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The map label for this gene is nfo [H]

Identifier: 238922623

GI number: 238922623

Start: 189076

End: 189960

Strand: Direct

Name: nfo [H]

Synonym: EUBREC_0198

Alternate gene names: 238922623

Gene position: 189076-189960 (Clockwise)

Preceding gene: 238922621

Following gene: 238922624

Centisome position: 5.48

GC content: 45.99

Gene sequence:

>885_bases
ATGAAACAGAGGAAAAATATGAGTGATATAAAATTAGGCTGTCACGTAGGCATGGCAGGAAAAGATATGTTTCTGGCATC
AGCCAGGGAAGCAGCGTCATATGGCGCGAATGTGTTTATGCTCTACACCGGGGCACCACAGAACACGAGAAGAAAGGAAA
TCTTTGAGCTCAACATAGACGCAGGCTGGGAATATGCACATGAGCATGGGATAAATGAGATAGTGGTCCACGCGCCATAC
ATCATAAATCTGGCAAACACCGTAAAGCCTGAGACATACGAGCTGGCAGTGGAGTTTCTGGAAAAGGAGATAGTAAGGAC
CGCGGCAATGAAGAGCCGCATACTGGTGCTTCATCCGGGAAGTCATGTAAATGCCGGTGAGCAGGCTGGAATCGCACAGA
TTGTAAAGGGACTTAACACAGTGCTCAATCAGAATGATGACGATGTGTTTATCGCACTTGAGACGATGGCAGGAAAGGGC
AGTGAAATAGGCCGTTCCTTTGAGGAGCTTAAGGCAATCTATGATGGAGTGGACAGGAAGGAAAGGCTGCGTGTCTGCTT
TGATACGTGCCATGTAAATGATGCAGGATACGATATTGTGAACCATTATGATGAGGTTTTTGCAGAGTTTGACAGGGTGA
TAGGGCTTGAGCAGATAGCGGTATTTCATGTGAATGACAGCTTAAATCCTCTTGGAGCACACAAGGACAGGCATGCAAAT
ATAGGCAAGGGAACCATCGGCTATGATACACTGCACCGCCTGGTGCATGATGAGACCTTTGCAAATGTACCAAAGATTCT
GGAAACACCATGGCTTTGTGAGGAGAGCTCTGCGAAGAAGACTATTCCTCCTTATAAAGAGGAAATTGAATGGCTTCTTA
AATAA

Upstream 100 bases:

>100_bases
ATGTCAATGACTTTTGCTATGTTTAGTAAAAATTTAGAATATTTAGATAGAGTTATCAGGGATGTAGTGTTATAGTAGAA
ATAATAGATATATAGATTCA

Downstream 100 bases:

>100_bases
GAAAGCAGCTAAAAATGTATGGAGAGGTTTGGTATTACAGACAGTAAGATAATGGAGGCATAATAGATGAAGGAAAAAAC
CTACGAGGGAACTAAAAAAA

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKQRKNMSDIKLGCHVGMAGKDMFLASAREAASYGANVFMLYTGAPQNTRRKEIFELNIDAGWEYAHEHGINEIVVHAPY
IINLANTVKPETYELAVEFLEKEIVRTAAMKSRILVLHPGSHVNAGEQAGIAQIVKGLNTVLNQNDDDVFIALETMAGKG
SEIGRSFEELKAIYDGVDRKERLRVCFDTCHVNDAGYDIVNHYDEVFAEFDRVIGLEQIAVFHVNDSLNPLGAHKDRHAN
IGKGTIGYDTLHRLVHDETFANVPKILETPWLCEESSAKKTIPPYKEEIEWLLK

Sequences:

>Translated_294_residues
MKQRKNMSDIKLGCHVGMAGKDMFLASAREAASYGANVFMLYTGAPQNTRRKEIFELNIDAGWEYAHEHGINEIVVHAPY
IINLANTVKPETYELAVEFLEKEIVRTAAMKSRILVLHPGSHVNAGEQAGIAQIVKGLNTVLNQNDDDVFIALETMAGKG
SEIGRSFEELKAIYDGVDRKERLRVCFDTCHVNDAGYDIVNHYDEVFAEFDRVIGLEQIAVFHVNDSLNPLGAHKDRHAN
IGKGTIGYDTLHRLVHDETFANVPKILETPWLCEESSAKKTIPPYKEEIEWLLK
>Mature_294_residues
MKQRKNMSDIKLGCHVGMAGKDMFLASAREAASYGANVFMLYTGAPQNTRRKEIFELNIDAGWEYAHEHGINEIVVHAPY
IINLANTVKPETYELAVEFLEKEIVRTAAMKSRILVLHPGSHVNAGEQAGIAQIVKGLNTVLNQNDDDVFIALETMAGKG
SEIGRSFEELKAIYDGVDRKERLRVCFDTCHVNDAGYDIVNHYDEVFAEFDRVIGLEQIAVFHVNDSLNPLGAHKDRHAN
IGKGTIGYDTLHRLVHDETFANVPKILETPWLCEESSAKKTIPPYKEEIEWLLK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=289, Percent_Identity=33.9100346020761, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=32.319391634981, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322735, Length=300, Percent_Identity=31.6666666666667, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33008; Mature: 33008

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRKNMSDIKLGCHVGMAGKDMFLASAREAASYGANVFMLYTGAPQNTRRKEIFELNID
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECC
AGWEYAHEHGINEIVVHAPYIINLANTVKPETYELAVEFLEKEIVRTAAMKSRILVLHPG
CCCHHHHHCCCCEEEEECCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
SHVNAGEQAGIAQIVKGLNTVLNQNDDDVFIALETMAGKGSEIGRSFEELKAIYDGVDRK
CCCCCCHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHH
ERLRVCFDTCHVNDAGYDIVNHYDEVFAEFDRVIGLEQIAVFHVNDSLNPLGAHKDRHAN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCCCCC
IGKGTIGYDTLHRLVHDETFANVPKILETPWLCEESSAKKTIPPYKEEIEWLLK
CCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKQRKNMSDIKLGCHVGMAGKDMFLASAREAASYGANVFMLYTGAPQNTRRKEIFELNID
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECC
AGWEYAHEHGINEIVVHAPYIINLANTVKPETYELAVEFLEKEIVRTAAMKSRILVLHPG
CCCHHHHHCCCCEEEEECCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
SHVNAGEQAGIAQIVKGLNTVLNQNDDDVFIALETMAGKGSEIGRSFEELKAIYDGVDRK
CCCCCCHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHH
ERLRVCFDTCHVNDAGYDIVNHYDEVFAEFDRVIGLEQIAVFHVNDSLNPLGAHKDRHAN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCCCCC
IGKGTIGYDTLHRLVHDETFANVPKILETPWLCEESSAKKTIPPYKEEIEWLLK
CCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA