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The map label for this gene is ywbI [H]

Identifier: 238921978

GI number: 238921978

Start: 174112

End: 175014

Strand: Reverse

Name: ywbI [H]

Synonym: EUBELI_20213

Alternate gene names: 238921978

Gene position: 175014-174112 (Counterclockwise)

Preceding gene: 238921983

Following gene: 238921977

Centisome position: 27.92

GC content: 40.53

Gene sequence:

>903_bases
ATGGATGTTAATTTTGAGTATTACAAGATATTTTATTATGTAGCCAGGTACAAGAATTTTACGAAAGCCGCTCAGGCGTT
AGGAAGCAGCCAGCCTAATGTTACAAGGGCAATGAACTGTCTGGAACAGCAGATTAATTCAACACTGTTTGTAAGAAATA
ACCGGGGAATACAGCTTACACCAGAGGGGGAAAAACTGTATATAAGGGTTACAGCGGCTATGACACAGCTTATGGCAGCA
GAAGAAGAGCTTGCGGACAGTGCAAGTCTTTCACATGGCAGTATTTCCATAGGGGCAAGTGAGACTGCGCTTAATATATT
TCTTCTTGATAAGTTAAAAGCATTTCACATGGTCAATCCCGGAATCCGGCTTAAGATATATAATCATTCAACGCCGGAGG
CGATTGATGCAGTAAAAAATGGACTGGTAGACTTTGCAGTGGTATCATCACCTGTGTACGCAGATTCATCACTAAAGACG
GTGGTGTTGCATTCTTTTCAGGAAATACTTGTCGGAGGAACTACATTTACAGCATTGGGAAGTCAGGAATTATCAATAAG
AGAATTGACGGATTATCCACTGATATGCCTTGGCAGAGAGACTATGACATTCAGATTCTATAAAGATTTATTTATGTCAC
ATGGGGTTGAATTAGAGCCTGATACGGAGGCTGCGACAACAGACCAGATACTTCCTCTTGTAAAATGTGAATTAGGACTT
GCATTCTTACCAGAGACAATGGCGCAGGAATCGATTGATAAGAAAGAGATTGTGAAGATATCTCTTAAGGATAATATTCC
GAAGAGAAATATATGTCTTGTCTATGACAGCAGACACCCGGTGAGTTCGGCGGCAAGAAAGTTGAGAAAAACAATAGGGG
AGATTAATTATGGGGAGAATTAA

Upstream 100 bases:

>100_bases
TTCGGCTAGATTATACTATTGTGGTAGAAATAAGTAAAAACTATAATTGATATAAATGCTATAACAATATGATATGTCAG
ATATTACGGAGCGATAAGTT

Downstream 100 bases:

>100_bases
AGTATGTAACTTTGGCAGAATTATGCTGAAAATATTCTGCTGGACGACAGTAATATTAGCAATATATCTTATATTAGGTA
TTACAGGCTGTTATGAAAAA

Product: LysR family transcriptional regulator, positive regulator for ilvC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDVNFEYYKIFYYVARYKNFTKAAQALGSSQPNVTRAMNCLEQQINSTLFVRNNRGIQLTPEGEKLYIRVTAAMTQLMAA
EEELADSASLSHGSISIGASETALNIFLLDKLKAFHMVNPGIRLKIYNHSTPEAIDAVKNGLVDFAVVSSPVYADSSLKT
VVLHSFQEILVGGTTFTALGSQELSIRELTDYPLICLGRETMTFRFYKDLFMSHGVELEPDTEAATTDQILPLVKCELGL
AFLPETMAQESIDKKEIVKISLKDNIPKRNICLVYDSRHPVSSAARKLRKTIGEINYGEN

Sequences:

>Translated_300_residues
MDVNFEYYKIFYYVARYKNFTKAAQALGSSQPNVTRAMNCLEQQINSTLFVRNNRGIQLTPEGEKLYIRVTAAMTQLMAA
EEELADSASLSHGSISIGASETALNIFLLDKLKAFHMVNPGIRLKIYNHSTPEAIDAVKNGLVDFAVVSSPVYADSSLKT
VVLHSFQEILVGGTTFTALGSQELSIRELTDYPLICLGRETMTFRFYKDLFMSHGVELEPDTEAATTDQILPLVKCELGL
AFLPETMAQESIDKKEIVKISLKDNIPKRNICLVYDSRHPVSSAARKLRKTIGEINYGEN
>Mature_300_residues
MDVNFEYYKIFYYVARYKNFTKAAQALGSSQPNVTRAMNCLEQQINSTLFVRNNRGIQLTPEGEKLYIRVTAAMTQLMAA
EEELADSASLSHGSISIGASETALNIFLLDKLKAFHMVNPGIRLKIYNHSTPEAIDAVKNGLVDFAVVSSPVYADSSLKT
VVLHSFQEILVGGTTFTALGSQELSIRELTDYPLICLGRETMTFRFYKDLFMSHGVELEPDTEAATTDQILPLVKCELGL
AFLPETMAQESIDKKEIVKISLKDNIPKRNICLVYDSRHPVSSAARKLRKTIGEINYGEN

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=296, Percent_Identity=24.6621621621622, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=283, Percent_Identity=22.2614840989399, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI157672245, Length=247, Percent_Identity=25.5060728744939, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=22.6190476190476, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=142, Percent_Identity=28.8732394366197, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081904, Length=145, Percent_Identity=26.2068965517241, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=287, Percent_Identity=20.9059233449477, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790208, Length=164, Percent_Identity=24.390243902439, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789506, Length=291, Percent_Identity=22.3367697594502, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33449; Mature: 33449

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVNFEYYKIFYYVARYKNFTKAAQALGSSQPNVTRAMNCLEQQINSTLFVRNNRGIQLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEC
PEGEKLYIRVTAAMTQLMAAEEELADSASLSHGSISIGASETALNIFLLDKLKAFHMVNP
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEHHHHHHHHCCCC
GIRLKIYNHSTPEAIDAVKNGLVDFAVVSSPVYADSSLKTVVLHSFQEILVGGTTFTALG
CEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECC
SQELSIRELTDYPLICLGRETMTFRFYKDLFMSHGVELEPDTEAATTDQILPLVKCELGL
CCCCCCEECCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCH
AFLPETMAQESIDKKEIVKISLKDNIPKRNICLVYDSRHPVSSAARKLRKTIGEINYGEN
HHCCHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDVNFEYYKIFYYVARYKNFTKAAQALGSSQPNVTRAMNCLEQQINSTLFVRNNRGIQLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEC
PEGEKLYIRVTAAMTQLMAAEEELADSASLSHGSISIGASETALNIFLLDKLKAFHMVNP
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEHHHHHHHHCCCC
GIRLKIYNHSTPEAIDAVKNGLVDFAVVSSPVYADSSLKTVVLHSFQEILVGGTTFTALG
CEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECC
SQELSIRELTDYPLICLGRETMTFRFYKDLFMSHGVELEPDTEAATTDQILPLVKCELGL
CCCCCCEECCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCH
AFLPETMAQESIDKKEIVKISLKDNIPKRNICLVYDSRHPVSSAARKLRKTIGEINYGEN
HHCCHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]