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The map label for this gene is cobB [H]

Identifier: 238921946

GI number: 238921946

Start: 143124

End: 145565

Strand: Reverse

Name: cobB [H]

Synonym: EUBELI_20180

Alternate gene names: 238921946

Gene position: 145565-143124 (Counterclockwise)

Preceding gene: 238921947

Following gene: 238921945

Centisome position: 23.23

GC content: 40.42

Gene sequence:

>2442_bases
ATGAGTCGTATAATGATAGCCGGAACAGGCAGTGGGTGTGGCAAAACAACAATTGTCTGTGGTTTATGCCAGTGCTTTAA
AGATATGGGACTTAAGACATCAGCATTAAAATGCGGACCTGATTATATTGATTCTATGTTTCATTCTCGTGTACTGAATA
TGCGTACAGGCAATCTTGACAGCTGGTTCTGTGATGATGAGACAATAAAATATCTGCTTGCACGCAAAGAATGTGAGAGT
GATATAACCATAGTTGAGGGCGTTATGGGATACTTTGACGGCCAGGGCTTTGGCACGAAGGGAAGCTCCTATGATATAGC
AGGCATTACGGATACACCGGTTATACTTATAGTAAACTGCCGTGGGATGAGTAATTCAATAGGTGCAGTCATAAAAGGAT
ATACAGGATATGAAAAAGATAGTCATATCAGAGGTGTGATATTTAACAATTTATCATCCAGATTATATAAAGATGCTGCA
CAGATGGTTAAAGAGATGGGGATAGAACCATTAGGCTATCTGCCATATAAGAAGGAGGCCGTTCTTGAAAGCCGTCATCT
TGGACTTGTTACATCCGCAGAGGTTGAACATTTTCAGGAAAAAGTCACCTGTATTGCAAGTCAGATGAAAGAAACGTTGG
ATATAGACGGGATTCTTAAGATTGCAGAAAACGCCTCTGAGAATGAAATCCATTACAAAGAAAAGAAAAAGAGAAATCTA
AGAATAGCAGTTGCAAAGGATGAAGCATTCTGTTTTCTTTATGAAGATAACCTTGATTATTTAAGACAGAGTGGATGTGA
ACTTGCATATTTCAGTCCTCTTAAAGACAGCAGTCTTCCTGATAATATAGACGGATTATTATTATATGGGGGATATCCTG
AGCTTCATGCAGAAGAACTGTCAGAAAATATATCAATGCGAAAGGATATTGCTGACAGAATACGGTCAGGTCTTCCATGT
ATAGCGGAGTGTGGCGGGTTTCTATATCTGCATGAGTATCTTGAAACTCCGGAAAAAGAAAAATATCCAATGGCTGGAAT
TATCAGAGGAACAGGTTATAATGCCGGAAAATTACAGCGTTTCGGGTATATGAGCGTTAAGGCTGCAAAAGATACAATGC
TTGCAGATAAAAATCAAAGCTTCCGTGCACATGAATTTCATTATTGGAACAGTGACTGTCCGGGTAGTGATTATGAAGTC
ATAAAGGCTTCTGATAACAGCACAGCTTCTGCCGGTTACGGAAGTGATACTTTATATGCAGGATTTCCACACATTTATTT
TTATGGTAATGAAAATGTGGCAGAGAGATTTATGGATGCATGTATGAAGTATAAGGAGAACAGCAGACAGGCAGCAGAAC
TTATTCCGGAGCTGGATAATATAAAAGGTATTAATAGAGATGCTGTGATTAAAGCAAAAGCGCACTGGAATGGAATAGCA
AAGCCATTACATGGTCTTGGGCTTATGGAAGAAATAATAACACAGATAGCAGGAATCCAGAATACAGTTGATGTACATAT
AGACAAAAGGGCTGTTATTGTAATGTGTGCAGACAACGGAATAGTTGAAGAGGGAGTTACACAGACAGGACAGGATGTGA
CTGCAGTTGTAAGCTGCAATATGGCAGATGGAATATCGAGTGTATGCAGAATGGCAGCTTGCAGTAAAACAGAAGTGATT
CCTGTAAATATAGGTATTGCAGCAGATAAACTTGCGGATGGAACAGATGTAGGAACTTATAAAGGTCTTGTAAACAGACG
GGTTATGACAGGTACGCGAAATTTCCTTAAAGAACCTGCAATGTCACAGGAACAGCTCATACAGGCAGTCCACGAAGGAA
TTAAGCAGGTGGAATGGTGCAGTGAACAAGGATATAATATTCTTGCTACAGGAGAGATGGGGATTGGCAATACAACTACG
AGTACAGCACTTGCAAGTATATTATTAAACCTTGAGCCAGAAGCTGTTACCGGAAGAGGTGCAGGACTTGATGATTCAGG
GCTTAAAAGGAAAGTTGAAGTCATTACAAAGGCTAAAGAAATGTATGGCAGATACGCAGACAATCCATTAAAACTTTTAC
AGAGTATAGGCGGGCTTGATATAGCAGGACTTGTGGGCGTGTATATCGGCGGAGCAGTGTATGGGATACCGGTTGTTGCT
GATGGTGTTATCGCGGCGGTTGCAGCGCTTATTGCCGTGAAGCTGCAGCCGGAGATTAATGATTATATTATTGTATCTCA
TCAGGGAAAAGAACCGGCAATGAAAGCACTTCTTGACAGTCTGGGAAAGAAAGCTGTTATTCATGCAGAGCTTGCATTAG
GAGAAGGAACAGGGGCAGTTATGATGTTTCCGCTTTTTGACATGGCTTTGCAGGTGTACAGGGAAAATACTACATTTGAC
GATATACAGATAGCTGCATATGAGGATTATGGAAAATGTTGA

Upstream 100 bases:

>100_bases
ATATAGTCCAGATTGCTGTAACACAGATAAAGAAAACAGGCAGATATCATATGATGTCTGCAAATAATCCAGTATTTATT
ATAGAGGGAAGGAGAATGCA

Downstream 100 bases:

>100_bases
TTATAATTACAGGGGTAAGCGGCAGCGGCAAGTCAGAATATGCTGAACAGATATGCTGCCGGCTTGCCGGAGATAATAAA
AAATATTATGTGGCAACCAT

Product: hydrogenobyrinic acid a,c-diamide synthase

Products: Nicotinate; N1-(5-Phospho-Alpha-D- Ribosyl)-5,6-Dimethylbenzimidazole. [C]

Alternate protein names: NA

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MSRIMIAGTGSGCGKTTIVCGLCQCFKDMGLKTSALKCGPDYIDSMFHSRVLNMRTGNLDSWFCDDETIKYLLARKECES
DITIVEGVMGYFDGQGFGTKGSSYDIAGITDTPVILIVNCRGMSNSIGAVIKGYTGYEKDSHIRGVIFNNLSSRLYKDAA
QMVKEMGIEPLGYLPYKKEAVLESRHLGLVTSAEVEHFQEKVTCIASQMKETLDIDGILKIAENASENEIHYKEKKKRNL
RIAVAKDEAFCFLYEDNLDYLRQSGCELAYFSPLKDSSLPDNIDGLLLYGGYPELHAEELSENISMRKDIADRIRSGLPC
IAECGGFLYLHEYLETPEKEKYPMAGIIRGTGYNAGKLQRFGYMSVKAAKDTMLADKNQSFRAHEFHYWNSDCPGSDYEV
IKASDNSTASAGYGSDTLYAGFPHIYFYGNENVAERFMDACMKYKENSRQAAELIPELDNIKGINRDAVIKAKAHWNGIA
KPLHGLGLMEEIITQIAGIQNTVDVHIDKRAVIVMCADNGIVEEGVTQTGQDVTAVVSCNMADGISSVCRMAACSKTEVI
PVNIGIAADKLADGTDVGTYKGLVNRRVMTGTRNFLKEPAMSQEQLIQAVHEGIKQVEWCSEQGYNILATGEMGIGNTTT
STALASILLNLEPEAVTGRGAGLDDSGLKRKVEVITKAKEMYGRYADNPLKLLQSIGGLDIAGLVGVYIGGAVYGIPVVA
DGVIAAVAALIAVKLQPEINDYIIVSHQGKEPAMKALLDSLGKKAVIHAELALGEGTGAVMMFPLFDMALQVYRENTTFD
DIQIAAYEDYGKC

Sequences:

>Translated_813_residues
MSRIMIAGTGSGCGKTTIVCGLCQCFKDMGLKTSALKCGPDYIDSMFHSRVLNMRTGNLDSWFCDDETIKYLLARKECES
DITIVEGVMGYFDGQGFGTKGSSYDIAGITDTPVILIVNCRGMSNSIGAVIKGYTGYEKDSHIRGVIFNNLSSRLYKDAA
QMVKEMGIEPLGYLPYKKEAVLESRHLGLVTSAEVEHFQEKVTCIASQMKETLDIDGILKIAENASENEIHYKEKKKRNL
RIAVAKDEAFCFLYEDNLDYLRQSGCELAYFSPLKDSSLPDNIDGLLLYGGYPELHAEELSENISMRKDIADRIRSGLPC
IAECGGFLYLHEYLETPEKEKYPMAGIIRGTGYNAGKLQRFGYMSVKAAKDTMLADKNQSFRAHEFHYWNSDCPGSDYEV
IKASDNSTASAGYGSDTLYAGFPHIYFYGNENVAERFMDACMKYKENSRQAAELIPELDNIKGINRDAVIKAKAHWNGIA
KPLHGLGLMEEIITQIAGIQNTVDVHIDKRAVIVMCADNGIVEEGVTQTGQDVTAVVSCNMADGISSVCRMAACSKTEVI
PVNIGIAADKLADGTDVGTYKGLVNRRVMTGTRNFLKEPAMSQEQLIQAVHEGIKQVEWCSEQGYNILATGEMGIGNTTT
STALASILLNLEPEAVTGRGAGLDDSGLKRKVEVITKAKEMYGRYADNPLKLLQSIGGLDIAGLVGVYIGGAVYGIPVVA
DGVIAAVAALIAVKLQPEINDYIIVSHQGKEPAMKALLDSLGKKAVIHAELALGEGTGAVMMFPLFDMALQVYRENTTFD
DIQIAAYEDYGKC
>Mature_812_residues
SRIMIAGTGSGCGKTTIVCGLCQCFKDMGLKTSALKCGPDYIDSMFHSRVLNMRTGNLDSWFCDDETIKYLLARKECESD
ITIVEGVMGYFDGQGFGTKGSSYDIAGITDTPVILIVNCRGMSNSIGAVIKGYTGYEKDSHIRGVIFNNLSSRLYKDAAQ
MVKEMGIEPLGYLPYKKEAVLESRHLGLVTSAEVEHFQEKVTCIASQMKETLDIDGILKIAENASENEIHYKEKKKRNLR
IAVAKDEAFCFLYEDNLDYLRQSGCELAYFSPLKDSSLPDNIDGLLLYGGYPELHAEELSENISMRKDIADRIRSGLPCI
AECGGFLYLHEYLETPEKEKYPMAGIIRGTGYNAGKLQRFGYMSVKAAKDTMLADKNQSFRAHEFHYWNSDCPGSDYEVI
KASDNSTASAGYGSDTLYAGFPHIYFYGNENVAERFMDACMKYKENSRQAAELIPELDNIKGINRDAVIKAKAHWNGIAK
PLHGLGLMEEIITQIAGIQNTVDVHIDKRAVIVMCADNGIVEEGVTQTGQDVTAVVSCNMADGISSVCRMAACSKTEVIP
VNIGIAADKLADGTDVGTYKGLVNRRVMTGTRNFLKEPAMSQEQLIQAVHEGIKQVEWCSEQGYNILATGEMGIGNTTTS
TALASILLNLEPEAVTGRGAGLDDSGLKRKVEVITKAKEMYGRYADNPLKLLQSIGGLDIAGLVGVYIGGAVYGIPVVAD
GVIAAVAALIAVKLQPEINDYIIVSHQGKEPAMKALLDSLGKKAVIHAELALGEGTGAVMMFPLFDMALQVYRENTTFDD
IQIAAYEDYGKC

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788300, Length=337, Percent_Identity=33.2344213649852, Blast_Score=195, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 2.4.2.21 [C]

Molecular weight: Translated: 88751; Mature: 88619

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIMIAGTGSGCGKTTIVCGLCQCFKDMGLKTSALKCGPDYIDSMFHSRVLNMRTGNLD
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCC
SWFCDDETIKYLLARKECESDITIVEGVMGYFDGQGFGTKGSSYDIAGITDTPVILIVNC
CEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEC
RGMSNSIGAVIKGYTGYEKDSHIRGVIFNNLSSRLYKDAAQMVKEMGIEPLGYLPYKKEA
CCCCCCCHHHEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VLESRHLGLVTSAEVEHFQEKVTCIASQMKETLDIDGILKIAENASENEIHYKEKKKRNL
HHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHCCCE
RIAVAKDEAFCFLYEDNLDYLRQSGCELAYFSPLKDSSLPDNIDGLLLYGGYPELHAEEL
EEEEECCCEEEEEEECCHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHH
SENISMRKDIADRIRSGLPCIAECGGFLYLHEYLETPEKEKYPMAGIIRGTGYNAGKLQR
HHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHH
FGYMSVKAAKDTMLADKNQSFRAHEFHYWNSDCPGSDYEVIKASDNSTASAGYGSDTLYA
CCCEEEEHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
GFPHIYFYGNENVAERFMDACMKYKENSRQAAELIPELDNIKGINRDAVIKAKAHWNGIA
CCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCCCCCEEEEEEHHCCCHH
KPLHGLGLMEEIITQIAGIQNTVDVHIDKRAVIVMCADNGIVEEGVTQTGQDVTAVVSCN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCHHHCCCCCCCCEEEEEECC
MADGISSVCRMAACSKTEVIPVNIGIAADKLADGTDVGTYKGLVNRRVMTGTRNFLKEPA
CHHHHHHHHHHHHCCCCCEEEEECCEEHHHCCCCCCCHHHHHHHHCHHHHCCHHHHHCCC
MSQEQLIQAVHEGIKQVEWCSEQGYNILATGEMGIGNTTTSTALASILLNLEPEAVTGRG
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
AGLDDSGLKRKVEVITKAKEMYGRYADNPLKLLQSIGGLDIAGLVGVYIGGAVYGIPVVA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
DGVIAAVAALIAVKLQPEINDYIIVSHQGKEPAMKALLDSLGKKAVIHAELALGEGTGAV
HHHHHHHHHHHHEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCE
MMFPLFDMALQVYRENTTFDDIQIAAYEDYGKC
EHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SRIMIAGTGSGCGKTTIVCGLCQCFKDMGLKTSALKCGPDYIDSMFHSRVLNMRTGNLD
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCC
SWFCDDETIKYLLARKECESDITIVEGVMGYFDGQGFGTKGSSYDIAGITDTPVILIVNC
CEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEC
RGMSNSIGAVIKGYTGYEKDSHIRGVIFNNLSSRLYKDAAQMVKEMGIEPLGYLPYKKEA
CCCCCCCHHHEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VLESRHLGLVTSAEVEHFQEKVTCIASQMKETLDIDGILKIAENASENEIHYKEKKKRNL
HHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHCCCE
RIAVAKDEAFCFLYEDNLDYLRQSGCELAYFSPLKDSSLPDNIDGLLLYGGYPELHAEEL
EEEEECCCEEEEEEECCHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHH
SENISMRKDIADRIRSGLPCIAECGGFLYLHEYLETPEKEKYPMAGIIRGTGYNAGKLQR
HHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHH
FGYMSVKAAKDTMLADKNQSFRAHEFHYWNSDCPGSDYEVIKASDNSTASAGYGSDTLYA
CCCEEEEHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
GFPHIYFYGNENVAERFMDACMKYKENSRQAAELIPELDNIKGINRDAVIKAKAHWNGIA
CCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCCCCCEEEEEEHHCCCHH
KPLHGLGLMEEIITQIAGIQNTVDVHIDKRAVIVMCADNGIVEEGVTQTGQDVTAVVSCN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCHHHCCCCCCCCEEEEEECC
MADGISSVCRMAACSKTEVIPVNIGIAADKLADGTDVGTYKGLVNRRVMTGTRNFLKEPA
CHHHHHHHHHHHHCCCCCEEEEECCEEHHHCCCCCCCHHHHHHHHCHHHHCCHHHHHCCC
MSQEQLIQAVHEGIKQVEWCSEQGYNILATGEMGIGNTTTSTALASILLNLEPEAVTGRG
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
AGLDDSGLKRKVEVITKAKEMYGRYADNPLKLLQSIGGLDIAGLVGVYIGGAVYGIPVVA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
DGVIAAVAALIAVKLQPEINDYIIVSHQGKEPAMKALLDSLGKKAVIHAELALGEGTGAV
HHHHHHHHHHHHEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCE
MMFPLFDMALQVYRENTTFDDIQIAAYEDYGKC
EHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Beta-Nicotinate D-Ribonucleotide; Dimethylbenzimidazole [C]

Specific reaction: Beta-Nicotinate D-Ribonucleotide + Dimethylbenzimidazole = Nicotinate + N1-(5-Phospho-Alpha-D- Ribosyl)-5,6-Dimethylbenzimidazole. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]