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The map label for this gene is greA [H]

Identifier: 238922488

GI number: 238922488

Start: 66345

End: 66902

Strand: Direct

Name: greA [H]

Synonym: EUBREC_0062

Alternate gene names: 238922488

Gene position: 66345-66902 (Clockwise)

Preceding gene: 238922487

Following gene: 238922489

Centisome position: 1.92

GC content: 41.22

Gene sequence:

>558_bases
GTGGGAAGTTTCATTTTAGCAAATCAACATAATAAAATAAAAGAAAAGGAAGATAGAAGGGTAAGAATCATGGATAACAA
GAAAAATATTCTGACATATGCAGGTCTTAAGAAGCTCGAGGATGAGCTTGAGCAGTTAAAGGTTGTTAAGCGTAAAGAGG
TTTCCCAGAAGATCAAGGAGGCTCGTGAGCAGGGTGATTTATCTGAGAATGCTGAGTATGATGCAGCTAAGGATGAGCAG
CGCGATATCGAGGCTCGTATTGAGGAAATCGAGAAAATCCTTAAGAATGCAGAGGTTGTTGTTGAGGAGGAGGTTGACCT
TGACAAGATTTCTATCGGATGTAAGATCCGCATCTTAGATTGTGAGTTTGATGAGGAGCTTGAGTACAAAATTGTTGGTT
CAACAGAGGCAAACAGCTTAAAGGGCAAGATTTCAAACGAGTCACCTGTAGGTAAAGCTCTTCTTGGAAAGAAAGTCGGA
GATACTGTAACTGTTGAGACTCAGGTAGGAGAGCTCACATACAAGGTACTCGAGATCCAGAGAGCAGAAGAGAACTAA

Upstream 100 bases:

>100_bases
TGCTGCGGGGTTTCCGTTGCCGAGGGTTATGGAAACCCGTGGCAGTTCATATTTTTCTTCGGAATATACTGTATACTTTC
CGGTAAATTAAGTCTTTGAA

Downstream 100 bases:

>100_bases
AAGAGGTAAAAAATGGCAAATCAGAATAATAACAAGGGTGGACAGCAGCAGGACGTAAACCAGCTCCTTAAGGTCCGCCG
TGAAAAATTACAGAATTTAC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MGSFILANQHNKIKEKEDRRVRIMDNKKNILTYAGLKKLEDELEQLKVVKRKEVSQKIKEAREQGDLSENAEYDAAKDEQ
RDIEARIEEIEKILKNAEVVVEEEVDLDKISIGCKIRILDCEFDEELEYKIVGSTEANSLKGKISNESPVGKALLGKKVG
DTVTVETQVGELTYKVLEIQRAEEN

Sequences:

>Translated_185_residues
MGSFILANQHNKIKEKEDRRVRIMDNKKNILTYAGLKKLEDELEQLKVVKRKEVSQKIKEAREQGDLSENAEYDAAKDEQ
RDIEARIEEIEKILKNAEVVVEEEVDLDKISIGCKIRILDCEFDEELEYKIVGSTEANSLKGKISNESPVGKALLGKKVG
DTVTVETQVGELTYKVLEIQRAEEN
>Mature_184_residues
GSFILANQHNKIKEKEDRRVRIMDNKKNILTYAGLKKLEDELEQLKVVKRKEVSQKIKEAREQGDLSENAEYDAAKDEQR
DIEARIEEIEKILKNAEVVVEEEVDLDKISIGCKIRILDCEFDEELEYKIVGSTEANSLKGKISNESPVGKALLGKKVGD
TVTVETQVGELTYKVLEIQRAEEN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=151, Percent_Identity=40.3973509933775, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=35.2564102564103, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 21092; Mature: 20961

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFILANQHNKIKEKEDRRVRIMDNKKNILTYAGLKKLEDELEQLKVVKRKEVSQKIKE
CCCEEEECCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AREQGDLSENAEYDAAKDEQRDIEARIEEIEKILKNAEVVVEEEVDLDKISIGCKIRILD
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECEEEEEEE
CEFDEELEYKIVGSTEANSLKGKISNESPVGKALLGKKVGDTVTVETQVGELTYKVLEIQ
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHH
RAEEN
HCCCC
>Mature Secondary Structure 
GSFILANQHNKIKEKEDRRVRIMDNKKNILTYAGLKKLEDELEQLKVVKRKEVSQKIKE
CCEEEECCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AREQGDLSENAEYDAAKDEQRDIEARIEEIEKILKNAEVVVEEEVDLDKISIGCKIRILD
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECEEEEEEE
CEFDEELEYKIVGSTEANSLKGKISNESPVGKALLGKKVGDTVTVETQVGELTYKVLEIQ
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHH
RAEEN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA