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The map label for this gene is hslU

Identifier: 238921643

GI number: 238921643

Start: 3677832

End: 3679163

Strand: Reverse

Name: hslU

Synonym: NT01EI_3798

Alternate gene names: 238921643

Gene position: 3679163-3677832 (Counterclockwise)

Preceding gene: 238921644

Following gene: 238921642

Centisome position: 96.51

GC content: 57.13

Gene sequence:

>1332_bases
ATGTCTGAAATGACCCCACGCGAAATTGTCAGCGAGCTCGACAGTTACATCATTGGCCAACACTCGGCTAAACGCGCCGT
CGCGATCGCGCTGCGTAATCGCTGGCGCCGTATGCAGCTGGGCGAGGCGCTGCGCCACGAAGTCACCCCCAAAAATATTC
TGATGATCGGCCCAACCGGCGTCGGTAAGACCGAAATTGCCCGCCGTCTGGCGAAGCTGGCCAATGCGCCGTTTATCAAG
GTTGAAGCGACCAAGTTCACCGAGGTCGGCTATGTCGGTAAAGAGGTGGACTCCATCATCCGCGATCTTACCGACTCCGC
GATGAAAATGATCCGGGCGCAGTCCATAGAGAAAAACCGCTACCGCGCAGAAGAGATGGCGGAAGAGCGGATTCTGGACG
CCCTGATCCCCCCGGCCAAAAACAACTGGGGGCAGACCGAACAAACCAATGAAAGCTCCCCTGCACGCCAGACCTTTCGC
AAAAAGCTGCGCGAAGGCGAGCTGGATGATAAAGAGATTGAAATCGAGCTGGCGGCCTCCCCGATGGGGGTCGAGATTAT
GGCGCCACCGGGAATGGAGGAGATGACCAGCCAGCTGCAGTCCATGTTCCAAAACCTGGGCGGTCAGAAAACCAAGCCGC
GCAAGGTGAAAATTAAGGATGCTTTCAAACAGCTGATCGAAGAAGAGGCCGCCAAGCTGGTCAATCCGGAAGAGCTGAAA
CAGCAGGCTATCGACGCCGTGGAGCAAAATGGTATTGTCTTCATCGACGAAATCGACAAGATCTGTAAGCGCGGAGAAAG
CTCCGGCCCGGACGTCTCCCGTGAAGGGGTACAGCGCGATCTGTTGCCGCTGATCGAAGGCTGTACCGTCAATACCAAGC
ACGGCATGGTCAAGACCGATCACATTCTATTTATCGCCTCCGGCGCGTTTCAGGTCTCCAGCCCCGCCGATCTGATCCCG
GAGCTGCAGGGCCGTCTGCCTATCCGTGTTGAGCTGCAGGCGCTGACCACCGAAGACTTCGAGCGTATTCTTACGGAGCC
GAGCGCCTCGCTGACCGAGCAGTATCAGGCGCTGATGGCAACCGAAGGCGTTAACATCAGCTTCAGCGCCGACGGTATTC
GCCGCATCGCTGAGGCCGCCTGGCAGGTGAATGAGAGCGCGGAAAACATCGGTGCCCGCCGTCTGCACACGGTGATGGAG
CGTCTGATGGAAGAGATCTCCTTTGACGCCAGCGAAATGCATGGTACGGCGATCGCCATCGATGCCGATTACGTGCGTAA
TCACCTCGATGAGCTGGTAGCAGATGAAGATCTGAGCCGGTTTATTCTATAA

Upstream 100 bases:

>100_bases
CGTAGAAAAGGCGCTGGGAATTGCAGGTGATATCTGCATTTATACCAACCAGTTCCACACCATTGAAGAATTAGACTCCA
AAGCGTAAGGACTGTATATC

Downstream 100 bases:

>100_bases
TCACCCGCAGAAACCGTCCTTTTCGGTCTTTGCTAGGGGGAAGGCGTCTGCCTTCCCCTTTTTTATTGCCACTGGTTGAG
GCAACAGACAGCTAACGGTT

Product: ATP-dependent protease HslVU, ATPase subunit

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSEMTPREIVSELDSYIIGQHSAKRAVAIALRNRWRRMQLGEALRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVDSIIRDLTDSAMKMIRAQSIEKNRYRAEEMAEERILDALIPPAKNNWGQTEQTNESSPARQTFR
KKLREGELDDKEIEIELAASPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKTKPRKVKIKDAFKQLIEEEAAKLVNPEELK
QQAIDAVEQNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGCTVNTKHGMVKTDHILFIASGAFQVSSPADLIP
ELQGRLPIRVELQALTTEDFERILTEPSASLTEQYQALMATEGVNISFSADGIRRIAEAAWQVNESAENIGARRLHTVME
RLMEEISFDASEMHGTAIAIDADYVRNHLDELVADEDLSRFIL

Sequences:

>Translated_443_residues
MSEMTPREIVSELDSYIIGQHSAKRAVAIALRNRWRRMQLGEALRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVDSIIRDLTDSAMKMIRAQSIEKNRYRAEEMAEERILDALIPPAKNNWGQTEQTNESSPARQTFR
KKLREGELDDKEIEIELAASPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKTKPRKVKIKDAFKQLIEEEAAKLVNPEELK
QQAIDAVEQNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGCTVNTKHGMVKTDHILFIASGAFQVSSPADLIP
ELQGRLPIRVELQALTTEDFERILTEPSASLTEQYQALMATEGVNISFSADGIRRIAEAAWQVNESAENIGARRLHTVME
RLMEEISFDASEMHGTAIAIDADYVRNHLDELVADEDLSRFIL
>Mature_442_residues
SEMTPREIVSELDSYIIGQHSAKRAVAIALRNRWRRMQLGEALRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVDSIIRDLTDSAMKMIRAQSIEKNRYRAEEMAEERILDALIPPAKNNWGQTEQTNESSPARQTFRK
KLREGELDDKEIEIELAASPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKTKPRKVKIKDAFKQLIEEEAAKLVNPEELKQ
QAIDAVEQNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGCTVNTKHGMVKTDHILFIASGAFQVSSPADLIPE
LQGRLPIRVELQALTTEDFERILTEPSASLTEQYQALMATEGVNISFSADGIRRIAEAAWQVNESAENIGARRLHTVMER
LMEEISFDASEMHGTAIAIDADYVRNHLDELVADEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=87.1331828442438, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=47, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319704, Length=118, Percent_Identity=33.0508474576271, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648291, Length=249, Percent_Identity=30.1204819277108, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648289, Length=249, Percent_Identity=30.1204819277108, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_EDWI9 (C5BB81)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935158.1
- GeneID:   7960809
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3798
- OMA:   EASKFTE
- ProtClustDB:   CLSK2813736
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49579; Mature: 49448

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 256-256 BINDING 321-321 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVSELDSYIIGQHSAKRAVAIALRNRWRRMQLGEALRHEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDSAMKMIRAQSIEKNR
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAEEMAEERILDALIPPAKNNWGQTEQTNESSPARQTFRKKLREGELDDKEIEIELAAS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
PMGVEIMAPPGMEEMTSQLQSMFQNLGGQKTKPRKVKIKDAFKQLIEEEAAKLVNPEELK
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHH
QQAIDAVEQNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGCTVNTKHGMVKTD
HHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEC
HILFIASGAFQVSSPADLIPELQGRLPIRVELQALTTEDFERILTEPSASLTEQYQALMA
CEEEEECCCEECCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TEGVNISFSADGIRRIAEAAWQVNESAENIGARRLHTVMERLMEEISFDASEMHGTAIAI
HCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEE
DADYVRNHLDELVADEDLSRFIL
EHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEMTPREIVSELDSYIIGQHSAKRAVAIALRNRWRRMQLGEALRHEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDSAMKMIRAQSIEKNR
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAEEMAEERILDALIPPAKNNWGQTEQTNESSPARQTFRKKLREGELDDKEIEIELAAS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
PMGVEIMAPPGMEEMTSQLQSMFQNLGGQKTKPRKVKIKDAFKQLIEEEAAKLVNPEELK
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHH
QQAIDAVEQNGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLIEGCTVNTKHGMVKTD
HHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEC
HILFIASGAFQVSSPADLIPELQGRLPIRVELQALTTEDFERILTEPSASLTEQYQALMA
CEEEEECCCEECCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TEGVNISFSADGIRRIAEAAWQVNESAENIGARRLHTVMERLMEEISFDASEMHGTAIAI
HCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEE
DADYVRNHLDELVADEDLSRFIL
EHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA