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The map label for this gene is cls [C]

Identifier: 238922424

GI number: 238922424

Start: 619376

End: 620935

Strand: Reverse

Name: cls [C]

Synonym: EUBELI_20660

Alternate gene names: 238922424

Gene position: 620935-619376 (Counterclockwise)

Preceding gene: 238922425

Following gene: 238922423

Centisome position: 99.07

GC content: 36.6

Gene sequence:

>1560_bases
ATGAAACAAGATACTTTAGAGGGCAAAGCAAAAACAAAAAATGGGGTAAAACGGCTGTGTTTTTCCATAATCTGCATTCT
TCTGGAAGTGATTTTTATTATTACTATCGTAACACGCTTGAATGAATATGCAGAAATCATAAATTTATTTACAAGGATTT
TGAGTGGGATCTTGGTTTTGGGATTGTACGCATCAAATAAGACATCCTCTATGAAAATGCCTTGGGTCATCTTAATTCTA
ATTTTCCCAATTATGGGTGTAGGCCTGTATTTGCTGATTGGTTTGAATGGTGGCACACATAAAATGCGTGAGCGATATGC
AGAAATTGATAGCAAATTGTTACCAATGCTTCCGGACAGTCAGGAGTGTTTGAGCAGAATAAAAGAAACAATTCCGAAAG
CAGGAAATATAGCAAGTTACATACAAAGAAATTCGCAGTATCCAATCTATCAGAATACGGATATTGTGTATTTTGATGAA
GCAGTGAAAGGATTAGAGGCACAGCTTAAGGATTTGGAGAAAGCACAGAAGTTTATCTTTATGGAATATCATGCGATAGA
AGACGCTGAAGCATGGCATAAGATTCAAGATGTTCTGGAAGAGCGAGTAAAAGCAGGTGTGGAAGTTAGAGTACTCTACG
ATGATATGGGTTCTATAGGCTTTATTAACACAGATTTTGTGAAAAAGATGGAGGCAATAGGAATTCATTGCCGTGTATTC
AATCCGTTTATGCCAGGTTTAAATCTGTTTTTGAACAATCGTGATCATAGAAAAATAACAGTTATTGATGGAAAAGTCGG
ATTTACAGGTGGATATAATCTAGCAAACGAATATTTTAATTACACACACCCGTATGGACAGTGGAAAGATACAGGTATTC
GTTTAGAAGGAGATGCTGTTCAGTCGCTTACGGTGACATTTTTGGAAATGTGGAATGCAGTAAGTGCTAAGGATGCTAAT
GATCCTGATTTTAGTAAATACCTTTTCCACTATGATTATGCGGCACAGCAAACTGGGTTTGTTCAACCTTATGCAGACAG
CCCTATGGATAATGAACAAGTAGGAGAAGAAGTTTATATCAGTATGATAAATAAAGCTGAAAAATATTGTTGGTTTATGA
CTCCATATCTAATCATAACAGATGAAATGACGCATGCGTTATGTCTGGCTGCTAAGCGAGGGGTAGACGTAAGAATTATC
ACACCTGGTATCCCTGATAAAAAATTCATTTATAATATAACTCGTTCTTTTTATCATGGATTAGTTAAACATGGTGTTCG
TGTTTATGAGTGGACTCCTGGTTTCTGTCATGCGAAAATGAGTGTGGCAGATGACTGTATGGCAACTTGTGGAACAATTA
ATTTGGATTATCGAAGTTTATATCACCATTTTGAAAACGGCTGTTTTATGGCAGATTGTCAGGCAGTTGTGGATATAAAA
AATGATCTGATAAGAACGATGGGAGAATGTCGTGATGTGACAGACCAATATCAAACCGGACGAAGTGCATATTTGCGACT
GGGACAATTATTTATGAGATTATTTGCTGGATTGCTATAA

Upstream 100 bases:

>100_bases
TCATTGAAGCAGATAATGAAATGCAGAGAATAAAAGACAAAATTGGTATGTTCTACGAAATTTAGGGAGAAAACTGAGCA
AGTAGGAAGGTGGAATATAT

Downstream 100 bases:

>100_bases
GAATCTGATGAAACGACCTTTCAAAAAACAGTTGATTTAGAAGTTAACTGTTTTTGAAAGGCCGTTTTTGCTATATCTAA
CAGTCTGTTGTACAAAGAAG

Product: cardiolipin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKQDTLEGKAKTKNGVKRLCFSIICILLEVIFIITIVTRLNEYAEIINLFTRILSGILVLGLYASNKTSSMKMPWVILIL
IFPIMGVGLYLLIGLNGGTHKMRERYAEIDSKLLPMLPDSQECLSRIKETIPKAGNIASYIQRNSQYPIYQNTDIVYFDE
AVKGLEAQLKDLEKAQKFIFMEYHAIEDAEAWHKIQDVLEERVKAGVEVRVLYDDMGSIGFINTDFVKKMEAIGIHCRVF
NPFMPGLNLFLNNRDHRKITVIDGKVGFTGGYNLANEYFNYTHPYGQWKDTGIRLEGDAVQSLTVTFLEMWNAVSAKDAN
DPDFSKYLFHYDYAAQQTGFVQPYADSPMDNEQVGEEVYISMINKAEKYCWFMTPYLIITDEMTHALCLAAKRGVDVRII
TPGIPDKKFIYNITRSFYHGLVKHGVRVYEWTPGFCHAKMSVADDCMATCGTINLDYRSLYHHFENGCFMADCQAVVDIK
NDLIRTMGECRDVTDQYQTGRSAYLRLGQLFMRLFAGLL

Sequences:

>Translated_519_residues
MKQDTLEGKAKTKNGVKRLCFSIICILLEVIFIITIVTRLNEYAEIINLFTRILSGILVLGLYASNKTSSMKMPWVILIL
IFPIMGVGLYLLIGLNGGTHKMRERYAEIDSKLLPMLPDSQECLSRIKETIPKAGNIASYIQRNSQYPIYQNTDIVYFDE
AVKGLEAQLKDLEKAQKFIFMEYHAIEDAEAWHKIQDVLEERVKAGVEVRVLYDDMGSIGFINTDFVKKMEAIGIHCRVF
NPFMPGLNLFLNNRDHRKITVIDGKVGFTGGYNLANEYFNYTHPYGQWKDTGIRLEGDAVQSLTVTFLEMWNAVSAKDAN
DPDFSKYLFHYDYAAQQTGFVQPYADSPMDNEQVGEEVYISMINKAEKYCWFMTPYLIITDEMTHALCLAAKRGVDVRII
TPGIPDKKFIYNITRSFYHGLVKHGVRVYEWTPGFCHAKMSVADDCMATCGTINLDYRSLYHHFENGCFMADCQAVVDIK
NDLIRTMGECRDVTDQYQTGRSAYLRLGQLFMRLFAGLL
>Mature_519_residues
MKQDTLEGKAKTKNGVKRLCFSIICILLEVIFIITIVTRLNEYAEIINLFTRILSGILVLGLYASNKTSSMKMPWVILIL
IFPIMGVGLYLLIGLNGGTHKMRERYAEIDSKLLPMLPDSQECLSRIKETIPKAGNIASYIQRNSQYPIYQNTDIVYFDE
AVKGLEAQLKDLEKAQKFIFMEYHAIEDAEAWHKIQDVLEERVKAGVEVRVLYDDMGSIGFINTDFVKKMEAIGIHCRVF
NPFMPGLNLFLNNRDHRKITVIDGKVGFTGGYNLANEYFNYTHPYGQWKDTGIRLEGDAVQSLTVTFLEMWNAVSAKDAN
DPDFSKYLFHYDYAAQQTGFVQPYADSPMDNEQVGEEVYISMINKAEKYCWFMTPYLIITDEMTHALCLAAKRGVDVRII
TPGIPDKKFIYNITRSFYHGLVKHGVRVYEWTPGFCHAKMSVADDCMATCGTINLDYRSLYHHFENGCFMADCQAVVDIK
NDLIRTMGECRDVTDQYQTGRSAYLRLGQLFMRLFAGLL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=467, Percent_Identity=24.6252676659529, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787007, Length=337, Percent_Identity=28.486646884273, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59337; Mature: 59337

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDTLEGKAKTKNGVKRLCFSIICILLEVIFIITIVTRLNEYAEIINLFTRILSGILVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYASNKTSSMKMPWVILILIFPIMGVGLYLLIGLNGGTHKMRERYAEIDSKLLPMLPDS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCH
QECLSRIKETIPKAGNIASYIQRNSQYPIYQNTDIVYFDEAVKGLEAQLKDLEKAQKFIF
HHHHHHHHHHCCCCCCHHHHHHCCCCCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MEYHAIEDAEAWHKIQDVLEERVKAGVEVRVLYDDMGSIGFINTDFVKKMEAIGIHCRVF
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCEEEEEE
NPFMPGLNLFLNNRDHRKITVIDGKVGFTGGYNLANEYFNYTHPYGQWKDTGIRLEGDAV
CCCCCCCCEEECCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCHH
QSLTVTFLEMWNAVSAKDANDPDFSKYLFHYDYAAQQTGFVQPYADSPMDNEQVGEEVYI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
SMINKAEKYCWFMTPYLIITDEMTHALCLAAKRGVDVRIITPGIPDKKFIYNITRSFYHG
HHHHHHHHHEEEECCEEEEECHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
LVKHGVRVYEWTPGFCHAKMSVADDCMATCGTINLDYRSLYHHFENGCFMADCQAVVDIK
HHHCCCEEEEECCCHHEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEHHHHHHHHHH
NDLIRTMGECRDVTDQYQTGRSAYLRLGQLFMRLFAGLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQDTLEGKAKTKNGVKRLCFSIICILLEVIFIITIVTRLNEYAEIINLFTRILSGILVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYASNKTSSMKMPWVILILIFPIMGVGLYLLIGLNGGTHKMRERYAEIDSKLLPMLPDS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCH
QECLSRIKETIPKAGNIASYIQRNSQYPIYQNTDIVYFDEAVKGLEAQLKDLEKAQKFIF
HHHHHHHHHHCCCCCCHHHHHHCCCCCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MEYHAIEDAEAWHKIQDVLEERVKAGVEVRVLYDDMGSIGFINTDFVKKMEAIGIHCRVF
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCEEEEEE
NPFMPGLNLFLNNRDHRKITVIDGKVGFTGGYNLANEYFNYTHPYGQWKDTGIRLEGDAV
CCCCCCCCEEECCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCHH
QSLTVTFLEMWNAVSAKDANDPDFSKYLFHYDYAAQQTGFVQPYADSPMDNEQVGEEVYI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
SMINKAEKYCWFMTPYLIITDEMTHALCLAAKRGVDVRIITPGIPDKKFIYNITRSFYHG
HHHHHHHHHEEEECCEEEEECHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
LVKHGVRVYEWTPGFCHAKMSVADDCMATCGTINLDYRSLYHHFENGCFMADCQAVVDIK
HHHCCCEEEEECCCHHEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEHHHHHHHHHH
NDLIRTMGECRDVTDQYQTGRSAYLRLGQLFMRLFAGLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]