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The map label for this gene is mnmG [H]

Identifier: 238921734

GI number: 238921734

Start: 3781120

End: 3783009

Strand: Direct

Name: mnmG [H]

Synonym: NT01EI_3903

Alternate gene names: 238921734

Gene position: 3781120-3783009 (Clockwise)

Preceding gene: 238921733

Following gene: 238921735

Centisome position: 99.18

GC content: 55.93

Gene sequence:

>1890_bases
ATGTTTTACCCCGATCCCTTTGACGTCATCATTATCGGTGGCGGCCATGCCGGTACCGAAGCGGCCATGGCTTCTGCCCG
AATGGGCCGCCAGACCCTGTTATTAACACATAATATCGATACGCTAGGACATATGTCCTGCAATCCAGCCATCGGTGGTA
TCGGTAAGGGGCACTTAGTAAAAGAGGTCGATGCCCTGGGGGGACTGATGGCTCAGGCGATCGACAAAGGTGGGATCCAG
TTTAGGATCCTCAACGGCAGCAAAGGACCCGCGGTACGTGCAACCCGGGCACAGGCCGATCGCATACTGTATCGTCAGGC
GGTACGTATGGCGCTAGAGAACCAGCCCAACCTGATGATCTTTCAACAGGCTGTAGAGGATCTGATCGTTGAAAACGATC
GCGTCGTCGGTGCGGTTACCCAAATGGGGCTAAAGTTCCGCGCCAAAGCTGTGGTTCTCACCGTAGGCACGTTCCTGAAC
GGTAAAATTCATATCGGTCTGGAGAACTACAGCGGTGGTCGCGCAGGCGATCCGCCTTCCATCGGACTGGCCCGCCGCCT
GCGCGAGTTACCGCTACGGGTAAACCGACTGAAAACCGGCACACCTCCGCGCCTTGACGCTCGAACCATCGACTTCAGCG
TGCTGGCGCAGCAGCACGGTGACAACCCAGCGCCGGTTTTTTCTTTCCTCGGTGAGGCCAGTCAGCATCCGCGTCAGGTA
CCGTGCTATATAACGCATACCAACGAACATACCCATGAGGTGATCCGCGGCAATCTGGATCGCAGCCCGATGTATGCCGG
GATCATCGAGGGGATCGGCCCACGCTATTGCCCGTCGATCGAAGATAAGGTGATGCGCTTTGCCGATCGTAATGCGCACC
AGATCTTTTTGGAGCCAGAGGGACTGAGCAGCAATGAGATCTATCCAAACGGGATCTCAACCAGCTTGCCGTTTGACGTT
CAGATGGCCATCGTACGGTCGATGGCGGGGATGCAAAACACCCGCATCGTGCGTCCGGGGTATGCGATTGAGTATGATTT
CTTCGATCCGCGGGACTTGAAGCCCACCCTGGAGAGTAAATACATTCAGGGGCTGTTCTTCGCCGGTCAGATCAACGGCA
CAACCGGCTATGAGGAAGCGGCGGCACAGGGGTTACTGGCAGGCCTGAACGCTGGACGCTGTGCTGCCGATTTAGAAGGT
TGGATGCCCCGTCGCGACGAGGCATACCTTGGCGTGCTGGTGGATGACCTGTGTACCCTGGGTACCAAAGAGCCCTACCG
CATGTTTACCTCACGTGCGGAGTATCGTCTGATGTTGCGTGAAGATAACGCCGACTTGCGTTTGACCCCGATAGGGCGTG
AACTGGGTCTGGTCGACGATGTGCGTTGGGCCCAGTTTAACCAGAAGCTGGAAAACATGGAGCGTGAACGTCAGCGCCTG
CGTGAAACCTGGGTACACCCGCAGTCTGAACGGGCGGCTGAGGTGAATGTGTTGTTAAAAACCCCGCTGTCTCGCGAGGC
CAGCGGTGAGGATCTATTGCGTAGGCCTGAGATGGATTATGCCACGCTGACCTCGCTGTCTCCGTTTTATCCACCGCTGG
CGGATCGTCAGGCGGCTGAGCAGGTTGAGATCCAAGTTAAATACCAAGGGTATATTGCCCGTCAGCAGGACGAAATTGAA
AAGCAGCTGCGTAACGAAAATACGTTGCTGCCAGCCGATTTGGACTATCGTCAGGTCAATGGCTTGTCCAATGAGGTGAT
CGCGAAACTTAACGATCATAAACCCAGCTCTATCGGTCAGGCATCGCGTATTTCCGGTATTACGCCTGCAGCGATCTCTA
TTCTGCTGGTGTGGCTGAAAAAGCAGGGAATGCTGCGTCGCAGCCTGTAG

Upstream 100 bases:

>100_bases
TCCACATAGGTTGGACCTGCGCCTAAACTTGAGTAGAATCCCCACCCCTAGGCAAATAACGATCGTTGAGAGCCTTTCGG
TCAGCGCGAGGTGTAGTACC

Downstream 100 bases:

>100_bases
CTGCGCGCCCATTGGCCGCTGCCTGGCGGCCATTATTCACGACCGGCCGTCTTTAAGACGGCCGGCTTTGATTGAGGTAT
ATACCTTGTACAAGCAACTG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYPDPFDVIIIGGGHAGTEAAMASARMGRQTLLLTHNIDTLGHMSCNPAIGGIGKGHLVKEVDALGGLMAQAIDKGGIQ
FRILNGSKGPAVRATRAQADRILYRQAVRMALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLN
GKIHIGLENYSGGRAGDPPSIGLARRLRELPLRVNRLKTGTPPRLDARTIDFSVLAQQHGDNPAPVFSFLGEASQHPRQV
PCYITHTNEHTHEVIRGNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPEGLSSNEIYPNGISTSLPFDV
QMAIVRSMAGMQNTRIVRPGYAIEYDFFDPRDLKPTLESKYIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRCAADLEG
WMPRRDEAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTPIGRELGLVDDVRWAQFNQKLENMERERQRL
RETWVHPQSERAAEVNVLLKTPLSREASGEDLLRRPEMDYATLTSLSPFYPPLADRQAAEQVEIQVKYQGYIARQQDEIE
KQLRNENTLLPADLDYRQVNGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGMLRRSL

Sequences:

>Translated_629_residues
MFYPDPFDVIIIGGGHAGTEAAMASARMGRQTLLLTHNIDTLGHMSCNPAIGGIGKGHLVKEVDALGGLMAQAIDKGGIQ
FRILNGSKGPAVRATRAQADRILYRQAVRMALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLN
GKIHIGLENYSGGRAGDPPSIGLARRLRELPLRVNRLKTGTPPRLDARTIDFSVLAQQHGDNPAPVFSFLGEASQHPRQV
PCYITHTNEHTHEVIRGNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPEGLSSNEIYPNGISTSLPFDV
QMAIVRSMAGMQNTRIVRPGYAIEYDFFDPRDLKPTLESKYIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRCAADLEG
WMPRRDEAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTPIGRELGLVDDVRWAQFNQKLENMERERQRL
RETWVHPQSERAAEVNVLLKTPLSREASGEDLLRRPEMDYATLTSLSPFYPPLADRQAAEQVEIQVKYQGYIARQQDEIE
KQLRNENTLLPADLDYRQVNGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGMLRRSL
>Mature_629_residues
MFYPDPFDVIIIGGGHAGTEAAMASARMGRQTLLLTHNIDTLGHMSCNPAIGGIGKGHLVKEVDALGGLMAQAIDKGGIQ
FRILNGSKGPAVRATRAQADRILYRQAVRMALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLN
GKIHIGLENYSGGRAGDPPSIGLARRLRELPLRVNRLKTGTPPRLDARTIDFSVLAQQHGDNPAPVFSFLGEASQHPRQV
PCYITHTNEHTHEVIRGNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPEGLSSNEIYPNGISTSLPFDV
QMAIVRSMAGMQNTRIVRPGYAIEYDFFDPRDLKPTLESKYIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRCAADLEG
WMPRRDEAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTPIGRELGLVDDVRWAQFNQKLENMERERQRL
RETWVHPQSERAAEVNVLLKTPLSREASGEDLLRRPEMDYATLTSLSPFYPPLADRQAAEQVEIQVKYQGYIARQQDEIE
KQLRNENTLLPADLDYRQVNGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGMLRRSL

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=46.2025316455696, Blast_Score=515, Evalue=1e-146,
Organism=Homo sapiens, GI19882217, Length=657, Percent_Identity=44.4444444444444, Blast_Score=501, Evalue=1e-141,
Organism=Homo sapiens, GI183227703, Length=672, Percent_Identity=43.452380952381, Blast_Score=496, Evalue=1e-140,
Organism=Escherichia coli, GI2367273, Length=628, Percent_Identity=89.0127388535032, Blast_Score=1157, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=636, Percent_Identity=41.3522012578616, Blast_Score=465, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321202, Length=621, Percent_Identity=46.0547504025765, Blast_Score=519, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24658174, Length=634, Percent_Identity=47.0031545741325, Blast_Score=521, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69963; Mature: 69963

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS50096 IQ ; PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPDPFDVIIIGGGHAGTEAAMASARMGRQTLLLTHNIDTLGHMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHH
KEVDALGGLMAQAIDKGGIQFRILNGSKGPAVRATRAQADRILYRQAVRMALENQPNLMI
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLNGKIHIGLENYSGGRAGDPPS
HHHHHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEECEECCEEEEEEECCCCCCCCCCCC
IGLARRLRELPLRVNRLKTGTPPRLDARTIDFSVLAQQHGDNPAPVFSFLGEASQHPRQV
HHHHHHHHHCCCHHHHCCCCCCCCCCCEECHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
PCYITHTNEHTHEVIRGNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPE
CEEEECCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCC
GLSSNEIYPNGISTSLPFDVQMAIVRSMAGMQNTRIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCHHHHH
YIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRCAADLEGWMPRRDEAYLGVLVDDLCTL
HHCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTPIGRELGLVDDVRWAQFNQKLENMERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RETWVHPQSERAAEVNVLLKTPLSREASGEDLLRRPEMDYATLTSLSPFYPPLADRQAAE
HHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCC
QVEIQVKYQGYIARQQDEIEKQLRNENTLLPADLDYRQVNGLSNEVIAKLNDHKPSSIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLVWLKKQGMLRRSL
HHHCCCCCHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MFYPDPFDVIIIGGGHAGTEAAMASARMGRQTLLLTHNIDTLGHMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHH
KEVDALGGLMAQAIDKGGIQFRILNGSKGPAVRATRAQADRILYRQAVRMALENQPNLMI
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLNGKIHIGLENYSGGRAGDPPS
HHHHHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEECEECCEEEEEEECCCCCCCCCCCC
IGLARRLRELPLRVNRLKTGTPPRLDARTIDFSVLAQQHGDNPAPVFSFLGEASQHPRQV
HHHHHHHHHCCCHHHHCCCCCCCCCCCEECHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
PCYITHTNEHTHEVIRGNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPE
CEEEECCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCC
GLSSNEIYPNGISTSLPFDVQMAIVRSMAGMQNTRIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCHHHHH
YIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRCAADLEGWMPRRDEAYLGVLVDDLCTL
HHCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTPIGRELGLVDDVRWAQFNQKLENMERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RETWVHPQSERAAEVNVLLKTPLSREASGEDLLRRPEMDYATLTSLSPFYPPLADRQAAE
HHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCC
QVEIQVKYQGYIARQQDEIEKQLRNENTLLPADLDYRQVNGLSNEVIAKLNDHKPSSIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLVWLKKQGMLRRSL
HHHCCCCCHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA