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The map label for this gene is ddlA [H]

Identifier: 238921591

GI number: 238921591

Start: 3630794

End: 3631966

Strand: Direct

Name: ddlA [H]

Synonym: NT01EI_3744

Alternate gene names: 238921591

Gene position: 3630794-3631966 (Clockwise)

Preceding gene: 238921588

Following gene: 238921593

Centisome position: 95.24

GC content: 55.67

Gene sequence:

>1173_bases
TTGGCGTTTACCCGGTTCAGCGATATGGTAAAACCTATTTTATTTTTGGTAAGTTATACGGGTTGGATAATGGCAAAAAT
TCGCGTTGGCATTGTGTTCGGCGGTCAATCCTGCGAGCACGAGGTTTCTCTTCAATCAGCAAAAAATATTGTCGAAGCGA
TCGATCAATCACGTTTTGACGTAACACTTATCGGTATCGACAAGCAGGGATACTGGCATCTATGCAGCGGGCAGGACTAC
CTGAACCATGCCCATGACCCACAGCATATCGCCCTGAATCATTCAGAACGCCGTCTGGCGGTGATCCCCGGAGCAATCCA
GGGACAGCTTATCGATAGTGTCAGCGGTGAAATTCTACCCTCGCTGGACGTTATTTTTCCCATCGTACACGGCACGCTGG
GCGAGGATGGCTGCCTGCAGGGTATGCTGAAAATGCTGAACCTGCCCTTTGTCGGATCCGACGTCATCGGCTCCGCTGCC
TGCATGGATAAAGAGGTATGCAAACGCCTGCTGCGCGATGCAGGCTTGCCGATCACCCCCTTCGTCACGCTGACCCGCGC
CACGCAGACCCACTACAACTATGAGACGCTGCGCAGCCAGCTGGGCGAGACGCTGTTTGTCAAGCCGGCCAACCAGGGCT
CCTCCGTCGGCGTCAACAAGGTTCGTAACGCGGATGAGTATCACCGCGCGATTGCCGATGCGCTGCGCTTCGATCATAAG
GTAATCGTCGAACGCGGAGTGATTGGGCATGAGATTGAATGCGCCATCCTCGGTAACGAGGTGGCGCAGGCCAGCGTCTG
TGGCGAGATCGTCCTGCACAGCGACTTTTATTCCTATGACAGCAAGTACATCAGCGCACAAGATGCTCAGGTGGTCGTGC
CGGCAGCGATGGCGCCCGAAGTCAGCGAGCGCGTCCGTCAAACCGCCCTACGGGCGTTTCAGGCGCTGGAGTGCCGCGGT
CTGGCACGCGTCGATGTCTTTGTAACCGCAGAGAATGAAGTCATTATCAATGAGATCAACACACTGCCTGGATTTACCAA
TATCAGTATGTATCCCAAACTGTGGCAGGCCAGCGGTATCAGCTATCAGGCGCTGATCACCCAGCTGATCGAGCTGGCGC
TAGAGCGCCACCGCGTGGACAGCCAGCTGTGCCGGGCGACAGGCAGCCTCTAA

Upstream 100 bases:

>100_bases
TGCGTATCAATCTCAGCGTACACCGCGCCATTCCAGACATATCCTACGGCGATCCCACGGGTAAAATGCGCCATTTTACG
CGGGTCGTAGTGACAAAGGG

Downstream 100 bases:

>100_bases
CGACTGCAGTTCAGAGCCTGCAACGCAGGCTCTTTTTTTACGAAATATCGCGAATAAAGATTAAAAAATCGGTCTCGACT
CCAACTTAGAGCCGCTAACA

Product: hypothetical protein

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAFTRFSDMVKPILFLVSYTGWIMAKIRVGIVFGGQSCEHEVSLQSAKNIVEAIDQSRFDVTLIGIDKQGYWHLCSGQDY
LNHAHDPQHIALNHSERRLAVIPGAIQGQLIDSVSGEILPSLDVIFPIVHGTLGEDGCLQGMLKMLNLPFVGSDVIGSAA
CMDKEVCKRLLRDAGLPITPFVTLTRATQTHYNYETLRSQLGETLFVKPANQGSSVGVNKVRNADEYHRAIADALRFDHK
VIVERGVIGHEIECAILGNEVAQASVCGEIVLHSDFYSYDSKYISAQDAQVVVPAAMAPEVSERVRQTALRAFQALECRG
LARVDVFVTAENEVIINEINTLPGFTNISMYPKLWQASGISYQALITQLIELALERHRVDSQLCRATGSL

Sequences:

>Translated_390_residues
MAFTRFSDMVKPILFLVSYTGWIMAKIRVGIVFGGQSCEHEVSLQSAKNIVEAIDQSRFDVTLIGIDKQGYWHLCSGQDY
LNHAHDPQHIALNHSERRLAVIPGAIQGQLIDSVSGEILPSLDVIFPIVHGTLGEDGCLQGMLKMLNLPFVGSDVIGSAA
CMDKEVCKRLLRDAGLPITPFVTLTRATQTHYNYETLRSQLGETLFVKPANQGSSVGVNKVRNADEYHRAIADALRFDHK
VIVERGVIGHEIECAILGNEVAQASVCGEIVLHSDFYSYDSKYISAQDAQVVVPAAMAPEVSERVRQTALRAFQALECRG
LARVDVFVTAENEVIINEINTLPGFTNISMYPKLWQASGISYQALITQLIELALERHRVDSQLCRATGSL
>Mature_389_residues
AFTRFSDMVKPILFLVSYTGWIMAKIRVGIVFGGQSCEHEVSLQSAKNIVEAIDQSRFDVTLIGIDKQGYWHLCSGQDYL
NHAHDPQHIALNHSERRLAVIPGAIQGQLIDSVSGEILPSLDVIFPIVHGTLGEDGCLQGMLKMLNLPFVGSDVIGSAAC
MDKEVCKRLLRDAGLPITPFVTLTRATQTHYNYETLRSQLGETLFVKPANQGSSVGVNKVRNADEYHRAIADALRFDHKV
IVERGVIGHEIECAILGNEVAQASVCGEIVLHSDFYSYDSKYISAQDAQVVVPAAMAPEVSERVRQTALRAFQALECRGL
ARVDVFVTAENEVIINEINTLPGFTNISMYPKLWQASGISYQALITQLIELALERHRVDSQLCRATGSL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=360, Percent_Identity=66.9444444444444, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1786280, Length=349, Percent_Identity=30.3724928366762, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 42968; Mature: 42837

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTRFSDMVKPILFLVSYTGWIMAKIRVGIVFGGQSCEHEVSLQSAKNIVEAIDQSRFD
CCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCE
VTLIGIDKQGYWHLCSGQDYLNHAHDPQHIALNHSERRLAVIPGAIQGQLIDSVSGEILP
EEEEEECCCCCEEEECCCHHHHCCCCCCEEEECCCCCEEEEECCCHHHHHHHHCCCCCCC
SLDVIFPIVHGTLGEDGCLQGMLKMLNLPFVGSDVIGSAACMDKEVCKRLLRDAGLPITP
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCC
FVTLTRATQTHYNYETLRSQLGETLFVKPANQGSSVGVNKVRNADEYHRAIADALRFDHK
CEEEEEHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCHH
VIVERGVIGHEIECAILGNEVAQASVCGEIVLHSDFYSYDSKYISAQDAQVVVPAAMAPE
HHHHCCCCCCEEEEEEECCHHHHHHHHHHHEEECCHHHCCCCEECCCCCEEEECCCCCHH
VSERVRQTALRAFQALECRGLARVDVFVTAENEVIINEINTLPGFTNISMYPKLWQASGI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEHHCCCCCCCCCCCHHHHHCCCC
SYQALITQLIELALERHRVDSQLCRATGSL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AFTRFSDMVKPILFLVSYTGWIMAKIRVGIVFGGQSCEHEVSLQSAKNIVEAIDQSRFD
CCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCE
VTLIGIDKQGYWHLCSGQDYLNHAHDPQHIALNHSERRLAVIPGAIQGQLIDSVSGEILP
EEEEEECCCCCEEEECCCHHHHCCCCCCEEEECCCCCEEEEECCCHHHHHHHHCCCCCCC
SLDVIFPIVHGTLGEDGCLQGMLKMLNLPFVGSDVIGSAACMDKEVCKRLLRDAGLPITP
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCC
FVTLTRATQTHYNYETLRSQLGETLFVKPANQGSSVGVNKVRNADEYHRAIADALRFDHK
CEEEEEHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCHH
VIVERGVIGHEIECAILGNEVAQASVCGEIVLHSDFYSYDSKYISAQDAQVVVPAAMAPE
HHHHCCCCCCEEEEEEECCHHHHHHHHHHHEEECCHHHCCCCEECCCCCEEEECCCCCHH
VSERVRQTALRAFQALECRGLARVDVFVTAENEVIINEINTLPGFTNISMYPKLWQASGI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEHHCCCCCCCCCCCHHHHHCCCC
SYQALITQLIELALERHRVDSQLCRATGSL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]