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The map label for this gene is pflB [H]

Identifier: 238921822

GI number: 238921822

Start: 34382

End: 36640

Strand: Reverse

Name: pflB [H]

Synonym: EUBELI_20055

Alternate gene names: 238921822

Gene position: 36640-34382 (Counterclockwise)

Preceding gene: 238921823

Following gene: 238921820

Centisome position: 5.85

GC content: 40.42

Gene sequence:

>2259_bases
ATGGTTGAGAAGACACAGTGGGCTGGATTCAAGGGAAGACTTTGGAAAGAAGAAATCAATGTAAGAGATTTCATTCAGAA
CAATTACACACCTTATGATGGAGATGAGAGTTTCCTTGCAGGTCCAACAGAAGCAACTAATAAGCTTTGGGGAAAATTAC
AGGAGCTTCAGAAGGAAGAGAGAGCTAAGGGCGGAGTTTTAGACATGGAAACTAAGGTAGTTACAGGTCTTACAGCTTAT
GGCCCAGGATATATAGATGAATCAATGAAGGATCTCGAGCAGGTAGTAGGTCTTCAGACAGATAAGCCACTTAAGAGAGC
ATTTATGCCATATGGTGGTATTAAGATGGCTGAGGAATCTTGCGAGAACTATGGTTATACACCAGATCCTGAATTACACA
AGGTTTTCACAGAATATCATAAGACACATAACCAGGGTGTATTCGATGCCTATACACCAGAGATGAGAGCTGCAAGAAGA
TCTCATATCATCACAGGTCTTCCAGACACATATGGTCGTGGACGTATCGTAGGTGATTACAGAAGAGTTGCTCTTTATGG
TATTGATTATCTTATTAAGTGCAAGGAAGAGGATAAGGCTAACTGTGGATGCGGAGTTATGACTAACGATGTTATCCAGT
TAAGAGAAGAGCTTACAGATCAGATTAATGCTCTTAAGGGAATGAAGGCTATGGCTGCTGCATATGGCTATGATATTTCT
GAGCCAGCTACAACAGCTAAGGAAGCTGTTCAGTGGTTATACTTTGGTTACCTTGCTGCTATCAAGACACAGAACGGTGC
TGCTATGTCAGTAGGTCGTGTTTCTACATTCCTTGATATCTATATCAATAAGGATCTTGAAGCTGGTAAGATTACAGAGG
CTGAGGCACAGGAACTTATCGATCATTTTGTTATGAAGTGCAGAATGGTTAAGTTCGCAAGAATTACATCATACAATGAA
TTATTCTCAGGAGATCCTACATGGGCTACTCTTGAGGTTGGTGGTACAGGTATCGATGGACGTTCTATGGTTACTAAGAA
CGATTACAGATTCCTTCATACTCTTGAGAACATGGGACCTTCACCAGAACCAAACCTTACAGTATTATATTCATCAAGAC
TTCCTGAGAACTTCAAGAAGTATGCTGCTCATATCTCAGTTACAACATCTTCTATCCAGTATGAGAACGATGATGTTATG
AAGGTAACATGGGGAGATGATTATTCAATCTGCTGTTGTGTATCTGCTACACAGACAGGTAAGGAAATGCAGTTCTTCGG
AGCTAGAGCTAACCTTGCTAAGTGCTTACTTTACGCTATCAATGGTGGTGTAGATATGAAGTCTAAGGTTCAGGTTGGAC
CAGCTTATAAGCCAGTTACTTCAGATGTTCTTGAATATGATGAAGTTGTTGCTAAGTTTGAAAAGATGATGGATTGGTTA
GCAGATCTTTATGTTAATGTACTTAACCTTATCCATTATATGCATGATAAGTACTACTATGAAGCTGCTGAGATGGCTCT
TATCGATACAGATGTTAAGAGAACATTTGCAACAGGTATTGCAGGATTCTCACATGTAGTAGATTCACTTTCAGCTATTA
AGTATGCTAAGGTTACTGTATCAGAGAGAGATCCTGAGACAGGTATCGCTATGGCATTTAAGACAGAGGGAGACTTCCCT
AAGTATGGTAATGATGATGACAGAGCTGATGATATCGCTGTATGGCTTCTTAAGTCATTCCTTGACAAGATTAAGAAGAG
ACATACTTACAGAAATTCTGAGCCAACAACATCTATCCTTACAATTACATCTAATGTAGTATATGGTAAGTTTACAGGTA
ACATGCCAGATGGACGTAAGGCTGGTACACCACTTGCTCCAGGTGCAAACCCAAGCTACGGTGCAGAGCAGAACGGACTT
CTTGCTTCACTTAACTCTCTTACAAAGCTTCCATATGAGTGGGCTCTTGATGGTATCTCTAATACACAGACAATGAACCC
AGATGCTCTTGGACATGATGATTCAGAGAGAGTTAACAACCTTGTTAACGTAATGGACGGATACTTCGATCAGGGTGCTC
ATCACCTTAACGTTAACGTATTTGGTAAGGATAAGCTTCTTGATGCTATGGAGCATCCAGAAAAGCCAGAGTACGCTAAC
TTCACAATTCGTGTATCTGGATATGCTGTTAAGTTCATCGACCTTACAAAGGAACAGCAGATGGATGTTATCAGCAGAAC
ATTCCACGACAGAATGTAA

Upstream 100 bases:

>100_bases
ATTTTTATTTTGACTAAGATTGTATACAGTGTACAAAAAGAAATACATTTTTCTTAAAATGTACATTGAAATATATTATA
TTTAAGGAGGATCAATTCAA

Downstream 100 bases:

>100_bases
TTGCTGTTTTTGAGTTTTTTCAGATTTTATAATACAGTGTTTATGCGGGTTTGAGAGATTTGAAGAAAAATCAGTTTAAT
GTAAATTACCATAAAATAGT

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MVEKTQWAGFKGRLWKEEINVRDFIQNNYTPYDGDESFLAGPTEATNKLWGKLQELQKEERAKGGVLDMETKVVTGLTAY
GPGYIDESMKDLEQVVGLQTDKPLKRAFMPYGGIKMAEESCENYGYTPDPELHKVFTEYHKTHNQGVFDAYTPEMRAARR
SHIITGLPDTYGRGRIVGDYRRVALYGIDYLIKCKEEDKANCGCGVMTNDVIQLREELTDQINALKGMKAMAAAYGYDIS
EPATTAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYINKDLEAGKITEAEAQELIDHFVMKCRMVKFARITSYNE
LFSGDPTWATLEVGGTGIDGRSMVTKNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVM
KVTWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDMKSKVQVGPAYKPVTSDVLEYDEVVAKFEKMMDWL
ADLYVNVLNLIHYMHDKYYYEAAEMALIDTDVKRTFATGIAGFSHVVDSLSAIKYAKVTVSERDPETGIAMAFKTEGDFP
KYGNDDDRADDIAVWLLKSFLDKIKKRHTYRNSEPTTSILTITSNVVYGKFTGNMPDGRKAGTPLAPGANPSYGAEQNGL
LASLNSLTKLPYEWALDGISNTQTMNPDALGHDDSERVNNLVNVMDGYFDQGAHHLNVNVFGKDKLLDAMEHPEKPEYAN
FTIRVSGYAVKFIDLTKEQQMDVISRTFHDRM

Sequences:

>Translated_752_residues
MVEKTQWAGFKGRLWKEEINVRDFIQNNYTPYDGDESFLAGPTEATNKLWGKLQELQKEERAKGGVLDMETKVVTGLTAY
GPGYIDESMKDLEQVVGLQTDKPLKRAFMPYGGIKMAEESCENYGYTPDPELHKVFTEYHKTHNQGVFDAYTPEMRAARR
SHIITGLPDTYGRGRIVGDYRRVALYGIDYLIKCKEEDKANCGCGVMTNDVIQLREELTDQINALKGMKAMAAAYGYDIS
EPATTAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYINKDLEAGKITEAEAQELIDHFVMKCRMVKFARITSYNE
LFSGDPTWATLEVGGTGIDGRSMVTKNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVM
KVTWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDMKSKVQVGPAYKPVTSDVLEYDEVVAKFEKMMDWL
ADLYVNVLNLIHYMHDKYYYEAAEMALIDTDVKRTFATGIAGFSHVVDSLSAIKYAKVTVSERDPETGIAMAFKTEGDFP
KYGNDDDRADDIAVWLLKSFLDKIKKRHTYRNSEPTTSILTITSNVVYGKFTGNMPDGRKAGTPLAPGANPSYGAEQNGL
LASLNSLTKLPYEWALDGISNTQTMNPDALGHDDSERVNNLVNVMDGYFDQGAHHLNVNVFGKDKLLDAMEHPEKPEYAN
FTIRVSGYAVKFIDLTKEQQMDVISRTFHDRM
>Mature_752_residues
MVEKTQWAGFKGRLWKEEINVRDFIQNNYTPYDGDESFLAGPTEATNKLWGKLQELQKEERAKGGVLDMETKVVTGLTAY
GPGYIDESMKDLEQVVGLQTDKPLKRAFMPYGGIKMAEESCENYGYTPDPELHKVFTEYHKTHNQGVFDAYTPEMRAARR
SHIITGLPDTYGRGRIVGDYRRVALYGIDYLIKCKEEDKANCGCGVMTNDVIQLREELTDQINALKGMKAMAAAYGYDIS
EPATTAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYINKDLEAGKITEAEAQELIDHFVMKCRMVKFARITSYNE
LFSGDPTWATLEVGGTGIDGRSMVTKNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVM
KVTWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDMKSKVQVGPAYKPVTSDVLEYDEVVAKFEKMMDWL
ADLYVNVLNLIHYMHDKYYYEAAEMALIDTDVKRTFATGIAGFSHVVDSLSAIKYAKVTVSERDPETGIAMAFKTEGDFP
KYGNDDDRADDIAVWLLKSFLDKIKKRHTYRNSEPTTSILTITSNVVYGKFTGNMPDGRKAGTPLAPGANPSYGAEQNGL
LASLNSLTKLPYEWALDGISNTQTMNPDALGHDDSERVNNLVNVMDGYFDQGAHHLNVNVFGKDKLLDAMEHPEKPEYAN
FTIRVSGYAVKFIDLTKEQQMDVISRTFHDRM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=758, Percent_Identity=60.6860158311346, Blast_Score=936, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=752, Percent_Identity=58.6436170212766, Blast_Score=896, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=652, Percent_Identity=25.6134969325153, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI1790388, Length=727, Percent_Identity=23.1086657496561, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1788933, Length=62, Percent_Identity=58.0645161290323, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84327; Mature: 84327

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKTQWAGFKGRLWKEEINVRDFIQNNYTPYDGDESFLAGPTEATNKLWGKLQELQKEE
CCCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RAKGGVLDMETKVVTGLTAYGPGYIDESMKDLEQVVGLQTDKPLKRAFMPYGGIKMAEES
HHCCCCEEECHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHH
CENYGYTPDPELHKVFTEYHKTHNQGVFDAYTPEMRAARRSHIITGLPDTYGRGRIVGDY
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEECCCCCCCCCCEEHHH
RRVALYGIDYLIKCKEEDKANCGCGVMTNDVIQLREELTDQINALKGMKAMAAAYGYDIS
HEEHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EPATTAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYINKDLEAGKITEAEAQELI
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHEEEEEEECCCCCCCCCCHHHHHHHH
DHFVMKCRMVKFARITSYNELFSGDPTWATLEVGGTGIDGRSMVTKNDYRFLHTLENMGP
HHHHHHHHHHHHHHHHCHHHHHCCCCCEEEEEECCCCCCCCCCEECCCHHHHHHHHHCCC
SPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVMKVTWGDDYSICCCVSATQTG
CCCCCEEEEECCCCCHHHHHHHHEEEEEECEEEECCCCEEEEEECCCCCEEEEEECCCCC
KEMQFFGARANLAKCLLYAINGGVDMKSKVQVGPAYKPVTSDVLEYDEVVAKFEKMMDWL
CHHHHHCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ADLYVNVLNLIHYMHDKYYYEAAEMALIDTDVKRTFATGIAGFSHVVDSLSAIKYAKVTV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
SERDPETGIAMAFKTEGDFPKYGNDDDRADDIAVWLLKSFLDKIKKRHTYRNSEPTTSIL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
TITSNVVYGKFTGNMPDGRKAGTPLAPGANPSYGAEQNGLLASLNSLTKLPYEWALDGIS
EEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCC
NTQTMNPDALGHDDSERVNNLVNVMDGYFDQGAHHLNVNVFGKDKLLDAMEHPEKPEYAN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCCCC
FTIRVSGYAVKFIDLTKEQQMDVISRTFHDRM
EEEEEECEEEEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEKTQWAGFKGRLWKEEINVRDFIQNNYTPYDGDESFLAGPTEATNKLWGKLQELQKEE
CCCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RAKGGVLDMETKVVTGLTAYGPGYIDESMKDLEQVVGLQTDKPLKRAFMPYGGIKMAEES
HHCCCCEEECHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHH
CENYGYTPDPELHKVFTEYHKTHNQGVFDAYTPEMRAARRSHIITGLPDTYGRGRIVGDY
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEECCCCCCCCCCEEHHH
RRVALYGIDYLIKCKEEDKANCGCGVMTNDVIQLREELTDQINALKGMKAMAAAYGYDIS
HEEHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EPATTAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYINKDLEAGKITEAEAQELI
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHEEEEEEECCCCCCCCCCHHHHHHHH
DHFVMKCRMVKFARITSYNELFSGDPTWATLEVGGTGIDGRSMVTKNDYRFLHTLENMGP
HHHHHHHHHHHHHHHHCHHHHHCCCCCEEEEEECCCCCCCCCCEECCCHHHHHHHHHCCC
SPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVMKVTWGDDYSICCCVSATQTG
CCCCCEEEEECCCCCHHHHHHHHEEEEEECEEEECCCCEEEEEECCCCCEEEEEECCCCC
KEMQFFGARANLAKCLLYAINGGVDMKSKVQVGPAYKPVTSDVLEYDEVVAKFEKMMDWL
CHHHHHCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ADLYVNVLNLIHYMHDKYYYEAAEMALIDTDVKRTFATGIAGFSHVVDSLSAIKYAKVTV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
SERDPETGIAMAFKTEGDFPKYGNDDDRADDIAVWLLKSFLDKIKKRHTYRNSEPTTSIL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
TITSNVVYGKFTGNMPDGRKAGTPLAPGANPSYGAEQNGLLASLNSLTKLPYEWALDGIS
EEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCC
NTQTMNPDALGHDDSERVNNLVNVMDGYFDQGAHHLNVNVFGKDKLLDAMEHPEKPEYAN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCCCC
FTIRVSGYAVKFIDLTKEQQMDVISRTFHDRM
EEEEEECEEEEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA