direct

Name: nplT [H]

Synonym: EUBREC_1186

Alternate gene names: 238923566

Gene position: 1097947-1099713 (Clockwise)

Preceding gene: 238923565

Following gene: 238923567

Centisome position: 31.83

GC content: 42.44

Gene sequence:

>1767_bases
ATGGAAAAAGAAGCAATTTTGCATATTCCACTGTCACAATTTGCTTTTGCAGAAAAAGAAGACCGGCTGGTAATAAGAAT
CCGTTGTAAAAAGGGCGATTTAAAACGGTGCGTGCTGCTTTGGGGAGACCGGGTGGCAATGGAAGAGCCACTTCCGGTGA
AACAAAGTGAAATGGAACATGTGGCATCCGATATGCTTTATGATTATTATGAAGTTAAGATTACGTCTCCGTATACCAGG
GTATGCTATTATTTCCGGTTAGAGGACGATACAGAAAGCCTTTATTATTATGGAAGAGGATTTGCAGAGAAACTGTCCTG
TGGCAGGGCAGAATATTTCCAGTTTCCATATATTAGAAGAGAGGATATTTTAGATATCCCGGAGTGGGCAGAAGACATTG
TTATGTATCATATTTTCCCGGACAGTTTTGCAAATGACAGACGGAAACTGACGGAAAAAGCAACAAGGCATATGCTTTTG
CCGGATGGAAAAAGCGACAGCAATGGAAAAATTTTACCGGATCGGATTTGCAAGAATCATCTGGGTGGTACACTGAAAGG
AATTTGTGAAAACCTTGACTATATCGAAAAGCTTGGAATTAACTGCATTTATCTGAATCCGATTTTTGAAGCAGCGTCTT
ATCATAAATATGATACCATTGACTATTTTGAAATCGACCCCTGTCTTGGAAATAAAGCGGATTTAAAAGAACTGGTGCAG
CAGTGCCACAAGAGAGGAATCCGGGTTATTTTAGATGGAGTATTTAATCATTGTGGAGCAGATTTTTTTGCATTCCGGGA
TGTGCGGCAGAAGGGAAAAGCATCAAGATATTATAACTGGTTTTATCACCTGCCGGAAACGATACAGTACGCTGATCCGC
CAGATTATGAGGCGTTTGCCTATGTAAAGGAAATGCCAAAGCTTAATACCGGAAATCCTGAAGTGGTGGAGTACCTTTGC
AATGTTGGAACTTACTGGATTCGGGAAGCAGATATTGACGGCTGGCGCTTGGATGTGGCAAATGAAATCAATCATGAATT
CTGGAGAGCCTTCCGTCATGCGGTGAGAGCGGTAAAAGAGGATATATTTCTAATAGGCGAAATCTGGGAAGAAGCAGGCA
TCTGGCTTCAGGGAGACCAGTTTGATTCTACGATGAATTATACTTTTTCTTATCTGTGCCGGGATTTTTTTGGAAAGGGA
GAACTTTCGGTCAGTGAATTTGATGCCCAGATGCAGCGGATGATTTACCGCTACCCATGGCAGGTGAGCCTAGCACAGAT
GAATTTTCTGGATTCCCATGATATTCCGAGATTTTTAAGCTATTGCAATGGAAATAGAAAGAGAATGGAGCTGGCATTTT
TCTATCTCTTTATGGGAGTGGGCGTGCCATCGGTATTCTACGGGGATGAAGTGTATATCGATGGAATGAAAGAACTGGAG
TATCGGGCAGCCATGGACTGGCAGGCAGTAATGGAGCAGCAGGCAGAAAACAACAGATTACAGGGAAATCTGGCAGAGAA
GTTCAGCTGCTGGATTGCACTCCGAAAAGAGCATGTGGCATTACGCAAGGGCAATTACCGGACAATTTACTGCAATGATA
TGATGGGAGTATATGTATTTTTACGTAGTTTTGGGGACGACAAAGTCCTGGTATTTTTAAATACCGGCACCGGCAGTGTG
ACACTGACATCAGTGGAGGTTCCGCAGCTGCAGGAAAAAAAGATAATTCTTTCTTCTTGTGAGGGAAAGCTTTTGGTTGT
TTTGTAA

Upstream 100 bases:

>100_bases
GCTATCCTGGTATCTGTTCCGGTGCTGATTATTTTCTTCTGTATACAAAAGTATATGGTATCCGGTCTTACTGCAGGTGG
TGTGAAAGGATAGAAATAAA

Downstream 100 bases:

>100_bases
AACAAAAGATATTTCTATAAATAGTACCGTTCCTTAAAAAGATAAGTGACATCTTTACATACTTGTGCTATTTTTTGGAA
AAGATAAAGAGACATAGGAA

Product: neopullulanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MEKEAILHIPLSQFAFAEKEDRLVIRIRCKKGDLKRCVLLWGDRVAMEEPLPVKQSEMEHVASDMLYDYYEVKITSPYTR
VCYYFRLEDDTESLYYYGRGFAEKLSCGRAEYFQFPYIRREDILDIPEWAEDIVMYHIFPDSFANDRRKLTEKATRHMLL
PDGKSDSNGKILPDRICKNHLGGTLKGICENLDYIEKLGINCIYLNPIFEAASYHKYDTIDYFEIDPCLGNKADLKELVQ
QCHKRGIRVILDGVFNHCGADFFAFRDVRQKGKASRYYNWFYHLPETIQYADPPDYEAFAYVKEMPKLNTGNPEVVEYLC
NVGTYWIREADIDGWRLDVANEINHEFWRAFRHAVRAVKEDIFLIGEIWEEAGIWLQGDQFDSTMNYTFSYLCRDFFGKG
ELSVSEFDAQMQRMIYRYPWQVSLAQMNFLDSHDIPRFLSYCNGNRKRMELAFFYLFMGVGVPSVFYGDEVYIDGMKELE
YRAAMDWQAVMEQQAENNRLQGNLAEKFSCWIALRKEHVALRKGNYRTIYCNDMMGVYVFLRSFGDDKVLVFLNTGTGSV
TLTSVEVPQLQEKKIILSSCEGKLLVVL

Sequences:

>Translated_588_residues
MEKEAILHIPLSQFAFAEKEDRLVIRIRCKKGDLKRCVLLWGDRVAMEEPLPVKQSEMEHVASDMLYDYYEVKITSPYTR
VCYYFRLEDDTESLYYYGRGFAEKLSCGRAEYFQFPYIRREDILDIPEWAEDIVMYHIFPDSFANDRRKLTEKATRHMLL
PDGKSDSNGKILPDRICKNHLGGTLKGICENLDYIEKLGINCIYLNPIFEAASYHKYDTIDYFEIDPCLGNKADLKELVQ
QCHKRGIRVILDGVFNHCGADFFAFRDVRQKGKASRYYNWFYHLPETIQYADPPDYEAFAYVKEMPKLNTGNPEVVEYLC
NVGTYWIREADIDGWRLDVANEINHEFWRAFRHAVRAVKEDIFLIGEIWEEAGIWLQGDQFDSTMNYTFSYLCRDFFGKG
ELSVSEFDAQMQRMIYRYPWQVSLAQMNFLDSHDIPRFLSYCNGNRKRMELAFFYLFMGVGVPSVFYGDEVYIDGMKELE
YRAAMDWQAVMEQQAENNRLQGNLAEKFSCWIALRKEHVALRKGNYRTIYCNDMMGVYVFLRSFGDDKVLVFLNTGTGSV
TLTSVEVPQLQEKKIILSSCEGKLLVVL
>Mature_588_residues
MEKEAILHIPLSQFAFAEKEDRLVIRIRCKKGDLKRCVLLWGDRVAMEEPLPVKQSEMEHVASDMLYDYYEVKITSPYTR
VCYYFRLEDDTESLYYYGRGFAEKLSCGRAEYFQFPYIRREDILDIPEWAEDIVMYHIFPDSFANDRRKLTEKATRHMLL
PDGKSDSNGKILPDRICKNHLGGTLKGICENLDYIEKLGINCIYLNPIFEAASYHKYDTIDYFEIDPCLGNKADLKELVQ
QCHKRGIRVILDGVFNHCGADFFAFRDVRQKGKASRYYNWFYHLPETIQYADPPDYEAFAYVKEMPKLNTGNPEVVEYLC
NVGTYWIREADIDGWRLDVANEINHEFWRAFRHAVRAVKEDIFLIGEIWEEAGIWLQGDQFDSTMNYTFSYLCRDFFGKG
ELSVSEFDAQMQRMIYRYPWQVSLAQMNFLDSHDIPRFLSYCNGNRKRMELAFFYLFMGVGVPSVFYGDEVYIDGMKELE
YRAAMDWQAVMEQQAENNRLQGNLAEKFSCWIALRKEHVALRKGNYRTIYCNDMMGVYVFLRSFGDDKVLVFLNTGTGSV
TLTSVEVPQLQEKKIILSSCEGKLLVVL

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=233, Percent_Identity=25.3218884120172, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786604, Length=577, Percent_Identity=29.1161178509532, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1790687, Length=418, Percent_Identity=25.5980861244019, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789995, Length=526, Percent_Identity=23.7642585551331, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322245, Length=245, Percent_Identity=26.9387755102041, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=241, Percent_Identity=24.896265560166, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=241, Percent_Identity=24.896265560166, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=241, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=160, Percent_Identity=28.75, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=160, Percent_Identity=28.75, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321726, Length=241, Percent_Identity=24.0663900414938, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221330053, Length=218, Percent_Identity=27.9816513761468, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583749, Length=245, Percent_Identity=27.3469387755102, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583747, Length=245, Percent_Identity=27.3469387755102, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586587, Length=246, Percent_Identity=26.8292682926829, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586593, Length=217, Percent_Identity=27.1889400921659, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24586599, Length=179, Percent_Identity=27.9329608938547, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24586591, Length=218, Percent_Identity=25.6880733944954, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24586597, Length=234, Percent_Identity=25.2136752136752, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 68907; Mature: 68907

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEAILHIPLSQFAFAEKEDRLVIRIRCKKGDLKRCVLLWGDRVAMEEPLPVKQSEMEH
CCCCEEEECCHHHHHCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
VASDMLYDYYEVKITSPYTRVCYYFRLEDDTESLYYYGRGFAEKLSCGRAEYFQFPYIRR
HHHHHHHEEEEEEECCCCEEEEEEEEEECCCCEEEEECCCHHHHHCCCCHHHHCCCCCCC
EDILDIPEWAEDIVMYHIFPDSFANDRRKLTEKATRHMLLPDGKSDSNGKILPDRICKNH
CCCCCCCHHHHHEEEEEECCCCHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHHHHH
LGGTLKGICENLDYIEKLGINCIYLNPIFEAASYHKYDTIDYFEIDPCLGNKADLKELVQ
CCCHHHHHHHCHHHHHHCCCCEEEECHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
QCHKRGIRVILDGVFNHCGADFFAFRDVRQKGKASRYYNWFYHLPETIQYADPPDYEAFA
HHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEHHEEECCCCCCCCCCCCHHHHH
YVKEMPKLNTGNPEVVEYLCNVGTYWIREADIDGWRLDVANEINHEFWRAFRHAVRAVKE
HHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEHHHHCCHHHHHHHHHHHHHHHH
DIFLIGEIWEEAGIWLQGDQFDSTMNYTFSYLCRDFFGKGELSVSEFDAQMQRMIYRYPW
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
QVSLAQMNFLDSHDIPRFLSYCNGNRKRMELAFFYLFMGVGVPSVFYGDEVYIDGMKELE
EEEEHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCHHHHH
YRAAMDWQAVMEQQAENNRLQGNLAEKFSCWIALRKEHVALRKGNYRTIYCNDMMGVYVF
HHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHH
LRSFGDDKVLVFLNTGTGSVTLTSVEVPQLQEKKIILSSCEGKLLVVL
HHHCCCCEEEEEEECCCCEEEEEEEECCCCCCCHHHEECCCCCEEEEC
>Mature Secondary Structure
MEKEAILHIPLSQFAFAEKEDRLVIRIRCKKGDLKRCVLLWGDRVAMEEPLPVKQSEMEH
CCCCEEEECCHHHHHCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
VASDMLYDYYEVKITSPYTRVCYYFRLEDDTESLYYYGRGFAEKLSCGRAEYFQFPYIRR
HHHHHHHEEEEEEECCCCEEEEEEEEEECCCCEEEEECCCHHHHHCCCCHHHHCCCCCCC
EDILDIPEWAEDIVMYHIFPDSFANDRRKLTEKATRHMLLPDGKSDSNGKILPDRICKNH
CCCCCCCHHHHHEEEEEECCCCHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHHHHH
LGGTLKGICENLDYIEKLGINCIYLNPIFEAASYHKYDTIDYFEIDPCLGNKADLKELVQ
CCCHHHHHHHCHHHHHHCCCCEEEECHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
QCHKRGIRVILDGVFNHCGADFFAFRDVRQKGKASRYYNWFYHLPETIQYADPPDYEAFA
HHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEHHEEECCCCCCCCCCCCHHHHH
YVKEMPKLNTGNPEVVEYLCNVGTYWIREADIDGWRLDVANEINHEFWRAFRHAVRAVKE
HHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEHHHHCCHHHHHHHHHHHHHHHH
DIFLIGEIWEEAGIWLQGDQFDSTMNYTFSYLCRDFFGKGELSVSEFDAQMQRMIYRYPW
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
QVSLAQMNFLDSHDIPRFLSYCNGNRKRMELAFFYLFMGVGVPSVFYGDEVYIDGMKELE
EEEEHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCHHHHH
YRAAMDWQAVMEQQAENNRLQGNLAEKFSCWIALRKEHVALRKGNYRTIYCNDMMGVYVF
HHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHH
LRSFGDDKVLVFLNTGTGSVTLTSVEVPQLQEKKIILSSCEGKLLVVL
HHHCCCCEEEEEEECCCCEEEEEEEECCCCCCCHHHEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]