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The map label for this gene is ecoRIIR [H]

Identifier: 238922590

GI number: 238922590

Start: 157920

End: 159095

Strand: Direct

Name: ecoRIIR [H]

Synonym: EUBREC_0164

Alternate gene names: 238922590

Gene position: 157920-159095 (Clockwise)

Preceding gene: 238922589

Following gene: 238922591

Centisome position: 4.58

GC content: 34.69

Gene sequence:

>1176_bases
ATGTCAACAGAAATACTAAACAAAGCAATAGAATCAACTGTTGCAAGTGAATTGGCTTTTTGCAAATTTTTATCTGCGAA
TGATACTGGAGCGACAGGTGGACATCAAGGCGGAGTTCTTGTGTCAGTATCTGCAAGTCGCATGTTATTTGTAGAAATAT
TGCCAGATAATGATATTTTAAAGAGAGATGTTAAGATAACATGGCAAGGAGATTTGGTTACAGAAAGTACATTTACTTAT
TACAGTAGCAAAAAGGAACTGAGAATTACGAAATTTGGAAGAGACTTTGATATAATTAATCCGGATAGAACAGGTTCTTT
ATTTGTCTTGACAAAACAAAGTTGGGATGATTATTCCGTATTTATAATGGATACGGAGGATGAAATAGAAGAATTTTTGA
GTACATTTGGGATAAGTGCAACAGAGACTAATTGTTTGTTTGGAGCCGGAGGCGTACAAAGAAGTGTTATAGAACAGCAG
GCTATCGAAACATTTATTTCCTCGTTGGAAGTAGAATTTCCAGAGACAGAGGTGATGTCATCGGCTGCAAGAAATATTTC
TGATGCAGTGTATAATCATGTTGAGTATTTGATTACAAATCCTGATAAAAAGATTATAGAATGGACTAATATGGAATATG
CATTATTTAGAGCGCTGGAAGAATATAGATACGGAGATATTGTAAGATGTGGATTTCCGTCAGTTGAAGAATTTGTTAGT
GTTGCGAATTCTGTATTAAATAGACGTAAGAGCAGAGCAGGAAAGAGTTTAGAACATCATTTGGAGGCAATATTTGTAGC
TAATGAAATTATTTATGATGCGCAACCTGTTACAGAAGGAAAAAAGAAACCAGATTTTCTATTTCCATCAGCAGTGGCAT
ATCGTGATTTGACATATCCTGTAAGTAAATTGGCGACCTTGGCAGCTAAAACAACTTGTAAGGATAGATGGCGTCAAATT
CTGAATGAGGCAAATCGATTAAAAGATGAAAATAAGTTTTTATGCACATTGCAACAGGGAGTATCACCTATGCAAATGGA
TGAGATGGAGGCAGAAAAAGTTATTCTTGTGGTACCTAAACCATACATATCATGTTATCCAAGAGATAGACAAGATAGAA
TTTGGACAATATCTCGTTTTGTAAGGTATATTAAGAGTATTCAAAATACTGATTAA

Upstream 100 bases:

>100_bases
CGGTAGTTGTTCCATTAATAGAGAATGTTGCAAAACTGATTGTAGAAAAAATTGATTTATTAGAATCGGATGTTCAGAAT
GAAAAAAGGAAGGCGATTTA

Downstream 100 bases:

>100_bases
GTCTCTACAGTGTTAATAATATGCGGATTTTTAATAACACCAAATTATGGTGAATTTTACTAAAAAAACACCAACCCTGT
CCATAGACAAACACAAAACC

Product: putative type II restriction enzyme

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSTEILNKAIESTVASELAFCKFLSANDTGATGGHQGGVLVSVSASRMLFVEILPDNDILKRDVKITWQGDLVTESTFTY
YSSKKELRITKFGRDFDIINPDRTGSLFVLTKQSWDDYSVFIMDTEDEIEEFLSTFGISATETNCLFGAGGVQRSVIEQQ
AIETFISSLEVEFPETEVMSSAARNISDAVYNHVEYLITNPDKKIIEWTNMEYALFRALEEYRYGDIVRCGFPSVEEFVS
VANSVLNRRKSRAGKSLEHHLEAIFVANEIIYDAQPVTEGKKKPDFLFPSAVAYRDLTYPVSKLATLAAKTTCKDRWRQI
LNEANRLKDENKFLCTLQQGVSPMQMDEMEAEKVILVVPKPYISCYPRDRQDRIWTISRFVRYIKSIQNTD

Sequences:

>Translated_391_residues
MSTEILNKAIESTVASELAFCKFLSANDTGATGGHQGGVLVSVSASRMLFVEILPDNDILKRDVKITWQGDLVTESTFTY
YSSKKELRITKFGRDFDIINPDRTGSLFVLTKQSWDDYSVFIMDTEDEIEEFLSTFGISATETNCLFGAGGVQRSVIEQQ
AIETFISSLEVEFPETEVMSSAARNISDAVYNHVEYLITNPDKKIIEWTNMEYALFRALEEYRYGDIVRCGFPSVEEFVS
VANSVLNRRKSRAGKSLEHHLEAIFVANEIIYDAQPVTEGKKKPDFLFPSAVAYRDLTYPVSKLATLAAKTTCKDRWRQI
LNEANRLKDENKFLCTLQQGVSPMQMDEMEAEKVILVVPKPYISCYPRDRQDRIWTISRFVRYIKSIQNTD
>Mature_390_residues
STEILNKAIESTVASELAFCKFLSANDTGATGGHQGGVLVSVSASRMLFVEILPDNDILKRDVKITWQGDLVTESTFTYY
SSKKELRITKFGRDFDIINPDRTGSLFVLTKQSWDDYSVFIMDTEDEIEEFLSTFGISATETNCLFGAGGVQRSVIEQQA
IETFISSLEVEFPETEVMSSAARNISDAVYNHVEYLITNPDKKIIEWTNMEYALFRALEEYRYGDIVRCGFPSVEEFVSV
ANSVLNRRKSRAGKSLEHHLEAIFVANEIIYDAQPVTEGKKKPDFLFPSAVAYRDLTYPVSKLATLAAKTTCKDRWRQIL
NEANRLKDENKFLCTLQQGVSPMQMDEMEAEKVILVVPKPYISCYPRDRQDRIWTISRFVRYIKSIQNTD

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 44475; Mature: 44344

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEILNKAIESTVASELAFCKFLSANDTGATGGHQGGVLVSVSASRMLFVEILPDNDIL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCE
KRDVKITWQGDLVTESTFTYYSSKKELRITKFGRDFDIINPDRTGSLFVLTKQSWDDYSV
ECCEEEEECCCEEECCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEE
FIMDTEDEIEEFLSTFGISATETNCLFGAGGVQRSVIEQQAIETFISSLEVEFPETEVMS
EEECCHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SAARNISDAVYNHVEYLITNPDKKIIEWTNMEYALFRALEEYRYGDIVRCGFPSVEEFVS
HHHHHHHHHHHHHHHEEEECCCHHEEEECCCHHHHHHHHHHCCCCCEEECCCCCHHHHHH
VANSVLNRRKSRAGKSLEHHLEAIFVANEIIYDAQPVTEGKKKPDFLFPSAVAYRDLTYP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
VSKLATLAAKTTCKDRWRQILNEANRLKDENKFLCTLQQGVSPMQMDEMEAEKVILVVPK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCHHHHHCCCEEEEEECC
PYISCYPRDRQDRIWTISRFVRYIKSIQNTD
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STEILNKAIESTVASELAFCKFLSANDTGATGGHQGGVLVSVSASRMLFVEILPDNDIL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCE
KRDVKITWQGDLVTESTFTYYSSKKELRITKFGRDFDIINPDRTGSLFVLTKQSWDDYSV
ECCEEEEECCCEEECCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEE
FIMDTEDEIEEFLSTFGISATETNCLFGAGGVQRSVIEQQAIETFISSLEVEFPETEVMS
EEECCHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SAARNISDAVYNHVEYLITNPDKKIIEWTNMEYALFRALEEYRYGDIVRCGFPSVEEFVS
HHHHHHHHHHHHHHHEEEECCCHHEEEECCCHHHHHHHHHHCCCCCEEECCCCCHHHHHH
VANSVLNRRKSRAGKSLEHHLEAIFVANEIIYDAQPVTEGKKKPDFLFPSAVAYRDLTYP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
VSKLATLAAKTTCKDRWRQILNEANRLKDENKFLCTLQQGVSPMQMDEMEAEKVILVVPK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCHHHHHCCCEEEEEECC
PYISCYPRDRQDRIWTISRFVRYIKSIQNTD
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]