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The map label for this gene is def

Identifier: 238921409

GI number: 238921409

Start: 3462162

End: 3462677

Strand: Direct

Name: def

Synonym: NT01EI_3560

Alternate gene names: 238921409

Gene position: 3462162-3462677 (Clockwise)

Preceding gene: 238921402

Following gene: 238921410

Centisome position: 90.82

GC content: 54.26

Gene sequence:

>516_bases
ATGGCGGTATTGCAGGTATTACATTTCCCCGATGAGCGGCTTCGCACTATTGCTAAGCCGGTACACGAAGTTACGCCCGA
AATCCAGCGTATTGTAGACGATATGTTCGAGACCATGTACGCGGAAGAGGGCATTGGCCTCGCCGCGACGCAGGTGGACA
TTCATCAGCGTATTATCGTCATTGACGTGTCAGAGAATCGCGATCAGCGCCTGGTATTGATCAACCCGGAGCTGCTGCAG
CATGACGGGGAGGCCGGTATCGAAGAAGGATGTCTCTCCGTTCCTGAACAGCGGGCGCTGGTGACCCGCGCTGAAAACAT
CAAAGTGCGCGCCTTGGATCGCGACGGCAAGTCCTTTGAGCTGGAGACCGACGGTCTGCTGGCAATCTGCATTCAGCACG
AGATGGATCACCTGATGGGTAAGCTGTTTATTGACTATCTGTCACCGCTGAAACGTCAGCGCATCCGTCAAAAGCTGGAA
AAGCTGTATAAGCAACAGGCCCGCGCCGTTAACTAA

Upstream 100 bases:

>100_bases
GATGTGTTCAATAGGTGAACAATACTGTCAATCAGGGGGAGAACTGTCTAGAATAGGAGTGAAAATCATTCCCTAGCTCA
GAGGACGTTTAAAGAGATTT

Downstream 100 bases:

>100_bases
CCCAAGGCAGGATACTCCCGTGTCTCATTCGCTTCGTATCATTTTTGCGGGTACGCCCGATTTTGCCGCCCGCCATCTTG
AGGCGCTCCTGGCCTCGGAT

Product: peptide deformylase (PDF)

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAVLQVLHFPDERLRTIAKPVHEVTPEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDQRLVLINPELLQ
HDGEAGIEEGCLSVPEQRALVTRAENIKVRALDRDGKSFELETDGLLAICIQHEMDHLMGKLFIDYLSPLKRQRIRQKLE
KLYKQQARAVN

Sequences:

>Translated_171_residues
MAVLQVLHFPDERLRTIAKPVHEVTPEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDQRLVLINPELLQ
HDGEAGIEEGCLSVPEQRALVTRAENIKVRALDRDGKSFELETDGLLAICIQHEMDHLMGKLFIDYLSPLKRQRIRQKLE
KLYKQQARAVN
>Mature_170_residues
AVLQVLHFPDERLRTIAKPVHEVTPEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDQRLVLINPELLQH
DGEAGIEEGCLSVPEQRALVTRAENIKVRALDRDGKSFELETDGLLAICIQHEMDHLMGKLFIDYLSPLKRQRIRQKLEK
LYKQQARAVN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=31.2101910828025, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=84.0236686390533, Blast_Score=292, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24645726, Length=143, Percent_Identity=32.8671328671329, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24645728, Length=136, Percent_Identity=32.3529411764706, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_EDWI9 (C5BF17)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934924.1
- GeneID:   7961382
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3560
- OMA:   MVIKKFT
- ProtClustDB:   CLSK2813666
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19660; Mature: 19529

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLQVLHFPDERLRTIAKPVHEVTPEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIV
CCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCEEEE
IDVSENRDQRLVLINPELLQHDGEAGIEEGCLSVPEQRALVTRAENIKVRALDRDGKSFE
EEECCCCCCEEEEECHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCEEE
LETDGLLAICIQHEMDHLMGKLFIDYLSPLKRQRIRQKLEKLYKQQARAVN
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVLQVLHFPDERLRTIAKPVHEVTPEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIV
CHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCEEEE
IDVSENRDQRLVLINPELLQHDGEAGIEEGCLSVPEQRALVTRAENIKVRALDRDGKSFE
EEECCCCCCEEEEECHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCEEE
LETDGLLAICIQHEMDHLMGKLFIDYLSPLKRQRIRQKLEKLYKQQARAVN
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA