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The map label for this gene is queE [H]

Identifier: 238921904

GI number: 238921904

Start: 114498

End: 115163

Strand: Reverse

Name: queE [H]

Synonym: EUBELI_20138

Alternate gene names: 238921904

Gene position: 115163-114498 (Counterclockwise)

Preceding gene: 238921905

Following gene: 238921903

Centisome position: 18.37

GC content: 40.39

Gene sequence:

>666_bases
ATGAAAGTTGTTGAGAAATTTATAAGTATCAATGGAGAAGGAAGAAGAACCGGAGAATTGGCGGTGTTTATCAGATTCAA
GGGTTGTAACCTAAATTGCTCCTACTGTGATACAAAGTGGGCCAATGAGCCAGCTTGTGATTATGAGGAGCTGTCACCAG
ATGAGATTTGTGAATATGTCAGTGAGACAGGCATTAAAAATGTGACGCTGACAGGGGGAGAGCCTTTATTACAGAAGGAT
ATCAGGAGTCTGGTAGAAAAATTATTGAACAAATCGGACATTCGAGTAGAGATTGAAACTAATGGAGCGGTTGATATTGC
TCCCCTTGCCAATGCATTTTCAAAAGAAAAACTTAGCCTTACTATGGATTACAAGCTGCCATCCAGCGGCTGTGAGGAGA
AAATGCTTCTCTCCAATATGGAAGTTCTAAGAAAGCAGGACACAGTCAAGTTCGTTTCGGGCAGCATTAATGATTTGGAA
TGTGCAGAGAAAATAATTGAAAAATTTCATCTGACAGAAAGATGCCAGGTATTCATAAGTTCAGTGTTTGGTTCAATTGA
GCCAGTGGAAATCGTGAATTTCATGACAAGTAAAAGGATGAATGATGTGCGTCTTCAGATTCAGATGCACAAGGTAATCT
GGAACCCAGAGGAAAGGGGCGTATAA

Upstream 100 bases:

>100_bases
TGAGCTGATGAGTGCAAAAGGCTTTCCAATCCACAGAGTGGAAGTATACGAGACACCTAATAATGTAGCAGCATACGAAG
AATAAAGGAAAGAAACTAAT

Downstream 100 bases:

>100_bases
TGTGAAAAATCCTTATATCAAAGATTTTTCACGTGCAAATTTGTGCTTTCAGCCCAAAGTTTGCGGGGTGAAGCAAGAAT
GGAGATTATGATGATAGATA

Product: queuosine biosynthesis protein QueE

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKVVEKFISINGEGRRTGELAVFIRFKGCNLNCSYCDTKWANEPACDYEELSPDEICEYVSETGIKNVTLTGGEPLLQKD
IRSLVEKLLNKSDIRVEIETNGAVDIAPLANAFSKEKLSLTMDYKLPSSGCEEKMLLSNMEVLRKQDTVKFVSGSINDLE
CAEKIIEKFHLTERCQVFISSVFGSIEPVEIVNFMTSKRMNDVRLQIQMHKVIWNPEERGV

Sequences:

>Translated_221_residues
MKVVEKFISINGEGRRTGELAVFIRFKGCNLNCSYCDTKWANEPACDYEELSPDEICEYVSETGIKNVTLTGGEPLLQKD
IRSLVEKLLNKSDIRVEIETNGAVDIAPLANAFSKEKLSLTMDYKLPSSGCEEKMLLSNMEVLRKQDTVKFVSGSINDLE
CAEKIIEKFHLTERCQVFISSVFGSIEPVEIVNFMTSKRMNDVRLQIQMHKVIWNPEERGV
>Mature_221_residues
MKVVEKFISINGEGRRTGELAVFIRFKGCNLNCSYCDTKWANEPACDYEELSPDEICEYVSETGIKNVTLTGGEPLLQKD
IRSLVEKLLNKSDIRVEIETNGAVDIAPLANAFSKEKLSLTMDYKLPSSGCEEKMLLSNMEVLRKQDTVKFVSGSINDLE
CAEKIIEKFHLTERCQVFISSVFGSIEPVEIVNFMTSKRMNDVRLQIQMHKVIWNPEERGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=181, Percent_Identity=27.0718232044199, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 25040; Mature: 25040

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVEKFISINGEGRRTGELAVFIRFKGCNLNCSYCDTKWANEPACDYEELSPDEICEYV
CHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SETGIKNVTLTGGEPLLQKDIRSLVEKLLNKSDIRVEIETNGAVDIAPLANAFSKEKLSL
HHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHCCCCEEE
TMDYKLPSSGCEEKMLLSNMEVLRKQDTVKFVSGSINDLECAEKIIEKFHLTERCQVFIS
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVFGSIEPVEIVNFMTSKRMNDVRLQIQMHKVIWNPEERGV
HHHCCCCHHHHHHHHHHCCCCCEEEEEEEEEEEECCHHCCC
>Mature Secondary Structure
MKVVEKFISINGEGRRTGELAVFIRFKGCNLNCSYCDTKWANEPACDYEELSPDEICEYV
CHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SETGIKNVTLTGGEPLLQKDIRSLVEKLLNKSDIRVEIETNGAVDIAPLANAFSKEKLSL
HHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHCCCCEEE
TMDYKLPSSGCEEKMLLSNMEVLRKQDTVKFVSGSINDLECAEKIIEKFHLTERCQVFIS
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVFGSIEPVEIVNFMTSKRMNDVRLQIQMHKVIWNPEERGV
HHHCCCCHHHHHHHHHHCCCCCEEEEEEEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]