| Definition | Eubacterium rectale ATCC 33656, complete genome. |
|---|---|
| Accession | NC_012781 |
| Length | 3,449,685 |
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The map label for this gene is metG [H]
Identifier: 238922506
GI number: 238922506
Start: 80417
End: 80875
Strand: Direct
Name: metG [H]
Synonym: EUBREC_0080
Alternate gene names: 238922506
Gene position: 80417-80875 (Clockwise)
Preceding gene: 238922505
Following gene: 238922507
Centisome position: 2.33
GC content: 41.83
Gene sequence:
>459_bases ATGAAGAGCATTGGTGGCGTAAAGGATGAAAATGGCGTAAGTTCTAAGCCTTCAGAAGAGGTAAAAGCTGAGCCGGAGAA GATTGATTTCTCTAATGTAAAGATTGAGCCTTTATTTGAGGAAGAAGTAGACTTCGATACATTCAGCAAGTCAGATTTCC GTGCAGTAAAGGTTAAAGAGTGTGTTGCAGTACCGAAGTCAAAGAAACTGTTACAGTTTACTCTTGATGACGGAACAGGC ACAGACAGAACCATTTTAAGCGGTATTCATAGCTTTTATGAGCCGGAAGAACTGGTTGGAAAGACTCTTATTGCTATCAC AAATCTTCCACCGAGAGCTATGATGGGCATTGACTCTTGCGGTATGCTCCTCAGTGCTATTCATGAAGAAGAAGGCGAAG AAAAGCTCCATCTTCTGATGGTTGATGACCATATTCCAGCAGGTGCAAAGCTCTATTAA
Upstream 100 bases:
>100_bases TCCAAGAGTGACCAAGTATATAGAATCCGGGAAAGCCTATTTTCCAAGGGTTTGCAAGGATATACAAGAGTTAGATTAGC AATCAAAAAAATTCCCGACA
Downstream 100 bases:
>100_bases GATGATGTAAAGATGTACCGTTTTACCGAATAGACGGGACGTACTTCATTTGATAAAAAACTAAAAAAACAGCGATTTAC ATCAAACTTACATCAATTGA
Product: methionyl-tRNA synthetase
Products: NA
Alternate protein names: Methionine--tRNA ligase; MetRS [H]
Number of amino acids: Translated: 152; Mature: 152
Protein sequence:
>152_residues MKSIGGVKDENGVSSKPSEEVKAEPEKIDFSNVKIEPLFEEEVDFDTFSKSDFRAVKVKECVAVPKSKKLLQFTLDDGTG TDRTILSGIHSFYEPEELVGKTLIAITNLPPRAMMGIDSCGMLLSAIHEEEGEEKLHLLMVDDHIPAGAKLY
Sequences:
>Translated_152_residues MKSIGGVKDENGVSSKPSEEVKAEPEKIDFSNVKIEPLFEEEVDFDTFSKSDFRAVKVKECVAVPKSKKLLQFTLDDGTG TDRTILSGIHSFYEPEELVGKTLIAITNLPPRAMMGIDSCGMLLSAIHEEEGEEKLHLLMVDDHIPAGAKLY >Mature_152_residues MKSIGGVKDENGVSSKPSEEVKAEPEKIDFSNVKIEPLFEEEVDFDTFSKSDFRAVKVKECVAVPKSKKLLQFTLDDGTG TDRTILSGIHSFYEPEELVGKTLIAITNLPPRAMMGIDSCGMLLSAIHEEEGEEKLHLLMVDDHIPAGAKLY
Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]
COG id: COG0073
COG function: function code R; EMAP domain
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 tRNA-binding domain [H]
Homologues:
Organism=Escherichia coli, GI1788432, Length=133, Percent_Identity=37.593984962406, Blast_Score=64, Evalue=4e-12,
Paralogues:
None
Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001412 - InterPro: IPR015413 - InterPro: IPR004495 - InterPro: IPR014758 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR014729 - InterPro: IPR002547 - InterPro: IPR009080 - InterPro: IPR013155 [H]
Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]
EC number: =6.1.1.10 [H]
Molecular weight: Translated: 16769; Mature: 16769
Theoretical pI: Translated: 4.53; Mature: 4.53
Prosite motif: PS50886 TRBD
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSIGGVKDENGVSSKPSEEVKAEPEKIDFSNVKIEPLFEEEVDFDTFSKSDFRAVKVKE CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEHHHH CVAVPKSKKLLQFTLDDGTGTDRTILSGIHSFYEPEELVGKTLIAITNLPPRAMMGIDSC HHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHEEEEEECCCCHHHHCCCHH GMLLSAIHEEEGEEKLHLLMVDDHIPAGAKLY HHHHHHHHHCCCCCEEEEEEEECCCCCCCCCC >Mature Secondary Structure MKSIGGVKDENGVSSKPSEEVKAEPEKIDFSNVKIEPLFEEEVDFDTFSKSDFRAVKVKE CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEHHHH CVAVPKSKKLLQFTLDDGTGTDRTILSGIHSFYEPEELVGKTLIAITNLPPRAMMGIDSC HHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHEEEEEECCCCHHHHCCCHH GMLLSAIHEEEGEEKLHLLMVDDHIPAGAKLY HHHHHHHHHCCCCCEEEEEEEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA