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The map label for this gene is cls [C]

Identifier: 238922624

GI number: 238922624

Start: 190027

End: 191586

Strand: Direct

Name: cls [C]

Synonym: EUBREC_0199

Alternate gene names: 238922624

Gene position: 190027-191586 (Clockwise)

Preceding gene: 238922623

Following gene: 238922625

Centisome position: 5.51

GC content: 45.83

Gene sequence:

>1560_bases
ATGAAGGAAAAAACCTACGAGGGAACTAAAAAAACACAGAATGGTTTAAAGAGAATGTTTTTTTCAGTGCTTGCGATATT
ATTGCAGGCAGTTTTCATGGTCTATATGTTTACAAGGCTAAATGAATATGCGGCAGCAATAAACACGACCACAAGCGTTC
TGGCAATCGTACTTGTGCTCGGACTGTACAATCGTGAGCAGACCGCATCCATGACTACACCATGGATTATACTGATACTG
GCATTTCCGATAATGGGAGTGTCACTTTACCTGTTAGTCGGACTAAACGGCGCACCGAATAAGATGCGTGCACGGTTTGA
AGAGGTGGATTCCATGCTTCTTCCATGTCTGCCTGAAAACGCAGACATACTATCCGATATGTATGAAAAGGTGCCACAGG
CAGCGGGAATTTCAACATACCTGGCCAAAAATGCACTTTATCCGGTATATCAGAACACGGATGTGACATATTATGATCAG
GCGTCAAAGGGACTCGAAGCGCAGCTTAGTGATTTATCAAAGGCAAAGAAATTTATTTTCATGGAGTATCACGCCATAGA
GGACAAGGAATCGTGGCACCGTATCCAGAAGGTGCTCACGGAGCGCGTGCAGGCAGGAGTCGAGGTAAGAGTGTTTTATG
ATGATATGGGCTCAATCTTTTTCATAGACAGGGATTTCTCGGAAAAGATGGAGAAGCTTGGCATCAAATGCAGGGTGTTC
AACCCGGTTGCACCTGCCTTTAACATGTTTTTAAACAATCGTGACCATAGAAAAATCACGGTAATTGATGGAAAAATCGC
ATATACCGGCGGCTATAATCTGGCAAATGAGTACTTCAATGTGACACACCCATACGGAATCTGGAAGGATACGGGAATCA
GGCTCGAGGGAGATGCTGTCAAGTCGTTTACCATCGCATTTCTCGAGATGTGGAATGCATCAGAGCGCAAGGTACCGCGC
GAGGTGGATGTATCGCAATATCTGCTACACTATGATTATGAGGCAAAACAGAGTGGATATATCCAGCCATATGCAGACAG
CCCTCTCGATAACGAGCAGGTGGGGGAGGATGTCTATATCAGCATGGCAAACAAGGCACAGCGTTATTGCTGGTTTATCA
CTCCTTACCTTATCATTACCGATGAGATGAGCCATGCGCTGACACTTGCCGCCAAGCGTGGAGTTGACGTGCGCATCATC
ACGCCGGGCATACCTGATAAGAAACCAATATACAGCGTGACACGCTCCTTCTACAACCAGCTTGTGAAGCATGGTGTGAG
GATTTTTGAGTGGACACCGGGCTTTTGCCATGCAAAGATGAGTGTGGCAGACGATATAATGGCAACCTGCGGTACCATAA
ATCTTGACTATAGAAGCCTTTATCATCACTTTGAAAATGGCTGTTTTATGGCGGATTGTGAAGTGGTGCATGAAATCAGG
GATGATTTTATAAGGCTTTTCACTGAGTCAGCGGAGGTGACGGAGAAGTACAGGACAGGAAGAAGTGCAAGGCGAAGGCT
GGGGCAGTCAATTATGCGGCTGTTTGCAGGGCTATTGTAG

Upstream 100 bases:

>100_bases
TTATAAAGAGGAAATTGAATGGCTTCTTAAATAAGAAAGCAGCTAAAAATGTATGGAGAGGTTTGGTATTACAGACAGTA
AGATAATGGAGGCATAATAG

Downstream 100 bases:

>100_bases
ACTGAAAATAATGTGTATCAGGGAAGTGAGTGTATATGAGTGATTACGACAGCAAAATCATCAGAAAGCAGATAAGAGTG
TATGGCTCTGTGCAGGGGGT

Product: possible cardiolipin synthase (phospholipase D family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKEKTYEGTKKTQNGLKRMFFSVLAILLQAVFMVYMFTRLNEYAAAINTTTSVLAIVLVLGLYNREQTASMTTPWIILIL
AFPIMGVSLYLLVGLNGAPNKMRARFEEVDSMLLPCLPENADILSDMYEKVPQAAGISTYLAKNALYPVYQNTDVTYYDQ
ASKGLEAQLSDLSKAKKFIFMEYHAIEDKESWHRIQKVLTERVQAGVEVRVFYDDMGSIFFIDRDFSEKMEKLGIKCRVF
NPVAPAFNMFLNNRDHRKITVIDGKIAYTGGYNLANEYFNVTHPYGIWKDTGIRLEGDAVKSFTIAFLEMWNASERKVPR
EVDVSQYLLHYDYEAKQSGYIQPYADSPLDNEQVGEDVYISMANKAQRYCWFITPYLIITDEMSHALTLAAKRGVDVRII
TPGIPDKKPIYSVTRSFYNQLVKHGVRIFEWTPGFCHAKMSVADDIMATCGTINLDYRSLYHHFENGCFMADCEVVHEIR
DDFIRLFTESAEVTEKYRTGRSARRRLGQSIMRLFAGLL

Sequences:

>Translated_519_residues
MKEKTYEGTKKTQNGLKRMFFSVLAILLQAVFMVYMFTRLNEYAAAINTTTSVLAIVLVLGLYNREQTASMTTPWIILIL
AFPIMGVSLYLLVGLNGAPNKMRARFEEVDSMLLPCLPENADILSDMYEKVPQAAGISTYLAKNALYPVYQNTDVTYYDQ
ASKGLEAQLSDLSKAKKFIFMEYHAIEDKESWHRIQKVLTERVQAGVEVRVFYDDMGSIFFIDRDFSEKMEKLGIKCRVF
NPVAPAFNMFLNNRDHRKITVIDGKIAYTGGYNLANEYFNVTHPYGIWKDTGIRLEGDAVKSFTIAFLEMWNASERKVPR
EVDVSQYLLHYDYEAKQSGYIQPYADSPLDNEQVGEDVYISMANKAQRYCWFITPYLIITDEMSHALTLAAKRGVDVRII
TPGIPDKKPIYSVTRSFYNQLVKHGVRIFEWTPGFCHAKMSVADDIMATCGTINLDYRSLYHHFENGCFMADCEVVHEIR
DDFIRLFTESAEVTEKYRTGRSARRRLGQSIMRLFAGLL
>Mature_519_residues
MKEKTYEGTKKTQNGLKRMFFSVLAILLQAVFMVYMFTRLNEYAAAINTTTSVLAIVLVLGLYNREQTASMTTPWIILIL
AFPIMGVSLYLLVGLNGAPNKMRARFEEVDSMLLPCLPENADILSDMYEKVPQAAGISTYLAKNALYPVYQNTDVTYYDQ
ASKGLEAQLSDLSKAKKFIFMEYHAIEDKESWHRIQKVLTERVQAGVEVRVFYDDMGSIFFIDRDFSEKMEKLGIKCRVF
NPVAPAFNMFLNNRDHRKITVIDGKIAYTGGYNLANEYFNVTHPYGIWKDTGIRLEGDAVKSFTIAFLEMWNASERKVPR
EVDVSQYLLHYDYEAKQSGYIQPYADSPLDNEQVGEDVYISMANKAQRYCWFITPYLIITDEMSHALTLAAKRGVDVRII
TPGIPDKKPIYSVTRSFYNQLVKHGVRIFEWTPGFCHAKMSVADDIMATCGTINLDYRSLYHHFENGCFMADCEVVHEIR
DDFIRLFTESAEVTEKYRTGRSARRRLGQSIMRLFAGLL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=504, Percent_Identity=26.3888888888889, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1787007, Length=288, Percent_Identity=32.2916666666667, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59529; Mature: 59529

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTYEGTKKTQNGLKRMFFSVLAILLQAVFMVYMFTRLNEYAAAINTTTSVLAIVLVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYNREQTASMTTPWIILILAFPIMGVSLYLLVGLNGAPNKMRARFEEVDSMLLPCLPEN
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ADILSDMYEKVPQAAGISTYLAKNALYPVYQNTDVTYYDQASKGLEAQLSDLSKAKKFIF
HHHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHEEE
MEYHAIEDKESWHRIQKVLTERVQAGVEVRVFYDDMGSIFFIDRDFSEKMEKLGIKCRVF
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHHHHHCCCEEEEE
NPVAPAFNMFLNNRDHRKITVIDGKIAYTGGYNLANEYFNVTHPYGIWKDTGIRLEGDAV
CCHHHHHHHHHCCCCCCEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCCEEEECCHH
KSFTIAFLEMWNASERKVPREVDVSQYLLHYDYEAKQSGYIQPYADSPLDNEQVGEDVYI
HHHHHHHHHHHCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCEEE
SMANKAQRYCWFITPYLIITDEMSHALTLAAKRGVDVRIITPGIPDKKPIYSVTRSFYNQ
EEHHCCCCEEEEEECEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
LVKHGVRIFEWTPGFCHAKMSVADDIMATCGTINLDYRSLYHHFENGCFMADCEVVHEIR
HHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHH
DDFIRLFTESAEVTEKYRTGRSARRRLGQSIMRLFAGLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEKTYEGTKKTQNGLKRMFFSVLAILLQAVFMVYMFTRLNEYAAAINTTTSVLAIVLVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYNREQTASMTTPWIILILAFPIMGVSLYLLVGLNGAPNKMRARFEEVDSMLLPCLPEN
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ADILSDMYEKVPQAAGISTYLAKNALYPVYQNTDVTYYDQASKGLEAQLSDLSKAKKFIF
HHHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHEEE
MEYHAIEDKESWHRIQKVLTERVQAGVEVRVFYDDMGSIFFIDRDFSEKMEKLGIKCRVF
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHHHHHCCCEEEEE
NPVAPAFNMFLNNRDHRKITVIDGKIAYTGGYNLANEYFNVTHPYGIWKDTGIRLEGDAV
CCHHHHHHHHHCCCCCCEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCCEEEECCHH
KSFTIAFLEMWNASERKVPREVDVSQYLLHYDYEAKQSGYIQPYADSPLDNEQVGEDVYI
HHHHHHHHHHHCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCEEE
SMANKAQRYCWFITPYLIITDEMSHALTLAAKRGVDVRIITPGIPDKKPIYSVTRSFYNQ
EEHHCCCCEEEEEECEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
LVKHGVRIFEWTPGFCHAKMSVADDIMATCGTINLDYRSLYHHFENGCFMADCEVVHEIR
HHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHH
DDFIRLFTESAEVTEKYRTGRSARRRLGQSIMRLFAGLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]