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The map label for this gene is dagK [H]

Identifier: 238923107

GI number: 238923107

Start: 642183

End: 643067

Strand: Direct

Name: dagK [H]

Synonym: EUBREC_0699

Alternate gene names: 238923107

Gene position: 642183-643067 (Clockwise)

Preceding gene: 238923106

Following gene: 238923108

Centisome position: 18.62

GC content: 47.34

Gene sequence:

>885_bases
ATGAAGAAAAAGCTTTTATTTGTATTTAATCCCAAATCAGGCAAGGGACTCATAAAGGAGCACCTGGTAAATATCGTGGA
CATCATGACAAAGGCCGGATACAAAATCACCATTTATCCTACACAGTGTCAGGGGGATGCCATAAAAAAGGTTAGGAAAA
ATGCTGAGAAGTATGACCTTGTAGTGTGCAGCGGCGGAGACGGCACACTCGACGAGGTAGTGACCGGCATGGAGCAAAGC
GAGGTAAATGTACCGATAGGATACATCCCTGCGGGCAGCACCAATGACTTTGCAAACTCTCTCGGCATACCCAAAAATAT
GGAGGAGGCCGCAAGAGTTGCCGTAAACGGTACTCCATTCCCGTGTGATGTGGGAGGCTTCAACGGAGATACCTTTGTAT
ATGTCGCAGCCTTTGGCCTTTTTACAGAGGTATCCTACCAGACCTCGCAGCAGATGAAAAATATACTGGGACATGCCGCA
TATATTTTAGAGGGTGCAAAGCACCTGATGGATATCACCTCGTACAGGATGAAGGTGAGCCATGACGGTGAGATAATAGA
GGATGAGTTTATATATGGAATGGTGACCAACTCCCTTTCGGTAGGAGGCTTCAAGGGCATATCAGGTCCTGACGTACTGC
TTGATGACGGCCTTTTTGAGGTGACACTCATCAAGAATCCAAAAAACCCAATCGAGCTCAACAAGATACTTGCGGGACTG
GCAAACCGTGAAAATGACAATGAGCTCATCTACTCCTTCAAGACCAGAGAGCTGCATGTGGAGAGTGAGGAGGAGGTACC
GTGGACACTCGACGGAGAGTTTGGCGGCAGCCACACGGATCTGACCATCACTAATCTGAACAAACAGATTACCATTATGT
GCTGA

Upstream 100 bases:

>100_bases
GCTAAAGGAGCTCGGAGGATATCTGGATCCGCCAATGGGTAAATCGGGAGTCAACCACAGGCTTAGAAAATTATCGGCGA
TTGCGGAGGAACTGAGATAG

Downstream 100 bases:

>100_bases
TAAATACAAAATCGGGCATAAAATAGTAATAGTACACAATTTTTTGTCTGATTGTGTTAAAAATTTAACTTTATTTTTAT
AAGGTTATGTAATATAATAA

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKKKLLFVFNPKSGKGLIKEHLVNIVDIMTKAGYKITIYPTQCQGDAIKKVRKNAEKYDLVVCSGGDGTLDEVVTGMEQS
EVNVPIGYIPAGSTNDFANSLGIPKNMEEAARVAVNGTPFPCDVGGFNGDTFVYVAAFGLFTEVSYQTSQQMKNILGHAA
YILEGAKHLMDITSYRMKVSHDGEIIEDEFIYGMVTNSLSVGGFKGISGPDVLLDDGLFEVTLIKNPKNPIELNKILAGL
ANRENDNELIYSFKTRELHVESEEEVPWTLDGEFGGSHTDLTITNLNKQITIMC

Sequences:

>Translated_294_residues
MKKKLLFVFNPKSGKGLIKEHLVNIVDIMTKAGYKITIYPTQCQGDAIKKVRKNAEKYDLVVCSGGDGTLDEVVTGMEQS
EVNVPIGYIPAGSTNDFANSLGIPKNMEEAARVAVNGTPFPCDVGGFNGDTFVYVAAFGLFTEVSYQTSQQMKNILGHAA
YILEGAKHLMDITSYRMKVSHDGEIIEDEFIYGMVTNSLSVGGFKGISGPDVLLDDGLFEVTLIKNPKNPIELNKILAGL
ANRENDNELIYSFKTRELHVESEEEVPWTLDGEFGGSHTDLTITNLNKQITIMC
>Mature_294_residues
MKKKLLFVFNPKSGKGLIKEHLVNIVDIMTKAGYKITIYPTQCQGDAIKKVRKNAEKYDLVVCSGGDGTLDEVVTGMEQS
EVNVPIGYIPAGSTNDFANSLGIPKNMEEAARVAVNGTPFPCDVGGFNGDTFVYVAAFGLFTEVSYQTSQQMKNILGHAA
YILEGAKHLMDITSYRMKVSHDGEIIEDEFIYGMVTNSLSVGGFKGISGPDVLLDDGLFEVTLIKNPKNPIELNKILAGL
ANRENDNELIYSFKTRELHVESEEEVPWTLDGEFGGSHTDLTITNLNKQITIMC

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=273, Percent_Identity=26.7399267399267, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32243; Mature: 32243

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLLFVFNPKSGKGLIKEHLVNIVDIMTKAGYKITIYPTQCQGDAIKKVRKNAEKYDL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCEEE
VVCSGGDGTLDEVVTGMEQSEVNVPIGYIPAGSTNDFANSLGIPKNMEEAARVAVNGTPF
EEEECCCCCHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHEEEECCCCC
PCDVGGFNGDTFVYVAAFGLFTEVSYQTSQQMKNILGHAAYILEGAKHLMDITSYRMKVS
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
HDGEIIEDEFIYGMVTNSLSVGGFKGISGPDVLLDDGLFEVTLIKNPKNPIELNKILAGL
CCCCEECCCEEEEEEECCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCHHHHHHHHHH
ANRENDNELIYSFKTRELHVESEEEVPWTLDGEFGGSHTDLTITNLNKQITIMC
HCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEEC
>Mature Secondary Structure
MKKKLLFVFNPKSGKGLIKEHLVNIVDIMTKAGYKITIYPTQCQGDAIKKVRKNAEKYDL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCEEE
VVCSGGDGTLDEVVTGMEQSEVNVPIGYIPAGSTNDFANSLGIPKNMEEAARVAVNGTPF
EEEECCCCCHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHEEEECCCCC
PCDVGGFNGDTFVYVAAFGLFTEVSYQTSQQMKNILGHAAYILEGAKHLMDITSYRMKVS
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
HDGEIIEDEFIYGMVTNSLSVGGFKGISGPDVLLDDGLFEVTLIKNPKNPIELNKILAGL
CCCCEECCCEEEEEEECCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCHHHHHHHHHH
ANRENDNELIYSFKTRELHVESEEEVPWTLDGEFGGSHTDLTITNLNKQITIMC
HCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]