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The map label for this gene is scrFIAM [H]

Identifier: 238922589

GI number: 238922589

Start: 156742

End: 157920

Strand: Direct

Name: scrFIAM [H]

Synonym: EUBREC_0163

Alternate gene names: 238922589

Gene position: 156742-157920 (Clockwise)

Preceding gene: 238922588

Following gene: 238922590

Centisome position: 4.54

GC content: 35.79

Gene sequence:

>1179_bases
ATGTGGTACATATTTTTTCCCGGAGGCAATTATATGAAACAGATGCATTTAAGAGTAGATGATGCATTGTATGAAGAACT
TAATACATATTCTGTTGAAACAGAACAATCAATGCAAGCTTGTGTAAGAGAAGCAGTAGTATATTATATTACAGATAAGA
AAAAGAAACAGATACCTATTTTAAAATCTCAATTTACTTTTATTGATCTCTTTGCGGGAATTGGAGGAATGAGACTTGCA
TATGAGAATGTTGGTGGAAGATGCGTATATTCCAACGAATGGAATAAGTATAGTCAGCAAACATATTATGCTAATTTTGG
GGAACAACCGGAAGGCGATATTACAAAGGTGGACGCTAAAACAATTCCTGATCATGATATATTGGTTGCAGGGTTCCCGT
GTCAACCGTTTTCTATTGCGGGCGTCTCGAAGAAGATTAGTTTGGGAAGAAAGACTGGGTTTGAAGATAAAACACAAGGA
ACATTATTCTTTGATGTTTGCAGAATTTTAAAAGAAAAGCGCCCCAAAGCGTTTATGCTTGAAAATGTAAAAAATTTGAA
GAGTCATGATAAAGGAAGGACCTTTAAAACGATTCTAGAATCATTAGATGAGTTGAAATATAAGGTTTTCTTTGCAGTGT
TGGACGGACAGAATTTTGTCCCGCAACATAGAGAGAGAATTATAATTGTTGGCTTTGACATGGAGAGATATGGTGATGAT
ATTGAGTTTGATTTTGACATTACGCCAGTCAATCCGAAACCAGTGATGCGAGACATTTTAGAAAAAAAGGTAGATGATAA
ATATACATTGTCTGATAATTTATGGACATATCTTCAGAATTATGCTGCTAAACATAGGGCAGCTGGTAATGGATTTGGTT
ATGGAATTGCGCCTTTGGATGGAGTATCTAGAACTATTAGTGCACGATATCATAAGGATGGATCAGAAATATTAATAGCT
CAAAAGGGAAAAAATCCGAGGAGGTTGACTCCGCGTGAGTGCGCGAGATTACAGGGATTTCCAGAAAGCTTTGTAATACC
GGTGTCTGATACGCAAGCGTATAGACAGTTTGGAAATTCGGTAGTTGTTCCATTAATAGAGAATGTTGCAAAACTGATTG
TAGAAAAAATTGATTTATTAGAATCGGATGTTCAGAATGAAAAAAGGAAGGCGATTTAA

Upstream 100 bases:

>100_bases
ACGTGATGTCACAACAAAAAAATGGCGATTAAAATAACGAACATATATTCGAAAATCATATTGCTTTTTTGTAAATGATG
CTGTATAATGTAAGGGAAAC

Downstream 100 bases:

>100_bases
TGTCAACAGAAATACTAAACAAAGCAATAGAATCAACTGTTGCAAGTGAATTGGCTTTTTGCAAATTTTTATCTGCGAAT
GATACTGGAGCGACAGGTGG

Product: modification methylase

Products: NA

Alternate protein names: M.ScrFI-A; M.ScrFIA; Cytosine-specific methyltransferase ScrFIA [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MWYIFFPGGNYMKQMHLRVDDALYEELNTYSVETEQSMQACVREAVVYYITDKKKKQIPILKSQFTFIDLFAGIGGMRLA
YENVGGRCVYSNEWNKYSQQTYYANFGEQPEGDITKVDAKTIPDHDILVAGFPCQPFSIAGVSKKISLGRKTGFEDKTQG
TLFFDVCRILKEKRPKAFMLENVKNLKSHDKGRTFKTILESLDELKYKVFFAVLDGQNFVPQHRERIIIVGFDMERYGDD
IEFDFDITPVNPKPVMRDILEKKVDDKYTLSDNLWTYLQNYAAKHRAAGNGFGYGIAPLDGVSRTISARYHKDGSEILIA
QKGKNPRRLTPRECARLQGFPESFVIPVSDTQAYRQFGNSVVVPLIENVAKLIVEKIDLLESDVQNEKRKAI

Sequences:

>Translated_392_residues
MWYIFFPGGNYMKQMHLRVDDALYEELNTYSVETEQSMQACVREAVVYYITDKKKKQIPILKSQFTFIDLFAGIGGMRLA
YENVGGRCVYSNEWNKYSQQTYYANFGEQPEGDITKVDAKTIPDHDILVAGFPCQPFSIAGVSKKISLGRKTGFEDKTQG
TLFFDVCRILKEKRPKAFMLENVKNLKSHDKGRTFKTILESLDELKYKVFFAVLDGQNFVPQHRERIIIVGFDMERYGDD
IEFDFDITPVNPKPVMRDILEKKVDDKYTLSDNLWTYLQNYAAKHRAAGNGFGYGIAPLDGVSRTISARYHKDGSEILIA
QKGKNPRRLTPRECARLQGFPESFVIPVSDTQAYRQFGNSVVVPLIENVAKLIVEKIDLLESDVQNEKRKAI
>Mature_392_residues
MWYIFFPGGNYMKQMHLRVDDALYEELNTYSVETEQSMQACVREAVVYYITDKKKKQIPILKSQFTFIDLFAGIGGMRLA
YENVGGRCVYSNEWNKYSQQTYYANFGEQPEGDITKVDAKTIPDHDILVAGFPCQPFSIAGVSKKISLGRKTGFEDKTQG
TLFFDVCRILKEKRPKAFMLENVKNLKSHDKGRTFKTILESLDELKYKVFFAVLDGQNFVPQHRERIIIVGFDMERYGDD
IEFDFDITPVNPKPVMRDILEKKVDDKYTLSDNLWTYLQNYAAKHRAAGNGFGYGIAPLDGVSRTISARYHKDGSEILIA
QKGKNPRRLTPRECARLQGFPESFVIPVSDTQAYRQFGNSVVVPLIENVAKLIVEKIDLLESDVQNEKRKAI

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=387, Percent_Identity=48.578811369509, Blast_Score=317, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 44925; Mature: 44925

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYIFFPGGNYMKQMHLRVDDALYEELNTYSVETEQSMQACVREAVVYYITDKKKKQIPI
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCC
LKSQFTFIDLFAGIGGMRLAYENVGGRCVYSNEWNKYSQQTYYANFGEQPEGDITKVDAK
HHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHCCEEEECCCCCCCCCEEEECCC
TIPDHDILVAGFPCQPFSIAGVSKKISLGRKTGFEDKTQGTLFFDVCRILKEKRPKAFML
CCCCCCEEEECCCCCCCEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ENVKNLKSHDKGRTFKTILESLDELKYKVFFAVLDGQNFVPQHRERIIIVGFDMERYGDD
HHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECHHHHCCC
IEFDFDITPVNPKPVMRDILEKKVDDKYTLSDNLWTYLQNYAAKHRAAGNGFGYGIAPLD
EEEEEEECCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVSRTISARYHKDGSEILIAQKGKNPRRLTPRECARLQGFPESFVIPVSDTQAYRQFGNS
CHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHCCC
VVVPLIENVAKLIVEKIDLLESDVQNEKRKAI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWYIFFPGGNYMKQMHLRVDDALYEELNTYSVETEQSMQACVREAVVYYITDKKKKQIPI
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCC
LKSQFTFIDLFAGIGGMRLAYENVGGRCVYSNEWNKYSQQTYYANFGEQPEGDITKVDAK
HHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHCCEEEECCCCCCCCCEEEECCC
TIPDHDILVAGFPCQPFSIAGVSKKISLGRKTGFEDKTQGTLFFDVCRILKEKRPKAFML
CCCCCCEEEECCCCCCCEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ENVKNLKSHDKGRTFKTILESLDELKYKVFFAVLDGQNFVPQHRERIIIVGFDMERYGDD
HHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECHHHHCCC
IEFDFDITPVNPKPVMRDILEKKVDDKYTLSDNLWTYLQNYAAKHRAAGNGFGYGIAPLD
EEEEEEECCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVSRTISARYHKDGSEILIAQKGKNPRRLTPRECARLQGFPESFVIPVSDTQAYRQFGNS
CHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHCCC
VVVPLIENVAKLIVEKIDLLESDVQNEKRKAI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8481004 [H]