Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 238923507

GI number: 238923507

Start: 1038919

End: 1040193

Strand: Direct

Name: purD [H]

Synonym: EUBREC_1127

Alternate gene names: 238923507

Gene position: 1038919-1040193 (Clockwise)

Preceding gene: 238923506

Following gene: 238923508

Centisome position: 30.12

GC content: 45.8

Gene sequence:

>1275_bases
ATGAAGGTATTAGTAGTAGGAAGCGGCGGAAGAGAACATGCAATATGCAGGGCTGTTGCAAAGAGCTCACGCGTTGACAA
GATATACTGTGCACCGGGCAATGCAGGTATCGCTGCACTTGCAGAGTGTGTGCCAATCGGTGCGATGGAGTTTGATAAGC
TCGTATCATTTGCAAAGGATAATGCCATTGATTTTACAATCATCGGCATGGATGATCCGCTTGTAGGTGGTATCGTTGAC
GAGTTCGAAAAGGCAGGGCTCAAGTGTTTCGGACCAAAGAAGAATGCAGCTATTCTTGAGGGCTCAAAGGCATTTTCAAA
GGATCTCATGAAAAAGTATAATATCCCTACTGCGGCATATGAGAATTTTACTGATGCCAAAGAGGCACTTAAATATTTAG
AGACAGCAAAGTTCCCTATCGTGTTAAAGGCAGACGGACTTGCTCTCGGAAAGGGTGTGCTTATCTGCAATACTCTTGAG
GAGGCAAAAGAGGGTGTCAGAACCATCATGGAGGACAAGAAGTTCGGAACAGCCGGAAACACCATGGTAGTTGAGGAGTT
CATGACGGGCCGTGAGGTTTCAGTGCTTTCATTTGTAGACGGAAAGACTATCAAGATTATGTCATCTGCGCAGGACCACA
AGCGTGCAGGCGATGGAGATACAGGCCTTAATACAGGCGGCATGGGTAATTTCTCACCTAGCCCGTTCTATACCAATGAG
GTCGATGATTTCTGCAAGAAGTATATTTATCAGGCAACTGTAGATGCTATGGCTAAAGAGGGCAGACCTTTTACAGGAGT
TATCTTCTTCGGACTCATGCTCACAGAGGATGGACCAAAGGTGCTTGAGTACAATGCAAGATTCGGAGATCCTGAGGCAC
AGGTTGTGCTTCCTCGTATGAAGAATGATATCATTGATGTAATGGAGGCCTGTGTAGACGGAACTCTTGACACAATAGAT
CTTCAGTTTGAGGACAATGCATGCGTATGCGTGGTTCTGGCATCAGACGGATATCCTGTCAAGTATGAGAAGGGCTTTAA
GATAACTGGCTTTGAGAAGTTTGACGGCAAAGAGGATTATTACTGCTATCATGCCGGCACCAAATTTGATGCTGACGGCA
ATATCGTCACAAACGGAGGACGAGTGCTTGGTATCACTGCAACAGGCGCCACACTCAAGGAGGCCAGAGCCAAGGCTTAC
GAGGCAACAGAGTGGGTACAGTTTGATAATAAATACATGAGACATGATATCGGAAAGGCTATAGATGAAGCATAA

Upstream 100 bases:

>100_bases
AGTAATGACAGTGCTGTAAGTAATTAGTATGTAAACACAGGTAGATTATGATTACCGGACAATGAAAAGCTTATGTATAG
CTTAGGTATAGGAGGCAAAA

Downstream 100 bases:

>100_bases
GGGTATTATTTTTGATATGGATGGCACTCTCTGGGATTCAGCAGAGAATGTGGCAAAATCATGGAATAAGGCCATAGCAG
ATTATGGCTATGAGAGGGCA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKVLVVGSGGREHAICRAVAKSSRVDKIYCAPGNAGIAALAECVPIGAMEFDKLVSFAKDNAIDFTIIGMDDPLVGGIVD
EFEKAGLKCFGPKKNAAILEGSKAFSKDLMKKYNIPTAAYENFTDAKEALKYLETAKFPIVLKADGLALGKGVLICNTLE
EAKEGVRTIMEDKKFGTAGNTMVVEEFMTGREVSVLSFVDGKTIKIMSSAQDHKRAGDGDTGLNTGGMGNFSPSPFYTNE
VDDFCKKYIYQATVDAMAKEGRPFTGVIFFGLMLTEDGPKVLEYNARFGDPEAQVVLPRMKNDIIDVMEACVDGTLDTID
LQFEDNACVCVVLASDGYPVKYEKGFKITGFEKFDGKEDYYCYHAGTKFDADGNIVTNGGRVLGITATGATLKEARAKAY
EATEWVQFDNKYMRHDIGKAIDEA

Sequences:

>Translated_424_residues
MKVLVVGSGGREHAICRAVAKSSRVDKIYCAPGNAGIAALAECVPIGAMEFDKLVSFAKDNAIDFTIIGMDDPLVGGIVD
EFEKAGLKCFGPKKNAAILEGSKAFSKDLMKKYNIPTAAYENFTDAKEALKYLETAKFPIVLKADGLALGKGVLICNTLE
EAKEGVRTIMEDKKFGTAGNTMVVEEFMTGREVSVLSFVDGKTIKIMSSAQDHKRAGDGDTGLNTGGMGNFSPSPFYTNE
VDDFCKKYIYQATVDAMAKEGRPFTGVIFFGLMLTEDGPKVLEYNARFGDPEAQVVLPRMKNDIIDVMEACVDGTLDTID
LQFEDNACVCVVLASDGYPVKYEKGFKITGFEKFDGKEDYYCYHAGTKFDADGNIVTNGGRVLGITATGATLKEARAKAY
EATEWVQFDNKYMRHDIGKAIDEA
>Mature_424_residues
MKVLVVGSGGREHAICRAVAKSSRVDKIYCAPGNAGIAALAECVPIGAMEFDKLVSFAKDNAIDFTIIGMDDPLVGGIVD
EFEKAGLKCFGPKKNAAILEGSKAFSKDLMKKYNIPTAAYENFTDAKEALKYLETAKFPIVLKADGLALGKGVLICNTLE
EAKEGVRTIMEDKKFGTAGNTMVVEEFMTGREVSVLSFVDGKTIKIMSSAQDHKRAGDGDTGLNTGGMGNFSPSPFYTNE
VDDFCKKYIYQATVDAMAKEGRPFTGVIFFGLMLTEDGPKVLEYNARFGDPEAQVVLPRMKNDIIDVMEACVDGTLDTID
LQFEDNACVCVVLASDGYPVKYEKGFKITGFEKFDGKEDYYCYHAGTKFDADGNIVTNGGRVLGITATGATLKEARAKAY
EATEWVQFDNKYMRHDIGKAIDEA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=421, Percent_Identity=44.1805225653207, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI4503915, Length=421, Percent_Identity=44.1805225653207, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI209869995, Length=421, Percent_Identity=44.1805225653207, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI209869993, Length=421, Percent_Identity=44.1805225653207, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=46.4953271028037, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567511, Length=429, Percent_Identity=39.3939393939394, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6321203, Length=435, Percent_Identity=41.8390804597701, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI62471619, Length=427, Percent_Identity=43.7939110070258, Blast_Score=344, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24582400, Length=414, Percent_Identity=44.4444444444444, Blast_Score=342, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46060; Mature: 46060

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGSGGREHAICRAVAKSSRVDKIYCAPGNAGIAALAECVPIGAMEFDKLVSFAKD
CEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCC
NAIDFTIIGMDDPLVGGIVDEFEKAGLKCFGPKKNAAILEGSKAFSKDLMKKYNIPTAAY
CCEEEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCEEEECCHHHHHHHHHHHCCCHHHH
ENFTDAKEALKYLETAKFPIVLKADGLALGKGVLICNTLEEAKEGVRTIMEDKKFGTAGN
CCHHHHHHHHHHHHHCCCCEEEEECCCEECCCEEEECCHHHHHHHHHHHHHCCCCCCCCC
TMVVEEFMTGREVSVLSFVDGKTIKIMSSAQDHKRAGDGDTGLNTGGMGNFSPSPFYTNE
EEEHHHHHCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VDDFCKKYIYQATVDAMAKEGRPFTGVIFFGLMLTEDGPKVLEYNARFGDPEAQVVLPRM
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCEEECCHH
KNDIIDVMEACVDGTLDTIDLQFEDNACVCVVLASDGYPVKYEKGFKITGFEKFDGKEDY
HHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCEEECCCEEEECCCCCCCCCCE
YCYHAGTKFDADGNIVTNGGRVLGITATGATLKEARAKAYEATEWVQFDNKYMRHDIGKA
EEEECCCEECCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDEA
HCCC
>Mature Secondary Structure
MKVLVVGSGGREHAICRAVAKSSRVDKIYCAPGNAGIAALAECVPIGAMEFDKLVSFAKD
CEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCC
NAIDFTIIGMDDPLVGGIVDEFEKAGLKCFGPKKNAAILEGSKAFSKDLMKKYNIPTAAY
CCEEEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCEEEECCHHHHHHHHHHHCCCHHHH
ENFTDAKEALKYLETAKFPIVLKADGLALGKGVLICNTLEEAKEGVRTIMEDKKFGTAGN
CCHHHHHHHHHHHHHCCCCEEEEECCCEECCCEEEECCHHHHHHHHHHHHHCCCCCCCCC
TMVVEEFMTGREVSVLSFVDGKTIKIMSSAQDHKRAGDGDTGLNTGGMGNFSPSPFYTNE
EEEHHHHHCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VDDFCKKYIYQATVDAMAKEGRPFTGVIFFGLMLTEDGPKVLEYNARFGDPEAQVVLPRM
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCEEECCHH
KNDIIDVMEACVDGTLDTIDLQFEDNACVCVVLASDGYPVKYEKGFKITGFEKFDGKEDY
HHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCEEECCCEEEECCCCCCCCCCE
YCYHAGTKFDADGNIVTNGGRVLGITATGATLKEARAKAYEATEWVQFDNKYMRHDIGKA
EEEECCCEECCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDEA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]