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The map label for this gene is potE [H]

Identifier: 238921269

GI number: 238921269

Start: 3299590

End: 3300897

Strand: Direct

Name: potE [H]

Synonym: NT01EI_3412

Alternate gene names: 238921269

Gene position: 3299590-3300897 (Clockwise)

Preceding gene: 238921268

Following gene: 238921270

Centisome position: 86.55

GC content: 58.41

Gene sequence:

>1308_bases
ATGGGCGTCGTGCAGTTAACCATACTGACGGCGGTGAACATGATGGGGTCCGGCATCATTATGCTGCCGACCAAGCTGGC
CTCCGTGGGGACCATGTCGATACTCTCCTGGCTGGTCACCGCCTTCGGCAGCCTGTGTCTGGCCTACGGTTTTTCCAAGT
GCGGGATGTTCAGCCGCAAGTCCGGCGGGATGGGCGGCTACGCGGAGTACGCCTTCGGCAAGTCCGGTAACTTTATGGCC
AACTTCACCTACGCCATCAGCCTGGTGATCGCCAATATCGCCATCGCCATCTCCGCCGTCGGCTACGGCGCCGTGCTGTT
CGACTGGCACCCATCGCCGATCATGACCTGTATCGCCACCATCGCCGTCATCTGGCTCACCACCGTAGCCAATTTTGGCG
GGGCACGCATCACCGGCAGCATCGGCTCCATCACCGTCTGGGGAGTGATCATTCCGGTGGTCGGCATCTCCATTCTCGGC
TGGTTCTGGTTTAGCCCGAATATGTACATCCAGGCCTGGAACCCGCATCATCTCTCCTTCTCATCCGGTCTGGGCGCCTC
CATCGCCATGACCCTGTGGGCCTTCCTGGGGCTGGAGTCTGCCTGCGCCAACTCCGATGCCGTGGAAAACCCGGAAAAAA
ATATCCCGGTCGCCGTCTTCGGCGGCACCCTGATCTGCGCCATCGTGTATATCCTGTCCACCAACGTGATGGCCGGCTTT
GTGCCCAACATGGATCTGGCCAATTCGACCGCGCCGTTCGGCCTGGCCTTCTCCTATATGTTCGACCCAACGGTCGGTAA
GATCATCATGGCCCTGATGGTCGTCGCCTGTATCGGCTCCCTGCTGGGCTGGCAATTCACCGTAGCCCAGGTGTTTAAGT
CCTCTTCCGATATCGGCTACTTCCCGCAGATCTTCGCCAAGGTCACCGCCAAGGATGCACCGATCGCCGGGATGATCATT
CTGGGGATAGCGCAAACCATCCTGTCGCTGATGACCATCAGCCCATCGCTGAACAATCAGTTCAACTCCCTGGTTAACCT
GGCGGTGGTCACCAACCTGATCCCCTACATCCTCTCCATGGCAGCGCTGATCCCGATGCACCGCCTGTACAAGATGGCCA
ACAAGACCCATGTGGTAATCCTACTCACCGCCATCATCGGTACTGTTTACAGCTTCTACGCCCTCTACTCATCCGGCTCA
GAGGCCATGCTGATGGGGTCACTGGTGACCTTTGCCGGCTGGAGTTGCTATGGCCTGTTCGTGCGGGACAACAACACCTG
CCCGAAAGACAAAATTCAACATGTTTAA

Upstream 100 bases:

>100_bases
TAAAACAAGGTTGGCGGGGGGCCGCGGCTCCTGGGTTGCTCCTGCCCGGATCCCCGCATTTTGAGAGAGAGAAACTATGA
GTACGTCTAGTGGCAATAAG

Downstream 100 bases:

>100_bases
CCCGGAGAACGTGAAATGAAAGACATTCTGTTCCTGTGTAACCCCTCGCCGACCTTCAAGCGCATCCCGCTGGAGGAGCT
GTACGCCCAGCTGCGCAATC

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MGVVQLTILTAVNMMGSGIIMLPTKLASVGTMSILSWLVTAFGSLCLAYGFSKCGMFSRKSGGMGGYAEYAFGKSGNFMA
NFTYAISLVIANIAIAISAVGYGAVLFDWHPSPIMTCIATIAVIWLTTVANFGGARITGSIGSITVWGVIIPVVGISILG
WFWFSPNMYIQAWNPHHLSFSSGLGASIAMTLWAFLGLESACANSDAVENPEKNIPVAVFGGTLICAIVYILSTNVMAGF
VPNMDLANSTAPFGLAFSYMFDPTVGKIIMALMVVACIGSLLGWQFTVAQVFKSSSDIGYFPQIFAKVTAKDAPIAGMII
LGIAQTILSLMTISPSLNNQFNSLVNLAVVTNLIPYILSMAALIPMHRLYKMANKTHVVILLTAIIGTVYSFYALYSSGS
EAMLMGSLVTFAGWSCYGLFVRDNNTCPKDKIQHV

Sequences:

>Translated_435_residues
MGVVQLTILTAVNMMGSGIIMLPTKLASVGTMSILSWLVTAFGSLCLAYGFSKCGMFSRKSGGMGGYAEYAFGKSGNFMA
NFTYAISLVIANIAIAISAVGYGAVLFDWHPSPIMTCIATIAVIWLTTVANFGGARITGSIGSITVWGVIIPVVGISILG
WFWFSPNMYIQAWNPHHLSFSSGLGASIAMTLWAFLGLESACANSDAVENPEKNIPVAVFGGTLICAIVYILSTNVMAGF
VPNMDLANSTAPFGLAFSYMFDPTVGKIIMALMVVACIGSLLGWQFTVAQVFKSSSDIGYFPQIFAKVTAKDAPIAGMII
LGIAQTILSLMTISPSLNNQFNSLVNLAVVTNLIPYILSMAALIPMHRLYKMANKTHVVILLTAIIGTVYSFYALYSSGS
EAMLMGSLVTFAGWSCYGLFVRDNNTCPKDKIQHV
>Mature_434_residues
GVVQLTILTAVNMMGSGIIMLPTKLASVGTMSILSWLVTAFGSLCLAYGFSKCGMFSRKSGGMGGYAEYAFGKSGNFMAN
FTYAISLVIANIAIAISAVGYGAVLFDWHPSPIMTCIATIAVIWLTTVANFGGARITGSIGSITVWGVIIPVVGISILGW
FWFSPNMYIQAWNPHHLSFSSGLGASIAMTLWAFLGLESACANSDAVENPEKNIPVAVFGGTLICAIVYILSTNVMAGFV
PNMDLANSTAPFGLAFSYMFDPTVGKIIMALMVVACIGSLLGWQFTVAQVFKSSSDIGYFPQIFAKVTAKDAPIAGMIIL
GIAQTILSLMTISPSLNNQFNSLVNLAVVTNLIPYILSMAALIPMHRLYKMANKTHVVILLTAIIGTVYSFYALYSSGSE
AMLMGSLVTFAGWSCYGLFVRDNNTCPKDKIQHV

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=419, Percent_Identity=71.8377088305489, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI2367353, Length=412, Percent_Identity=34.4660194174757, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1790575, Length=408, Percent_Identity=31.3725490196078, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1787890, Length=450, Percent_Identity=25.1111111111111, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32566699, Length=303, Percent_Identity=27.0627062706271, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46342; Mature: 46211

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVQLTILTAVNMMGSGIIMLPTKLASVGTMSILSWLVTAFGSLCLAYGFSKCGMFSRK
CCEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGGMGGYAEYAFGKSGNFMANFTYAISLVIANIAIAISAVGYGAVLFDWHPSPIMTCIAT
CCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
IAVIWLTTVANFGGARITGSIGSITVWGVIIPVVGISILGWFWFSPNMYIQAWNPHHLSF
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECC
SSGLGASIAMTLWAFLGLESACANSDAVENPEKNIPVAVFGGTLICAIVYILSTNVMAGF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCC
VPNMDLANSTAPFGLAFSYMFDPTVGKIIMALMVVACIGSLLGWQFTVAQVFKSSSDIGY
CCCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FPQIFAKVTAKDAPIAGMIILGIAQTILSLMTISPSLNNQFNSLVNLAVVTNLIPYILSM
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AALIPMHRLYKMANKTHVVILLTAIIGTVYSFYALYSSGSEAMLMGSLVTFAGWSCYGLF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEE
VRDNNTCPKDKIQHV
EECCCCCCHHHCCCC
>Mature Secondary Structure 
GVVQLTILTAVNMMGSGIIMLPTKLASVGTMSILSWLVTAFGSLCLAYGFSKCGMFSRK
CEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGGMGGYAEYAFGKSGNFMANFTYAISLVIANIAIAISAVGYGAVLFDWHPSPIMTCIAT
CCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
IAVIWLTTVANFGGARITGSIGSITVWGVIIPVVGISILGWFWFSPNMYIQAWNPHHLSF
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECC
SSGLGASIAMTLWAFLGLESACANSDAVENPEKNIPVAVFGGTLICAIVYILSTNVMAGF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCC
VPNMDLANSTAPFGLAFSYMFDPTVGKIIMALMVVACIGSLLGWQFTVAQVFKSSSDIGY
CCCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FPQIFAKVTAKDAPIAGMIILGIAQTILSLMTISPSLNNQFNSLVNLAVVTNLIPYILSM
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AALIPMHRLYKMANKTHVVILLTAIIGTVYSFYALYSSGSEAMLMGSLVTFAGWSCYGLF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEE
VRDNNTCPKDKIQHV
EECCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]