Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 238921763

GI number: 238921763

Start: 3807818

End: 3809185

Strand: Reverse

Name: trmE [H]

Synonym: NT01EI_3932

Alternate gene names: 238921763

Gene position: 3809185-3807818 (Counterclockwise)

Preceding gene: 238921764

Following gene: 238921760

Centisome position: 99.92

GC content: 62.72

Gene sequence:

>1368_bases
ATGATCAGCTCATCCGACACCATCGTCGCCCAGGCTACCGCGCCGGGACGCGGCGGCGTAGGGATCCTGCGCGTATCCGG
CCCGCAGGCGCAGGCCGTAGCCATGGCGCTATTGGGCAAGCTGCCCAAACCGCGCTATGCCGATTACCTGCCGTTCCGAG
ACCATGATGGAACCCCGCTGGATCAGGGCATTGCCCTCTATTTCCCCGGCCCCAACTCCTTTACCGGAGAGGATGTTCTG
GAGCTACAGGGCCACGGTGGTCCGGTGATCCTCGATTTATTGTTAAAGCGTATTCTGCAACTGCCGGAACTGCGCATCGC
CCGTCCGGGTGAGTTTTCCGAACGTGCATTCCTCAACGATAAAATGGATCTGGCGCAGGCCGAGGCGATTGCCGACCTTA
TCGATGCCAGCTCAGAGCAGGCGGCTCGCTCGGCGGTCAACTCGCTGCAGGGCGTCTTCTCGGCGCGCATCCATCAGCTG
GTCGAGTCCCTGATCCACCTGCGTATCTACGTCGAGGCCGCCATCGACTTTCCCGATGAAGAGATAGACTTTCTCTCCGA
CGGTAAGGTGGAGGCTCATCTGAATGCGGTGATGGATGAACTGGCCGGCGTACGCACGGAAGCGCGCCAGGGCAGCCTGC
TGCGTGAGGGGATGAAAGTGGTGATCGCCGGGCGTCCTAACGCCGGGAAATCCAGTCTGCTCAATGCGCTGGCCGGACGA
GAGGCGGCTATCGTCACTGATATCGCCGGGACCACTCGCGACGTCCTGCGTGAGCATATTCATATCGATGGCATGCCGCT
GCACATCATCGATACCGCCGGGCTGCGCGAGGCCAGCGACGAGGTGGAGCGTATCGGCATTGAGCGCGCCTGGCAGGAGA
TCGAGCGCGCCGATCGCGTACTGTTTATGGTCGACGGCACCACCACCGACGCCACCGATCCCGCCGCCATCTGGCCCGAT
TTTATTGCGCGTCTACCAGCAAGCCTGCCCATTACCGTAGTGCGGAATAAGGCGGACGTCACCGGTGAGACGCTGGGGAT
CAGCGACTGCGATGGCCATACGCTGGTGCGCCTGTCGGCCCGCACCGGCGCTGGCGTCGACGTGCTGCGCGATCACCTCA
AGCGCAGTATGGGCTTTGACACCCGTCTGGAGGGGGGATTTCTGGCGCGCCGCCGTCACCTGCAGGCGCTGGATCAGGCC
GCCCTGCACCTGCAGCAGGGAAAAGATCAGCTGCTCGGCGCCATGGCCGGTGAGCTGCTAGCCGAAGAGCTGCGTCTGGC
GCAGCAGTCGCTCAGCGAGATCACCGGCGAATTTACCTCTGACGATCTGCTGGGGCGCATTTTCACCAGCTTCTGCATCG
GCAAGTGA

Upstream 100 bases:

>100_bases
CGCTGATGCAGCGAGGTACCAGGGGGGCGGTCTGATGACCGCCCCTTTTTTCTTGCCGCCCGCCGCGCGGGCCAGCACTC
AACGCCAAAAGAGAGAGAAC

Downstream 100 bases:

>100_bases
GGCTAGGCCGAAACGAACGCCGGCCCGCCCGGCGTTATCCCCGTCGTTTCTGGTGACGCTCTCGGTTATCCGCCTTACGC
CGCTCGCTCTTACGCAGCAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MISSSDTIVAQATAPGRGGVGILRVSGPQAQAVAMALLGKLPKPRYADYLPFRDHDGTPLDQGIALYFPGPNSFTGEDVL
ELQGHGGPVILDLLLKRILQLPELRIARPGEFSERAFLNDKMDLAQAEAIADLIDASSEQAARSAVNSLQGVFSARIHQL
VESLIHLRIYVEAAIDFPDEEIDFLSDGKVEAHLNAVMDELAGVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGR
EAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIERADRVLFMVDGTTTDATDPAAIWPD
FIARLPASLPITVVRNKADVTGETLGISDCDGHTLVRLSARTGAGVDVLRDHLKRSMGFDTRLEGGFLARRRHLQALDQA
ALHLQQGKDQLLGAMAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFTSFCIGK

Sequences:

>Translated_455_residues
MISSSDTIVAQATAPGRGGVGILRVSGPQAQAVAMALLGKLPKPRYADYLPFRDHDGTPLDQGIALYFPGPNSFTGEDVL
ELQGHGGPVILDLLLKRILQLPELRIARPGEFSERAFLNDKMDLAQAEAIADLIDASSEQAARSAVNSLQGVFSARIHQL
VESLIHLRIYVEAAIDFPDEEIDFLSDGKVEAHLNAVMDELAGVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGR
EAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIERADRVLFMVDGTTTDATDPAAIWPD
FIARLPASLPITVVRNKADVTGETLGISDCDGHTLVRLSARTGAGVDVLRDHLKRSMGFDTRLEGGFLARRRHLQALDQA
ALHLQQGKDQLLGAMAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFTSFCIGK
>Mature_455_residues
MISSSDTIVAQATAPGRGGVGILRVSGPQAQAVAMALLGKLPKPRYADYLPFRDHDGTPLDQGIALYFPGPNSFTGEDVL
ELQGHGGPVILDLLLKRILQLPELRIARPGEFSERAFLNDKMDLAQAEAIADLIDASSEQAARSAVNSLQGVFSARIHQL
VESLIHLRIYVEAAIDFPDEEIDFLSDGKVEAHLNAVMDELAGVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGR
EAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIERADRVLFMVDGTTTDATDPAAIWPD
FIARLPASLPITVVRNKADVTGETLGISDCDGHTLVRLSARTGAGVDVLRDHLKRSMGFDTRLEGGFLARRRHLQALDQA
ALHLQQGKDQLLGAMAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFTSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=471, Percent_Identity=36.0934182590234, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=34.1222879684418, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI193082993, Length=465, Percent_Identity=36.1290322580645, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=86.784140969163, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=40.6593406593407, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=28.6031042128603, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.7907444668008, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=33.0561330561331, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49202; Mature: 49202

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSSDTIVAQATAPGRGGVGILRVSGPQAQAVAMALLGKLPKPRYADYLPFRDHDGTPL
CCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DQGIALYFPGPNSFTGEDVLELQGHGGPVILDLLLKRILQLPELRIARPGEFSERAFLND
CCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCC
KMDLAQAEAIADLIDASSEQAARSAVNSLQGVFSARIHQLVESLIHLRIYVEAAIDFPDE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
EIDFLSDGKVEAHLNAVMDELAGVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCC
EAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIERADRV
CCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEE
LFMVDGTTTDATDPAAIWPDFIARLPASLPITVVRNKADVTGETLGISDCDGHTLVRLSA
EEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEC
RTGAGVDVLRDHLKRSMGFDTRLEGGFLARRRHLQALDQAALHLQQGKDQLLGAMAGELL
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AEELRLAQQSLSEITGEFTSDDLLGRIFTSFCIGK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISSSDTIVAQATAPGRGGVGILRVSGPQAQAVAMALLGKLPKPRYADYLPFRDHDGTPL
CCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DQGIALYFPGPNSFTGEDVLELQGHGGPVILDLLLKRILQLPELRIARPGEFSERAFLND
CCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCC
KMDLAQAEAIADLIDASSEQAARSAVNSLQGVFSARIHQLVESLIHLRIYVEAAIDFPDE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
EIDFLSDGKVEAHLNAVMDELAGVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCC
EAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIERADRV
CCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEE
LFMVDGTTTDATDPAAIWPDFIARLPASLPITVVRNKADVTGETLGISDCDGHTLVRLSA
EEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEC
RTGAGVDVLRDHLKRSMGFDTRLEGGFLARRRHLQALDQAALHLQQGKDQLLGAMAGELL
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AEELRLAQQSLSEITGEFTSDDLLGRIFTSFCIGK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA