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The map label for this gene is gidB

Identifier: 238921735

GI number: 238921735

Start: 3783095

End: 3783715

Strand: Direct

Name: gidB

Synonym: NT01EI_3904

Alternate gene names: 238921735

Gene position: 3783095-3783715 (Clockwise)

Preceding gene: 238921734

Following gene: 238921736

Centisome position: 99.23

GC content: 56.36

Gene sequence:

>621_bases
TTGTACAAGCAACTGGACACGCTGCTGGCGCAGGCCGGCATTTCGTTAACCGATCAGCAAAAACAACGGCTACTGGGCTA
CGTATCTCTGCTGGATAAGTGGAATAAGGCCTATAACCTGACCTCTGTACGCGAGCCGCGCGACATGCTGGTGCGCCATA
TCATGGACAGCATCGTTGTGAGTCCTTATCTGCAGGGGCTGCGGTTCATCGACGTGGGAACAGGACCGGGTCTGCCGGGG
ATCCCTCTGGCTATTGTGCGCCCTGATGCCAATTTCACGCTGCTGGATAGCTTAGGCAAGCGGGTACGTTTCCTGCGTCA
GGTCCAGCATGAGCTGCGTTTGGACAATATTGAGCCGGTGCAGAGCCGGGTCGAGGCGTTTGTCGCAGAGCCGCCTTTTG
ACGGTGTGATCAGCCGTGCTTTTGCCTCTTTACAGGATATGGTGTCTTGGTGTCATCATCTCCCAGCCGCGCACCATGGA
CGCTTTTATGCCCTGAAGGGGGGAGTTCCACAGGATGAGCTGCAAAGCTTACCGGCCGGCGTTAGCGTGGATCAACTGAT
CCGCCTGCAGGTGCCAATGCTGGAGGGGGAACGCCATCTGGTGGTACTAAAATCCGATTAA

Upstream 100 bases:

>100_bases
CGTCGCAGCCTGTAGCTGCGCGCCCATTGGCCGCTGCCTGGCGGCCATTATTCACGACCGGCCGTCTTTAAGACGGCCGG
CTTTGATTGAGGTATATACC

Downstream 100 bases:

>100_bases
TTTTGTTTTCTGTCAAAAAAACAAAAAAAAACATTGCATAGTAAGTGAACGGATCAACCGTTAATAACTTGAATCATTTG
TAGACATATATTTCATTTGT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MYKQLDTLLAQAGISLTDQQKQRLLGYVSLLDKWNKAYNLTSVREPRDMLVRHIMDSIVVSPYLQGLRFIDVGTGPGLPG
IPLAIVRPDANFTLLDSLGKRVRFLRQVQHELRLDNIEPVQSRVEAFVAEPPFDGVISRAFASLQDMVSWCHHLPAAHHG
RFYALKGGVPQDELQSLPAGVSVDQLIRLQVPMLEGERHLVVLKSD

Sequences:

>Translated_206_residues
MYKQLDTLLAQAGISLTDQQKQRLLGYVSLLDKWNKAYNLTSVREPRDMLVRHIMDSIVVSPYLQGLRFIDVGTGPGLPG
IPLAIVRPDANFTLLDSLGKRVRFLRQVQHELRLDNIEPVQSRVEAFVAEPPFDGVISRAFASLQDMVSWCHHLPAAHHG
RFYALKGGVPQDELQSLPAGVSVDQLIRLQVPMLEGERHLVVLKSD
>Mature_206_residues
MYKQLDTLLAQAGISLTDQQKQRLLGYVSLLDKWNKAYNLTSVREPRDMLVRHIMDSIVVSPYLQGLRFIDVGTGPGLPG
IPLAIVRPDANFTLLDSLGKRVRFLRQVQHELRLDNIEPVQSRVEAFVAEPPFDGVISRAFASLQDMVSWCHHLPAAHHG
RFYALKGGVPQDELQSLPAGVSVDQLIRLQVPMLEGERHLVVLKSD

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=206, Percent_Identity=73.7864077669903, Blast_Score=319, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_EDWI9 (C5BF32)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935250.1
- ProteinModelPortal:   C5BF32
- GeneID:   7961470
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3904
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23109; Mature: 23109

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKQLDTLLAQAGISLTDQQKQRLLGYVSLLDKWNKAYNLTSVREPRDMLVRHIMDSIVV
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SPYLQGLRFIDVGTGPGLPGIPLAIVRPDANFTLLDSLGKRVRFLRQVQHELRLDNIEPV
HHHHCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QSRVEAFVAEPPFDGVISRAFASLQDMVSWCHHLPAAHHGRFYALKGGVPQDELQSLPAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCC
VSVDQLIRLQVPMLEGERHLVVLKSD
CCHHHHHHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure
MYKQLDTLLAQAGISLTDQQKQRLLGYVSLLDKWNKAYNLTSVREPRDMLVRHIMDSIVV
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SPYLQGLRFIDVGTGPGLPGIPLAIVRPDANFTLLDSLGKRVRFLRQVQHELRLDNIEPV
HHHHCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QSRVEAFVAEPPFDGVISRAFASLQDMVSWCHHLPAAHHGRFYALKGGVPQDELQSLPAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCC
VSVDQLIRLQVPMLEGERHLVVLKSD
CCHHHHHHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA