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The map label for this gene is cca [H]

Identifier: 238922936

GI number: 238922936

Start: 484396

End: 485736

Strand: Direct

Name: cca [H]

Synonym: EUBREC_0524

Alternate gene names: 238922936

Gene position: 484396-485736 (Clockwise)

Preceding gene: 238922935

Following gene: 238922937

Centisome position: 14.04

GC content: 48.47

Gene sequence:

>1341_bases
ATGCGCATAGATTTACCGCAGAATGTCAACTTCATAATAGACACGCTCTACGAGCATGGCTTTGAGGCATATGCGGTAGG
TGGATGCGTGCGTGACAGTCTCCTGGGCCGGGTGCCACAGGACTGGGATATCACCACATCTGCAAAGCCTGCGCAGGTCA
AAGCGATATTTGACCATACGATAGACACAGGTATCCAGCATGGCACAGTCACGGTCATGCTGGAGCATGTGGGCTATGAG
GTCACCACCTACAGGATAGACGGAGAGTACGAGGATGCACGCCATCCAAAGGAGGTTACCTTTACCTCCAATCTGAAGCT
TGATCTGGAGAGGCGCGACTTCACAATCAATGCCATGGCATATAATCATAGGGCAGGGCTTGTCGATGAATTTGACGGCA
TAGCAGATTTAAAAGCACATGTGATCAGATGTGTGGGCTGTGCACACGACAGGTTCAGTGAGGATGCACTCAGGATGTTT
CGTGCGGTGCGATTTGCAGCCCAGCTTGGCTTTGATATAGAGGCACAGACTAAAGCAGCCATAAAAGAGCTTGCCCCTGC
CTTGTCAAAGGTCAGCGCAGAGCGAATCCAGGTGGAGCTTGTGAAGCTTCTGACATCAGACCATCCGCAGATGATGAGAG
ATCTGTATGAGCTTGGACTCACAGCGGTATTCATGCCGGAGTTTGATGTGATGATGCAGACACCTCAGCACAATAAGCAC
CATATGTACAGTGTCGGCGAGCATACGCTTCATACCTTGGAGCATGTGCGAGCGGACAAAATCCTAAGGCTTACTATGCT
GCTTCATGATGTGGCAAAGCCTGTGTGCATTACGACAGATGAGGAAGGCCAGAATCATTTCAAGAAGCATCCTGTTGTGG
GAGCTGATATGACAAGAAAGATACTCAGGCGCCTTAAGTTTGATAACAGGACTACAGACTGTTGCTGTAAGCTCGTAAAA
GAGCATGATGACCGTCCGGCCATCACAGAGCGCAATGTAAGGCGTGCCATAAGCCGCATAGGAACAGAGCTTTTTCCGCT
GTTGTTTGAGGTGAAAAGAGCCGATACACTCGGACAGAGCATGTATAAGCGCGCAGAAAAGCTTGAATACATCGCAGAAT
ATGAGAGTGTATACAGGAAAATCCTCGCAGACCACCAGTGTGTATCAAAAAAGGAAATGAAAATAAATGGCAGCGACCTG
ATTAAAATGGGAGTAGAGCCGGGACCGAAGCTTGGCGATATCCTGGACAGGCTTTATGAGCAGGTGCTTGATGACCCATC
GCTCAATGAAGCACAAAAACTTAAAGAGCTTGCGAATAAAATAATAACCTCTCTCATATGA

Upstream 100 bases:

>100_bases
ACCAGACTAAGGAGTTATGAGAGCATGTGTCACAGTTGTATGCTGTGGCAATGTGCCCCTCCGGCGAGCGTCATTTAGAA
TAAAAAAGGAGAATCCAACG

Downstream 100 bases:

>100_bases
TTAAAAAGCATCTTAATAGTTATGATGCATTAATTATATGAGGGGGGATTTTTTTGTATAATCAGCCGGAAGTGGCTTTG
GAAAAATATGAGCTTACAGT

Product: tRNA nucleotidyltransferase (CCA-adding enzyme)

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MRIDLPQNVNFIIDTLYEHGFEAYAVGGCVRDSLLGRVPQDWDITTSAKPAQVKAIFDHTIDTGIQHGTVTVMLEHVGYE
VTTYRIDGEYEDARHPKEVTFTSNLKLDLERRDFTINAMAYNHRAGLVDEFDGIADLKAHVIRCVGCAHDRFSEDALRMF
RAVRFAAQLGFDIEAQTKAAIKELAPALSKVSAERIQVELVKLLTSDHPQMMRDLYELGLTAVFMPEFDVMMQTPQHNKH
HMYSVGEHTLHTLEHVRADKILRLTMLLHDVAKPVCITTDEEGQNHFKKHPVVGADMTRKILRRLKFDNRTTDCCCKLVK
EHDDRPAITERNVRRAISRIGTELFPLLFEVKRADTLGQSMYKRAEKLEYIAEYESVYRKILADHQCVSKKEMKINGSDL
IKMGVEPGPKLGDILDRLYEQVLDDPSLNEAQKLKELANKIITSLI

Sequences:

>Translated_446_residues
MRIDLPQNVNFIIDTLYEHGFEAYAVGGCVRDSLLGRVPQDWDITTSAKPAQVKAIFDHTIDTGIQHGTVTVMLEHVGYE
VTTYRIDGEYEDARHPKEVTFTSNLKLDLERRDFTINAMAYNHRAGLVDEFDGIADLKAHVIRCVGCAHDRFSEDALRMF
RAVRFAAQLGFDIEAQTKAAIKELAPALSKVSAERIQVELVKLLTSDHPQMMRDLYELGLTAVFMPEFDVMMQTPQHNKH
HMYSVGEHTLHTLEHVRADKILRLTMLLHDVAKPVCITTDEEGQNHFKKHPVVGADMTRKILRRLKFDNRTTDCCCKLVK
EHDDRPAITERNVRRAISRIGTELFPLLFEVKRADTLGQSMYKRAEKLEYIAEYESVYRKILADHQCVSKKEMKINGSDL
IKMGVEPGPKLGDILDRLYEQVLDDPSLNEAQKLKELANKIITSLI
>Mature_446_residues
MRIDLPQNVNFIIDTLYEHGFEAYAVGGCVRDSLLGRVPQDWDITTSAKPAQVKAIFDHTIDTGIQHGTVTVMLEHVGYE
VTTYRIDGEYEDARHPKEVTFTSNLKLDLERRDFTINAMAYNHRAGLVDEFDGIADLKAHVIRCVGCAHDRFSEDALRMF
RAVRFAAQLGFDIEAQTKAAIKELAPALSKVSAERIQVELVKLLTSDHPQMMRDLYELGLTAVFMPEFDVMMQTPQHNKH
HMYSVGEHTLHTLEHVRADKILRLTMLLHDVAKPVCITTDEEGQNHFKKHPVVGADMTRKILRRLKFDNRTTDCCCKLVK
EHDDRPAITERNVRRAISRIGTELFPLLFEVKRADTLGQSMYKRAEKLEYIAEYESVYRKILADHQCVSKKEMKINGSDL
IKMGVEPGPKLGDILDRLYEQVLDDPSLNEAQKLKELANKIITSLI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=195, Percent_Identity=42.0512820512821, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI87081691, Length=218, Percent_Identity=37.1559633027523, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789436, Length=327, Percent_Identity=30.5810397553517, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71995920, Length=201, Percent_Identity=33.8308457711443, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321016, Length=222, Percent_Identity=31.0810810810811, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281360112, Length=300, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21357337, Length=300, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 50913; Mature: 50913

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDLPQNVNFIIDTLYEHGFEAYAVGGCVRDSLLGRVPQDWDITTSAKPAQVKAIFDHT
CCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
IDTGIQHGTVTVMLEHVGYEVTTYRIDGEYEDARHPKEVTFTSNLKLDLERRDFTINAMA
HHCCCCCCHHEEEEECCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCEEEEEEE
YNHRAGLVDEFDGIADLKAHVIRCVGCAHDRFSEDALRMFRAVRFAAQLGFDIEAQTKAA
ECCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
IKELAPALSKVSAERIQVELVKLLTSDHPQMMRDLYELGLTAVFMPEFDVMMQTPQHNKH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCC
HMYSVGEHTLHTLEHVRADKILRLTMLLHDVAKPVCITTDEEGQNHFKKHPVVGADMTRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHH
ILRRLKFDNRTTDCCCKLVKEHDDRPAITERNVRRAISRIGTELFPLLFEVKRADTLGQS
HHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYKRAEKLEYIAEYESVYRKILADHQCVSKKEMKINGSDLIKMGVEPGPKLGDILDRLYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHEEECCCCCCCHHHHHHHHHH
QVLDDPSLNEAQKLKELANKIITSLI
HHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIDLPQNVNFIIDTLYEHGFEAYAVGGCVRDSLLGRVPQDWDITTSAKPAQVKAIFDHT
CCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
IDTGIQHGTVTVMLEHVGYEVTTYRIDGEYEDARHPKEVTFTSNLKLDLERRDFTINAMA
HHCCCCCCHHEEEEECCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCEEEEEEE
YNHRAGLVDEFDGIADLKAHVIRCVGCAHDRFSEDALRMFRAVRFAAQLGFDIEAQTKAA
ECCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
IKELAPALSKVSAERIQVELVKLLTSDHPQMMRDLYELGLTAVFMPEFDVMMQTPQHNKH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCC
HMYSVGEHTLHTLEHVRADKILRLTMLLHDVAKPVCITTDEEGQNHFKKHPVVGADMTRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHH
ILRRLKFDNRTTDCCCKLVKEHDDRPAITERNVRRAISRIGTELFPLLFEVKRADTLGQS
HHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYKRAEKLEYIAEYESVYRKILADHQCVSKKEMKINGSDLIKMGVEPGPKLGDILDRLYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHEEECCCCCCCHHHHHHHHHH
QVLDDPSLNEAQKLKELANKIITSLI
HHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA