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The map label for this gene is spaR [H]

Identifier: 238922516

GI number: 238922516

Start: 86577

End: 87239

Strand: Direct

Name: spaR [H]

Synonym: EUBREC_0090

Alternate gene names: 238922516

Gene position: 86577-87239 (Clockwise)

Preceding gene: 238922515

Following gene: 238922517

Centisome position: 2.51

GC content: 38.46

Gene sequence:

>663_bases
ATGGCAAAAATACTGGCAGTTGATGATGAACCGGCAATTCTGGAAATGATAGAAAGCATTTTGAATAAGGATGGACATCT
GGTTACCAAAGTAAGTAATCCACTAAAAATTAATATGGAAGAATTACATCGTTATGACCTGATTTTACTGGACATTATGA
TGCCTGGAATTGATGGCTTCGAATTATGCAAAAGAATCAGGGCACTTGTGGATTGTCCGATTTTGTTTCTTACGGCAAAA
ACGGAGGAAAACAGTCTGGTAAACGGACTTTCTTTAGGAGCAGATGATTATATTTCAAAGCCATTTGGAGTGATGGAACT
TCGGGCAAGGATCAATGCACATCTTAGAAGAGAGCACAGGGAACATTCTGTCCGGATGGTTTTGGGGAGGGTCTGTATTC
AAATATCTCAAAAGAAACTATTGGTTGATGATAAGGAACTTCCTCTTACGAAAGCGGAGTACGAAATCTGTGAATTTCTG
GCTAAAAACAGAGGACAGGTTTTCTCGAAGGAACAGATATTGGAAGCGGTCTTTGGCTTTGACAATGAGAGTAATGACAG
TACTATAATCACGCATATCAAAAATATAAGAGCAAAATTTGCGGATTATGATTATACACCGGTAAAAACAGTCTGGGGGA
TTGGATATAAATGGGAAGAGTAA

Upstream 100 bases:

>100_bases
CAGGTTGCTTAATATTTACAGGGATTAGTATGGTATACTATTTTTGGTGGGTAAACCACTGGGAAGGAAACAGAATATCG
GAATAGAATCGAGGGAAACT

Downstream 100 bases:

>100_bases
AGAGAAGCAATGCACTCAGCAGGATTTTTATGAGATATGTACTGGTCATGCTTGGATCATTAGTTGGTTTGGTGATTGTT
GCTTATCTGCTGCTGTACCT

Product: hypothetical protein

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKILAVDDEPAILEMIESILNKDGHLVTKVSNPLKINMEELHRYDLILLDIMMPGIDGFELCKRIRALVDCPILFLTAK
TEENSLVNGLSLGADDYISKPFGVMELRARINAHLRREHREHSVRMVLGRVCIQISQKKLLVDDKELPLTKAEYEICEFL
AKNRGQVFSKEQILEAVFGFDNESNDSTIITHIKNIRAKFADYDYTPVKTVWGIGYKWEE

Sequences:

>Translated_220_residues
MAKILAVDDEPAILEMIESILNKDGHLVTKVSNPLKINMEELHRYDLILLDIMMPGIDGFELCKRIRALVDCPILFLTAK
TEENSLVNGLSLGADDYISKPFGVMELRARINAHLRREHREHSVRMVLGRVCIQISQKKLLVDDKELPLTKAEYEICEFL
AKNRGQVFSKEQILEAVFGFDNESNDSTIITHIKNIRAKFADYDYTPVKTVWGIGYKWEE
>Mature_219_residues
AKILAVDDEPAILEMIESILNKDGHLVTKVSNPLKINMEELHRYDLILLDIMMPGIDGFELCKRIRALVDCPILFLTAKT
EENSLVNGLSLGADDYISKPFGVMELRARINAHLRREHREHSVRMVLGRVCIQISQKKLLVDDKELPLTKAEYEICEFLA
KNRGQVFSKEQILEAVFGFDNESNDSTIITHIKNIRAKFADYDYTPVKTVWGIGYKWEE

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=230, Percent_Identity=35.2173913043478, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=31.1111111111111, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1790860, Length=219, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=30.4545454545455, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=216, Percent_Identity=31.0185185185185, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=31.8385650224215, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=31.6742081447964, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=32.4324324324324, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=30.3964757709251, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=34.5132743362832, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=31.2217194570136, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=28.5087719298246, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787229, Length=208, Percent_Identity=27.8846153846154, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI145693140, Length=241, Percent_Identity=29.045643153527, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25221; Mature: 25089

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILAVDDEPAILEMIESILNKDGHLVTKVSNPLKINMEELHRYDLILLDIMMPGIDGF
CCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHEEHHCCCCCHH
ELCKRIRALVDCPILFLTAKTEENSLVNGLSLGADDYISKPFGVMELRARINAHLRREHR
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
EHSVRMVLGRVCIQISQKKLLVDDKELPLTKAEYEICEFLAKNRGQVFSKEQILEAVFGF
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DNESNDSTIITHIKNIRAKFADYDYTPVKTVWGIGYKWEE
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEECC
>Mature Secondary Structure 
AKILAVDDEPAILEMIESILNKDGHLVTKVSNPLKINMEELHRYDLILLDIMMPGIDGF
CEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHEEHHCCCCCHH
ELCKRIRALVDCPILFLTAKTEENSLVNGLSLGADDYISKPFGVMELRARINAHLRREHR
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
EHSVRMVLGRVCIQISQKKLLVDDKELPLTKAEYEICEFLAKNRGQVFSKEQILEAVFGF
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DNESNDSTIITHIKNIRAKFADYDYTPVKTVWGIGYKWEE
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]