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The map label for this gene is gatB

Identifier: 238923550

GI number: 238923550

Start: 1078118

End: 1079545

Strand: Direct

Name: gatB

Synonym: EUBREC_1170

Alternate gene names: 238923550

Gene position: 1078118-1079545 (Clockwise)

Preceding gene: 238923549

Following gene: 238923551

Centisome position: 31.25

GC content: 46.43

Gene sequence:

>1428_bases
ATGAAAAACTATGAGACAGTCATCGGACTTGAGGTCCATGTAGAGTTAGCTACAAAAACAAAGATCTTTTGCGCCTGCTC
CACAGCATTCGGAGGCGCACCAAATACACATACATGTCCTGTCTGCACAGGACAGCCGGGCTCACTTCCTGTTTTAAACA
AGCAGGTTGTAGAGTACGCAGTGGCAGTAGGACTTGCCACCAACTGTACCATCACACAGTACAGCAAATTTGACAGAAAG
AACTATTTTTATCCGGATAACCCGCAGAACTATCAGATATCACAGCTTTATCTGCCAATCTGCCGCAACGGTTCAGTGGA
GATTGAGACAGCAAACGGAAAGAAAAACGTCAGAATCCATGAAATCCACATGGAGGAGGATGCCGGAAAGCTCGTACATG
ACGAGTGGGAGGACGTATCTATCGTAGATTACAACCGTTCCGGAGTACCTCTTATAGAGATAGTATCAGAGCCTGATATG
CGCTCCGCTGAGGAGGTTATCGCATACCTTGAGACTCTGCGTCAGACCATCCAGTATCTCGGAGCATCTGACTGTAAATT
GAATGAGGGCTCAATGCGTGCCGATGTCAACATCTCAGTCAGAGAGGTGGGCGCAAAGAAGTTTGGTACACGTACGGAGA
TGAAAAACCTTAACTCCTTCAAGGCTATCGCACATGCCATCGAGGGTGAGCGTGAGCGTCAGATAGAGCTTCTTGAGATG
GGCAGAAAGGTAGTACAGGAGACACGTAGATGGGATGACAACAAGGAATCATCACATGCCATGCGTTCAAAAGAGGATGC
GCAGGACTACAGATATTTCCCTGAGCCTGACCTCGTGCCAATCGTCGTATCTGATGAATGGATTGCACAGATAAAGGCAA
AGCAACCTGAGCTTCGTCCACAGAAGCTTGCACGCTACAAGAAGGAATACGACATCCCTGAGTATGATGCAAAGATTCTC
ACAGAGTCAAAGCATATGGCAGACATATTTGAGGCTGCCACAAAGCTCTGCGGCAAGCCGAAGAAGGTTTCAAACTGGCT
GATGGTTGAGACAATGCGATTGTTAAAGGATAATGATATGGATGCAGATGCGATAAAATTCTCACCTGTAAACCTTGCAA
AGCTTGTAGATCTGGTGGATGCAGGCACGATCAACTCATCTGTTGCCAAGGAAGTATTTGAGAAAATATTCCGGGAGGAT
ATTGATCCTGAGCAGTATGTCGAGGAGAACGGTCTTAAGAGCATGAATGATGAGGGTGAGCTTAAAGTTACAATCCAGGC
TGTCATCGATGCAAATCCACAGGCAGTTGAGGATTATCACAATGGCAAGAAGAAAGCCATCGGAGCACTTGTAGGTCAGA
CCATGAAGGCCACAAAGGGCAAGGCAAATCCTGCTGTTGTCAATAAGCTTCTTATGGAGTTGTTATAA

Upstream 100 bases:

>100_bases
AGAGCTTAAGCGCAGCACTATTGCGGAGGAATACAGTAACAAAAATACAGCAGATACCAATAAATCTGCCGGTGCACAGT
AGGAAGGAGGCAAAAAGGAT

Downstream 100 bases:

>100_bases
TTGGATAGATATTAAGAAAATGGATCCGGCAGGTCTTTTGATCTGTCGGTTCTTTTTTTTCAAAAGCGCCTCAGCTTGCT
TGCACGCATTTATTCAGTAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKNYETVIGLEVHVELATKTKIFCACSTAFGGAPNTHTCPVCTGQPGSLPVLNKQVVEYAVAVGLATNCTITQYSKFDRK
NYFYPDNPQNYQISQLYLPICRNGSVEIETANGKKNVRIHEIHMEEDAGKLVHDEWEDVSIVDYNRSGVPLIEIVSEPDM
RSAEEVIAYLETLRQTIQYLGASDCKLNEGSMRADVNISVREVGAKKFGTRTEMKNLNSFKAIAHAIEGERERQIELLEM
GRKVVQETRRWDDNKESSHAMRSKEDAQDYRYFPEPDLVPIVVSDEWIAQIKAKQPELRPQKLARYKKEYDIPEYDAKIL
TESKHMADIFEAATKLCGKPKKVSNWLMVETMRLLKDNDMDADAIKFSPVNLAKLVDLVDAGTINSSVAKEVFEKIFRED
IDPEQYVEENGLKSMNDEGELKVTIQAVIDANPQAVEDYHNGKKKAIGALVGQTMKATKGKANPAVVNKLLMELL

Sequences:

>Translated_475_residues
MKNYETVIGLEVHVELATKTKIFCACSTAFGGAPNTHTCPVCTGQPGSLPVLNKQVVEYAVAVGLATNCTITQYSKFDRK
NYFYPDNPQNYQISQLYLPICRNGSVEIETANGKKNVRIHEIHMEEDAGKLVHDEWEDVSIVDYNRSGVPLIEIVSEPDM
RSAEEVIAYLETLRQTIQYLGASDCKLNEGSMRADVNISVREVGAKKFGTRTEMKNLNSFKAIAHAIEGERERQIELLEM
GRKVVQETRRWDDNKESSHAMRSKEDAQDYRYFPEPDLVPIVVSDEWIAQIKAKQPELRPQKLARYKKEYDIPEYDAKIL
TESKHMADIFEAATKLCGKPKKVSNWLMVETMRLLKDNDMDADAIKFSPVNLAKLVDLVDAGTINSSVAKEVFEKIFRED
IDPEQYVEENGLKSMNDEGELKVTIQAVIDANPQAVEDYHNGKKKAIGALVGQTMKATKGKANPAVVNKLLMELL
>Mature_475_residues
MKNYETVIGLEVHVELATKTKIFCACSTAFGGAPNTHTCPVCTGQPGSLPVLNKQVVEYAVAVGLATNCTITQYSKFDRK
NYFYPDNPQNYQISQLYLPICRNGSVEIETANGKKNVRIHEIHMEEDAGKLVHDEWEDVSIVDYNRSGVPLIEIVSEPDM
RSAEEVIAYLETLRQTIQYLGASDCKLNEGSMRADVNISVREVGAKKFGTRTEMKNLNSFKAIAHAIEGERERQIELLEM
GRKVVQETRRWDDNKESSHAMRSKEDAQDYRYFPEPDLVPIVVSDEWIAQIKAKQPELRPQKLARYKKEYDIPEYDAKIL
TESKHMADIFEAATKLCGKPKKVSNWLMVETMRLLKDNDMDADAIKFSPVNLAKLVDLVDAGTINSSVAKEVFEKIFRED
IDPEQYVEENGLKSMNDEGELKVTIQAVIDANPQAVEDYHNGKKKAIGALVGQTMKATKGKANPAVVNKLLMELL

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=490, Percent_Identity=37.9591836734694, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI115534094, Length=301, Percent_Identity=36.2126245847176, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=31.1538461538462, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45550808, Length=492, Percent_Identity=31.5040650406504, Blast_Score=261, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_EUBR3 (C4ZHC0)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937066.1
- GeneID:   7964183
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1170
- ProtClustDB:   CLSK2506101
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53501; Mature: 53501

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYETVIGLEVHVELATKTKIFCACSTAFGGAPNTHTCPVCTGQPGSLPVLNKQVVEYA
CCCCCEEEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHH
VAVGLATNCTITQYSKFDRKNYFYPDNPQNYQISQLYLPICRNGSVEIETANGKKNVRIH
HHHHCCCCCEEHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCEEEE
EIHMEEDAGKLVHDEWEDVSIVDYNRSGVPLIEIVSEPDMRSAEEVIAYLETLRQTIQYL
EEEECCCCCCHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
GASDCKLNEGSMRADVNISVREVGAKKFGTRTEMKNLNSFKAIAHAIEGERERQIELLEM
CCCCCEECCCCEEEEEEEEHHHHCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GRKVVQETRRWDDNKESSHAMRSKEDAQDYRYFPEPDLVPIVVSDEWIAQIKAKQPELRP
HHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCH
QKLARYKKEYDIPEYDAKILTESKHMADIFEAATKLCGKPKKVSNWLMVETMRLLKDNDM
HHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
DADAIKFSPVNLAKLVDLVDAGTINSSVAKEVFEKIFREDIDPEQYVEENGLKSMNDEGE
CCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC
LKVTIQAVIDANPQAVEDYHNGKKKAIGALVGQTMKATKGKANPAVVNKLLMELL
EEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKNYETVIGLEVHVELATKTKIFCACSTAFGGAPNTHTCPVCTGQPGSLPVLNKQVVEYA
CCCCCEEEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHH
VAVGLATNCTITQYSKFDRKNYFYPDNPQNYQISQLYLPICRNGSVEIETANGKKNVRIH
HHHHCCCCCEEHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCEEEE
EIHMEEDAGKLVHDEWEDVSIVDYNRSGVPLIEIVSEPDMRSAEEVIAYLETLRQTIQYL
EEEECCCCCCHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
GASDCKLNEGSMRADVNISVREVGAKKFGTRTEMKNLNSFKAIAHAIEGERERQIELLEM
CCCCCEECCCCEEEEEEEEHHHHCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GRKVVQETRRWDDNKESSHAMRSKEDAQDYRYFPEPDLVPIVVSDEWIAQIKAKQPELRP
HHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCH
QKLARYKKEYDIPEYDAKILTESKHMADIFEAATKLCGKPKKVSNWLMVETMRLLKDNDM
HHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
DADAIKFSPVNLAKLVDLVDAGTINSSVAKEVFEKIFREDIDPEQYVEENGLKSMNDEGE
CCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC
LKVTIQAVIDANPQAVEDYHNGKKKAIGALVGQTMKATKGKANPAVVNKLLMELL
EEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA