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The map label for this gene is hisJ [H]

Identifier: 238922203

GI number: 238922203

Start: 403226

End: 404017

Strand: Reverse

Name: hisJ [H]

Synonym: EUBELI_20438

Alternate gene names: 238922203

Gene position: 404017-403226 (Counterclockwise)

Preceding gene: 238922207

Following gene: 238922202

Centisome position: 64.46

GC content: 39.77

Gene sequence:

>792_bases
ATGCTAAGCATATATGCAGCTTCTTTTTATGGCTGTGGGAAAAAAGAATGGTCAGACAGCCACAATAATGAGGCTGGATT
GCCGGAAATAGTCATAGGAAGTGATAACTATCCGCCATATAATTATGTTGATACAGATGGCAATGCTACTGGAATTGATG
TTGAGCTTGCTACGGAAGCATTTAAAAGAATGGGCTATAAGGCCAGATTCATATATATTGACTGGGAGGATAAGAAGAAT
CTTCTTGCTGACAGAACAATTGATTGTGCATGGGGAAGCTTTAGCATGGATGGCAGGGAGAATGAATACAGATGGGCAGG
ACCTTATATGACCAGCCGTCAGGTTGTTGCTGTGAATATGGAAAGTGATATATATTCTCTTGAGGATCTGGAAGGAAAGA
CGATTGCAGTACAGACTACAACAAGACCTGAGAAGATATTTCTTAGCAAGACTGATGAGCGTATCCCGGCTATAAGGTCA
TTAATAGCTTTGCAGAAGAGAGAACTTATATATCCTTTTTTAAGCAAAGGCTATGCAGATGCTCTGGCAGCTCATGAAAC
TGCAATACGCCAGTACATGGAAGATTATAATATTAAGTTCAGGATTCTTGACGAACCATTAGAGACAGTAGGACTGGGAG
TAGCATTTGACAAAAATGATGACCGTGGGATAGAGAAGCAGCTTACAGAAATTCTTAACCAGATGAGAAATGATGGTACA
GAGAAGAAGATAATCAGCAGATATATTCCTGATGCTGATAAATATCTGGAGGTGGACAGTTATGGAAAATAA

Upstream 100 bases:

>100_bases
TAATCAGCAGCTTTTTGTATTATAATAGAACATATTTTATAAATATGTAGGAGATGGAAAGTAAGTGCGTAGGATTAAAA
GCTTTTTGATTATAATATGT

Downstream 100 bases:

>100_bases
TACCATTACCAGAAAGAAAAGAAAAAGAATATGGCCGATTGAGATTATAGCAGGCCTGTTTATAATGATGATTGTGTTTT
TTATTGCTGTAAATGTTGAT

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLSIYAASFYGCGKKEWSDSHNNEAGLPEIVIGSDNYPPYNYVDTDGNATGIDVELATEAFKRMGYKARFIYIDWEDKKN
LLADRTIDCAWGSFSMDGRENEYRWAGPYMTSRQVVAVNMESDIYSLEDLEGKTIAVQTTTRPEKIFLSKTDERIPAIRS
LIALQKRELIYPFLSKGYADALAAHETAIRQYMEDYNIKFRILDEPLETVGLGVAFDKNDDRGIEKQLTEILNQMRNDGT
EKKIISRYIPDADKYLEVDSYGK

Sequences:

>Translated_263_residues
MLSIYAASFYGCGKKEWSDSHNNEAGLPEIVIGSDNYPPYNYVDTDGNATGIDVELATEAFKRMGYKARFIYIDWEDKKN
LLADRTIDCAWGSFSMDGRENEYRWAGPYMTSRQVVAVNMESDIYSLEDLEGKTIAVQTTTRPEKIFLSKTDERIPAIRS
LIALQKRELIYPFLSKGYADALAAHETAIRQYMEDYNIKFRILDEPLETVGLGVAFDKNDDRGIEKQLTEILNQMRNDGT
EKKIISRYIPDADKYLEVDSYGK
>Mature_263_residues
MLSIYAASFYGCGKKEWSDSHNNEAGLPEIVIGSDNYPPYNYVDTDGNATGIDVELATEAFKRMGYKARFIYIDWEDKKN
LLADRTIDCAWGSFSMDGRENEYRWAGPYMTSRQVVAVNMESDIYSLEDLEGKTIAVQTTTRPEKIFLSKTDERIPAIRS
LIALQKRELIYPFLSKGYADALAAHETAIRQYMEDYNIKFRILDEPLETVGLGVAFDKNDDRGIEKQLTEILNQMRNDGT
EKKIISRYIPDADKYLEVDSYGK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=247, Percent_Identity=29.9595141700405, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=26.953125, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30078; Mature: 30078

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIYAASFYGCGKKEWSDSHNNEAGLPEIVIGSDNYPPYNYVDTDGNATGIDVELATEA
CEEEEEEHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEECCCCCCEEEEHHHHHH
FKRMGYKARFIYIDWEDKKNLLADRTIDCAWGSFSMDGRENEYRWAGPYMTSRQVVAVNM
HHHCCCEEEEEEEEECCCCCHHHCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEEEE
ESDIYSLEDLEGKTIAVQTTTRPEKIFLSKTDERIPAIRSLIALQKRELIYPFLSKGYAD
CCCCEEHHCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ALAAHETAIRQYMEDYNIKFRILDEPLETVGLGVAFDKNDDRGIEKQLTEILNQMRNDGT
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCH
EKKIISRYIPDADKYLEVDSYGK
HHHHHHHHCCCHHHEEECCCCCC
>Mature Secondary Structure
MLSIYAASFYGCGKKEWSDSHNNEAGLPEIVIGSDNYPPYNYVDTDGNATGIDVELATEA
CEEEEEEHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEECCCCCCEEEEHHHHHH
FKRMGYKARFIYIDWEDKKNLLADRTIDCAWGSFSMDGRENEYRWAGPYMTSRQVVAVNM
HHHCCCEEEEEEEEECCCCCHHHCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEEEE
ESDIYSLEDLEGKTIAVQTTTRPEKIFLSKTDERIPAIRSLIALQKRELIYPFLSKGYAD
CCCCEEHHCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ALAAHETAIRQYMEDYNIKFRILDEPLETVGLGVAFDKNDDRGIEKQLTEILNQMRNDGT
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCH
EKKIISRYIPDADKYLEVDSYGK
HHHHHHHHCCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]