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The map label for this gene is uhpA [H]

Identifier: 238921616

GI number: 238921616

Start: 3655715

End: 3656305

Strand: Reverse

Name: uhpA [H]

Synonym: NT01EI_3771

Alternate gene names: 238921616

Gene position: 3656305-3655715 (Counterclockwise)

Preceding gene: 238921617

Following gene: 238921615

Centisome position: 95.91

GC content: 61.59

Gene sequence:

>591_bases
ATGACCTACCGTATTGCCTTTATCGATGACCACTATATCGTGCGATCCGGCTTCGTACAGCTGTTGTCGCTGGAGAAGGA
TATGGCGGTGGTCGGTGAGTTCAGCTCGGCAGCAGAGGCGCGGGCCGGCCTGCCGGGGCTGGATGTGCAGGTCTGTATCT
GTGATATCTCGATGCCTGATGAAAGCGGGTTGGATCTGCTGGCCGATCTGCCCTCCGGGCTGGCGGTGATCATGCTGTCG
ATGCACGACAGCGCGGCGCTGGTAGAGCTGGCGCTGGATCGCGGTGCGCGCGGTTTTCTGTCTAAGCGCTGCCGTCCTGA
GGATCTGGCCAGCGCGGTACGCACTGTCGCACAGGGTGGTGTTTATCTGATGCCGGAGATTGCCCAGCAGCTGGCGCGTA
CCCGCATCGATCCCCTGACCCGCCGCGAGCGTGAGGTGGCGATGATGCTGGCCCAGGGGCTAGAGGTGCGGGAGGTGGCT
GAACGTCTCGGTCTGTCGCCGAAAACCGTACACGTCCATCGTGCCAATCTGTTCATCAAGTTGGGGGTAAACAACAACGT
TGAGCTGGCGCACCGCCTGTTGGGGCAGTGA

Upstream 100 bases:

>100_bases
ATTTCCTGCCTTGATGATGGGTTTCTGGCGCTGCGCCGTTACGGGACCCCTCCGAGTATGTGTATAATCCGAGCGCTATC
GTTATCCGCCGGAGCCCGCC

Downstream 100 bases:

>100_bases
TGCAGCGTCTGATCTCCAGCATTGCCATCTTCTTTTGCTATACCATCGCCGGATTTTGCCTGTGGGCTATTGGTGCGGCA
CTGGTGACGGCGCCCGTCGA

Product: transcriptional regulatory protein UhpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTYRIAFIDDHYIVRSGFVQLLSLEKDMAVVGEFSSAAEARAGLPGLDVQVCICDISMPDESGLDLLADLPSGLAVIMLS
MHDSAALVELALDRGARGFLSKRCRPEDLASAVRTVAQGGVYLMPEIAQQLARTRIDPLTRREREVAMMLAQGLEVREVA
ERLGLSPKTVHVHRANLFIKLGVNNNVELAHRLLGQ

Sequences:

>Translated_196_residues
MTYRIAFIDDHYIVRSGFVQLLSLEKDMAVVGEFSSAAEARAGLPGLDVQVCICDISMPDESGLDLLADLPSGLAVIMLS
MHDSAALVELALDRGARGFLSKRCRPEDLASAVRTVAQGGVYLMPEIAQQLARTRIDPLTRREREVAMMLAQGLEVREVA
ERLGLSPKTVHVHRANLFIKLGVNNNVELAHRLLGQ
>Mature_195_residues
TYRIAFIDDHYIVRSGFVQLLSLEKDMAVVGEFSSAAEARAGLPGLDVQVCICDISMPDESGLDLLADLPSGLAVIMLSM
HDSAALVELALDRGARGFLSKRCRPEDLASAVRTVAQGGVYLMPEIAQQLARTRIDPLTRREREVAMMLAQGLEVREVAE
RLGLSPKTVHVHRANLFIKLGVNNNVELAHRLLGQ

Specific function: Appears to be the positive activator of uhpT transcription [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=190, Percent_Identity=68.9473684210526, Blast_Score=262, Evalue=1e-71,
Organism=Escherichia coli, GI1788222, Length=199, Percent_Identity=35.678391959799, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788521, Length=196, Percent_Identity=35.7142857142857, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788546, Length=200, Percent_Identity=31, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=35.1648351648352, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786747, Length=195, Percent_Identity=27.1794871794872, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788712, Length=183, Percent_Identity=24.5901639344262, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21433; Mature: 21302

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYRIAFIDDHYIVRSGFVQLLSLEKDMAVVGEFSSAAEARAGLPGLDVQVCICDISMPD
CEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHCCCCCCCEEEEEEECCCCC
ESGLDLLADLPSGLAVIMLSMHDSAALVELALDRGARGFLSKRCRPEDLASAVRTVAQGG
CCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCC
VYLMPEIAQQLARTRIDPLTRREREVAMMLAQGLEVREVAERLGLSPKTVHVHRANLFIK
EEECHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEEEEE
LGVNNNVELAHRLLGQ
ECCCCCHHHHHHHHCC
>Mature Secondary Structure 
TYRIAFIDDHYIVRSGFVQLLSLEKDMAVVGEFSSAAEARAGLPGLDVQVCICDISMPD
EEEEEEECCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHCCCCCCCEEEEEEECCCCC
ESGLDLLADLPSGLAVIMLSMHDSAALVELALDRGARGFLSKRCRPEDLASAVRTVAQGG
CCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCC
VYLMPEIAQQLARTRIDPLTRREREVAMMLAQGLEVREVAERLGLSPKTVHVHRANLFIK
EEECHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEEEEE
LGVNNNVELAHRLLGQ
ECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]