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The map label for this gene is glnG [H]

Identifier: 238921704

GI number: 238921704

Start: 3744493

End: 3745908

Strand: Direct

Name: glnG [H]

Synonym: NT01EI_3865

Alternate gene names: 238921704

Gene position: 3744493-3745908 (Clockwise)

Preceding gene: 238921703

Following gene: 238921705

Centisome position: 98.22

GC content: 62.01

Gene sequence:

>1416_bases
ATGCAACCGGGTCTCGTCTGGATCGTCGACGATGACAGTGCCATCCGCTGGGTCTTAGAGCGCGCGCTGACCGGCGCAGG
TTTACGCTGCGCCACATTTGAAAATGGTCAGGCGGTTCTGACGGCGATGAGCGCCCAGACGCCGGACGTTCTACTGTCAG
ACATCCGCATGCCCGGTATGGACGGGCTGGCCCTGCTACAGCAGATAAAAATGCGCCATCCGTTGCTTCCGGTCATCATT
ATGACGGCCCATTCCGATCTGGATGCCGCGGTCAGCGCCTACCAGCAGGGCGCCTTTGATTATCTGCCCAAACCGTTCGA
TATTGACGAAGCCGTCGCGCTCGTCGAACGCGCTATCAGCCACTATCAGGAGCAGCAGCAGCCAGCACGCCAGACGCCAG
GAAGCGATCCCAGCGCCGATATTATTGGCGAAGCAGCGGCGATGCAGGATGTCTTTCGCATTATCGGGCGCCTGTCACGC
TCCTCCATCAGCGTGCTGATCAATGGGGAGTCGGGCACCGGAAAAGAGCTGGTCGCCCATGCGTTGCACCGCCACAGCCC
GCGTGCCAAGGGGCCATTTATTGCCCTGAACATGGCGGCCATTCCGCGCGATCTGATCGAATCCGAGCTGTTCGGCCATG
AAAAAGGCGCCTTTACCGGTGCCAGCCAGATCCGCCAGGGACGCTTTGAGCAAGCGGACGGCGGGACACTATTCCTCGAT
GAAATCGGCGACATGCCGTTGGACGTTCAGACCCGCCTGCTGCGCGTACTGGCCGACGGACAGTTTTATCGCATCGGTGG
CTATGCGCCGGTCAGGGTCGATGTGCGCATCGTCGCCGCGACCCATCAGAATCTGGAACAGCGGGTACAAGAGGGGAAGT
TCCGCGAAGATCTCTTCCACCGCCTCAACGTTATCCGCATACATCTTCCGCCGCTGCGTGAGCGGCGCGAGGATATTCCC
CGCCTGGCTCGCCATTTTCTCAAAGTGGCCGCACAGGAGCTGGGCGTCGAGGCCAAAGTGCTCAGCCCGGAGGCCGAAGC
GGCTCTGATCCGTCTACCGTGGCCTGGCAATGTGCGCCAGCTGGAGAATACCTGCCGCTGGCTGACGGTCATGGCCGCAG
GCCAAGAGGTGTTGACGCAGGATCTGCCGGCGGAGCTGTTTGAGCGCGCGCGCGATGAACGCCAGCCGGTGATACTCCCG
CCGGACAGCTGGCCACGGCTGCTGAGTCAGTGGGCGGAGCATGCCCTGCAGAGCGGCCAGCAGGATCTGCTGGCCGAAGC
CCTGCCGGAAATGGAGCGTACGCTGCTGCACTGCGCCCTGCACCACACTCATGGCCATAAACAAGAGGCGGCCAGGCTGC
TGGGATGGGGCCGCAATACCCTGACCCGCAAGCTGAAAGAGCTGGGAATGGAGTAA

Upstream 100 bases:

>100_bases
AATCGAGTTTAACAGCTGGCCGGGCCACACCGAGTTTTCGGTATATTTGCCAATCCGCAAATAGCCCACGCCCCTCACGC
AACAGGTGGTCACCATGACA

Downstream 100 bases:

>100_bases
TGATGGGGAGAGGACACCCTCGGTTAAAAACGAGGCCGTGCGCACAAAAAAACGGCAGTCGCGATCGTTATACCACCACG
CGGGAGAATAATGATGATCG

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MQPGLVWIVDDDSAIRWVLERALTGAGLRCATFENGQAVLTAMSAQTPDVLLSDIRMPGMDGLALLQQIKMRHPLLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARQTPGSDPSADIIGEAAAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKGPFIALNMAAIPRDLIESELFGHEKGAFTGASQIRQGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRIGGYAPVRVDVRIVAATHQNLEQRVQEGKFREDLFHRLNVIRIHLPPLRERREDIP
RLARHFLKVAAQELGVEAKVLSPEAEAALIRLPWPGNVRQLENTCRWLTVMAAGQEVLTQDLPAELFERARDERQPVILP
PDSWPRLLSQWAEHALQSGQQDLLAEALPEMERTLLHCALHHTHGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_471_residues
MQPGLVWIVDDDSAIRWVLERALTGAGLRCATFENGQAVLTAMSAQTPDVLLSDIRMPGMDGLALLQQIKMRHPLLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARQTPGSDPSADIIGEAAAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKGPFIALNMAAIPRDLIESELFGHEKGAFTGASQIRQGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRIGGYAPVRVDVRIVAATHQNLEQRVQEGKFREDLFHRLNVIRIHLPPLRERREDIP
RLARHFLKVAAQELGVEAKVLSPEAEAALIRLPWPGNVRQLENTCRWLTVMAAGQEVLTQDLPAELFERARDERQPVILP
PDSWPRLLSQWAEHALQSGQQDLLAEALPEMERTLLHCALHHTHGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_471_residues
MQPGLVWIVDDDSAIRWVLERALTGAGLRCATFENGQAVLTAMSAQTPDVLLSDIRMPGMDGLALLQQIKMRHPLLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARQTPGSDPSADIIGEAAAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKGPFIALNMAAIPRDLIESELFGHEKGAFTGASQIRQGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRIGGYAPVRVDVRIVAATHQNLEQRVQEGKFREDLFHRLNVIRIHLPPLRERREDIP
RLARHFLKVAAQELGVEAKVLSPEAEAALIRLPWPGNVRQLENTCRWLTVMAAGQEVLTQDLPAELFERARDERQPVILP
PDSWPRLLSQWAEHALQSGQQDLLAEALPEMERTLLHCALHHTHGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=85.1380042462845, Blast_Score=815, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=42.4307036247335, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=42.6406926406926, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=37.9828326180258, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=44.3768996960486, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI87082117, Length=354, Percent_Identity=42.090395480226, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789087, Length=336, Percent_Identity=41.3690476190476, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786524, Length=348, Percent_Identity=42.5287356321839, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=335, Percent_Identity=41.1940298507463, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787583, Length=360, Percent_Identity=37.2222222222222, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=38.2978723404255, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=31.2101910828025, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=36.6255144032922, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790860, Length=129, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786911, Length=136, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789809, Length=178, Percent_Identity=26.4044943820225, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=128, Percent_Identity=29.6875, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52511; Mature: 52511

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPGLVWIVDDDSAIRWVLERALTGAGLRCATFENGQAVLTAMSAQTPDVLLSDIRMPGM
CCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCHHHHHHHCCCCCC
DGLALLQQIKMRHPLLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPARQTPGSDPSADIIGEAAAMQDVFRIIGRLSRSSISVLINGESGTGKELVAH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAKGPFIALNMAAIPRDLIESELFGHEKGAFTGASQIRQGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRIGGYAPVRVDVRIVAATHQNLEQRVQEGKFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHH
RLNVIRIHLPPLRERREDIPRLARHFLKVAAQELGVEAKVLSPEAEAALIRLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCHHH
LENTCRWLTVMAAGQEVLTQDLPAELFERARDERQPVILPPDSWPRLLSQWAEHALQSGQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCH
QDLLAEALPEMERTLLHCALHHTHGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPGLVWIVDDDSAIRWVLERALTGAGLRCATFENGQAVLTAMSAQTPDVLLSDIRMPGM
CCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCHHHHHHHCCCCCC
DGLALLQQIKMRHPLLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPARQTPGSDPSADIIGEAAAMQDVFRIIGRLSRSSISVLINGESGTGKELVAH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAKGPFIALNMAAIPRDLIESELFGHEKGAFTGASQIRQGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRIGGYAPVRVDVRIVAATHQNLEQRVQEGKFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHH
RLNVIRIHLPPLRERREDIPRLARHFLKVAAQELGVEAKVLSPEAEAALIRLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCHHH
LENTCRWLTVMAAGQEVLTQDLPAELFERARDERQPVILPPDSWPRLLSQWAEHALQSGQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCH
QDLLAEALPEMERTLLHCALHHTHGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]