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The map label for this gene is proS

Identifier: 238922935

GI number: 238922935

Start: 482609

End: 484048

Strand: Direct

Name: proS

Synonym: EUBREC_0523

Alternate gene names: 238922935

Gene position: 482609-484048 (Clockwise)

Preceding gene: 238922934

Following gene: 238922936

Centisome position: 13.99

GC content: 47.22

Gene sequence:

>1440_bases
ATGGCTAAGGAGAAAAAATTAGTAGAGGCCATCACTTCCATGGAGGAAGATTTCACACAATGGTATACAGACGTTGTGAA
GAAAGCAGAGTTGATGGATTATTCATCTGTAAAGGGATGTATGATCTTTAAGCCCAATGGTTATGCCATCTGGGAGAATA
TACAGAAAAATCTCGATGCAATGTTCAAAGAGACAGGTGTAGAGAATGTATACATGCCAATGTTCATTCCGGAGAGCCTG
CTCCAGAAGGAAAAGGATCACGTAGAGGGCTTTGCACCTGAGGTCGCATGGGTCACACAGGGCGGACTTGAGACACTTCA
GGAGAGACTTGCAGTAAGACCTACATCAGAGACACTTTTCTGCGAGCTTTTCAGCAAAACAGTACAGTCACACAGAGACC
TTCCAAAGGTATATAACCAGTGGTGCTCAGTAGTGCGCTGGGAGAAGACAACACGTCCATTCCTCCGCTCATCAGAGTTC
TTATGGCAGGAGGGACATACTGTTCATGCAACAGCCGAGGAGGCAGAGGCACGTACAGTACAGATGTTAAACATCTATGC
GGATTTCTGTGAGAAATATCTTGCAATCCCTATGGTAAAGGGAAGAAAGACAGACAAGGAGAAGTTCGCCGGAGCAGAGG
CTACATACACAATAGAGGCACTCATGCATGACGGAAAAGCACTCCAGTCAGGAACCTCACACAACTTTGGTGACGGCTTC
CCTAAGGCATTCGGCATCCAGTACACAGACAAGGACAATAAATTACAGTACTGTCATGAGACATCATGGGGCGTTTCAAC
ACGTCTTATCGGAGCTATCATCATGGTTCACGGAGATGACTCAGGACTCGTGCTTCCACCAAAGATTGCACCAACACAGG
TTATGGTCATTCCAATCCAGCAGCAGAAGGACGGCGTCCTTGACAAGGCATACGACCTCAAGGACAAGCTCTCAAAGGAT
TTCCGTGTGAAAATCGATGCAACCGACAAGACACCTGGCTGGAAGTTCGCAGCACAGGAGGTTCAGGGAATCTCAACACG
TATCGAGATTGGACCAAAGGATATCGAGAAAAACCAGTGCGTTATCGTCCGCCGAGACACAAGAGAGAAAATCGTTGTCT
CACTTGATGAGGTCAATGAGAAGCTCGCAGAGGTACTTGAGACAATGCAGAATGACATGCTCGAGAAGGCAAAGGCATTC
CTTGCAAGCCATATAAACGATGCACACGACTACAATGAGTTCAAGGCAATCGCAGAGACAAAGCCTGGCTTTATCCGTGC
TATGTGGTGTGGAGACGAGGCCTGCGAGAATAAAATCAAAGAGGACACAACAGTTACAAGCCGTTGTATGCCATTTGGTG
ACCAGGAGCAGATTTCCGACGTATGCGTATGCTGTGGAAAGCCGGCGCATAAGCTCGTATACTGGGGCAAGGCATATTAA

Upstream 100 bases:

>100_bases
GAATAATATATGTTAGTAAAATTATGCGTGGTGCAGGGGCGTGATGAGACATAAAAGCCCTGTATATCATAGAATATTTC
AATAAAAAAGGAGAAAAGCA

Downstream 100 bases:

>100_bases
ATTCGCGCCTACGAGAATTTATTTCGCGAATTAATCATTACTTTTGTATATTATTATGATAAACAGCTCGCCGGAGGGGC
GCATTGACGCAGCATACGGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAKEKKLVEAITSMEEDFTQWYTDVVKKAELMDYSSVKGCMIFKPNGYAIWENIQKNLDAMFKETGVENVYMPMFIPESL
LQKEKDHVEGFAPEVAWVTQGGLETLQERLAVRPTSETLFCELFSKTVQSHRDLPKVYNQWCSVVRWEKTTRPFLRSSEF
LWQEGHTVHATAEEAEARTVQMLNIYADFCEKYLAIPMVKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
PKAFGIQYTDKDNKLQYCHETSWGVSTRLIGAIIMVHGDDSGLVLPPKIAPTQVMVIPIQQQKDGVLDKAYDLKDKLSKD
FRVKIDATDKTPGWKFAAQEVQGISTRIEIGPKDIEKNQCVIVRRDTREKIVVSLDEVNEKLAEVLETMQNDMLEKAKAF
LASHINDAHDYNEFKAIAETKPGFIRAMWCGDEACENKIKEDTTVTSRCMPFGDQEQISDVCVCCGKPAHKLVYWGKAY

Sequences:

>Translated_479_residues
MAKEKKLVEAITSMEEDFTQWYTDVVKKAELMDYSSVKGCMIFKPNGYAIWENIQKNLDAMFKETGVENVYMPMFIPESL
LQKEKDHVEGFAPEVAWVTQGGLETLQERLAVRPTSETLFCELFSKTVQSHRDLPKVYNQWCSVVRWEKTTRPFLRSSEF
LWQEGHTVHATAEEAEARTVQMLNIYADFCEKYLAIPMVKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
PKAFGIQYTDKDNKLQYCHETSWGVSTRLIGAIIMVHGDDSGLVLPPKIAPTQVMVIPIQQQKDGVLDKAYDLKDKLSKD
FRVKIDATDKTPGWKFAAQEVQGISTRIEIGPKDIEKNQCVIVRRDTREKIVVSLDEVNEKLAEVLETMQNDMLEKAKAF
LASHINDAHDYNEFKAIAETKPGFIRAMWCGDEACENKIKEDTTVTSRCMPFGDQEQISDVCVCCGKPAHKLVYWGKAY
>Mature_478_residues
AKEKKLVEAITSMEEDFTQWYTDVVKKAELMDYSSVKGCMIFKPNGYAIWENIQKNLDAMFKETGVENVYMPMFIPESLL
QKEKDHVEGFAPEVAWVTQGGLETLQERLAVRPTSETLFCELFSKTVQSHRDLPKVYNQWCSVVRWEKTTRPFLRSSEFL
WQEGHTVHATAEEAEARTVQMLNIYADFCEKYLAIPMVKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGFP
KAFGIQYTDKDNKLQYCHETSWGVSTRLIGAIIMVHGDDSGLVLPPKIAPTQVMVIPIQQQKDGVLDKAYDLKDKLSKDF
RVKIDATDKTPGWKFAAQEVQGISTRIEIGPKDIEKNQCVIVRRDTREKIVVSLDEVNEKLAEVLETMQNDMLEKAKAFL
ASHINDAHDYNEFKAIAETKPGFIRAMWCGDEACENKIKEDTTVTSRCMPFGDQEQISDVCVCCGKPAHKLVYWGKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=43.8133874239351, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71991342, Length=501, Percent_Identity=39.5209580838323, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71991346, Length=368, Percent_Identity=45.1086956521739, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6321808, Length=511, Percent_Identity=43.4442270058708, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24649468, Length=495, Percent_Identity=41.8181818181818, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24649466, Length=495, Percent_Identity=41.8181818181818, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_EUBR3 (C4ZC24)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002936448.1
- GeneID:   7963728
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_0523
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54429; Mature: 54297

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKKLVEAITSMEEDFTQWYTDVVKKAELMDYSSVKGCMIFKPNGYAIWENIQKNLDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
MFKETGVENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTQGGLETLQERLAVRPTSETLF
HHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCCCCHHHH
CELFSKTVQSHRDLPKVYNQWCSVVRWEKTTRPFLRSSEFLWQEGHTVHATAEEAEARTV
HHHHHHHHHHHCCHHHHHHHHHHHEECCHHCCHHHHCCHHHHHCCCEEEEEHHHHHHHHH
QMLNIYADFCEKYLAIPMVKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHEEHHHHHHCCHHHHCCCCCCCCCCC
PKAFGIQYTDKDNKLQYCHETSWGVSTRLIGAIIMVHGDDSGLVLPPKIAPTQVMVIPIQ
CHHCCCEECCCCCCEEEECCCCCCHHHHEEEEEEEEEECCCCEEECCCCCCCEEEEEEEC
QQKDGVLDKAYDLKDKLSKDFRVKIDATDKTPGWKFAAQEVQGISTRIEIGPKDIEKNQC
CCCCCCHHHHHCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCE
VIVRRDTREKIVVSLDEVNEKLAEVLETMQNDMLEKAKAFLASHINDAHDYNEFKAIAET
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
KPGFIRAMWCGDEACENKIKEDTTVTSRCMPFGDQEQISDVCVCCGKPAHKLVYWGKAY
CCCEEEEEECCCHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHEEEECCCC
>Mature Secondary Structure 
AKEKKLVEAITSMEEDFTQWYTDVVKKAELMDYSSVKGCMIFKPNGYAIWENIQKNLDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
MFKETGVENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTQGGLETLQERLAVRPTSETLF
HHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCCCCHHHH
CELFSKTVQSHRDLPKVYNQWCSVVRWEKTTRPFLRSSEFLWQEGHTVHATAEEAEARTV
HHHHHHHHHHHCCHHHHHHHHHHHEECCHHCCHHHHCCHHHHHCCCEEEEEHHHHHHHHH
QMLNIYADFCEKYLAIPMVKGRKTDKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHEEHHHHHHCCHHHHCCCCCCCCCCC
PKAFGIQYTDKDNKLQYCHETSWGVSTRLIGAIIMVHGDDSGLVLPPKIAPTQVMVIPIQ
CHHCCCEECCCCCCEEEECCCCCCHHHHEEEEEEEEEECCCCEEECCCCCCCEEEEEEEC
QQKDGVLDKAYDLKDKLSKDFRVKIDATDKTPGWKFAAQEVQGISTRIEIGPKDIEKNQC
CCCCCCHHHHHCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCE
VIVRRDTREKIVVSLDEVNEKLAEVLETMQNDMLEKAKAFLASHINDAHDYNEFKAIAET
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
KPGFIRAMWCGDEACENKIKEDTTVTSRCMPFGDQEQISDVCVCCGKPAHKLVYWGKAY
CCCEEEEEECCCHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA