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The map label for this gene is metZ [H]

Identifier: 238923283

GI number: 238923283

Start: 800498

End: 801730

Strand: Direct

Name: metZ [H]

Synonym: EUBREC_0894

Alternate gene names: 238923283

Gene position: 800498-801730 (Clockwise)

Preceding gene: 238923282

Following gene: 238923284

Centisome position: 23.2

GC content: 43.55

Gene sequence:

>1233_bases
ATGAGATTTAATACAAGCTTACTTCATGCCGGCGTGACAAAAGAGGCAAATGGCTCTACACTGCCACCGGTATATCAGAC
AAGCGCCTTCGAGCAGGAGCGCGCGGCAGATCTGGAAAAAATATTTGAAAACAAAGCTCCGGGCTACTGTTACACAAGAG
TTGCCAATCCTACGATTACAGCTTTTGAGAACAGAATAACAAAGCTCGAGGGAGGAATAGCATCGGTGGCATGCGCATCA
GGCATGGCAGCACTGATGAATACCTTCTTAAATATACTAAGCAGTGGAGATGAGCTCATCTCAAGCGCCAGCCTCTACGG
TGGCACAATAGATTTGTTCCATGATCTTGAGGCCTTCGGCATCACAACCAGATATGTCGAGAACAACAACTGGCAGCAGA
TAGAGGACAGCATCAATGAGCATACCAGACTGATTTTTGCAGAGACTATCGGTAATCCCTGTCTGGATGTCACAGATATT
GAGAAGCTTGCAGAGATAGCACACGCACATGATATACCGCTTGTAGTTGACAATACCACATCGACAGCTTACCTGATACA
GGTTTTAAAGCATGGAGCTGATATCGTGGTCAATTCATCTTCCAAATATATCAATGGAAGCAGCAATGCAATCAGTGGTA
TTCTGACAGACTCAGGTAAGTTCAAATGGGACAAGAACAGATATCCGGGGTTTGCAGATTATGTGAAGTATGGACCTATG
GCATTTGTCGCAAAGCTGAGAAACAGTCTTTTCAGAAATATGGGTGCCTGTTTAGCTCCGGTGAATGCATACTTAAATAT
TATAGGACTTGAGACACTCGGTCTTAGAATGGAAAGAGAATGCTCTAATGCACTCGGGCTTGCGTCGTGGATAGAAAACA
ATTATCCTGACATAAAGGTTAATTATCCGGGGCTTTGTAGCAGCAAATGGCATGAAATAGCAAAAAAACAGCTCACAAAC
GGCTATGGAGCAATCCTTACAATCAGGGTTGGCAGCAAGGAAAAGGCATTTAAATTTATAGACAGCCTGACGATTCCATA
CACACTCAGCAATATCGGAGATACAAAGACACTTGTCATTCATCCGGGCTCCACAATCAGTCTTCACAGCACAGATAAGC
AGAAGGAGGATGCAGGAGTATTTGAGGATCTTATAAGAGTCAGTGTGGGTATCGAGGATATAGATGACCTCAAGGAGGAT
TTTGCACAGGCTTTTTTAAATATAGCTGATTAA

Upstream 100 bases:

>100_bases
ACTGATAGCATTGACTTTCAACACACACGTTTGTATAATAATCTCATCAATTCCTAGTAACACAATAGACTATGTAAGAA
TTAACGAGAAGAAGAAAACA

Downstream 100 bases:

>100_bases
GCCATCGTAATAAGGTATCAATGTGTATGCAAATGAGAGCGTGCATATGCATTTACATATAAAACAAAACATTAACATAT
TTACACAGTAAGGAGATTAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MRFNTSLLHAGVTKEANGSTLPPVYQTSAFEQERAADLEKIFENKAPGYCYTRVANPTITAFENRITKLEGGIASVACAS
GMAALMNTFLNILSSGDELISSASLYGGTIDLFHDLEAFGITTRYVENNNWQQIEDSINEHTRLIFAETIGNPCLDVTDI
EKLAEIAHAHDIPLVVDNTTSTAYLIQVLKHGADIVVNSSSKYINGSSNAISGILTDSGKFKWDKNRYPGFADYVKYGPM
AFVAKLRNSLFRNMGACLAPVNAYLNIIGLETLGLRMERECSNALGLASWIENNYPDIKVNYPGLCSSKWHEIAKKQLTN
GYGAILTIRVGSKEKAFKFIDSLTIPYTLSNIGDTKTLVIHPGSTISLHSTDKQKEDAGVFEDLIRVSVGIEDIDDLKED
FAQAFLNIAD

Sequences:

>Translated_410_residues
MRFNTSLLHAGVTKEANGSTLPPVYQTSAFEQERAADLEKIFENKAPGYCYTRVANPTITAFENRITKLEGGIASVACAS
GMAALMNTFLNILSSGDELISSASLYGGTIDLFHDLEAFGITTRYVENNNWQQIEDSINEHTRLIFAETIGNPCLDVTDI
EKLAEIAHAHDIPLVVDNTTSTAYLIQVLKHGADIVVNSSSKYINGSSNAISGILTDSGKFKWDKNRYPGFADYVKYGPM
AFVAKLRNSLFRNMGACLAPVNAYLNIIGLETLGLRMERECSNALGLASWIENNYPDIKVNYPGLCSSKWHEIAKKQLTN
GYGAILTIRVGSKEKAFKFIDSLTIPYTLSNIGDTKTLVIHPGSTISLHSTDKQKEDAGVFEDLIRVSVGIEDIDDLKED
FAQAFLNIAD
>Mature_410_residues
MRFNTSLLHAGVTKEANGSTLPPVYQTSAFEQERAADLEKIFENKAPGYCYTRVANPTITAFENRITKLEGGIASVACAS
GMAALMNTFLNILSSGDELISSASLYGGTIDLFHDLEAFGITTRYVENNNWQQIEDSINEHTRLIFAETIGNPCLDVTDI
EKLAEIAHAHDIPLVVDNTTSTAYLIQVLKHGADIVVNSSSKYINGSSNAISGILTDSGKFKWDKNRYPGFADYVKYGPM
AFVAKLRNSLFRNMGACLAPVNAYLNIIGLETLGLRMERECSNALGLASWIENNYPDIKVNYPGLCSSKWHEIAKKQLTN
GYGAILTIRVGSKEKAFKFIDSLTIPYTLSNIGDTKTLVIHPGSTISLHSTDKQKEDAGVFEDLIRVSVGIEDIDDLKED
FAQAFLNIAD

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=393, Percent_Identity=31.0432569974555, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI299473759, Length=392, Percent_Identity=27.5510204081633, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI25453487, Length=392, Percent_Identity=25.765306122449, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1790375, Length=403, Percent_Identity=27.5434243176179, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=23.5294117647059, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=388, Percent_Identity=32.2164948453608, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17538055, Length=398, Percent_Identity=27.8894472361809, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=398, Percent_Identity=27.8894472361809, Blast_Score=148, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=40.7407407407407, Blast_Score=282, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6319307, Length=412, Percent_Identity=32.2815533980583, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=433, Percent_Identity=26.5588914549654, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=299, Percent_Identity=28.7625418060201, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=424, Percent_Identity=25.2358490566038, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322970, Length=276, Percent_Identity=26.4492753623188, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922554, Length=410, Percent_Identity=30.2439024390244, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 44975; Mature: 44975

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNTSLLHAGVTKEANGSTLPPVYQTSAFEQERAADLEKIFENKAPGYCYTRVANPTIT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
AFENRITKLEGGIASVACASGMAALMNTFLNILSSGDELISSASLYGGTIDLFHDLEAFG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHC
ITTRYVENNNWQQIEDSINEHTRLIFAETIGNPCLDVTDIEKLAEIAHAHDIPLVVDNTT
CEEEEEECCCHHHHHHHHHHHHEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
STAYLIQVLKHGADIVVNSSSKYINGSSNAISGILTDSGKFKWDKNRYPGFADYVKYGPM
HHHHHHHHHHCCCCEEEECCCCEECCCCCCEEEEEECCCCCEECCCCCCCHHHHHHHCHH
AFVAKLRNSLFRNMGACLAPVNAYLNIIGLETLGLRMERECSNALGLASWIENNYPDIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEE
NYPGLCSSKWHEIAKKQLTNGYGAILTIRVGSKEKAFKFIDSLTIPYTLSNIGDTKTLVI
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCEEECCCCCCEEEEE
HPGSTISLHSTDKQKEDAGVFEDLIRVSVGIEDIDDLKEDFAQAFLNIAD
ECCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFNTSLLHAGVTKEANGSTLPPVYQTSAFEQERAADLEKIFENKAPGYCYTRVANPTIT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
AFENRITKLEGGIASVACASGMAALMNTFLNILSSGDELISSASLYGGTIDLFHDLEAFG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHC
ITTRYVENNNWQQIEDSINEHTRLIFAETIGNPCLDVTDIEKLAEIAHAHDIPLVVDNTT
CEEEEEECCCHHHHHHHHHHHHEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
STAYLIQVLKHGADIVVNSSSKYINGSSNAISGILTDSGKFKWDKNRYPGFADYVKYGPM
HHHHHHHHHHCCCCEEEECCCCEECCCCCCEEEEEECCCCCEECCCCCCCHHHHHHHCHH
AFVAKLRNSLFRNMGACLAPVNAYLNIIGLETLGLRMERECSNALGLASWIENNYPDIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEE
NYPGLCSSKWHEIAKKQLTNGYGAILTIRVGSKEKAFKFIDSLTIPYTLSNIGDTKTLVI
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCEEECCCCCCEEEEE
HPGSTISLHSTDKQKEDAGVFEDLIRVSVGIEDIDDLKEDFAQAFLNIAD
ECCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]