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The map label for this gene is lacA [C]

Identifier: 238923161

GI number: 238923161

Start: 693894

End: 694475

Strand: Direct

Name: lacA [C]

Synonym: EUBREC_0754

Alternate gene names: 238923161

Gene position: 693894-694475 (Clockwise)

Preceding gene: 238923160

Following gene: 238923162

Centisome position: 20.11

GC content: 34.02

Gene sequence:

>582_bases
ATGAGTAGAAAAATTAATAATATTATATTCAGACAGCGGGTATGTTGTTATAAAGATACATATTTGTGTATTGATGATAT
TGATGGTCTGAAAATTCATGGAAAATTAAAGTTGAATTGGAATCCAATTAGTAAGTCAATTCGGACGGGGCAAGCTACTT
TTTTTAGAATGGACAACCATTCTCAGTTGAGAGTTGGTGGAAATTTTGATGTTTTCTATGGTAACGATGTACACATATTC
AGCGGTGGAAAATTGGAATTAGGGAGTGGGTTTATTAATTCATACGGTAAAATTGAGTGTCATAAACATATAAAAATAGG
AAAAAACTGTGCAGTAGGACCGTATACTATCATTCTTGATTCCGATGGGCATCAAATATTTGGTAAACGTAATACAGATG
AAGTGATTATTGGAAATAACGTATGGATTGGTGCTAGAGTTACTATATTAAAAGGTGTGCGTATAGGAGATGGTGCAGTT
ATTGCAGCAGGAACTATTGTGAATAAAGATGTACCAGCAAGAGCTTTGGCTGCTGGTAATCCAATGCATTTGATATGTGA
TAATGTTGAGTGGACTAATTAG

Upstream 100 bases:

>100_bases
TTTAGATTGCGCCCTAATTCTCTGAACCCCGAATATTTTCGAATTGATTTTTCGAAAATATTGATAAGTCTTGCATGTGT
GTTGTCAAAGGTAGAGTGGC

Downstream 100 bases:

>100_bases
TTAAAAATATTCGAGGGGATTGATTGGTAATAGATTGTTGTATTAGTAAATGACAAATCGAGGTATAAGTATGAATGATT
ATATTATTCTTGTAAATGAA

Product: galactoside acetyltransferase (LacA)

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSRKINNIIFRQRVCCYKDTYLCIDDIDGLKIHGKLKLNWNPISKSIRTGQATFFRMDNHSQLRVGGNFDVFYGNDVHIF
SGGKLELGSGFINSYGKIECHKHIKIGKNCAVGPYTIILDSDGHQIFGKRNTDEVIIGNNVWIGARVTILKGVRIGDGAV
IAAGTIVNKDVPARALAAGNPMHLICDNVEWTN

Sequences:

>Translated_193_residues
MSRKINNIIFRQRVCCYKDTYLCIDDIDGLKIHGKLKLNWNPISKSIRTGQATFFRMDNHSQLRVGGNFDVFYGNDVHIF
SGGKLELGSGFINSYGKIECHKHIKIGKNCAVGPYTIILDSDGHQIFGKRNTDEVIIGNNVWIGARVTILKGVRIGDGAV
IAAGTIVNKDVPARALAAGNPMHLICDNVEWTN
>Mature_192_residues
SRKINNIIFRQRVCCYKDTYLCIDDIDGLKIHGKLKLNWNPISKSIRTGQATFFRMDNHSQLRVGGNFDVFYGNDVHIFS
GGKLELGSGFINSYGKIECHKHIKIGKNCAVGPYTIILDSDGHQIFGKRNTDEVIIGNNVWIGARVTILKGVRIGDGAVI
AAGTIVNKDVPARALAAGNPMHLICDNVEWTN

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=119, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786664, Length=87, Percent_Identity=42.5287356321839, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21359; Mature: 21228

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKINNIIFRQRVCCYKDTYLCIDDIDGLKIHGKLKLNWNPISKSIRTGQATFFRMDNH
CCCCHHHHHHHHEEEEECCCEEEEECCCCEEEEEEEEEEECHHCCCEECCCEEEEEECCC
SQLRVGGNFDVFYGNDVHIFSGGKLELGSGFINSYGKIECHKHIKIGKNCAVGPYTIILD
CEEEECCCEEEEECCEEEEEECCEEEECCCCCCCCCEEEEEEEEEECCCCCCCCEEEEEE
SDGHQIFGKRNTDEVIIGNNVWIGARVTILKGVRIGDGAVIAAGTIVNKDVPARALAAGN
CCCCEEECCCCCCCEEECCCEEEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHCCC
PMHLICDNVEWTN
CEEEEECCCEECC
>Mature Secondary Structure 
SRKINNIIFRQRVCCYKDTYLCIDDIDGLKIHGKLKLNWNPISKSIRTGQATFFRMDNH
CCCHHHHHHHHEEEEECCCEEEEECCCCEEEEEEEEEEECHHCCCEECCCEEEEEECCC
SQLRVGGNFDVFYGNDVHIFSGGKLELGSGFINSYGKIECHKHIKIGKNCAVGPYTIILD
CEEEECCCEEEEECCEEEEEECCEEEECCCCCCCCCEEEEEEEEEECCCCCCCCEEEEEE
SDGHQIFGKRNTDEVIIGNNVWIGARVTILKGVRIGDGAVIAAGTIVNKDVPARALAAGN
CCCCEEECCCCCCCEEECCCEEEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHCCC
PMHLICDNVEWTN
CEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]