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The map label for this gene is bioB [H]

Identifier: 238923303

GI number: 238923303

Start: 821121

End: 822134

Strand: Direct

Name: bioB [H]

Synonym: EUBREC_0920

Alternate gene names: 238923303

Gene position: 821121-822134 (Clockwise)

Preceding gene: 238923302

Following gene: 238923304

Centisome position: 23.8

GC content: 45.17

Gene sequence:

>1014_bases
ATGAGAGAAGAAATACAGGACACGAAGGAGATTAAAATGGATCCAATAACACTGGCACAGGAGATAATAGACGGAAGAAG
AATCACAAGGGATGATGACCTATCATTTTTCCTTACATGTGATTTAGACGAGCTGTGTAAGGGCGCAGATATGATCAGGG
AGAAGTTTATCGGTGACAAGGTAGATTTGTGCTCGATAATAAACGGAAGAAGCGGAAAATGCCCTGAGGACTGCAAATAC
TGTGCGCAGTCAGCACATAATCATACAAATTGTGAGGTTTATGATTTTTTACCTGAGGAAAAGATACTTGAGGCTTGCAG
GATGAATGAGAGAGAGGGTGTAGACCGTTTCTCGATAGTTACAGCGGGAAAAGCGCTGACAGGTGAGGAATTTGACAAGG
CAATACATGCATATGAGACTATGAAAAGAGAGTGTAAGATTGATTTATGTGCATCAATGGGCTTTCTTTCAGCAGAGCAG
CTTCACAGACTGCACGAGGCAGGTGTCAGCAGCTATCACCACAATATAGAGACCTCAAAGCGTAATTTCCCCAATATATG
CACCACACATACCTATGATATGAAGATTGAGACGCTTAAAAAGGTGAAGGCAGAGGGCATGTATGCGTGCTCAGGCGGCA
TCATCGGAATGGGTGAGACCTGGGAGGACAGGCTTGATATGGCAGTAAGCCTTGCAGAGGTAGGAGTGGATTCCATTCCG
CTTAATGCGCTTATGCCGATTGCAGGCACTCCGCTTGAGGGACTTGAGCATATCAGTGAGCCTGATATACTGCGTACAGT
GTCATTTTTCAGATATATAAATCCAAAGGCTGACATACGTCTCGGAGCCGGAAGAGCGCTGCTTACAAATGACGGAGAGC
TTGCATTCAAGTCGGGAGCATCAGCCACAATTACAGGAAACATGCTTACGACAGTAGCGTGTGCCACAATCAGAAGTGAC
AAGGAAATGCTTGAGGGCATGGGCAGGAATGTGACACCTGAGTACATGAAATAA

Upstream 100 bases:

>100_bases
TCTTGACGTTTTTCTGTTTTTCTATTATCATTGTTAACCGAGGAAATATTTATAGTTGACAATTATAACGGATGATATGT
ATTATAATGATACTTATTAA

Downstream 100 bases:

>100_bases
AGGTAATTATTCAGAATATAAAATATGTCAAAGATGCGTAAAATAGTATTAGTTTTGCGCATCTTTTTTAGTACAAATGA
CAGGATTGGTTGCGGGGTGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MREEIQDTKEIKMDPITLAQEIIDGRRITRDDDLSFFLTCDLDELCKGADMIREKFIGDKVDLCSIINGRSGKCPEDCKY
CAQSAHNHTNCEVYDFLPEEKILEACRMNEREGVDRFSIVTAGKALTGEEFDKAIHAYETMKRECKIDLCASMGFLSAEQ
LHRLHEAGVSSYHHNIETSKRNFPNICTTHTYDMKIETLKKVKAEGMYACSGGIIGMGETWEDRLDMAVSLAEVGVDSIP
LNALMPIAGTPLEGLEHISEPDILRTVSFFRYINPKADIRLGAGRALLTNDGELAFKSGASATITGNMLTTVACATIRSD
KEMLEGMGRNVTPEYMK

Sequences:

>Translated_337_residues
MREEIQDTKEIKMDPITLAQEIIDGRRITRDDDLSFFLTCDLDELCKGADMIREKFIGDKVDLCSIINGRSGKCPEDCKY
CAQSAHNHTNCEVYDFLPEEKILEACRMNEREGVDRFSIVTAGKALTGEEFDKAIHAYETMKRECKIDLCASMGFLSAEQ
LHRLHEAGVSSYHHNIETSKRNFPNICTTHTYDMKIETLKKVKAEGMYACSGGIIGMGETWEDRLDMAVSLAEVGVDSIP
LNALMPIAGTPLEGLEHISEPDILRTVSFFRYINPKADIRLGAGRALLTNDGELAFKSGASATITGNMLTTVACATIRSD
KEMLEGMGRNVTPEYMK
>Mature_337_residues
MREEIQDTKEIKMDPITLAQEIIDGRRITRDDDLSFFLTCDLDELCKGADMIREKFIGDKVDLCSIINGRSGKCPEDCKY
CAQSAHNHTNCEVYDFLPEEKILEACRMNEREGVDRFSIVTAGKALTGEEFDKAIHAYETMKRECKIDLCASMGFLSAEQ
LHRLHEAGVSSYHHNIETSKRNFPNICTTHTYDMKIETLKKVKAEGMYACSGGIIGMGETWEDRLDMAVSLAEVGVDSIP
LNALMPIAGTPLEGLEHISEPDILRTVSFFRYINPKADIRLGAGRALLTNDGELAFKSGASATITGNMLTTVACATIRSD
KEMLEGMGRNVTPEYMK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=295, Percent_Identity=32.5423728813559, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321725, Length=300, Percent_Identity=33, Blast_Score=175, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37545; Mature: 37545

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREEIQDTKEIKMDPITLAQEIIDGRRITRDDDLSFFLTCDLDELCKGADMIREKFIGDK
CCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCH
VDLCSIINGRSGKCPEDCKYCAQSAHNHTNCEVYDFLPEEKILEACRMNEREGVDRFSIV
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCEEEEE
TAGKALTGEEFDKAIHAYETMKRECKIDLCASMGFLSAEQLHRLHEAGVSSYHHNIETSK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
RNFPNICTTHTYDMKIETLKKVKAEGMYACSGGIIGMGETWEDRLDMAVSLAEVGVDSIP
CCCCCCCCCCEECCHHHHHHHHHHCCEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCC
LNALMPIAGTPLEGLEHISEPDILRTVSFFRYINPKADIRLGAGRALLTNDGELAFKSGA
HHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCEEEECCC
SATITGNMLTTVACATIRSDKEMLEGMGRNVTPEYMK
CEEEECCHHHHHHHHHHHCHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure
MREEIQDTKEIKMDPITLAQEIIDGRRITRDDDLSFFLTCDLDELCKGADMIREKFIGDK
CCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCH
VDLCSIINGRSGKCPEDCKYCAQSAHNHTNCEVYDFLPEEKILEACRMNEREGVDRFSIV
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCEEEEE
TAGKALTGEEFDKAIHAYETMKRECKIDLCASMGFLSAEQLHRLHEAGVSSYHHNIETSK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
RNFPNICTTHTYDMKIETLKKVKAEGMYACSGGIIGMGETWEDRLDMAVSLAEVGVDSIP
CCCCCCCCCCEECCHHHHHHHHHHCCEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCC
LNALMPIAGTPLEGLEHISEPDILRTVSFFRYINPKADIRLGAGRALLTNDGELAFKSGA
HHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCEEEECCC
SATITGNMLTTVACATIRSDKEMLEGMGRNVTPEYMK
CEEEECCHHHHHHHHHHHCHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA