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The map label for this gene is ppsA [H]

Identifier: 238923407

GI number: 238923407

Start: 922325

End: 923743

Strand: Direct

Name: ppsA [H]

Synonym: EUBREC_1027

Alternate gene names: 238923407

Gene position: 922325-923743 (Clockwise)

Preceding gene: 238923406

Following gene: 238923408

Centisome position: 26.74

GC content: 44.89

Gene sequence:

>1419_bases
ATGATGAAGACGAGAAAGGGACACAACGTATATCCGATTACGGTTGCACAAAAATTTCATTTGTACTATGCAAAGTACTG
TCCGAATATGGCAGTACTCAATATAGGTACAAGCCTGACAATCGGCACGGAGCTTGACTGGAACGTGCTAAGGGATTCAA
TCAACTACGCGTATGCCAGAAACGAGGCCATGCGTATCCGCTTTACAAGGGATAAGGATGGAGAGTGCTATCAGTATATT
GCGGATGTGGATGAGGATTTTAAAGAGAGAACTGTCGATTTCAAGGATTTTACGGATGTAACCATGGAGGAGGCAGAGAA
TGAGATGCAAGGCTGGACACAGGTGCCGTTTGAGTTTGAGGACTCTCCTATGACCAAAATCGTGATGATAAAGATGCCGG
ATGGATTCAATGGAGTGTACTTCTTAGGTCATCACATGGTGGTTGATGCGCAGTCACTTATTGCATTTTTAAAGGATATT
ATTGAGATATACTGTAATGCCATGTATGAGGGAGTACCTTTTCCAAAGGATATGTGCTCATATATCGAGCAGCTTAAAAA
GGATCTGGCATATGAAGCCGGAAGTAAGGCACAGCTTAGGGACAGAGAGTTCTTTGAGAAGCTTATCAGACAGTCGGAGC
CAATCTACAACGGAATCGACGGCACAGCAAAGCTTGATGCGGCAAGAGAACTGATGCATGATAACAAGCTAAGGAGTGCA
TTCAATGCATCGGATGATGTCACCTCAGCGCTTGATATCTTTCATCTGGAGGCTGAGCCGACAAAGCGTCTTATGGATTT
TTGCGAGAAATATCATATTTCGCTTGCTTGTCTGCTTCTCATGGGAATCAGGACGTTTTTCCAGAAGGAAAACGGCTTCG
ATGATGTCTCCGTAAACAATGCCATCGCCAGACGTGCGACACTTAAGGAGAAGAAGTCAGGCGGCACACGTATTCATTCG
TTTCCGTTTAGGACGTGCTTTTCAAAGGATGTACGCTTTATCGATGCTGTGTATACCATTAGGGATAAGCAAAACGAGCT
GTTCCGCCATGCAAACTACAACCCGACAGAGTACTTTGCACTGCGCTCAAAGACCTATCCGCAGCCAAAGGCGGGGCTTA
CATATGAGCCGATGTCGCTTACATATCAGCCTATGACCTTAAAGGAAAAGGGCTTAAACGACCTGGGAGATATAAAATAC
AAGACTAAGTGGTACCCGAACGGCATGACTACACAGGCTATGTATCTAACGGTAATGCACCGCCCGGAGGACAACGGACT
GGATTTCAGCTTCGAGCATCAGGTCAAGGCAGTCTCAAGAAAGCAGCTTGAGTATATGTACTATTACCTGTGCAAAATCA
TGTTCAAGGGAGCAGAAAACCCTGAGCTTACAATAGGCGAAATCATTAAATTAGTGTAG

Upstream 100 bases:

>100_bases
CAAAGCTGATAGTAAAGGGTGCAGGACACTGCGAGAGCTATTACAAGAATACGAAAGCTTTCGAGGACGCACTTGATAAA
TTTTTGGAAGGAGTTATGAG

Downstream 100 bases:

>100_bases
TGGATTAGGAAACGTATTAGGTAAAAGGAGATTATTATGTTTGATGAATTAGTGGAAATTATCTGTAATTATGTTGATGT
TCAGCCGGCAGATGTGCACG

Product: non-ribosomal peptide synthetase, terminal component

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Peptide synthase 1; ATP-dependent glutamate adenylase 1; GluA 1; Glutamate activase 1; ATP-dependent ornithine adenylase; OrnA; Ornithine activase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MMKTRKGHNVYPITVAQKFHLYYAKYCPNMAVLNIGTSLTIGTELDWNVLRDSINYAYARNEAMRIRFTRDKDGECYQYI
ADVDEDFKERTVDFKDFTDVTMEEAENEMQGWTQVPFEFEDSPMTKIVMIKMPDGFNGVYFLGHHMVVDAQSLIAFLKDI
IEIYCNAMYEGVPFPKDMCSYIEQLKKDLAYEAGSKAQLRDREFFEKLIRQSEPIYNGIDGTAKLDAARELMHDNKLRSA
FNASDDVTSALDIFHLEAEPTKRLMDFCEKYHISLACLLLMGIRTFFQKENGFDDVSVNNAIARRATLKEKKSGGTRIHS
FPFRTCFSKDVRFIDAVYTIRDKQNELFRHANYNPTEYFALRSKTYPQPKAGLTYEPMSLTYQPMTLKEKGLNDLGDIKY
KTKWYPNGMTTQAMYLTVMHRPEDNGLDFSFEHQVKAVSRKQLEYMYYYLCKIMFKGAENPELTIGEIIKLV

Sequences:

>Translated_472_residues
MMKTRKGHNVYPITVAQKFHLYYAKYCPNMAVLNIGTSLTIGTELDWNVLRDSINYAYARNEAMRIRFTRDKDGECYQYI
ADVDEDFKERTVDFKDFTDVTMEEAENEMQGWTQVPFEFEDSPMTKIVMIKMPDGFNGVYFLGHHMVVDAQSLIAFLKDI
IEIYCNAMYEGVPFPKDMCSYIEQLKKDLAYEAGSKAQLRDREFFEKLIRQSEPIYNGIDGTAKLDAARELMHDNKLRSA
FNASDDVTSALDIFHLEAEPTKRLMDFCEKYHISLACLLLMGIRTFFQKENGFDDVSVNNAIARRATLKEKKSGGTRIHS
FPFRTCFSKDVRFIDAVYTIRDKQNELFRHANYNPTEYFALRSKTYPQPKAGLTYEPMSLTYQPMTLKEKGLNDLGDIKY
KTKWYPNGMTTQAMYLTVMHRPEDNGLDFSFEHQVKAVSRKQLEYMYYYLCKIMFKGAENPELTIGEIIKLV
>Mature_472_residues
MMKTRKGHNVYPITVAQKFHLYYAKYCPNMAVLNIGTSLTIGTELDWNVLRDSINYAYARNEAMRIRFTRDKDGECYQYI
ADVDEDFKERTVDFKDFTDVTMEEAENEMQGWTQVPFEFEDSPMTKIVMIKMPDGFNGVYFLGHHMVVDAQSLIAFLKDI
IEIYCNAMYEGVPFPKDMCSYIEQLKKDLAYEAGSKAQLRDREFFEKLIRQSEPIYNGIDGTAKLDAARELMHDNKLRSA
FNASDDVTSALDIFHLEAEPTKRLMDFCEKYHISLACLLLMGIRTFFQKENGFDDVSVNNAIARRATLKEKKSGGTRIHS
FPFRTCFSKDVRFIDAVYTIRDKQNELFRHANYNPTEYFALRSKTYPQPKAGLTYEPMSLTYQPMTLKEKGLNDLGDIKY
KTKWYPNGMTTQAMYLTVMHRPEDNGLDFSFEHQVKAVSRKQLEYMYYYLCKIMFKGAENPELTIGEIIKLV

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Glu and Orn as part of the biosynthesis of the lipopeptide antibiotic lipastatin. The Orn residue is further epimerized to the D-isomer form. The activation sites fo

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 54943; Mature: 54943

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTRKGHNVYPITVAQKFHLYYAKYCPNMAVLNIGTSLTIGTELDWNVLRDSINYAYAR
CCCCCCCCCEEEEEEHHHHHHHHHHHCCCEEEEECCCEEEECCCCCHHHHHHHHHHHEEC
NEAMRIRFTRDKDGECYQYIADVDEDFKERTVDFKDFTDVTMEEAENEMQGWTQVPFEFE
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECCCEEC
DSPMTKIVMIKMPDGFNGVYFLGHHMVVDAQSLIAFLKDIIEIYCNAMYEGVPFPKDMCS
CCCCEEEEEEECCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YIEQLKKDLAYEAGSKAQLRDREFFEKLIRQSEPIYNGIDGTAKLDAARELMHDNKLRSA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FNASDDVTSALDIFHLEAEPTKRLMDFCEKYHISLACLLLMGIRTFFQKENGFDDVSVNN
CCCCCHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AIARRATLKEKKSGGTRIHSFPFRTCFSKDVRFIDAVYTIRDKQNELFRHANYNPTEYFA
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHEEE
LRSKTYPQPKAGLTYEPMSLTYQPMTLKEKGLNDLGDIKYKTKWYPNGMTTQAMYLTVMH
ECCCCCCCCCCCCEECCCCEEECCCCHHHCCCCCHHCCCEEEEECCCCCCCCEEEEEEEE
RPEDNGLDFSFEHQVKAVSRKQLEYMYYYLCKIMFKGAENPELTIGEIIKLV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCC
>Mature Secondary Structure
MMKTRKGHNVYPITVAQKFHLYYAKYCPNMAVLNIGTSLTIGTELDWNVLRDSINYAYAR
CCCCCCCCCEEEEEEHHHHHHHHHHHCCCEEEEECCCEEEECCCCCHHHHHHHHHHHEEC
NEAMRIRFTRDKDGECYQYIADVDEDFKERTVDFKDFTDVTMEEAENEMQGWTQVPFEFE
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECCCEEC
DSPMTKIVMIKMPDGFNGVYFLGHHMVVDAQSLIAFLKDIIEIYCNAMYEGVPFPKDMCS
CCCCEEEEEEECCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YIEQLKKDLAYEAGSKAQLRDREFFEKLIRQSEPIYNGIDGTAKLDAARELMHDNKLRSA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FNASDDVTSALDIFHLEAEPTKRLMDFCEKYHISLACLLLMGIRTFFQKENGFDDVSVNN
CCCCCHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AIARRATLKEKKSGGTRIHSFPFRTCFSKDVRFIDAVYTIRDKQNELFRHANYNPTEYFA
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHEEE
LRSKTYPQPKAGLTYEPMSLTYQPMTLKEKGLNDLGDIKYKTKWYPNGMTTQAMYLTVMH
ECCCCCCCCCCCCEECCCCEEECCCCHHHCCCCCHHCCCEEEEECCCCCCCCEEEEEEEE
RPEDNGLDFSFEHQVKAVSRKQLEYMYYYLCKIMFKGAENPELTIGEIIKLV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377; 10471562 [H]