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The map label for this gene is cbiT [H]

Identifier: 238921947

GI number: 238921947

Start: 145562

End: 146752

Strand: Reverse

Name: cbiT [H]

Synonym: EUBELI_20181

Alternate gene names: 238921947

Gene position: 146752-145562 (Counterclockwise)

Preceding gene: 238921948

Following gene: 238921946

Centisome position: 23.41

GC content: 38.2

Gene sequence:

>1191_bases
ATGAAAAGGGTAATTATAGCTGGAATGGGACCGGGCAGTAAAAGCTGCATTACAGAGGAAGTAAAAGCTGCAATTGATAA
TGCAGAAATTATAATAGGTTCAAAGCGGTTGATAGAGGAATATCCGGATAAAAAGACATTTTATGCTGTAACAGCAGATA
ATATAGTAAGTATTATCGAGCAGGAAGAGGCACAAGAATATGTTGTGCTTATGTCTGGTGATACCGGATTTTACAGTGGA
ACAAGAAAGCTTGCAGATGCGCTTGAAGGAAAATATGAATACCGTATCCTGCCTGGAATATCTTCTGTAATATATTTTGC
CGCAAGAATAGGAAAGCCTTGGGAAAATGCGGCATTTGTAAGTGTGCATGGAAAAAAACAGAGTTACATACCTGTAGTTT
TACAGAATGAGATGACATATTTTCTGACACAGGGAAATGTATCACAGATATGTAAGCAGCTTCAGAGTGTGGGGCTTTCA
AAGGCACATGTCTGGATAGGGGAAAATCTTTCATATGAAAATGAAAAGATATCCAGTGGTGAAGTGTCAGAATTTACAGA
ATATGAGTCAGCAGGGCTTACTGTAATGGCAGTATACAATGATTATTCACATACATTTACTATAACAGGAATTCCTGACA
GTTATTTTATCCGTTCAGATGTGCCAATGACTAAGAGGGAAATACGGGCAAGTGTAATCAGCCGTATGGCAGTCAGAAAG
AATTCATGTGTGTATGATATAGGCGCAGGAACAGGTTCTGTTTCGGTAGAGCTTGCACTGCATGCACCATACGGAACTGT
ATATGCGGTTGAGAGAACAGAAGAAGGATGCAGTCTTATTGCCCGTAATGCAGAGAAATTCGGACTTAATAACATAGAGA
TTATACATGGCAATGCAGCAGATGTAATTGATAATCTTCCAGTGCCGGATGAAGTGTTTATTGGTGGAAGTGGCGGTGAA
TCAGAATACATTTTTGACAGTATTCTTAGAAAAAATCCCAATGTCCATGTTGTGGCTACAGCAGTAACTTTAGAAACACT
ACAGGATATGATTTCATTAATGGAAAAGAATAATATGAGCGTACTTGATATAGTCCAGATTGCTGTAACACAGATAAAGA
AAACAGGCAGATATCATATGATGTCTGCAAATAATCCAGTATTTATTATAGAGGGAAGGAGAATGCAATGA

Upstream 100 bases:

>100_bases
GCGGCCATTGGAAAATGGTGGTAAGAATCTTTCAGAAATTATAGCGTATCTTGAAGAAAATATTGATTCACAGTGTTAAC
AGGTTAAGATGGAGTGAGTA

Downstream 100 bases:

>100_bases
GTCGTATAATGATAGCCGGAACAGGCAGTGGGTGTGGCAAAACAACAATTGTCTGTGGTTTATGCCAGTGCTTTAAAGAT
ATGGGACTTAAGACATCAGC

Product: precorrin-6Y C5,15-methyltransferase / precorrin-8W decarboxylase

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKRVIIAGMGPGSKSCITEEVKAAIDNAEIIIGSKRLIEEYPDKKTFYAVTADNIVSIIEQEEAQEYVVLMSGDTGFYSG
TRKLADALEGKYEYRILPGISSVIYFAARIGKPWENAAFVSVHGKKQSYIPVVLQNEMTYFLTQGNVSQICKQLQSVGLS
KAHVWIGENLSYENEKISSGEVSEFTEYESAGLTVMAVYNDYSHTFTITGIPDSYFIRSDVPMTKREIRASVISRMAVRK
NSCVYDIGAGTGSVSVELALHAPYGTVYAVERTEEGCSLIARNAEKFGLNNIEIIHGNAADVIDNLPVPDEVFIGGSGGE
SEYIFDSILRKNPNVHVVATAVTLETLQDMISLMEKNNMSVLDIVQIAVTQIKKTGRYHMMSANNPVFIIEGRRMQ

Sequences:

>Translated_396_residues
MKRVIIAGMGPGSKSCITEEVKAAIDNAEIIIGSKRLIEEYPDKKTFYAVTADNIVSIIEQEEAQEYVVLMSGDTGFYSG
TRKLADALEGKYEYRILPGISSVIYFAARIGKPWENAAFVSVHGKKQSYIPVVLQNEMTYFLTQGNVSQICKQLQSVGLS
KAHVWIGENLSYENEKISSGEVSEFTEYESAGLTVMAVYNDYSHTFTITGIPDSYFIRSDVPMTKREIRASVISRMAVRK
NSCVYDIGAGTGSVSVELALHAPYGTVYAVERTEEGCSLIARNAEKFGLNNIEIIHGNAADVIDNLPVPDEVFIGGSGGE
SEYIFDSILRKNPNVHVVATAVTLETLQDMISLMEKNNMSVLDIVQIAVTQIKKTGRYHMMSANNPVFIIEGRRMQ
>Mature_396_residues
MKRVIIAGMGPGSKSCITEEVKAAIDNAEIIIGSKRLIEEYPDKKTFYAVTADNIVSIIEQEEAQEYVVLMSGDTGFYSG
TRKLADALEGKYEYRILPGISSVIYFAARIGKPWENAAFVSVHGKKQSYIPVVLQNEMTYFLTQGNVSQICKQLQSVGLS
KAHVWIGENLSYENEKISSGEVSEFTEYESAGLTVMAVYNDYSHTFTITGIPDSYFIRSDVPMTKREIRASVISRMAVRK
NSCVYDIGAGTGSVSVELALHAPYGTVYAVERTEEGCSLIARNAEKFGLNNIEIIHGNAADVIDNLPVPDEVFIGGSGGE
SEYIFDSILRKNPNVHVVATAVTLETLQDMISLMEKNNMSVLDIVQIAVTQIKKTGRYHMMSANNPVFIIEGRRMQ

Specific function: Probably catalyzes the methylation of either C-15 or C-5 in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of C-15 would probably be followed by a spontaneous decarboxylation of C-12 [H]

COG id: COG2242

COG function: function code H; Precorrin-6B methylase 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Archaeal-type CbiT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014008
- InterPro:   IPR000241 [H]

Pfam domain/function: PF01170 UPF0020 [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 43697; Mature: 43697

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVIIAGMGPGSKSCITEEVKAAIDNAEIIIGSKRLIEEYPDKKTFYAVTADNIVSIIE
CCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCEEEEEEHHHHHHHHH
QEEAQEYVVLMSGDTGFYSGTRKLADALEGKYEYRILPGISSVIYFAARIGKPWENAAFV
HHCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEE
SVHGKKQSYIPVVLQNEMTYFLTQGNVSQICKQLQSVGLSKAHVWIGENLSYENEKISSG
EEECCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
EVSEFTEYESAGLTVMAVYNDYSHTFTITGIPDSYFIRSDVPMTKREIRASVISRMAVRK
CCHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHC
NSCVYDIGAGTGSVSVELALHAPYGTVYAVERTEEGCSLIARNAEKFGLNNIEIIHGNAA
CCEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCCHHHHHCCHHHCCCCCEEEEECCHH
DVIDNLPVPDEVFIGGSGGESEYIFDSILRKNPNVHVVATAVTLETLQDMISLMEKNNMS
HHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCC
VLDIVQIAVTQIKKTGRYHMMSANNPVFIIEGRRMQ
HHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCC
>Mature Secondary Structure
MKRVIIAGMGPGSKSCITEEVKAAIDNAEIIIGSKRLIEEYPDKKTFYAVTADNIVSIIE
CCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCEEEEEEHHHHHHHHH
QEEAQEYVVLMSGDTGFYSGTRKLADALEGKYEYRILPGISSVIYFAARIGKPWENAAFV
HHCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEE
SVHGKKQSYIPVVLQNEMTYFLTQGNVSQICKQLQSVGLSKAHVWIGENLSYENEKISSG
EEECCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
EVSEFTEYESAGLTVMAVYNDYSHTFTITGIPDSYFIRSDVPMTKREIRASVISRMAVRK
CCHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHC
NSCVYDIGAGTGSVSVELALHAPYGTVYAVERTEEGCSLIARNAEKFGLNNIEIIHGNAA
CCEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCCHHHHHCCHHHCCCCCEEEEECCHH
DVIDNLPVPDEVFIGGSGGESEYIFDSILRKNPNVHVVATAVTLETLQDMISLMEKNNMS
HHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCC
VLDIVQIAVTQIKKTGRYHMMSANNPVFIIEGRRMQ
HHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA