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The map label for this gene is priA [H]

Identifier: 238921647

GI number: 238921647

Start: 3682099

End: 3684294

Strand: Reverse

Name: priA [H]

Synonym: NT01EI_3802

Alternate gene names: 238921647

Gene position: 3684294-3682099 (Counterclockwise)

Preceding gene: 238921649

Following gene: 238921646

Centisome position: 96.64

GC content: 63.8

Gene sequence:

>2196_bases
ATGTCCGTAGCCTACGTAGCGCTGCCCGTACCCTTGGCCAGCACCTTTGACTACCTGTTGCCCTCCGACATGCCGGCCCT
GCCGGGCTGCCGGGTCAGCGTGCCCTTTGGCCGCCGTGAGATGATTGGCATTGTGGTGGCGCTTGGCGAGCAGAGTGATC
TGCCGCTGGAAAATCTAAAGCACATTAACCACCTGCTGGATACGCAGCCGCTGTTCAGCGACAGTCAGTGGCGCCTGCTG
CGCTGGGCCGCCGACTATTACCATCACCCCATTGGCGAGGTGCTGTTTCATGCCCTGCCGATGCTGCTGCGCCAGGGCAA
GGCGGCCAGCCATACGCCTTTGTGGCGCTGGCGTATGACGCCTCTGGGGCAGCAAACCGATCCCCTCTCCCTGAAACGGG
CGCCCAAGCAGCAGCAGGCGCTGGCGCTGCTGCAGCGCCACACGATCTACCGCCATCAGACGGAAGAGCTCGATCTCAGC
GCGGCCGCGCTACAGGCACTGAAGAGCAAGGGCCTGATCGCGCTGGAGAGTGAGGCGATTGCCCCCGGTGACTGGCGCAG
CGATGTTTCCCTCTGCACCGATCGCCTGACGCTGAACGCAGAGCAGGCCACTGCCGTGGGCGCCATCCGCGCCGCCGATG
ACCATTTTAGCCCCTGGCTGCTGGCTGGCGTGACCGGCTCAGGCAAGACCGAAGTCTATCTGAGCATCATCGAAAACCTG
CTCGTCCGCGGGCGTCAGGCGCTGGTTCTGGTCCCGGAAATCGGCCTGACGCCGCAGACCATCGCCCGCTTCCGCCAGCG
CTTTTCCGTGCCGGTCGATGTGCTGCACTCCGGGCTGAACGACGGCGAGCGGCTGGCGGCCTGGCTGCGCGCCCGCGACG
GCGAAAGCGCGATCGTTATTGGCACCCGTTCAGCCCTGTTTACGCCGTTCGCCCGCCTGGGCGGCATCATTATCGATGAA
GAGCACGATGGCTCTTACAAACAGCAGGAGGGGTGGCGCTATCACGCCCGCGATCTGGCGGTGCTGCGCGCTCGCCTTGA
GGATATCCCCATCATTCTCGGCTCTGCGACCCCGGCGCTGGAGACCCTGCATAACGTCGACGTCGGCAAGTACCGGCGGC
TGTGTCTGACCCAGCGTGCCGGTAATGCCAAGCCCGCCGCGCAGCATCTGGTGGATCTCAAGGGGCAGCCCCTCAAGGCT
GGACTGGCACCACCGCTGATCGAGCGCATCCGCCGCCACACCGATGCCGGAAACCAGGTGATCCTGTTCCTTAACCGCCG
CGGTTACGCCCCGGCGCTGTTGTGTCATGAGTGCGGCTGGATAGCCGAGTGTCCGCGCTGCGATCACTTCTATACCCTGC
ACCAGGGCCAGCGAACCCTGCGCTGTCACCACTGCGACAGCCAACGGCCGGTTCCACACCAGTGCCCGCAGTGCGGATCT
ACCCATCTGGTGCCGGTCGGGCTGGGGACCGAACAGCTGGAACATGAGCTGACCGATCTGTTTCCCTCGATCCCGCTGAC
CCGCATCGATCGCGATACCACCAGCCGTAAGGGGGCGCTGGAGCAACAGCTGGCAGAGGTCCATCAGGGTGGGGCGCGCA
TCCTGATCGGTACCCAAATGCTGGCCAAGGGGCACCATTTCCCCAATGTCACGCTGGTGGCTTTGCTGGACGTCGACGGT
GCGCTGTTTTCCGCCGATTTTCACGCTGCCGAACGCTTCGCCCAGCTGTATACCCAGGTTGCCGGACGTGCCGGACGAGC
AGGCAAACGCGGCGAAGTATTGCTACAGACCCACCACCCCGACCATCCCCTGCTGCAAACACTACTGCATCAGGGCTATG
ACGCCTTTGCCCGTCAGGCATTGCAGGAGCGTCAGAGTGTATTTCTGCCCCCGTTCACCCACCACGTTATGCTGCGCGCC
GACGATCACGACAACCGACACGCCTGCGATTTTCTGCAGCAGCTGCGCACACGGCTGGAGGCCAGCCCGCTGTGCGACGC
GTCGTTGTGGCTGCTTGGCCCTGTTCCGGCGCTGCAGCCCAAACGCGGCGGCCGTTACCGCTGGCAGCTATTGGCCCAAC
ATCCGTCACGTGTCAAACTGCAGCACCTGTTGCAGGAGGTTTTGCCACAGGTCAGCGCGCTGTCGGCCTATAAGAAGGTG
AAATGGTCGTTAGACGTCGATCCTACCGACAGTTAA

Upstream 100 bases:

>100_bases
GGCGAATCATACAGAAAATAGCCGTCCAGCGCAATCACTCACATCGATCCAGATACTATCCGGTGTACACTGTAACGCCG
TTAAGCCGCAGGAAAAAGCG

Downstream 100 bases:

>100_bases
GGGCGGCGGCGTGGAAATGAAGTGCGATCGGGATCGAAAAAATCAATTCATATCACACTTTCCCTGCAAATTAAGTAACT
GTCACCCCTGATGTTCCGTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MSVAYVALPVPLASTFDYLLPSDMPALPGCRVSVPFGRREMIGIVVALGEQSDLPLENLKHINHLLDTQPLFSDSQWRLL
RWAADYYHHPIGEVLFHALPMLLRQGKAASHTPLWRWRMTPLGQQTDPLSLKRAPKQQQALALLQRHTIYRHQTEELDLS
AAALQALKSKGLIALESEAIAPGDWRSDVSLCTDRLTLNAEQATAVGAIRAADDHFSPWLLAGVTGSGKTEVYLSIIENL
LVRGRQALVLVPEIGLTPQTIARFRQRFSVPVDVLHSGLNDGERLAAWLRARDGESAIVIGTRSALFTPFARLGGIIIDE
EHDGSYKQQEGWRYHARDLAVLRARLEDIPIILGSATPALETLHNVDVGKYRRLCLTQRAGNAKPAAQHLVDLKGQPLKA
GLAPPLIERIRRHTDAGNQVILFLNRRGYAPALLCHECGWIAECPRCDHFYTLHQGQRTLRCHHCDSQRPVPHQCPQCGS
THLVPVGLGTEQLEHELTDLFPSIPLTRIDRDTTSRKGALEQQLAEVHQGGARILIGTQMLAKGHHFPNVTLVALLDVDG
ALFSADFHAAERFAQLYTQVAGRAGRAGKRGEVLLQTHHPDHPLLQTLLHQGYDAFARQALQERQSVFLPPFTHHVMLRA
DDHDNRHACDFLQQLRTRLEASPLCDASLWLLGPVPALQPKRGGRYRWQLLAQHPSRVKLQHLLQEVLPQVSALSAYKKV
KWSLDVDPTDS

Sequences:

>Translated_731_residues
MSVAYVALPVPLASTFDYLLPSDMPALPGCRVSVPFGRREMIGIVVALGEQSDLPLENLKHINHLLDTQPLFSDSQWRLL
RWAADYYHHPIGEVLFHALPMLLRQGKAASHTPLWRWRMTPLGQQTDPLSLKRAPKQQQALALLQRHTIYRHQTEELDLS
AAALQALKSKGLIALESEAIAPGDWRSDVSLCTDRLTLNAEQATAVGAIRAADDHFSPWLLAGVTGSGKTEVYLSIIENL
LVRGRQALVLVPEIGLTPQTIARFRQRFSVPVDVLHSGLNDGERLAAWLRARDGESAIVIGTRSALFTPFARLGGIIIDE
EHDGSYKQQEGWRYHARDLAVLRARLEDIPIILGSATPALETLHNVDVGKYRRLCLTQRAGNAKPAAQHLVDLKGQPLKA
GLAPPLIERIRRHTDAGNQVILFLNRRGYAPALLCHECGWIAECPRCDHFYTLHQGQRTLRCHHCDSQRPVPHQCPQCGS
THLVPVGLGTEQLEHELTDLFPSIPLTRIDRDTTSRKGALEQQLAEVHQGGARILIGTQMLAKGHHFPNVTLVALLDVDG
ALFSADFHAAERFAQLYTQVAGRAGRAGKRGEVLLQTHHPDHPLLQTLLHQGYDAFARQALQERQSVFLPPFTHHVMLRA
DDHDNRHACDFLQQLRTRLEASPLCDASLWLLGPVPALQPKRGGRYRWQLLAQHPSRVKLQHLLQEVLPQVSALSAYKKV
KWSLDVDPTDS
>Mature_730_residues
SVAYVALPVPLASTFDYLLPSDMPALPGCRVSVPFGRREMIGIVVALGEQSDLPLENLKHINHLLDTQPLFSDSQWRLLR
WAADYYHHPIGEVLFHALPMLLRQGKAASHTPLWRWRMTPLGQQTDPLSLKRAPKQQQALALLQRHTIYRHQTEELDLSA
AALQALKSKGLIALESEAIAPGDWRSDVSLCTDRLTLNAEQATAVGAIRAADDHFSPWLLAGVTGSGKTEVYLSIIENLL
VRGRQALVLVPEIGLTPQTIARFRQRFSVPVDVLHSGLNDGERLAAWLRARDGESAIVIGTRSALFTPFARLGGIIIDEE
HDGSYKQQEGWRYHARDLAVLRARLEDIPIILGSATPALETLHNVDVGKYRRLCLTQRAGNAKPAAQHLVDLKGQPLKAG
LAPPLIERIRRHTDAGNQVILFLNRRGYAPALLCHECGWIAECPRCDHFYTLHQGQRTLRCHHCDSQRPVPHQCPQCGST
HLVPVGLGTEQLEHELTDLFPSIPLTRIDRDTTSRKGALEQQLAEVHQGGARILIGTQMLAKGHHFPNVTLVALLDVDGA
LFSADFHAAERFAQLYTQVAGRAGRAGKRGEVLLQTHHPDHPLLQTLLHQGYDAFARQALQERQSVFLPPFTHHVMLRAD
DHDNRHACDFLQQLRTRLEASPLCDASLWLLGPVPALQPKRGGRYRWQLLAQHPSRVKLQHLLQEVLPQVSALSAYKKVK
WSLDVDPTDS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=731, Percent_Identity=71.1354309165527, Blast_Score=1070, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81495; Mature: 81363

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAYVALPVPLASTFDYLLPSDMPALPGCRVSVPFGRREMIGIVVALGEQSDLPLENLK
CCEEEEEECCCHHHHHHHHCCCCCCCCCCCEEECCCCCHHEEEEEEEECCCCCCCHHHHH
HINHLLDTQPLFSDSQWRLLRWAADYYHHPIGEVLFHALPMLLRQGKAASHTPLWRWRMT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
PLGQQTDPLSLKRAPKQQQALALLQRHTIYRHQTEELDLSAAALQALKSKGLIALESEAI
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCEEEECCCC
APGDWRSDVSLCTDRLTLNAEQATAVGAIRAADDHFSPWLLAGVTGSGKTEVYLSIIENL
CCCCCCCHHHHHHHHHEECCHHHHHHHHEEECCCCCCCEEEEECCCCCCHHHHHHHHHHH
LVRGRQALVLVPEIGLTPQTIARFRQRFSVPVDVLHSGLNDGERLAAWLRARDGESAIVI
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEE
GTRSALFTPFARLGGIIIDEEHDGSYKQQEGWRYHARDLAVLRARLEDIPIILGSATPAL
ECCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCHHH
ETLHNVDVGKYRRLCLTQRAGNAKPAAQHLVDLKGQPLKAGLAPPLIERIRRHTDAGNQV
HHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEE
ILFLNRRGYAPALLCHECGWIAECPRCDHFYTLHQGQRTLRCHHCDSQRPVPHQCPQCGS
EEEEECCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCC
THLVPVGLGTEQLEHELTDLFPSIPLTRIDRDTTSRKGALEQQLAEVHQGGARILIGTQM
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHH
LAKGHHFPNVTLVALLDVDGALFSADFHAAERFAQLYTQVAGRAGRAGKRGEVLLQTHHP
HHCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
DHPLLQTLLHQGYDAFARQALQERQSVFLPPFTHHVMLRADDHDNRHACDFLQQLRTRLE
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
ASPLCDASLWLLGPVPALQPKRGGRYRWQLLAQHPSRVKLQHLLQEVLPQVSALSAYKKV
CCCCCCCCCCEECCCCCCCCCCCCCEEEHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHE
KWSLDVDPTDS
EEEECCCCCCC
>Mature Secondary Structure 
SVAYVALPVPLASTFDYLLPSDMPALPGCRVSVPFGRREMIGIVVALGEQSDLPLENLK
CEEEEEECCCHHHHHHHHCCCCCCCCCCCEEECCCCCHHEEEEEEEECCCCCCCHHHHH
HINHLLDTQPLFSDSQWRLLRWAADYYHHPIGEVLFHALPMLLRQGKAASHTPLWRWRMT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
PLGQQTDPLSLKRAPKQQQALALLQRHTIYRHQTEELDLSAAALQALKSKGLIALESEAI
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCEEEECCCC
APGDWRSDVSLCTDRLTLNAEQATAVGAIRAADDHFSPWLLAGVTGSGKTEVYLSIIENL
CCCCCCCHHHHHHHHHEECCHHHHHHHHEEECCCCCCCEEEEECCCCCCHHHHHHHHHHH
LVRGRQALVLVPEIGLTPQTIARFRQRFSVPVDVLHSGLNDGERLAAWLRARDGESAIVI
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEE
GTRSALFTPFARLGGIIIDEEHDGSYKQQEGWRYHARDLAVLRARLEDIPIILGSATPAL
ECCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCHHH
ETLHNVDVGKYRRLCLTQRAGNAKPAAQHLVDLKGQPLKAGLAPPLIERIRRHTDAGNQV
HHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEE
ILFLNRRGYAPALLCHECGWIAECPRCDHFYTLHQGQRTLRCHHCDSQRPVPHQCPQCGS
EEEEECCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCC
THLVPVGLGTEQLEHELTDLFPSIPLTRIDRDTTSRKGALEQQLAEVHQGGARILIGTQM
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHH
LAKGHHFPNVTLVALLDVDGALFSADFHAAERFAQLYTQVAGRAGRAGKRGEVLLQTHHP
HHCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
DHPLLQTLLHQGYDAFARQALQERQSVFLPPFTHHVMLRADDHDNRHACDFLQQLRTRLE
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
ASPLCDASLWLLGPVPALQPKRGGRYRWQLLAQHPSRVKLQHLLQEVLPQVSALSAYKKV
CCCCCCCCCCEECCCCCCCCCCCCCEEEHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHE
KWSLDVDPTDS
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]