| Definition | Eubacterium rectale ATCC 33656, complete genome. |
|---|---|
| Accession | NC_012781 |
| Length | 3,449,685 |
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The map label for this gene is spaK [H]
Identifier: 238922517
GI number: 238922517
Start: 87227
End: 88603
Strand: Direct
Name: spaK [H]
Synonym: EUBREC_0091
Alternate gene names: 238922517
Gene position: 87227-88603 (Clockwise)
Preceding gene: 238922516
Following gene: 238922518
Centisome position: 2.53
GC content: 38.71
Gene sequence:
>1377_bases ATGGGAAGAGTAAAGAGAAGCAATGCACTCAGCAGGATTTTTATGAGATATGTACTGGTCATGCTTGGATCATTAGTTGG TTTGGTGATTGTTGCTTATCTGCTGCTGTACCTTTTGATTAGTGTGGGCTGTATTTATCCGGCAAATTATGCAGAGCAAA AGATTAATGAAGCATATGATACGATTCTACGTGCAGATAAAGTGACTGCTGAGATGATTCCCGCACTTTGTGATTATGTA ATCTTTTCAGAGAATGGAGAGAAAATAGGTGGAGATCTGTCAGAACAATACGAACAGATAGCATGGAATGTTGCAAAGCA CGGAAACGCATCCGGGAAATATTTTTATAAAGTAATTGTAAGGGAAAATGAATATGTTGTATTGCAATATCGCCTGACAC CTCAATATCATTCAGCTTTTTTGAGGGAGCATTTTATAGGACCACAGAATGTGATGATTATTATGTCTGTGATTGGAGCG GTAGCGATTATCATCATTCAGTCCATACGTTTTGGAAAAAGAATCAAAAAGCAGATGCAGCCTGTATTAGACGCAATCGG ACAAATAAAGGATCAAAATCTGGAATATGAGACATCCTGTTCCGGTATCAAAGAGTTTGATGACTGTTTATCGGCAATCG ATGATATGCGAGATGCATTGCGAGAATCTTTAGAAAAGCAGTGGAAAACAGAGCATGAAAAGAAACAGCAGATGTCTGCT TTGGCGCATGATATTAAAACACCTCTTACGATTGTACGGGGAAATGCAGAACTACTTTCAGAGACTGAACTGACCACAGA ACAGAAGAAAAATATCACTTATGTTTTGAACGGCACAACACAGATACAAAGTTATGTAAAACAGTTGATAGATGTCACAA AATCATGGAATTGCAGTGATGTTACCTATACAACGGTGAGGTTAGAAGATTTTTTCGCAGATATAAAGGAACAGGCACTC GGACTGGTAGAAATCTATCACCAGAAGATAGATTGGAAGGCGGAACAAAGTGATAAAAAGGTAACGATTGCTTATGATCC AATGTTCCGGGCCGTAATGAATATGATTCAGAATGCAGTGGAGCATACAAAAGAAAATGGAATCATTTATATTGACGCAA AGGAGCAGGATGGCCGGCTGACATTCATTGTGGAGGATAGCGGATCAGGATTTACAAAAGAAGCATTATTGCATGGCACA GAGCAGTTTTTTATGGATGACACAAGTAGAAATGGCGAAGCCCATTATGGGATGGGACTATTTTTTGCAAAAACTGTGGC TGAAAAATATGGTGGAGGTATTAAACTTTCAAATTCTGAAAATACAGGTGGGGCGAGGGTAGAAATATTTTTCCTGAGTA GTCAAGAAACAAGCTAA
Upstream 100 bases:
>100_bases GTAATGACAGTACTATAATCACGCATATCAAAAATATAAGAGCAAAATTTGCGGATTATGATTATACACCGGTAAAAACA GTCTGGGGGATTGGATATAA
Downstream 100 bases:
>100_bases ATAGACCGCAATTAAGCTATGTAAAAGAAATTTTACATGCTTTTATCTAATATGTAACGAGAAAAATGAGCTTCATAATC GAAATCTTCATATAAGTGGA
Product: subtilin biosynthesis sensor protein SpaK
Products: NA
Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]
Number of amino acids: Translated: 458; Mature: 457
Protein sequence:
>458_residues MGRVKRSNALSRIFMRYVLVMLGSLVGLVIVAYLLLYLLISVGCIYPANYAEQKINEAYDTILRADKVTAEMIPALCDYV IFSENGEKIGGDLSEQYEQIAWNVAKHGNASGKYFYKVIVRENEYVVLQYRLTPQYHSAFLREHFIGPQNVMIIMSVIGA VAIIIIQSIRFGKRIKKQMQPVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEHEKKQQMSA LAHDIKTPLTIVRGNAELLSETELTTEQKKNITYVLNGTTQIQSYVKQLIDVTKSWNCSDVTYTTVRLEDFFADIKEQAL GLVEIYHQKIDWKAEQSDKKVTIAYDPMFRAVMNMIQNAVEHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGT EQFFMDDTSRNGEAHYGMGLFFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS
Sequences:
>Translated_458_residues MGRVKRSNALSRIFMRYVLVMLGSLVGLVIVAYLLLYLLISVGCIYPANYAEQKINEAYDTILRADKVTAEMIPALCDYV IFSENGEKIGGDLSEQYEQIAWNVAKHGNASGKYFYKVIVRENEYVVLQYRLTPQYHSAFLREHFIGPQNVMIIMSVIGA VAIIIIQSIRFGKRIKKQMQPVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEHEKKQQMSA LAHDIKTPLTIVRGNAELLSETELTTEQKKNITYVLNGTTQIQSYVKQLIDVTKSWNCSDVTYTTVRLEDFFADIKEQAL GLVEIYHQKIDWKAEQSDKKVTIAYDPMFRAVMNMIQNAVEHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGT EQFFMDDTSRNGEAHYGMGLFFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS >Mature_457_residues GRVKRSNALSRIFMRYVLVMLGSLVGLVIVAYLLLYLLISVGCIYPANYAEQKINEAYDTILRADKVTAEMIPALCDYVI FSENGEKIGGDLSEQYEQIAWNVAKHGNASGKYFYKVIVRENEYVVLQYRLTPQYHSAFLREHFIGPQNVMIIMSVIGAV AIIIIQSIRFGKRIKKQMQPVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEHEKKQQMSAL AHDIKTPLTIVRGNAELLSETELTTEQKKNITYVLNGTTQIQSYVKQLIDVTKSWNCSDVTYTTVRLEDFFADIKEQALG LVEIYHQKIDWKAEQSDKKVTIAYDPMFRAVMNMIQNAVEHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGTE QFFMDDTSRNGEAHYGMGLFFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS
Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]
COG id: COG0642
COG function: function code T; Signal transduction histidine kinase
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 histidine kinase domain [H]
Homologues:
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=27.6785714285714, Blast_Score=87, Evalue=2e-18, Organism=Escherichia coli, GI87082128, Length=213, Percent_Identity=24.8826291079812, Blast_Score=64, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003594 - InterPro: IPR008358 - InterPro: IPR003661 - InterPro: IPR005467 - InterPro: IPR009082 [H]
Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]
EC number: =2.7.13.3 [H]
Molecular weight: Translated: 51916; Mature: 51785
Theoretical pI: Translated: 5.53; Mature: 5.53
Prosite motif: PS50109 HIS_KIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGRVKRSNALSRIFMRYVLVMLGSLVGLVIVAYLLLYLLISVGCIYPANYAEQKINEAYD CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH TILRADKVTAEMIPALCDYVIFSENGEKIGGDLSEQYEQIAWNVAKHGNASGKYFYKVIV HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE RENEYVVLQYRLTPQYHSAFLREHFIGPQNVMIIMSVIGAVAIIIIQSIRFGKRIKKQMQ ECCCEEEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH PVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEHEKKQQMSA HHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LAHDIKTPLTIVRGNAELLSETELTTEQKKNITYVLNGTTQIQSYVKQLIDVTKSWNCSD HHHHHCCCEEEEECCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC VTYTTVRLEDFFADIKEQALGLVEIYHQKIDWKAEQSDKKVTIAYDPMFRAVMNMIQNAV CEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH EHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGTEQFFMDDTSRNGEAHYGMGL HHHHCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCHHH FFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS HHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCC >Mature Secondary Structure GRVKRSNALSRIFMRYVLVMLGSLVGLVIVAYLLLYLLISVGCIYPANYAEQKINEAYD CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH TILRADKVTAEMIPALCDYVIFSENGEKIGGDLSEQYEQIAWNVAKHGNASGKYFYKVIV HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE RENEYVVLQYRLTPQYHSAFLREHFIGPQNVMIIMSVIGAVAIIIIQSIRFGKRIKKQMQ ECCCEEEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH PVLDAIGQIKDQNLEYETSCSGIKEFDDCLSAIDDMRDALRESLEKQWKTEHEKKQQMSA HHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LAHDIKTPLTIVRGNAELLSETELTTEQKKNITYVLNGTTQIQSYVKQLIDVTKSWNCSD HHHHHCCCEEEEECCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC VTYTTVRLEDFFADIKEQALGLVEIYHQKIDWKAEQSDKKVTIAYDPMFRAVMNMIQNAV CEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH EHTKENGIIYIDAKEQDGRLTFIVEDSGSGFTKEALLHGTEQFFMDDTSRNGEAHYGMGL HHHHCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCHHH FFAKTVAEKYGGGIKLSNSENTGGARVEIFFLSSQETS HHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8439156; 8085823 [H]