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The map label for this gene is lysS [H]

Identifier: 238922489

GI number: 238922489

Start: 66915

End: 68528

Strand: Direct

Name: lysS [H]

Synonym: EUBREC_0063

Alternate gene names: 238922489

Gene position: 66915-68528 (Clockwise)

Preceding gene: 238922488

Following gene: 238922490

Centisome position: 1.94

GC content: 46.53

Gene sequence:

>1614_bases
ATGGCAAATCAGAATAATAACAAGGGTGGACAGCAGCAGGACGTAAACCAGCTCCTTAAGGTCCGCCGTGAAAAATTACA
GAATTTACAGGAAGCAGGAAAGGATCCCTTCCAGATTACAAAGTACAATGTCACTCATCACAGCAGCGATGTAAAGGAGC
TTTACAATGCGCATGAGGCTGAGATTCTCGGTGACAGAAAGGCTCCGGATGTTGAGGGCTTGGATGACGCTGCAAAGCGA
GAGGTAATCAACAATGATTACAACGAGAGAAGAGAAATCATGGATGCAAAGCCAATCGAGGTAAGCATTGCCGGACGTAT
GATGTTCAAGCGTGTCATGGGTAAGGCTTCTTTCTGCAATATTCAGGACTTAAAGGGCAATATCCAGGTATATGTGGCAA
GGGACAATGTCGGAGAAGACTCATACGCAGACTTCAAGAAATCAGACATCGGTGATATCTACGGTGTAAAGGGCTTCGCT
TTCAGAACAAAGACAGGCGAAATCTCAATCCATGCAGAGGAGATAACACTCCTTTCAAAATCACTCCAGATACTCCCGGA
GAAGTTCCATGGACTGACAGACACAGACACCAGATATCGTCAGAGATATGTAGACCTTATCATGAATCAGGAGAGCAAAG
AGGTATTTATCAAGCGCTCACAGATACTCAAGGAAATCCGTAACTTCCTTGCAGGCCGTGATTTCATGGAGGTTGAGACA
CCTATGCTTGTTTCAAACGCAGGTGGTGCCGCAGCAAGACCATTCGAGACACACTACAACGCATTGAACGAGGATGTAAA
GCTTCGTATTTCCCTTGAGCTTTACTTAAAGAGACTCATCGTCGGAGGACTTGAGCGAGTATACGAGATCGGACGAGTTT
TCCGTAACGAGGGCGTAGATACACGTCACAACCCTGAGTTCACACTTATGGAACTTTATCAGGCATACACAGACTACGAG
GGCATGATGGAGCTGACAGAGTCAATGTTCCGTTACCTCGCTGAGAAGGTATGCGGTTCGACAAAAATCTCATACAACGG
CGTGGAAATCGACCTTGGAAAGCCATTCGCAAGAATGACAATGATAGACGCAATCAAGAAATACGCAGGCGTAGACTTCG
ACCAGGTACCTGATGATGCAGCCGCAAAGAAGCTTGCAGACGAGCATCATATCGAGTATGAGGAGCGTCACAAGAAGGGT
GACATCGTAAACCTTTTCTTCGAGGAGTACTGTGAGAAAGAGCTTATCCAGCCAACATTCATCATGGATCACCCAATTGA
GATATCACCTCTCACAAAGAAGAAACCAAGCGATCCTACAAAGGTAGAGCGTTTTGAGCTGTTCTGTAATACATGGGAGA
TGTGTAACGCATACTCAGAGCTCAACGACCCAATCGACCAGCGTGAGCGTTTCGCAGCACAGGATGCCAACGCAGCAGCA
GGCGATGACGAGGCAGAGCACACAGATGAGGACTTCCTCAATGCACTTGAAATCGGTATGCCGCCAACAGGTGGAATCGG
ATACGGAATCGATCGTCTTGTAATGCTTTTGACAGATAGCCAGGCAATTAGGGACGTATTACTGTTCCCGACAATGAAGA
GCTTGGATAAATAA

Upstream 100 bases:

>100_bases
GAAAGTCGGAGATACTGTAACTGTTGAGACTCAGGTAGGAGAGCTCACATACAAGGTACTCGAGATCCAGAGAGCAGAAG
AGAACTAAAAGAGGTAAAAA

Downstream 100 bases:

>100_bases
ACCTAGTGTTTATGCGGGTTTGAGGCATTTGAGTACGTCAAAGGACGCATTAAAAGACGTATTTTGGCATAGTCATTTAC
ATCAGTATGAAAGAGTACAC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MANQNNNKGGQQQDVNQLLKVRREKLQNLQEAGKDPFQITKYNVTHHSSDVKELYNAHEAEILGDRKAPDVEGLDDAAKR
EVINNDYNERREIMDAKPIEVSIAGRMMFKRVMGKASFCNIQDLKGNIQVYVARDNVGEDSYADFKKSDIGDIYGVKGFA
FRTKTGEISIHAEEITLLSKSLQILPEKFHGLTDTDTRYRQRYVDLIMNQESKEVFIKRSQILKEIRNFLAGRDFMEVET
PMLVSNAGGAAARPFETHYNALNEDVKLRISLELYLKRLIVGGLERVYEIGRVFRNEGVDTRHNPEFTLMELYQAYTDYE
GMMELTESMFRYLAEKVCGSTKISYNGVEIDLGKPFARMTMIDAIKKYAGVDFDQVPDDAAAKKLADEHHIEYEERHKKG
DIVNLFFEEYCEKELIQPTFIMDHPIEISPLTKKKPSDPTKVERFELFCNTWEMCNAYSELNDPIDQRERFAAQDANAAA
GDDEAEHTDEDFLNALEIGMPPTGGIGYGIDRLVMLLTDSQAIRDVLLFPTMKSLDK

Sequences:

>Translated_537_residues
MANQNNNKGGQQQDVNQLLKVRREKLQNLQEAGKDPFQITKYNVTHHSSDVKELYNAHEAEILGDRKAPDVEGLDDAAKR
EVINNDYNERREIMDAKPIEVSIAGRMMFKRVMGKASFCNIQDLKGNIQVYVARDNVGEDSYADFKKSDIGDIYGVKGFA
FRTKTGEISIHAEEITLLSKSLQILPEKFHGLTDTDTRYRQRYVDLIMNQESKEVFIKRSQILKEIRNFLAGRDFMEVET
PMLVSNAGGAAARPFETHYNALNEDVKLRISLELYLKRLIVGGLERVYEIGRVFRNEGVDTRHNPEFTLMELYQAYTDYE
GMMELTESMFRYLAEKVCGSTKISYNGVEIDLGKPFARMTMIDAIKKYAGVDFDQVPDDAAAKKLADEHHIEYEERHKKG
DIVNLFFEEYCEKELIQPTFIMDHPIEISPLTKKKPSDPTKVERFELFCNTWEMCNAYSELNDPIDQRERFAAQDANAAA
GDDEAEHTDEDFLNALEIGMPPTGGIGYGIDRLVMLLTDSQAIRDVLLFPTMKSLDK
>Mature_536_residues
ANQNNNKGGQQQDVNQLLKVRREKLQNLQEAGKDPFQITKYNVTHHSSDVKELYNAHEAEILGDRKAPDVEGLDDAAKRE
VINNDYNERREIMDAKPIEVSIAGRMMFKRVMGKASFCNIQDLKGNIQVYVARDNVGEDSYADFKKSDIGDIYGVKGFAF
RTKTGEISIHAEEITLLSKSLQILPEKFHGLTDTDTRYRQRYVDLIMNQESKEVFIKRSQILKEIRNFLAGRDFMEVETP
MLVSNAGGAAARPFETHYNALNEDVKLRISLELYLKRLIVGGLERVYEIGRVFRNEGVDTRHNPEFTLMELYQAYTDYEG
MMELTESMFRYLAEKVCGSTKISYNGVEIDLGKPFARMTMIDAIKKYAGVDFDQVPDDAAAKKLADEHHIEYEERHKKGD
IVNLFFEEYCEKELIQPTFIMDHPIEISPLTKKKPSDPTKVERFELFCNTWEMCNAYSELNDPIDQRERFAAQDANAAAG
DDEAEHTDEDFLNALEIGMPPTGGIGYGIDRLVMLLTDSQAIRDVLLFPTMKSLDK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=551, Percent_Identity=39.7459165154265, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI5031815, Length=551, Percent_Identity=39.7459165154265, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI45439306, Length=469, Percent_Identity=23.6673773987207, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI40789249, Length=299, Percent_Identity=24.4147157190635, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1789256, Length=537, Percent_Identity=49.9068901303538, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1790571, Length=535, Percent_Identity=49.1588785046729, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=29.2834890965732, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1788173, Length=281, Percent_Identity=25.6227758007117, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=456, Percent_Identity=43.421052631579, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17535927, Length=456, Percent_Identity=43.421052631579, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=456, Percent_Identity=43.421052631579, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI32566633, Length=309, Percent_Identity=26.8608414239482, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=495, Percent_Identity=23.4343434343434, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=458, Percent_Identity=45.4148471615721, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324256, Length=504, Percent_Identity=30.952380952381, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=391, Percent_Identity=26.3427109974425, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325153, Length=170, Percent_Identity=31.7647058823529, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321807, Length=447, Percent_Identity=24.3847874720358, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640849, Length=456, Percent_Identity=44.7368421052632, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640851, Length=456, Percent_Identity=44.7368421052632, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24584738, Length=301, Percent_Identity=26.2458471760797, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136276, Length=465, Percent_Identity=22.3655913978495, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 61422; Mature: 61291

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQNNNKGGQQQDVNQLLKVRREKLQNLQEAGKDPFQITKYNVTHHSSDVKELYNAHEA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
EILGDRKAPDVEGLDDAAKREVINNDYNERREIMDAKPIEVSIAGRMMFKRVMGKASFCN
HHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCC
IQDLKGNIQVYVARDNVGEDSYADFKKSDIGDIYGVKGFAFRTKTGEISIHAEEITLLSK
HHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHH
SLQILPEKFHGLTDTDTRYRQRYVDLIMNQESKEVFIKRSQILKEIRNFLAGRDFMEVET
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PMLVSNAGGAAARPFETHYNALNEDVKLRISLELYLKRLIVGGLERVYEIGRVFRNEGVD
CEEEECCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TRHNPEFTLMELYQAYTDYEGMMELTESMFRYLAEKVCGSTKISYNGVEIDLGKPFARMT
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCHHHHH
MIDAIKKYAGVDFDQVPDDAAAKKLADEHHIEYEERHKKGDIVNLFFEEYCEKELIQPTF
HHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCE
IMDHPIEISPLTKKKPSDPTKVERFELFCNTWEMCNAYSELNDPIDQRERFAAQDANAAA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
GDDEAEHTDEDFLNALEIGMPPTGGIGYGIDRLVMLLTDSQAIRDVLLFPTMKSLDK
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
ANQNNNKGGQQQDVNQLLKVRREKLQNLQEAGKDPFQITKYNVTHHSSDVKELYNAHEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
EILGDRKAPDVEGLDDAAKREVINNDYNERREIMDAKPIEVSIAGRMMFKRVMGKASFCN
HHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCC
IQDLKGNIQVYVARDNVGEDSYADFKKSDIGDIYGVKGFAFRTKTGEISIHAEEITLLSK
HHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHH
SLQILPEKFHGLTDTDTRYRQRYVDLIMNQESKEVFIKRSQILKEIRNFLAGRDFMEVET
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PMLVSNAGGAAARPFETHYNALNEDVKLRISLELYLKRLIVGGLERVYEIGRVFRNEGVD
CEEEECCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TRHNPEFTLMELYQAYTDYEGMMELTESMFRYLAEKVCGSTKISYNGVEIDLGKPFARMT
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCHHHHH
MIDAIKKYAGVDFDQVPDDAAAKKLADEHHIEYEERHKKGDIVNLFFEEYCEKELIQPTF
HHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCE
IMDHPIEISPLTKKKPSDPTKVERFELFCNTWEMCNAYSELNDPIDQRERFAAQDANAAA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
GDDEAEHTDEDFLNALEIGMPPTGGIGYGIDRLVMLLTDSQAIRDVLLFPTMKSLDK
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA