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The map label for this gene is iscS [H]

Identifier: 238922732

GI number: 238922732

Start: 285468

End: 286661

Strand: Direct

Name: iscS [H]

Synonym: EUBREC_0308

Alternate gene names: 238922732

Gene position: 285468-286661 (Clockwise)

Preceding gene: 238922731

Following gene: 238922733

Centisome position: 8.28

GC content: 48.49

Gene sequence:

>1194_bases
ATGAGCAGATTAATTTATTTGGATAATGCGGCAACCACACAGGTATATCCGGAGGTTGTGGATGCAATGCTTCCTTACTT
TACAGAGCATTACGGCAATCCATCAGCTATATACAGCTTTGCAGATGAGGCAGAAAGAGCAGTGGATCATGCCAGAGGAG
AGGTCGCAGACCTTATCGGAGCCAAGAAGGATGAAATATATTTCACAGGCGGCGGCAGCGAGTCCGACAACTGGGCATTA
AAGGCCACAGCAGAGGCATATGCTGCAAAAGGAAAGCACATCATCACATCAGCCATCGAGCATCATGCAATTCTTCACAC
AGCACAGTGGCTTGAGAAGCATGGCTTTGAGGTGACATATGTGGGTGTTGATGAGGACGGAAAAATCAAGCTTGATGAGC
TTAAGGCAGCCATCAGACCTGACACAATCCTTATCTCAGTTATGGCGGCAAACAACGAGATTGGAACTATCCAGCCGCTT
AAGGAGATCGGAGAGATAGCAAAGGAGCATGGCATTTTGTTCCACACAGATGCAGTTCAGGCATTCGGACACATTCCGAT
TAATGTAGATGATATGCATATCGACATGCTCAGCGCCAGCGGTCACAAGCTCAACGGGCCAAAGGGCATCGGCGTGATGT
ATATCAGAAAGGGCGTAAAAATCCGCTCATTTATCCACGGCGGAGCACAGGAGCGCCAGAGACGTGCGGGAACACACAAT
GTGCCGGGTATCGTAGGACTCGGCAAGGCAGTAGAGCTTGCAAAGGCTAACATGGCAGAGCGCAGCGCATATGAGACAAA
GCTCAGAGACCACCTCATCGAGAGAGTGACAAGCGAGATTCCATATACACGCTTAAATGGACACAGAACAGACAGACTGC
CAAACAATGCAAACTTCTGCTTCAGATTTATCGAGGGAGAGTCAATGCTTATCCTGCTTGACCAGGCAGGCATATGCGGC
AGCTCAGGTTCAGCCTGCACATCAGGCTCACTCGACCCATCACATGTGCTCCTTGCAATAGGACTGCCACATGAGATAGC
ACACGGCTCACTGCGTCTTACATTATCAGAGAAGACCACTCTTGAGGATATCGACTATACAGTGGACAAGTTAAAGGAAA
TCATAGCAAGGCTCAGAAGCATGTCACCGTTATACGAGGATTTCGTAAAGAAAAACGGCAAAGCAGAGGCATAA

Upstream 100 bases:

>100_bases
ATCTCAGGTGTGGTTGACAATATCACCTTGCAGGATCTGGTAGACTGGCAGAACGAGAAGAATGGAAATTATGTCATATA
AAGAAAGAAGGTTATCATAG

Downstream 100 bases:

>100_bases
ATGATCAGGTCAAAATTATAGTGACTATCACAACATTAATATATACATGAAAATTAGGAGGAACTAAGGACATGGCATAC
AGTGAAAAGGTAATGGACCA

Product: cysteine desulfurase NifS

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSRLIYLDNAATTQVYPEVVDAMLPYFTEHYGNPSAIYSFADEAERAVDHARGEVADLIGAKKDEIYFTGGGSESDNWAL
KATAEAYAAKGKHIITSAIEHHAILHTAQWLEKHGFEVTYVGVDEDGKIKLDELKAAIRPDTILISVMAANNEIGTIQPL
KEIGEIAKEHGILFHTDAVQAFGHIPINVDDMHIDMLSASGHKLNGPKGIGVMYIRKGVKIRSFIHGGAQERQRRAGTHN
VPGIVGLGKAVELAKANMAERSAYETKLRDHLIERVTSEIPYTRLNGHRTDRLPNNANFCFRFIEGESMLILLDQAGICG
SSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLTLSEKTTLEDIDYTVDKLKEIIARLRSMSPLYEDFVKKNGKAEA

Sequences:

>Translated_397_residues
MSRLIYLDNAATTQVYPEVVDAMLPYFTEHYGNPSAIYSFADEAERAVDHARGEVADLIGAKKDEIYFTGGGSESDNWAL
KATAEAYAAKGKHIITSAIEHHAILHTAQWLEKHGFEVTYVGVDEDGKIKLDELKAAIRPDTILISVMAANNEIGTIQPL
KEIGEIAKEHGILFHTDAVQAFGHIPINVDDMHIDMLSASGHKLNGPKGIGVMYIRKGVKIRSFIHGGAQERQRRAGTHN
VPGIVGLGKAVELAKANMAERSAYETKLRDHLIERVTSEIPYTRLNGHRTDRLPNNANFCFRFIEGESMLILLDQAGICG
SSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLTLSEKTTLEDIDYTVDKLKEIIARLRSMSPLYEDFVKKNGKAEA
>Mature_396_residues
SRLIYLDNAATTQVYPEVVDAMLPYFTEHYGNPSAIYSFADEAERAVDHARGEVADLIGAKKDEIYFTGGGSESDNWALK
ATAEAYAAKGKHIITSAIEHHAILHTAQWLEKHGFEVTYVGVDEDGKIKLDELKAAIRPDTILISVMAANNEIGTIQPLK
EIGEIAKEHGILFHTDAVQAFGHIPINVDDMHIDMLSASGHKLNGPKGIGVMYIRKGVKIRSFIHGGAQERQRRAGTHNV
PGIVGLGKAVELAKANMAERSAYETKLRDHLIERVTSEIPYTRLNGHRTDRLPNNANFCFRFIEGESMLILLDQAGICGS
SGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLTLSEKTTLEDIDYTVDKLKEIIARLRSMSPLYEDFVKKNGKAEA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=387, Percent_Identity=47.0284237726098, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=36.5617433414044, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=46.8193384223919, Blast_Score=344, Evalue=5e-96,
Organism=Escherichia coli, GI1787970, Length=399, Percent_Identity=27.3182957393484, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=27.75, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=44.0414507772021, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=31.0769230769231, Blast_Score=157, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=388, Percent_Identity=46.3917525773196, Blast_Score=335, Evalue=9e-93,
Organism=Drosophila melanogaster, GI20129463, Length=387, Percent_Identity=44.9612403100775, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43519; Mature: 43388

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIYLDNAATTQVYPEVVDAMLPYFTEHYGNPSAIYSFADEAERAVDHARGEVADLIG
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHC
AKKDEIYFTGGGSESDNWALKATAEAYAAKGKHIITSAIEHHAILHTAQWLEKHGFEVTY
CCCCEEEEECCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGVDEDGKIKLDELKAAIRPDTILISVMAANNEIGTIQPLKEIGEIAKEHGILFHTDAVQ
EEECCCCCEEHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEHHHH
AFGHIPINVDDMHIDMLSASGHKLNGPKGIGVMYIRKGVKIRSFIHGGAQERQRRAGTHN
HHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCEEHHHCCCHHHHHHHCCCCC
VPGIVGLGKAVELAKANMAERSAYETKLRDHLIERVTSEIPYTRLNGHRTDRLPNNANFC
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
FRFIEGESMLILLDQAGICGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLTLSEKTT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCCEEEEEECCCCC
LEDIDYTVDKLKEIIARLRSMSPLYEDFVKKNGKAEA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRLIYLDNAATTQVYPEVVDAMLPYFTEHYGNPSAIYSFADEAERAVDHARGEVADLIG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHC
AKKDEIYFTGGGSESDNWALKATAEAYAAKGKHIITSAIEHHAILHTAQWLEKHGFEVTY
CCCCEEEEECCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGVDEDGKIKLDELKAAIRPDTILISVMAANNEIGTIQPLKEIGEIAKEHGILFHTDAVQ
EEECCCCCEEHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEHHHH
AFGHIPINVDDMHIDMLSASGHKLNGPKGIGVMYIRKGVKIRSFIHGGAQERQRRAGTHN
HHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCEEHHHCCCHHHHHHHCCCCC
VPGIVGLGKAVELAKANMAERSAYETKLRDHLIERVTSEIPYTRLNGHRTDRLPNNANFC
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
FRFIEGESMLILLDQAGICGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLTLSEKTT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCCEEEEEECCCCC
LEDIDYTVDKLKEIIARLRSMSPLYEDFVKKNGKAEA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]