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The map label for this gene is gatA

Identifier: 238923549

GI number: 238923549

Start: 1076588

End: 1078099

Strand: Direct

Name: gatA

Synonym: EUBREC_1169

Alternate gene names: 238923549

Gene position: 1076588-1078099 (Clockwise)

Preceding gene: 238923548

Following gene: 238923550

Centisome position: 31.21

GC content: 45.83

Gene sequence:

>1512_bases
ATGGATTTAATGTCTTTAACTGCCGTGGAACTCGGCAAGAAAATACAGGCAAAGGAAGTTACAGTGGAAGAGGCTGTAAA
GGCTGCCATTGCTTCTATAAAAGCAAAAGAAGAAAAGATAAACAGCTTTGTCACAATAGACGAAGAGGGTGCACTTAAAA
AAGCAGCAGAGGTTCAGGCAAAAATTGATGCGGGAGAGCTAAAAGGAGCGCTTGCGGGTGTGCCTGTAGCCATAAAGGAT
AACATGTGTACAGAAGGGCTTCTCACCACATGTTCATCAAAGATACTCTACAATTTCATCCCGACCTACACAGCGGAGGC
TGTTAAGCGCCTTGAGGATGCAGGCTGTGTCATCGTTGGAAAAACCAACATGGATGAGTTTGCCATGGGAAGCACCACAG
AGACATCTGCATTTGGAGCAACCAAAAACCCATGGAATACAGAGCACGTACCGGGAGGTTCATCAGGTGGAAGCTGTGCA
GCAGTTGCAGCAGAGGAGGTACCGTTTGCACTCGGTTCAGATACAGGCGGATCAATCAGACAGCCAAGCTCCTTTTGTGG
TGTGACAGGAATCAAGCCAACCTACGGCACAGTTTCACGATATGGACTCATTGCCTATGGCTCATCGCTTGACCAGATTG
GACCTATCGCAAAGGATGTCACAGACTGTGCTATTATACTTGAGGCAATCGCTTCATATGATACGAAGGATTCGACATCA
GTAAACAGAGACGATTTGAAGTTTACAGAGGCGCTTGTCGATGACGTAAAGGGTATGAAGATTGGTATTCCAAAGGATTA
TCTCGGAGATGGACTTGACCCGGAGGTAAAATCGGCAATCCTTGCAGCGGCAGATGAATTAAAGAAAAAAGGTGCTGTTG
TGGAGGAATTTGACCTCGGACTTGTAGAGTATGCCATTCCTGCGTACTATGTAATCGCCTGTGCTGAGGCAAGCTCAAAC
CTTGCCAGATTCGACGGTGTCAAATACGGATACCGTACAAAGGAGTACACAGACCTTCACAACATGTACAAGAAATCGCG
TTCTGAGGGCTTTGGACCTGAGGTAAAGAGAAGAATCATGCTCGGCTCCTTCGTACTCTCATCAGGATATTATGATGCAT
ATTACTTAAAGGCATTAAGGGTAAAAGCACTTATCAAAAAAGCCTTTGATGATGCCTTTGCCAAATATGATGTGATACTC
GGACCGGCAGCACCAACCACAGCTCCAAAGCTTGGAGAGAGCTTAAGTGATCCAATCCAGATGTACCTTGGTGATATCTA
CACCATATCTGTAAACCTTGCAGGTCTTCCGGGAATATCGCTTCCGTGCGGCATGGACAAAAACGGACTTCCAATCGGAT
TACAGCTCATCGGAGACTGCTTTAAGGAGAAGAATATCATCCGCGCAGCCTACAGCTTTGAAAAAACAAGAGAGCTTAAG
CGCAGCACTATTGCGGAGGAATACAGTAACAAAAATACAGCAGATACCAATAAATCTGCCGGTGCACAGTAG

Upstream 100 bases:

>100_bases
GTGACGGAAGTGAAGCAACACTTGCCAATGCACCGGTCAAGAAGGACGGCGGATTCAAGGTACCAAAGACAATCGGCGAA
TAACGGCAAGGAGGAGAAAA

Downstream 100 bases:

>100_bases
GAAGGAGGCAAAAAGGATATGAAAAACTATGAGACAGTCATCGGACTTGAGGTCCATGTAGAGTTAGCTACAAAAACAAA
GATCTTTTGCGCCTGCTCCA

Product: glutamyl-tRNA(Gln) amidotransferase, A subunit

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MDLMSLTAVELGKKIQAKEVTVEEAVKAAIASIKAKEEKINSFVTIDEEGALKKAAEVQAKIDAGELKGALAGVPVAIKD
NMCTEGLLTTCSSKILYNFIPTYTAEAVKRLEDAGCVIVGKTNMDEFAMGSTTETSAFGATKNPWNTEHVPGGSSGGSCA
AVAAEEVPFALGSDTGGSIRQPSSFCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPIAKDVTDCAIILEAIASYDTKDSTS
VNRDDLKFTEALVDDVKGMKIGIPKDYLGDGLDPEVKSAILAAADELKKKGAVVEEFDLGLVEYAIPAYYVIACAEASSN
LARFDGVKYGYRTKEYTDLHNMYKKSRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRVKALIKKAFDDAFAKYDVIL
GPAAPTTAPKLGESLSDPIQMYLGDIYTISVNLAGLPGISLPCGMDKNGLPIGLQLIGDCFKEKNIIRAAYSFEKTRELK
RSTIAEEYSNKNTADTNKSAGAQ

Sequences:

>Translated_503_residues
MDLMSLTAVELGKKIQAKEVTVEEAVKAAIASIKAKEEKINSFVTIDEEGALKKAAEVQAKIDAGELKGALAGVPVAIKD
NMCTEGLLTTCSSKILYNFIPTYTAEAVKRLEDAGCVIVGKTNMDEFAMGSTTETSAFGATKNPWNTEHVPGGSSGGSCA
AVAAEEVPFALGSDTGGSIRQPSSFCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPIAKDVTDCAIILEAIASYDTKDSTS
VNRDDLKFTEALVDDVKGMKIGIPKDYLGDGLDPEVKSAILAAADELKKKGAVVEEFDLGLVEYAIPAYYVIACAEASSN
LARFDGVKYGYRTKEYTDLHNMYKKSRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRVKALIKKAFDDAFAKYDVIL
GPAAPTTAPKLGESLSDPIQMYLGDIYTISVNLAGLPGISLPCGMDKNGLPIGLQLIGDCFKEKNIIRAAYSFEKTRELK
RSTIAEEYSNKNTADTNKSAGAQ
>Mature_503_residues
MDLMSLTAVELGKKIQAKEVTVEEAVKAAIASIKAKEEKINSFVTIDEEGALKKAAEVQAKIDAGELKGALAGVPVAIKD
NMCTEGLLTTCSSKILYNFIPTYTAEAVKRLEDAGCVIVGKTNMDEFAMGSTTETSAFGATKNPWNTEHVPGGSSGGSCA
AVAAEEVPFALGSDTGGSIRQPSSFCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPIAKDVTDCAIILEAIASYDTKDSTS
VNRDDLKFTEALVDDVKGMKIGIPKDYLGDGLDPEVKSAILAAADELKKKGAVVEEFDLGLVEYAIPAYYVIACAEASSN
LARFDGVKYGYRTKEYTDLHNMYKKSRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRVKALIKKAFDDAFAKYDVIL
GPAAPTTAPKLGESLSDPIQMYLGDIYTISVNLAGLPGISLPCGMDKNGLPIGLQLIGDCFKEKNIIRAAYSFEKTRELK
RSTIAEEYSNKNTADTNKSAGAQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=38.3673469387755, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=26.1044176706827, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI166795287, Length=260, Percent_Identity=30, Blast_Score=72, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=425, Percent_Identity=39.2941176470588, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=27, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71990152, Length=319, Percent_Identity=28.8401253918495, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=311, Percent_Identity=25.7234726688103, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=326, Percent_Identity=26.6871165644172, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=251, Percent_Identity=30.6772908366534, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=251, Percent_Identity=30.6772908366534, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=327, Percent_Identity=26.2996941896024, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=460, Percent_Identity=33.9130434782609, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=446, Percent_Identity=29.8206278026906, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=234, Percent_Identity=29.9145299145299, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=38.877755511022, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=25.8196721311475, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=25.8196721311475, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=25.8196721311475, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=25.8196721311475, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=27.9116465863454, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24644968, Length=500, Percent_Identity=25.8, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356731, Length=488, Percent_Identity=23.9754098360656, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648435, Length=484, Percent_Identity=24.3801652892562, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648437, Length=484, Percent_Identity=24.3801652892562, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550774, Length=484, Percent_Identity=24.3801652892562, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648441, Length=424, Percent_Identity=23.1132075471698, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648439, Length=424, Percent_Identity=23.1132075471698, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_EUBR3 (C4ZHB9)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937065.1
- GeneID:   7964182
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1169
- ProtClustDB:   CLSK2506100
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53724; Mature: 53724

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLMSLTAVELGKKIQAKEVTVEEAVKAAIASIKAKEEKINSFVTIDEEGALKKAAEVQA
CCCHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
KIDAGELKGALAGVPVAIKDNMCTEGLLTTCSSKILYNFIPTYTAEAVKRLEDAGCVIVG
HCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEE
KTNMDEFAMGSTTETSAFGATKNPWNTEHVPGGSSGGSCAAVAAEEVPFALGSDTGGSIR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCEEECCCCCCCCC
QPSSFCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPIAKDVTDCAIILEAIASYDTKDSTS
CCCHHCCCCCCCCCCCCHHHCEEEEECCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCC
VNRDDLKFTEALVDDVKGMKIGIPKDYLGDGLDPEVKSAILAAADELKKKGAVVEEFDLG
CCHHHHHHHHHHHHHCCCCEECCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCH
LVEYAIPAYYVIACAEASSNLARFDGVKYGYRTKEYTDLHNMYKKSRSEGFGPEVKRRIM
HHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
LGSFVLSSGYYDAYYLKALRVKALIKKAFDDAFAKYDVILGPAAPTTAPKLGESLSDPIQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHH
MYLGDIYTISVNLAGLPGISLPCGMDKNGLPIGLQLIGDCFKEKNIIRAAYSFEKTRELK
HHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RSTIAEEYSNKNTADTNKSAGAQ
HHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLMSLTAVELGKKIQAKEVTVEEAVKAAIASIKAKEEKINSFVTIDEEGALKKAAEVQA
CCCHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
KIDAGELKGALAGVPVAIKDNMCTEGLLTTCSSKILYNFIPTYTAEAVKRLEDAGCVIVG
HCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEE
KTNMDEFAMGSTTETSAFGATKNPWNTEHVPGGSSGGSCAAVAAEEVPFALGSDTGGSIR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCEEECCCCCCCCC
QPSSFCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPIAKDVTDCAIILEAIASYDTKDSTS
CCCHHCCCCCCCCCCCCHHHCEEEEECCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCC
VNRDDLKFTEALVDDVKGMKIGIPKDYLGDGLDPEVKSAILAAADELKKKGAVVEEFDLG
CCHHHHHHHHHHHHHCCCCEECCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCH
LVEYAIPAYYVIACAEASSNLARFDGVKYGYRTKEYTDLHNMYKKSRSEGFGPEVKRRIM
HHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
LGSFVLSSGYYDAYYLKALRVKALIKKAFDDAFAKYDVILGPAAPTTAPKLGESLSDPIQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHH
MYLGDIYTISVNLAGLPGISLPCGMDKNGLPIGLQLIGDCFKEKNIIRAAYSFEKTRELK
HHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RSTIAEEYSNKNTADTNKSAGAQ
HHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA