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The map label for this gene is metL [H]

Identifier: 238921651

GI number: 238921651

Start: 3686592

End: 3689030

Strand: Direct

Name: metL [H]

Synonym: NT01EI_3806

Alternate gene names: 238921651

Gene position: 3686592-3689030 (Clockwise)

Preceding gene: 238921650

Following gene: 238921655

Centisome position: 96.7

GC content: 61.46

Gene sequence:

>2439_bases
ATGAGTGCACAAGCGGTAACGGGGCAGCAGCAGGCAGATCGCCAACTGCATAAGTTCGGCGGCAGTAGCCTGGCAGACGT
AAAGTGTTATTTGCGCGTCGCGGGGATCATGGCGGAATACAGCCGCCCCGGCGACATGATGGTCGTGTCGGCGGCGGGCA
GCACCACTAATCAGCTGATCAGCTGGCTCAAGCTCAGCCAGAGCGATCGCATCTCTGCCCATCAGGTACAGCAAGCCCTG
CGCCGTTACCAGAGCGAGCTGATTGTCGGCTTGCTGCCGCCGGAGATGGCGGAGCCGCTGCAGACGCACTTTATTCGGGA
TCTTGAACGTCTGGCTGCGCTGCTGGATGGTGAGATGGATGAGGCGGCCTATGCCGAGGTGGTGGGCCATGGTGAGGTTT
GGTCCGCCCGGCTGATGGCGGCGGTGCTTCAGAGCCAGGGCCTGGAGGCGACATGGCTGGATGCACGGGATTTCCTGTGC
GCCGAGCACGCAGCCCAGCCACAGGTGGATGAAAGGCGCTCCTATCCACTGCTGCAGCAGCTGCTGGCCCAGCATCCGAA
TAAACGTCTGGTGGTAACCGGCTTTATCAGTCGCAGCGGTGCGGGTGAAACGGTATTGCTTGGCCGTAACGGCTCTGACT
ATTCAGCGACACAGATCGGTGCTCTGGCCGGCGTGCGGCGCGTGACTATCTGGAGCGATGTCGCCGGGGTTTACAGTGCC
GATCCGCGTAAGGTGAAAGATGCCTGTCTACTGCCGCTGCTGCGTCTGGATGAGGCCAGCGAGCTGGCTCGCCTGGCTGC
ACCGGTGCTGCATGCCCGCACGCTGCAGCCGGTGTCCGCCAGCGATATCGATCTGCAGCTGCGCTGCAGCTATCAGCCGC
AGCAGGGGTCAACCCGCATTGAGCGGGTGCTGGCCAGCGGTAACGGCGCCAAGATTGTCACCAGCCATGACGATGTCTGC
CTGATTGAGCTGCACGTTACCACTGGCCATGAGTTCAGTCATATACACCAAGAGGTAGAGCGTATTCTGACGCGCAGCCA
GCTGCGTCCGCTGGCCAGTGGGATCCATCGCGATCGTGGATTGATTCAGTTGTGCTACACAGCAGAAGTGGTCGGCAGTG
TATTTAAGCTGCTGGAGGAGTCGGCGATCCCGGGTCTGCTACAGCTACGCGAAGGTTTGGCGCTGGTGGCGATGGTCGGC
GCCGGCGTATGCCGTAATCCACTGCATGCTCATCGTTTTTATCAGCAGCTTAAGGATCAGCCTGTCGAGTTTATCTGGCA
GTCGGAAGAGAGCATCAGCCTGGTGGCGGTGTTACGCTGCGGCCCGACGGAGGCGCTGGTGCAGGGGCTACACAACTCGC
TGTTTCGGGCAGAAAAACGCGTCGGCCTGGTTCTGTTTGGTAAGGGTAATATCGGTTCCCGCTGGCTGGAGTTGTTCGCG
CGTGAACAGGGTAACCTGTCGGCGAGCAGTGGTTTCGAGTTTATTCTGACCGGGGTGGTCGACAGCAATCGCAGCCTGCT
GAACTATGATGGGCTGGACGCCAGCCGAGCGCTGGCCTTCTTCGACGATGAAGCGCAGGTGCTGGATGAGGAGTCGCTGT
TCCTGTGGATGCGAGCTCACCCCTATGATGATTTGGTGGTGCTCGATGTGACAGCCAGTCCCTCGCTGGCCCAGCAGTAC
CGCGATTTTGCCAGCTATGGCTTTCATATGGTCAGCGCCAACAAGCTGGCCGGGGCCGCCGGCGGCCACGAATACCGCCA
GATCCGTGATGCTTTTGAGAAAACCGGACGTCACTGGCTGTATAACGCGACCGTGGGCGCCGGGCTGCCGGTTAACTATA
CCGTGCGCGATCTGCGCGACAGTGGCGATACGATTCTGTCCATCAGCGGCATTTTCTCCGGCACCCTCTCCTGGCTGTTT
CTGCAGTTTGACGGCTCGATTCCCTTCAGCGCGCTGGTGGACCAGGCCTGGCAGCAGGGGCTGACGGAGCCCGACCCGCG
GGTGGACCTTTCCGGCCAGGACGTGATGCGCAAGCTGGTGATCCTGGCGCGTGAGGCGGGATATGACATTGAGCCTGCCC
AGGTCCGGGTCGAGTCCCTGGTGCCGGCCGGCGGCGAGCAGGGCTCTATCGATCACTTTTTTGAGAACAGCGAAGCGCTG
GACGAGCAGATGCTGCAGCGTCTGGCGGCGGCCAATGAGATGGGGCTGGTGCTGCGCTACGTGGCGCGCTTTGATGCCAA
CGGTAAGGCACGTGTTGGCGTTGAGGCCGTACGCCCGGAGCACCCATTGGCGTCACTGTTGCCGTGCGACAACGTGTTCG
CGATTGAAAGCCGCTGGTATCGCGATAACCCGCTGGTGATCCGCGGCCCAGGCGCGGGGCGCGATGTTACCGCCGGTGCG
ATTCAGTCCGATCTGAACCGTCTGGCGCGTCTGCTTTAG

Upstream 100 bases:

>100_bases
TAGCGACAGCCTGCTGCGTATTTCCGTGGGTATTGAGGACGGTGATGATTTAATCGCTGATCTGGAACAGGCATTTCAGG
CAGCAGCAACGAGGTAAGCG

Downstream 100 bases:

>100_bases
AATAATGTACGCCCGGGGCGCCGGGCAGGGATTTCTCCTTGCGCCCGGCCCCCGGTTGTGGGGTATACTCCCGCCGCATC
AATTGGAAGATCATCGTCCC

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 812; Mature: 811

Protein sequence:

>812_residues
MSAQAVTGQQQADRQLHKFGGSSLADVKCYLRVAGIMAEYSRPGDMMVVSAAGSTTNQLISWLKLSQSDRISAHQVQQAL
RRYQSELIVGLLPPEMAEPLQTHFIRDLERLAALLDGEMDEAAYAEVVGHGEVWSARLMAAVLQSQGLEATWLDARDFLC
AEHAAQPQVDERRSYPLLQQLLAQHPNKRLVVTGFISRSGAGETVLLGRNGSDYSATQIGALAGVRRVTIWSDVAGVYSA
DPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSASDIDLQLRCSYQPQQGSTRIERVLASGNGAKIVTSHDDVC
LIELHVTTGHEFSHIHQEVERILTRSQLRPLASGIHRDRGLIQLCYTAEVVGSVFKLLEESAIPGLLQLREGLALVAMVG
AGVCRNPLHAHRFYQQLKDQPVEFIWQSEESISLVAVLRCGPTEALVQGLHNSLFRAEKRVGLVLFGKGNIGSRWLELFA
REQGNLSASSGFEFILTGVVDSNRSLLNYDGLDASRALAFFDDEAQVLDEESLFLWMRAHPYDDLVVLDVTASPSLAQQY
RDFASYGFHMVSANKLAGAAGGHEYRQIRDAFEKTGRHWLYNATVGAGLPVNYTVRDLRDSGDTILSISGIFSGTLSWLF
LQFDGSIPFSALVDQAWQQGLTEPDPRVDLSGQDVMRKLVILAREAGYDIEPAQVRVESLVPAGGEQGSIDHFFENSEAL
DEQMLQRLAAANEMGLVLRYVARFDANGKARVGVEAVRPEHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
IQSDLNRLARLL

Sequences:

>Translated_812_residues
MSAQAVTGQQQADRQLHKFGGSSLADVKCYLRVAGIMAEYSRPGDMMVVSAAGSTTNQLISWLKLSQSDRISAHQVQQAL
RRYQSELIVGLLPPEMAEPLQTHFIRDLERLAALLDGEMDEAAYAEVVGHGEVWSARLMAAVLQSQGLEATWLDARDFLC
AEHAAQPQVDERRSYPLLQQLLAQHPNKRLVVTGFISRSGAGETVLLGRNGSDYSATQIGALAGVRRVTIWSDVAGVYSA
DPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSASDIDLQLRCSYQPQQGSTRIERVLASGNGAKIVTSHDDVC
LIELHVTTGHEFSHIHQEVERILTRSQLRPLASGIHRDRGLIQLCYTAEVVGSVFKLLEESAIPGLLQLREGLALVAMVG
AGVCRNPLHAHRFYQQLKDQPVEFIWQSEESISLVAVLRCGPTEALVQGLHNSLFRAEKRVGLVLFGKGNIGSRWLELFA
REQGNLSASSGFEFILTGVVDSNRSLLNYDGLDASRALAFFDDEAQVLDEESLFLWMRAHPYDDLVVLDVTASPSLAQQY
RDFASYGFHMVSANKLAGAAGGHEYRQIRDAFEKTGRHWLYNATVGAGLPVNYTVRDLRDSGDTILSISGIFSGTLSWLF
LQFDGSIPFSALVDQAWQQGLTEPDPRVDLSGQDVMRKLVILAREAGYDIEPAQVRVESLVPAGGEQGSIDHFFENSEAL
DEQMLQRLAAANEMGLVLRYVARFDANGKARVGVEAVRPEHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
IQSDLNRLARLL
>Mature_811_residues
SAQAVTGQQQADRQLHKFGGSSLADVKCYLRVAGIMAEYSRPGDMMVVSAAGSTTNQLISWLKLSQSDRISAHQVQQALR
RYQSELIVGLLPPEMAEPLQTHFIRDLERLAALLDGEMDEAAYAEVVGHGEVWSARLMAAVLQSQGLEATWLDARDFLCA
EHAAQPQVDERRSYPLLQQLLAQHPNKRLVVTGFISRSGAGETVLLGRNGSDYSATQIGALAGVRRVTIWSDVAGVYSAD
PRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSASDIDLQLRCSYQPQQGSTRIERVLASGNGAKIVTSHDDVCL
IELHVTTGHEFSHIHQEVERILTRSQLRPLASGIHRDRGLIQLCYTAEVVGSVFKLLEESAIPGLLQLREGLALVAMVGA
GVCRNPLHAHRFYQQLKDQPVEFIWQSEESISLVAVLRCGPTEALVQGLHNSLFRAEKRVGLVLFGKGNIGSRWLELFAR
EQGNLSASSGFEFILTGVVDSNRSLLNYDGLDASRALAFFDDEAQVLDEESLFLWMRAHPYDDLVVLDVTASPSLAQQYR
DFASYGFHMVSANKLAGAAGGHEYRQIRDAFEKTGRHWLYNATVGAGLPVNYTVRDLRDSGDTILSISGIFSGTLSWLFL
QFDGSIPFSALVDQAWQQGLTEPDPRVDLSGQDVMRKLVILAREAGYDIEPAQVRVESLVPAGGEQGSIDHFFENSEALD
EQMLQRLAAANEMGLVLRYVARFDANGKARVGVEAVRPEHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAI
QSDLNRLARLL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=799, Percent_Identity=81.9774718397997, Blast_Score=1340, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=819, Percent_Identity=31.3797313797314, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1790455, Length=459, Percent_Identity=27.0152505446623, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322599, Length=346, Percent_Identity=33.2369942196532, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320893, Length=501, Percent_Identity=21.5568862275449, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 89213; Mature: 89082

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQAVTGQQQADRQLHKFGGSSLADVKCYLRVAGIMAEYSRPGDMMVVSAAGSTTNQLI
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
SWLKLSQSDRISAHQVQQALRRYQSELIVGLLPPEMAEPLQTHFIRDLERLAALLDGEMD
HHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAYAEVVGHGEVWSARLMAAVLQSQGLEATWLDARDFLCAEHAAQPQVDERRSYPLLQQ
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCHHHCCCHHHHH
LLAQHPNKRLVVTGFISRSGAGETVLLGRNGSDYSATQIGALAGVRRVTIWSDVAGVYSA
HHHHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEHHHHHHHCCC
DPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSASDIDLQLRCSYQPQQGSTRI
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHH
ERVLASGNGAKIVTSHDDVCLIELHVTTGHEFSHIHQEVERILTRSQLRPLASGIHRDRG
HHHHHCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIQLCYTAEVVGSVFKLLEESAIPGLLQLREGLALVAMVGAGVCRNPLHAHRFYQQLKDQ
CEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCC
PVEFIWQSEESISLVAVLRCGPTEALVQGLHNSLFRAEKRVGLVLFGKGNIGSRWLELFA
CHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
REQGNLSASSGFEFILTGVVDSNRSLLNYDGLDASRALAFFDDEAQVLDEESLFLWMRAH
HHCCCCCCCCCCEEEEEEEECCCCCEEEECCCCHHCEEEEECCCHHHCCCCCEEEEEECC
PYDDLVVLDVTASPSLAQQYRDFASYGFHMVSANKLAGAAGGHEYRQIRDAFEKTGRHWL
CCCCEEEEEECCCCHHHHHHHHHHHCCEEEEECCHHCCCCCCHHHHHHHHHHHHCCCEEE
YNATVGAGLPVNYTVRDLRDSGDTILSISGIFSGTLSWLFLQFDGSIPFSALVDQAWQQG
EEECCCCCCCCCCCHHHHCCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCC
LTEPDPRVDLSGQDVMRKLVILAREAGYDIEPAQVRVESLVPAGGEQGSIDHFFENSEAL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCHHHH
DEQMLQRLAAANEMGLVLRYVARFDANGKARVGVEAVRPEHPLASLLPCDNVFAIESRWY
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECCEECCCCCCHHHHCCCCCEEEECCCEE
RDNPLVIRGPGAGRDVTAGAIQSDLNRLARLL
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SAQAVTGQQQADRQLHKFGGSSLADVKCYLRVAGIMAEYSRPGDMMVVSAAGSTTNQLI
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
SWLKLSQSDRISAHQVQQALRRYQSELIVGLLPPEMAEPLQTHFIRDLERLAALLDGEMD
HHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAYAEVVGHGEVWSARLMAAVLQSQGLEATWLDARDFLCAEHAAQPQVDERRSYPLLQQ
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCHHHCCCHHHHH
LLAQHPNKRLVVTGFISRSGAGETVLLGRNGSDYSATQIGALAGVRRVTIWSDVAGVYSA
HHHHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEHHHHHHHCCC
DPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSASDIDLQLRCSYQPQQGSTRI
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHH
ERVLASGNGAKIVTSHDDVCLIELHVTTGHEFSHIHQEVERILTRSQLRPLASGIHRDRG
HHHHHCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LIQLCYTAEVVGSVFKLLEESAIPGLLQLREGLALVAMVGAGVCRNPLHAHRFYQQLKDQ
CEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCC
PVEFIWQSEESISLVAVLRCGPTEALVQGLHNSLFRAEKRVGLVLFGKGNIGSRWLELFA
CHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
REQGNLSASSGFEFILTGVVDSNRSLLNYDGLDASRALAFFDDEAQVLDEESLFLWMRAH
HHCCCCCCCCCCEEEEEEEECCCCCEEEECCCCHHCEEEEECCCHHHCCCCCEEEEEECC
PYDDLVVLDVTASPSLAQQYRDFASYGFHMVSANKLAGAAGGHEYRQIRDAFEKTGRHWL
CCCCEEEEEECCCCHHHHHHHHHHHCCEEEEECCHHCCCCCCHHHHHHHHHHHHCCCEEE
YNATVGAGLPVNYTVRDLRDSGDTILSISGIFSGTLSWLFLQFDGSIPFSALVDQAWQQG
EEECCCCCCCCCCCHHHHCCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCC
LTEPDPRVDLSGQDVMRKLVILAREAGYDIEPAQVRVESLVPAGGEQGSIDHFFENSEAL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCHHHH
DEQMLQRLAAANEMGLVLRYVARFDANGKARVGVEAVRPEHPLASLLPCDNVFAIESRWY
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECCEECCCCCCHHHHCCCCCEEEECCCEE
RDNPLVIRGPGAGRDVTAGAIQSDLNRLARLL
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]