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The map label for this gene is gph1 [H]

Identifier: 238922767

GI number: 238922767

Start: 318772

End: 320994

Strand: Direct

Name: gph1 [H]

Synonym: EUBREC_0355

Alternate gene names: 238922767

Gene position: 318772-320994 (Clockwise)

Preceding gene: 238922766

Following gene: 238922768

Centisome position: 9.24

GC content: 44.08

Gene sequence:

>2223_bases
ATGAAGCTAAGTGATTTATTACAATTTGATAACATAATTGTGCAATGTCATGATAATCCGGACGCAGATGCACTGGCAAG
CGGCTTTGGAGTCTATGAATATCTGAGGATGAATGGAAAAAGTCCCAGACTTGTATACGGAGGCAGGAATATCATACACA
AGAGCAATCTGGTGCTTATGGTAGACAGTCTTGGCATACCGATAGAGCATGTGGATTTTTTAGACAATCCGCAGCTGCTT
GTGACTGTCGATTGTCAGTACGGTGGTGGGAATGTCACATTTTTCAAGGCAGAAAATGTAGCTGTCATAGATCACCACAG
AGTATCGGGAGAGCTTCCTGCTATGAACCGTGTCATGAGCTATATGGGCTCATGTGCGACAATTGTGTGGGATATGCTGC
GTGAGGAGGGCGTAGAGATAAATCGAAACCTGGCAACAGCACTGTATTATGGACTTTACACCGACACCGGAGAATTTACC
GAGATAACACATTCGCTTGATAGGGACCTGCGCGATGAGGCGGATTTTGACAACACTATAGTGGCCAAATTCCGAAATGC
CAACATGTCACTTGAGGAGCTGGACATAGCCGCAACAGCATTGCTGGGGCGCGATTATATAGAGGAATATCGTCTGGCGA
TCGTAAAGGCAGGGGCATGTGACCCGAATGTACTCGGCATCATAAGCGATTTTGTGCTGGAGGTGGATGCTATTGACATA
TGTATGGTATTTTCAGTGATTAAGAATGGAGTGAAGCTTTCGTTTAGAAGCTGTATAAAAGAGGTGAGCGCAAGCGAAAT
GGCGCAGGAAGTGTGCAGGGACATAGGCTCAGGCGGAGGGCATTATTACAAGGCCGGTGGATTTATTCCGATGGATCTGC
TGATAGACAGCTATAATGTCTACTGCAAAGAAAAAGACGTGACACCAAGATTTCAGTACAGCAGCGATGGTACACACAAA
CGTCCTTCGGATTCTGCAATCAAATCATTATTGGAAGAGAGGATTTTTGATTATTTAAACGATACAAAGATAATTTACGG
AGAAGATTTTGATACGAGTGGCTTCAAAAAAGTGGATTATAAAAAACGCCCGATACCGATGGGATGTATCATTGCAAAGG
ATATACTGCCTGTGGGCTGCTGCATGGGAGTGCGGACAGCTAAGGGGGATATCTCCACACCGGTTAGTGAAGATACGGTA
GTCATAATAGGAGAAGACGGCAGTGTACGGATTTTAAATCTGGACAGGCTGAACAAAAGCTTCAGGATATACAAGGACTG
GCGGTTTACTGTGAAACAGACAGATTATGTCCCGAAGTTCAAGAATAAGGATACGGAGACTATAGTGGATGGAATGGCAC
ATGCCAGAGTGTGCATACCTGTTGAGGAGGATTTTTCAAGAGCATTTGTGCTAAAGCACAAGGTAAAGCTATTTAAGAAT
AAGGATGACAGCAGTTATATATCAGGCAGACCTGGAGATATTATGGTTCTGCCAAATGATGACAGGAATGAGGCATATAT
GATAAGCAAGACAGAATTTGAAAAGACACATATAGCAAAGGGTGAGGAGGAAAACCGTAAAAAAGCGGTCGTTTTCGACC
TTGATGGAACACTTCTCTATACACTTGAGGATTTAAAAAATGCAACAAACTATGCATTAAAACAAAATGGCATGCCGGAG
CGTACACTCGACGAGGTGCGCAGATTTGTGGGAAACGGTGTAAAGCTTCTGATGGAGCGCGCAGTGCCACAGGGGGCAGA
CAATCCAAAGTTTGAAAAGACATTTTCGGATTTCAAGGAATATTATGAGGCTCATTGCAATGACAATACAGCACCTTATG
ATGGAATCATGGAGCTTTTAAAGGAGCTTAAATTAAACGGCATAAAGCTTGCTATTGTTTCAAACAAGCTTGACCCGGCG
GTAAAGGAGCTCAATCAGCTTTATTTCAAGGAGTATATGACATCGGCAGTCGGCGAGATGGAGGAAGAGGGTATACGCAA
AAAGCCGGCACCTGATATGGTGCAGAAGGCACTTAAAGAGCTTCAGGTGAGTGCAGATGAAGCAATCTATGTGGGGGACT
CCGATGTGGATATCGCCACAGCGAAAAACTCAGGTCTGGAGTGCGTATCTGTCACATGGGGCTTCAGGGACGTAGAGTTT
TTGAAGGAGCATGGTGCAACAAATCTGATAGATGAACCGGTCGAATTGTTAAATTACGTATAA

Upstream 100 bases:

>100_bases
CTCGCTGCTGTTTCCGTACCTGAAATAATGTTATGATTTGAAGCAGCCATAAATACACATATGAGAAAATGAATTAATAT
AACTATTCAGGGAGCTAATA

Downstream 100 bases:

>100_bases
TCAGGAGAACTTTGATTTATGCTTTCAGAAAAGGGATTATTGATAATTGAAAAGCTGGCAGAGCACAACAATGAGCTTGT
CACATCAAAGGCTCTTGCTG

Product: putative phosphatase

Products: NA

Alternate protein names: PGP 1; PGPase 1 [H]

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MKLSDLLQFDNIIVQCHDNPDADALASGFGVYEYLRMNGKSPRLVYGGRNIIHKSNLVLMVDSLGIPIEHVDFLDNPQLL
VTVDCQYGGGNVTFFKAENVAVIDHHRVSGELPAMNRVMSYMGSCATIVWDMLREEGVEINRNLATALYYGLYTDTGEFT
EITHSLDRDLRDEADFDNTIVAKFRNANMSLEELDIAATALLGRDYIEEYRLAIVKAGACDPNVLGIISDFVLEVDAIDI
CMVFSVIKNGVKLSFRSCIKEVSASEMAQEVCRDIGSGGGHYYKAGGFIPMDLLIDSYNVYCKEKDVTPRFQYSSDGTHK
RPSDSAIKSLLEERIFDYLNDTKIIYGEDFDTSGFKKVDYKKRPIPMGCIIAKDILPVGCCMGVRTAKGDISTPVSEDTV
VIIGEDGSVRILNLDRLNKSFRIYKDWRFTVKQTDYVPKFKNKDTETIVDGMAHARVCIPVEEDFSRAFVLKHKVKLFKN
KDDSSYISGRPGDIMVLPNDDRNEAYMISKTEFEKTHIAKGEEENRKKAVVFDLDGTLLYTLEDLKNATNYALKQNGMPE
RTLDEVRRFVGNGVKLLMERAVPQGADNPKFEKTFSDFKEYYEAHCNDNTAPYDGIMELLKELKLNGIKLAIVSNKLDPA
VKELNQLYFKEYMTSAVGEMEEEGIRKKPAPDMVQKALKELQVSADEAIYVGDSDVDIATAKNSGLECVSVTWGFRDVEF
LKEHGATNLIDEPVELLNYV

Sequences:

>Translated_740_residues
MKLSDLLQFDNIIVQCHDNPDADALASGFGVYEYLRMNGKSPRLVYGGRNIIHKSNLVLMVDSLGIPIEHVDFLDNPQLL
VTVDCQYGGGNVTFFKAENVAVIDHHRVSGELPAMNRVMSYMGSCATIVWDMLREEGVEINRNLATALYYGLYTDTGEFT
EITHSLDRDLRDEADFDNTIVAKFRNANMSLEELDIAATALLGRDYIEEYRLAIVKAGACDPNVLGIISDFVLEVDAIDI
CMVFSVIKNGVKLSFRSCIKEVSASEMAQEVCRDIGSGGGHYYKAGGFIPMDLLIDSYNVYCKEKDVTPRFQYSSDGTHK
RPSDSAIKSLLEERIFDYLNDTKIIYGEDFDTSGFKKVDYKKRPIPMGCIIAKDILPVGCCMGVRTAKGDISTPVSEDTV
VIIGEDGSVRILNLDRLNKSFRIYKDWRFTVKQTDYVPKFKNKDTETIVDGMAHARVCIPVEEDFSRAFVLKHKVKLFKN
KDDSSYISGRPGDIMVLPNDDRNEAYMISKTEFEKTHIAKGEEENRKKAVVFDLDGTLLYTLEDLKNATNYALKQNGMPE
RTLDEVRRFVGNGVKLLMERAVPQGADNPKFEKTFSDFKEYYEAHCNDNTAPYDGIMELLKELKLNGIKLAIVSNKLDPA
VKELNQLYFKEYMTSAVGEMEEEGIRKKPAPDMVQKALKELQVSADEAIYVGDSDVDIATAKNSGLECVSVTWGFRDVEF
LKEHGATNLIDEPVELLNYV
>Mature_740_residues
MKLSDLLQFDNIIVQCHDNPDADALASGFGVYEYLRMNGKSPRLVYGGRNIIHKSNLVLMVDSLGIPIEHVDFLDNPQLL
VTVDCQYGGGNVTFFKAENVAVIDHHRVSGELPAMNRVMSYMGSCATIVWDMLREEGVEINRNLATALYYGLYTDTGEFT
EITHSLDRDLRDEADFDNTIVAKFRNANMSLEELDIAATALLGRDYIEEYRLAIVKAGACDPNVLGIISDFVLEVDAIDI
CMVFSVIKNGVKLSFRSCIKEVSASEMAQEVCRDIGSGGGHYYKAGGFIPMDLLIDSYNVYCKEKDVTPRFQYSSDGTHK
RPSDSAIKSLLEERIFDYLNDTKIIYGEDFDTSGFKKVDYKKRPIPMGCIIAKDILPVGCCMGVRTAKGDISTPVSEDTV
VIIGEDGSVRILNLDRLNKSFRIYKDWRFTVKQTDYVPKFKNKDTETIVDGMAHARVCIPVEEDFSRAFVLKHKVKLFKN
KDDSSYISGRPGDIMVLPNDDRNEAYMISKTEFEKTHIAKGEEENRKKAVVFDLDGTLLYTLEDLKNATNYALKQNGMPE
RTLDEVRRFVGNGVKLLMERAVPQGADNPKFEKTFSDFKEYYEAHCNDNTAPYDGIMELLKELKLNGIKLAIVSNKLDPA
VKELNQLYFKEYMTSAVGEMEEEGIRKKPAPDMVQKALKELQVSADEAIYVGDSDVDIATAKNSGLECVSVTWGFRDVEF
LKEHGATNLIDEPVELLNYV

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=209, Percent_Identity=28.7081339712919, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 83190; Mature: 83190

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDLLQFDNIIVQCHDNPDADALASGFGVYEYLRMNGKSPRLVYGGRNIIHKSNLVLM
CCHHHHHHHCCEEEEECCCCCHHHHHHHCHHHHHHHCCCCCCEEEECCCCEEECCCEEEE
VDSLGIPIEHVDFLDNPQLLVTVDCQYGGGNVTFFKAENVAVIDHHRVSGELPAMNRVMS
EECCCCCHHHCCCCCCCCEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHH
YMGSCATIVWDMLREEGVEINRNLATALYYGLYTDTGEFTEITHSLDRDLRDEADFDNTI
HHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
VAKFRNANMSLEELDIAATALLGRDYIEEYRLAIVKAGACDPNVLGIISDFVLEVDAIDI
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHH
CMVFSVIKNGVKLSFRSCIKEVSASEMAQEVCRDIGSGGGHYYKAGGFIPMDLLIDSYNV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEHHHEEECCCE
YCKEKDVTPRFQYSSDGTHKRPSDSAIKSLLEERIFDYLNDTKIIYGEDFDTSGFKKVDY
EEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCC
KKRPIPMGCIIAKDILPVGCCMGVRTAKGDISTPVSEDTVVIIGEDGSVRILNLDRLNKS
CCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEECCCCEEEEEHHHCCCC
FRIYKDWRFTVKQTDYVPKFKNKDTETIVDGMAHARVCIPVEEDFSRAFVLKHKVKLFKN
EEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHCC
KDDSSYISGRPGDIMVLPNDDRNEAYMISKTEFEKTHIAKGEEENRKKAVVFDLDGTLLY
CCCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHCCCCCCCCCCEEEEEECCCEEEE
TLEDLKNATNYALKQNGMPERTLDEVRRFVGNGVKLLMERAVPQGADNPKFEKTFSDFKE
EEHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YYEAHCNDNTAPYDGIMELLKELKLNGIKLAIVSNKLDPAVKELNQLYFKEYMTSAVGEM
HHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EEEGIRKKPAPDMVQKALKELQVSADEAIYVGDSDVDIATAKNSGLECVSVTWGFRDVEF
HHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCEEEEEECCCHHHHH
LKEHGATNLIDEPVELLNYV
HHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLSDLLQFDNIIVQCHDNPDADALASGFGVYEYLRMNGKSPRLVYGGRNIIHKSNLVLM
CCHHHHHHHCCEEEEECCCCCHHHHHHHCHHHHHHHCCCCCCEEEECCCCEEECCCEEEE
VDSLGIPIEHVDFLDNPQLLVTVDCQYGGGNVTFFKAENVAVIDHHRVSGELPAMNRVMS
EECCCCCHHHCCCCCCCCEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHH
YMGSCATIVWDMLREEGVEINRNLATALYYGLYTDTGEFTEITHSLDRDLRDEADFDNTI
HHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
VAKFRNANMSLEELDIAATALLGRDYIEEYRLAIVKAGACDPNVLGIISDFVLEVDAIDI
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHH
CMVFSVIKNGVKLSFRSCIKEVSASEMAQEVCRDIGSGGGHYYKAGGFIPMDLLIDSYNV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEHHHEEECCCE
YCKEKDVTPRFQYSSDGTHKRPSDSAIKSLLEERIFDYLNDTKIIYGEDFDTSGFKKVDY
EEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCC
KKRPIPMGCIIAKDILPVGCCMGVRTAKGDISTPVSEDTVVIIGEDGSVRILNLDRLNKS
CCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEECCCCEEEEEHHHCCCC
FRIYKDWRFTVKQTDYVPKFKNKDTETIVDGMAHARVCIPVEEDFSRAFVLKHKVKLFKN
EEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHCC
KDDSSYISGRPGDIMVLPNDDRNEAYMISKTEFEKTHIAKGEEENRKKAVVFDLDGTLLY
CCCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHCCCCCCCCCCEEEEEECCCEEEE
TLEDLKNATNYALKQNGMPERTLDEVRRFVGNGVKLLMERAVPQGADNPKFEKTFSDFKE
EEHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YYEAHCNDNTAPYDGIMELLKELKLNGIKLAIVSNKLDPAVKELNQLYFKEYMTSAVGEM
HHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EEEGIRKKPAPDMVQKALKELQVSADEAIYVGDSDVDIATAKNSGLECVSVTWGFRDVEF
HHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCEEEEEECCCHHHHH
LKEHGATNLIDEPVELLNYV
HHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]