Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 238923295

GI number: 238923295

Start: 810379

End: 811665

Strand: Direct

Name: mdeA [H]

Synonym: EUBREC_0906

Alternate gene names: 238923295

Gene position: 810379-811665 (Clockwise)

Preceding gene: 238923294

Following gene: 238923296

Centisome position: 23.49

GC content: 47.32

Gene sequence:

>1287_bases
ATGAAAGAATACAGTATAGGTACAAAGTGTGTACAGGCAGGCTATGCAGCCGGAAACGGAGAGCCGAGACAGATTCCGAT
TATTCAGTCAACTACATTCAAATATGACACAAGCGAGGACATGGGAAAGCTTTTTGACCTTGAGGCAGAGGGCTATTTCT
ACAGCAGACTGCAGAATCCAACCTGTGATACGGTAGCAAGAAAAATCGCAGCTATGGAGGGTGGCACTGCTGCCATGCTC
ACATCATCAGGACAGGCAGCCAATTTCTATGCCATGTTCAACATATGTGAGTGCGGAGACCATATCGTGGCATCATCATC
CATCTATGGAGGCACCTTCAACCTCATCGCGGTAACTATGGCGAAGATGGGTATTACAGCTACATTCGTATCTCCTGATG
CTACAGAGGAGGAGCTGAATGCAGCATTCAGACCAAACACAAAGCTTATGTTTGGTGAGACTATTGCCAATCCTGCGCTT
ACGGTGCTTGATATAGAGCTTTTTGCAAAGGTGGCACACGAGCACGGAGTACCGCTTATAGTGGACAATACCTTCCCGAC
ACCTGTAAACTGCAGACCAATTGAGTGGGGCGCAGATATTGTCACACACTCTACCACAAAGTACATGGACGGACACGGAG
CCACACTCGGCGGAGCAATCGTAGACAGCGGCAAATTCGACTGGATGGCACATGCAGATAAATATCCGGGACTGTGCACA
CCTGATGACAGCTATCATGGCATCACATATGCAGAGAAATTCGGCAAGGAAGGTGCGTTCATCACCAAATGTACAGCACA
GCTTATGCGCGATTTTGGTTCTATGCAGTCACCACAGAATGCATTTATGCTGAATCTTGGCCTTGAGTCACTTCATGTGC
GTATGCCAAAGCATGTGGAAAATGGACAGGCAGTGGCTGAATTTTTGGAAAATCATCCAAAGGTTGCCTATGTAAACTAC
TCAGGGCTTCCTTCAAACAAATACTATGAGAGAGCACAGAAGTATCTTCCAAACGGAGGCTGCGGAGTCGTATCATTTGG
CTTAAAGGGCGGCAGAGAGGCAGCGTCTGCATTTATGAAGGCATTAAGGCTTGGTGCAATCGAGACACATGTGGCAGATG
CGAGAACCTGCTGCTTAAATCCGGCAACAAGCACGCATCGTCAGATGAATGATGAGCAGCTTAAGGAAGCAGGAGTGCCG
GCAGAGCTTATCAGAATAAGCTTAGGTCTTGAGGACAAGGTGGATTTGATTGCAGATATCTCAAACGCACTCGACGCTAT
AAAATAA

Upstream 100 bases:

>100_bases
ATTACTCTCACACTTCCACGATTTGAAGAATTTGTCAAAACGCGACAAATGTAATATACTATATCCAACTGAAACATTAA
CATAATAAAGGAGAAGCACA

Downstream 100 bases:

>100_bases
AATATATATTCATAAAATACACAAAAAGAACAAAATTAGTAAAAACAAAACTTTACGAAATGACTTACTAATGATATAGT
AGTGATAGGTATGAAAGAGT

Product: putative cysteine biosynthesis related protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKEYSIGTKCVQAGYAAGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEAEGYFYSRLQNPTCDTVARKIAAMEGGTAAML
TSSGQAANFYAMFNICECGDHIVASSSIYGGTFNLIAVTMAKMGITATFVSPDATEEELNAAFRPNTKLMFGETIANPAL
TVLDIELFAKVAHEHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGATLGGAIVDSGKFDWMAHADKYPGLCT
PDDSYHGITYAEKFGKEGAFITKCTAQLMRDFGSMQSPQNAFMLNLGLESLHVRMPKHVENGQAVAEFLENHPKVAYVNY
SGLPSNKYYERAQKYLPNGGCGVVSFGLKGGREAASAFMKALRLGAIETHVADARTCCLNPATSTHRQMNDEQLKEAGVP
AELIRISLGLEDKVDLIADISNALDAIK

Sequences:

>Translated_428_residues
MKEYSIGTKCVQAGYAAGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEAEGYFYSRLQNPTCDTVARKIAAMEGGTAAML
TSSGQAANFYAMFNICECGDHIVASSSIYGGTFNLIAVTMAKMGITATFVSPDATEEELNAAFRPNTKLMFGETIANPAL
TVLDIELFAKVAHEHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGATLGGAIVDSGKFDWMAHADKYPGLCT
PDDSYHGITYAEKFGKEGAFITKCTAQLMRDFGSMQSPQNAFMLNLGLESLHVRMPKHVENGQAVAEFLENHPKVAYVNY
SGLPSNKYYERAQKYLPNGGCGVVSFGLKGGREAASAFMKALRLGAIETHVADARTCCLNPATSTHRQMNDEQLKEAGVP
AELIRISLGLEDKVDLIADISNALDAIK
>Mature_428_residues
MKEYSIGTKCVQAGYAAGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEAEGYFYSRLQNPTCDTVARKIAAMEGGTAAML
TSSGQAANFYAMFNICECGDHIVASSSIYGGTFNLIAVTMAKMGITATFVSPDATEEELNAAFRPNTKLMFGETIANPAL
TVLDIELFAKVAHEHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGATLGGAIVDSGKFDWMAHADKYPGLCT
PDDSYHGITYAEKFGKEGAFITKCTAQLMRDFGSMQSPQNAFMLNLGLESLHVRMPKHVENGQAVAEFLENHPKVAYVNY
SGLPSNKYYERAQKYLPNGGCGVVSFGLKGGREAASAFMKALRLGAIETHVADARTCCLNPATSTHRQMNDEQLKEAGVP
AELIRISLGLEDKVDLIADISNALDAIK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=386, Percent_Identity=33.160621761658, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=31.7617866004963, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI25453487, Length=402, Percent_Identity=29.1044776119403, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=31.3157894736842, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=24.5327102803738, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17560056, Length=411, Percent_Identity=33.5766423357664, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=411, Percent_Identity=32.6034063260341, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=29.6116504854369, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=416, Percent_Identity=41.1057692307692, Blast_Score=303, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6319307, Length=424, Percent_Identity=30.8962264150943, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=424, Percent_Identity=25.7075471698113, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=26.9461077844311, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=32.7830188679245, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46327; Mature: 46327

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYSIGTKCVQAGYAAGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEAEGYFYSRLQNP
CCCCCCCHHHHHHCCCCCCCCCCEEEEEECCEEECCCCHHHHHHEECCCCCEEHHHCCCC
TCDTVARKIAAMEGGTAAMLTSSGQAANFYAMFNICECGDHIVASSSIYGGTFNLIAVTM
CHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEHHHCCCCEEECCCCCCCHHHHHHHHH
AKMGITATFVSPDATEEELNAAFRPNTKLMFGETIANPALTVLDIELFAKVAHEHGVPLI
HHHCEEEEEECCCCCHHHHHHHCCCCCEEEECHHHCCCCEEEEHHHHHHHHHHHCCCEEE
VDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGATLGGAIVDSGKFDWMAHADKYPGLCT
ECCCCCCCCCCEEECCCCHHHCCCCHHEECCCCCEECCEEECCCCCCEEECCCCCCCCCC
PDDSYHGITYAEKFGKEGAFITKCTAQLMRDFGSMQSPQNAFMLNLGLESLHVRMPKHVE
CCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHEECCCCCC
NGQAVAEFLENHPKVAYVNYSGLPSNKYYERAQKYLPNGGCGVVSFGLKGGREAASAFMK
CHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHH
ALRLGAIETHVADARTCCLNPATSTHRQMNDEQLKEAGVPAELIRISLGLEDKVDLIADI
HHHHCHHHHHHHCCHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHEEECCCCCHHHHHHHH
SNALDAIK
HHHHHHCC
>Mature Secondary Structure
MKEYSIGTKCVQAGYAAGNGEPRQIPIIQSTTFKYDTSEDMGKLFDLEAEGYFYSRLQNP
CCCCCCCHHHHHHCCCCCCCCCCEEEEEECCEEECCCCHHHHHHEECCCCCEEHHHCCCC
TCDTVARKIAAMEGGTAAMLTSSGQAANFYAMFNICECGDHIVASSSIYGGTFNLIAVTM
CHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEHHHCCCCEEECCCCCCCHHHHHHHHH
AKMGITATFVSPDATEEELNAAFRPNTKLMFGETIANPALTVLDIELFAKVAHEHGVPLI
HHHCEEEEEECCCCCHHHHHHHCCCCCEEEECHHHCCCCEEEEHHHHHHHHHHHCCCEEE
VDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGATLGGAIVDSGKFDWMAHADKYPGLCT
ECCCCCCCCCCEEECCCCHHHCCCCHHEECCCCCEECCEEECCCCCCEEECCCCCCCCCC
PDDSYHGITYAEKFGKEGAFITKCTAQLMRDFGSMQSPQNAFMLNLGLESLHVRMPKHVE
CCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHEECCCCCC
NGQAVAEFLENHPKVAYVNYSGLPSNKYYERAQKYLPNGGCGVVSFGLKGGREAASAFMK
CHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHH
ALRLGAIETHVADARTCCLNPATSTHRQMNDEQLKEAGVPAELIRISLGLEDKVDLIADI
HHHHCHHHHHHHCCHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHEEECCCCCHHHHHHHH
SNALDAIK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]