Click here to switch to the map view.

The map label for this gene is folE [H]

Identifier: 238921903

GI number: 238921903

Start: 113838

End: 114455

Strand: Reverse

Name: folE [H]

Synonym: EUBELI_20137

Alternate gene names: 238921903

Gene position: 114455-113838 (Counterclockwise)

Preceding gene: 238921904

Following gene: 238921902

Centisome position: 18.26

GC content: 42.88

Gene sequence:

>618_bases
GTGCTTTCAGCCCAAAGTTTGCGGGGTGAAGCAAGAATGGAGATTATGATGATAGATACGAAAGCGATTGAGGAGCATAT
TAGAGGGATTCTTGTTGCATTGGGCGACGACCCGGAGAGAGAGGGCTTAAAGGATACACCAAAGCGAGTTGCGAAGATGT
ACGAGGAGGTCTTTCTTGGGATGAATTACTCCAATCATGAAATTGCGCAGATGTTTGATAAGACATTTGAGGATGAAATT
GAGGAGCTTGATACTTCTAAGGTTGTAGTTATGAAGGACATCGACCTGTTTTCTTACTGCGAGCACCATATGGCACTTAT
GTATGATATGAAGGCGACAGTTGCGTACATTCCTAATGGGAAGGTTATAGGCTTATCAAAGATTGCAAGAATATGCGATA
TGGTTGGAAGAAGACTGCAGTTACAGGAAAGAATCGGTCAGGATATTGCGGACATTATGGCAGAGATAACAGGAAGCGAG
GATGTTGCGGTGGTGCTTGAAGGATATCACAGCTGTGTGTCGGCAAGAGGAATTAAGAAAAACAACACGAGAACAATGAC
TGTAGAGTTAAGAGGAAGCTTTAAGGACGATGCAGCATTACAAATGTATCTAAGATAG

Upstream 100 bases:

>100_bases
GCGTCTTCAGATTCAGATGCACAAGGTAATCTGGAACCCAGAGGAAAGGGGCGTATAATGTGAAAAATCCTTATATCAAA
GATTTTTCACGTGCAAATTT

Downstream 100 bases:

>100_bases
GAGAGATGAGTTATGAAAGCATTAGTATTATCAAGCGGTGGCGTGGATTCCACTACGGCACTGGCATTGGCAGTGAGCCG
ATATGGAAAGGACAATGTAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MLSAQSLRGEARMEIMMIDTKAIEEHIRGILVALGDDPEREGLKDTPKRVAKMYEEVFLGMNYSNHEIAQMFDKTFEDEI
EELDTSKVVVMKDIDLFSYCEHHMALMYDMKATVAYIPNGKVIGLSKIARICDMVGRRLQLQERIGQDIADIMAEITGSE
DVAVVLEGYHSCVSARGIKKNNTRTMTVELRGSFKDDAALQMYLR

Sequences:

>Translated_205_residues
MLSAQSLRGEARMEIMMIDTKAIEEHIRGILVALGDDPEREGLKDTPKRVAKMYEEVFLGMNYSNHEIAQMFDKTFEDEI
EELDTSKVVVMKDIDLFSYCEHHMALMYDMKATVAYIPNGKVIGLSKIARICDMVGRRLQLQERIGQDIADIMAEITGSE
DVAVVLEGYHSCVSARGIKKNNTRTMTVELRGSFKDDAALQMYLR
>Mature_205_residues
MLSAQSLRGEARMEIMMIDTKAIEEHIRGILVALGDDPEREGLKDTPKRVAKMYEEVFLGMNYSNHEIAQMFDKTFEDEI
EELDTSKVVVMKDIDLFSYCEHHMALMYDMKATVAYIPNGKVIGLSKIARICDMVGRRLQLQERIGQDIADIMAEITGSE
DVAVVLEGYHSCVSARGIKKNNTRTMTVELRGSFKDDAALQMYLR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=169, Percent_Identity=37.8698224852071, Blast_Score=124, Evalue=4e-29,
Organism=Homo sapiens, GI4503949, Length=169, Percent_Identity=37.8698224852071, Blast_Score=124, Evalue=4e-29,
Organism=Homo sapiens, GI66932970, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=8e-22,
Organism=Homo sapiens, GI66932972, Length=139, Percent_Identity=38.8489208633094, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788476, Length=199, Percent_Identity=38.6934673366834, Blast_Score=115, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=39.4444444444444, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=38.7640449438202, Blast_Score=130, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656782, Length=171, Percent_Identity=39.1812865497076, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656796, Length=171, Percent_Identity=39.1812865497076, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656791, Length=171, Percent_Identity=39.1812865497076, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23263; Mature: 23263

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
7.3 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAQSLRGEARMEIMMIDTKAIEEHIRGILVALGDDPEREGLKDTPKRVAKMYEEVFLG
CCCCHHCCCCCEEEEEEECHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
MNYSNHEIAQMFDKTFEDEIEELDTSKVVVMKDIDLFSYCEHHMALMYDMKATVAYIPNG
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCC
KVIGLSKIARICDMVGRRLQLQERIGQDIADIMAEITGSEDVAVVLEGYHSCVSARGIKK
CEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
NNTRTMTVELRGSFKDDAALQMYLR
CCCEEEEEEEECCCCCCHHEEEECC
>Mature Secondary Structure
MLSAQSLRGEARMEIMMIDTKAIEEHIRGILVALGDDPEREGLKDTPKRVAKMYEEVFLG
CCCCHHCCCCCEEEEEEECHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
MNYSNHEIAQMFDKTFEDEIEELDTSKVVVMKDIDLFSYCEHHMALMYDMKATVAYIPNG
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCC
KVIGLSKIARICDMVGRRLQLQERIGQDIADIMAEITGSEDVAVVLEGYHSCVSARGIKK
CEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
NNTRTMTVELRGSFKDDAALQMYLR
CCCEEEEEEEECCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]