Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 238921410

GI number: 238921410

Start: 3462697

End: 3463644

Strand: Direct

Name: fmt

Synonym: NT01EI_3561

Alternate gene names: 238921410

Gene position: 3462697-3463644 (Clockwise)

Preceding gene: 238921409

Following gene: 238921411

Centisome position: 90.83

GC content: 61.6

Gene sequence:

>948_bases
GTGTCTCATTCGCTTCGTATCATTTTTGCGGGTACGCCCGATTTTGCCGCCCGCCATCTTGAGGCGCTCCTGGCCTCGGA
TCATCAGATCGTCGGCGTCTTTACCCAGCCAGATCGGCCGTCAGGACGCGGTAACAAGCTGACGCCGAGTCCGGTCAAGG
CCCTGGCGCTTCAGCATGATCTGCCGGTCTTTCAGCCGGCCTCTCTGCGCCCAGAGGAAAATCAGCGCCTGGTCGCCTCG
CTGCAGGCCGATGTCATGGTCGTGGTCGCCTATGGGCTGATCCTGCCCCAGGCAGTGCTGGATATGCCGCGCCTGGGCTG
TGTTAACGTACACGGCTCTCTGCTGCCGCGCTGGCGCGGCGCGGCGCCAATCCAGCGGGCACTATGGGCGGGCGATAGCG
AAACCGGCGTCACCATTATGCAGATGGACGCCGGGCTGGATACCGGTGATATGCTTCTGAAGCTGTCTTGCCTCATCACC
CAGGACGACACCAGCGCCACACTGTACGATAAGCTGTCCGCGCTGGGTCCCCAAGGGTTGCTAACGACACTGGCTCAACT
CGCCGATGGCCGTGCCCAGGCACAGCAACAGGACGATGCACTCGCCTGCTACGCCGAGAAGCTGAGCAAGGAGGAGGCAC
GCCTAGATTGGCAACTCCCTGCCGAGCAGTTAGCGCGTTGTGTCCGAGCCTTCAACCCTTGGCCTGTCAGCTTCTTCATG
CTGGAAGGACAACCGGTGAAGGTCTGGGGGGCAAACGTCCTTCATCAAGAGAGCGACGCACAGCCAGGCACGATCGTCGG
GGCCGATAAGCAAGGGATCGCCGTCGCGACGGCCCAGGGTATCCTGTTGTTAACTCAGCTGCAGCCTGCCGGTAAGAAAA
GCATGGCCGCCCGTGATCTGCTCAACTCACGCAAAGAGAGTTTCCTCGCGGGAACCCTCCTCGACTAA

Upstream 100 bases:

>100_bases
TATCTGTCACCGCTGAAACGTCAGCGCATCCGTCAAAAGCTGGAAAAGCTGTATAAGCAACAGGCCCGCGCCGTTAACTA
ACCCAAGGCAGGATACTCCC

Downstream 100 bases:

>100_bases
GCATCCTTAGCCCGGTGAACGCCGGGCTACCTCGCTAAAGCGCCCTCCGACGCGGCGTGTCCAATGCGCCATTTCTGCCC
CAATCCGTGATAAACTCCAG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSHSLRIIFAGTPDFAARHLEALLASDHQIVGVFTQPDRPSGRGNKLTPSPVKALALQHDLPVFQPASLRPEENQRLVAS
LQADVMVVVAYGLILPQAVLDMPRLGCVNVHGSLLPRWRGAAPIQRALWAGDSETGVTIMQMDAGLDTGDMLLKLSCLIT
QDDTSATLYDKLSALGPQGLLTTLAQLADGRAQAQQQDDALACYAEKLSKEEARLDWQLPAEQLARCVRAFNPWPVSFFM
LEGQPVKVWGANVLHQESDAQPGTIVGADKQGIAVATAQGILLLTQLQPAGKKSMAARDLLNSRKESFLAGTLLD

Sequences:

>Translated_315_residues
MSHSLRIIFAGTPDFAARHLEALLASDHQIVGVFTQPDRPSGRGNKLTPSPVKALALQHDLPVFQPASLRPEENQRLVAS
LQADVMVVVAYGLILPQAVLDMPRLGCVNVHGSLLPRWRGAAPIQRALWAGDSETGVTIMQMDAGLDTGDMLLKLSCLIT
QDDTSATLYDKLSALGPQGLLTTLAQLADGRAQAQQQDDALACYAEKLSKEEARLDWQLPAEQLARCVRAFNPWPVSFFM
LEGQPVKVWGANVLHQESDAQPGTIVGADKQGIAVATAQGILLLTQLQPAGKKSMAARDLLNSRKESFLAGTLLD
>Mature_314_residues
SHSLRIIFAGTPDFAARHLEALLASDHQIVGVFTQPDRPSGRGNKLTPSPVKALALQHDLPVFQPASLRPEENQRLVASL
QADVMVVVAYGLILPQAVLDMPRLGCVNVHGSLLPRWRGAAPIQRALWAGDSETGVTIMQMDAGLDTGDMLLKLSCLITQ
DDTSATLYDKLSALGPQGLLTTLAQLADGRAQAQQQDDALACYAEKLSKEEARLDWQLPAEQLARCVRAFNPWPVSFFML
EGQPVKVWGANVLHQESDAQPGTIVGADKQGIAVATAQGILLLTQLQPAGKKSMAARDLLNSRKESFLAGTLLD

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=294, Percent_Identity=30.6122448979592, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI21614513, Length=290, Percent_Identity=31.3793103448276, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=34.0425531914894, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=74.5222929936306, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1788589, Length=262, Percent_Identity=32.4427480916031, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI133930964, Length=263, Percent_Identity=28.8973384030418, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319458, Length=158, Percent_Identity=32.9113924050633, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=29.8360655737705, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=31.858407079646, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585660, Length=246, Percent_Identity=33.739837398374, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_EDWI9 (C5BF18)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934925.1
- GeneID:   7961383
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3561
- OMA:   IMQMDEG
- ProtClustDB:   CLSK2813667
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33939; Mature: 33808

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSLRIIFAGTPDFAARHLEALLASDHQIVGVFTQPDRPSGRGNKLTPSPVKALALQHD
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
LPVFQPASLRPEENQRLVASLQADVMVVVAYGLILPQAVLDMPRLGCVNVHGSLLPRWRG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
AAPIQRALWAGDSETGVTIMQMDAGLDTGDMLLKLSCLITQDDTSATLYDKLSALGPQGL
CCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHCCCHHH
LTTLAQLADGRAQAQQQDDALACYAEKLSKEEARLDWQLPAEQLARCVRAFNPWPVSFFM
HHHHHHHHCCHHHHHHCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEE
LEGQPVKVWGANVLHQESDAQPGTIVGADKQGIAVATAQGILLLTQLQPAGKKSMAARDL
ECCCEEEEECCHHHCCCCCCCCCEEEECCCCCEEEEECCCEEEEEECCCCCHHHHHHHHH
LNSRKESFLAGTLLD
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHSLRIIFAGTPDFAARHLEALLASDHQIVGVFTQPDRPSGRGNKLTPSPVKALALQHD
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
LPVFQPASLRPEENQRLVASLQADVMVVVAYGLILPQAVLDMPRLGCVNVHGSLLPRWRG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
AAPIQRALWAGDSETGVTIMQMDAGLDTGDMLLKLSCLITQDDTSATLYDKLSALGPQGL
CCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHCCCHHH
LTTLAQLADGRAQAQQQDDALACYAEKLSKEEARLDWQLPAEQLARCVRAFNPWPVSFFM
HHHHHHHHCCHHHHHHCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEE
LEGQPVKVWGANVLHQESDAQPGTIVGADKQGIAVATAQGILLLTQLQPAGKKSMAARDL
ECCCEEEEECCHHHCCCCCCCCCEEEECCCCCEEEEECCCEEEEEECCCCCHHHHHHHHH
LNSRKESFLAGTLLD
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA