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The map label for this gene is nirB [C]

Identifier: 238923284

GI number: 238923284

Start: 801833

End: 802702

Strand: Direct

Name: nirB [C]

Synonym: EUBREC_0895

Alternate gene names: 238923284

Gene position: 801833-802702 (Clockwise)

Preceding gene: 238923283

Following gene: 238923285

Centisome position: 23.24

GC content: 46.9

Gene sequence:

>870_bases
ATGGCGGAGAAAGTTGATTACGCGGCCCTAAAAAAGGGCGGTTTCATGAGACAGAAGCAAAAGGGCTGTTTCTCGCTTAG
AATTGCGGTTGTAGGAGGAAATCTTACAGCAGAAAATATCAAAACAGTAGCAGAGGTTGCTGAGAAATACGGACACGGAT
ATGTTCATATGACATCGCGTCAGGGTATTGAGATTCCTTTTATCAAGGTTGAGGATATCAATGTGGTAAAAGAGGAACTG
GCAAAGGGCGGTGTAGGAACAGGTGTCTGCGGACCGAGAGTACGTACTGTCACAGCGTGTCAGGGCTCAGAAATCTGTCC
TAGCGGATGTATCGATACATACACTCTGGCTAAGGAACTTGATGAAAGATACTTCGGAAGAGAGCTTCCTCACAAGTTCA
AGTTTGGTGTCACAGGCTGTCAGAACAACTGCTTAAAGGCTGAGGAAAACGATGTCGGAATCAAAGGCGGCATGACAGTA
GAGTGCAGCCATGACGATTGTATCCAGTGTGGTGTATGTGTGAAGGCATGCCGCGAGGGTGCACTTTCGATGGAGGATGG
CAGGATTGTCATCGACAGAGACAAATGTAACAACTGTGCGCGCTGCGTAAAGAGCTGTCCGACAGATGCATGGAAGGGAA
CTCCGGGATATATCGTATCGTTTGGAGGACTTTTCGGAAACCACATCTATAAAGGAGAGCAGCTAGTTCCGATTATCCGC
GACAAGGAAACTCTTTTCAGAGTGACAGATGCAGCAATTAGTTTCTTCGAGAAGCATGCAAACGGTGGTGAGCGTTTCAG
ACTTACTCTTCAGAGAGTGGGCGAGGAAGAGTTCAAAAAAGAGATTCAGGCAGCATACGAGGGTAAATAA

Upstream 100 bases:

>100_bases
CATCGTAATAAGGTATCAATGTGTATGCAAATGAGAGCGTGCATATGCATTTACATATAAAACAAAACATTAACATATTT
ACACAGTAAGGAGATTAAAA

Downstream 100 bases:

>100_bases
TCCCCATGTATAGCATGAAATATCCGGCATGCATAAACAACAACGGTTACGGCAAAAATATAATAAATATGAGTATAGGA
AGGGAGATGCAAAATGGCAG

Product: sulfite reductase, beta subunit

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAEKVDYAALKKGGFMRQKQKGCFSLRIAVVGGNLTAENIKTVAEVAEKYGHGYVHMTSRQGIEIPFIKVEDINVVKEEL
AKGGVGTGVCGPRVRTVTACQGSEICPSGCIDTYTLAKELDERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGGMTV
ECSHDDCIQCGVCVKACREGALSMEDGRIVIDRDKCNNCARCVKSCPTDAWKGTPGYIVSFGGLFGNHIYKGEQLVPIIR
DKETLFRVTDAAISFFEKHANGGERFRLTLQRVGEEEFKKEIQAAYEGK

Sequences:

>Translated_289_residues
MAEKVDYAALKKGGFMRQKQKGCFSLRIAVVGGNLTAENIKTVAEVAEKYGHGYVHMTSRQGIEIPFIKVEDINVVKEEL
AKGGVGTGVCGPRVRTVTACQGSEICPSGCIDTYTLAKELDERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGGMTV
ECSHDDCIQCGVCVKACREGALSMEDGRIVIDRDKCNNCARCVKSCPTDAWKGTPGYIVSFGGLFGNHIYKGEQLVPIIR
DKETLFRVTDAAISFFEKHANGGERFRLTLQRVGEEEFKKEIQAAYEGK
>Mature_288_residues
AEKVDYAALKKGGFMRQKQKGCFSLRIAVVGGNLTAENIKTVAEVAEKYGHGYVHMTSRQGIEIPFIKVEDINVVKEELA
KGGVGTGVCGPRVRTVTACQGSEICPSGCIDTYTLAKELDERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGGMTVE
CSHDDCIQCGVCVKACREGALSMEDGRIVIDRDKCNNCARCVKSCPTDAWKGTPGYIVSFGGLFGNHIYKGEQLVPIIRD
KETLFRVTDAAISFFEKHANGGERFRLTLQRVGEEEFKKEIQAAYEGK

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789765, Length=142, Percent_Identity=33.0985915492958, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR007516
- InterPro:   IPR007525
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF00037 Fer4; PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.12.98.1

Molecular weight: Translated: 31745; Mature: 31614

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00365 NIR_SIR ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKVDYAALKKGGFMRQKQKGCFSLRIAVVGGNLTAENIKTVAEVAEKYGHGYVHMTSR
CCCCCCHHHHHCCCCHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECC
QGIEIPFIKVEDINVVKEELAKGGVGTGVCGPRVRTVTACQGSEICPSGCIDTYTLAKEL
CCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
DERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGGMTVECSHDDCIQCGVCVKACREG
HHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
ALSMEDGRIVIDRDKCNNCARCVKSCPTDAWKGTPGYIVSFGGLFGNHIYKGEQLVPIIR
CCEECCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHCCCCCCEEEEEC
DKETLFRVTDAAISFFEKHANGGERFRLTLQRVGEEEFKKEIQAAYEGK
CCHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKVDYAALKKGGFMRQKQKGCFSLRIAVVGGNLTAENIKTVAEVAEKYGHGYVHMTSR
CCCCCHHHHHCCCCHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECC
QGIEIPFIKVEDINVVKEELAKGGVGTGVCGPRVRTVTACQGSEICPSGCIDTYTLAKEL
CCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
DERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGGMTVECSHDDCIQCGVCVKACREG
HHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
ALSMEDGRIVIDRDKCNNCARCVKSCPTDAWKGTPGYIVSFGGLFGNHIYKGEQLVPIIR
CCEECCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHCCCCCCEEEEEC
DKETLFRVTDAAISFFEKHANGGERFRLTLQRVGEEEFKKEIQAAYEGK
CCHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]