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The map label for this gene is fhlA [H]

Identifier: 238921147

GI number: 238921147

Start: 3166734

End: 3168866

Strand: Reverse

Name: fhlA [H]

Synonym: NT01EI_3288

Alternate gene names: 238921147

Gene position: 3168866-3166734 (Counterclockwise)

Preceding gene: 238921148

Following gene: 238921146

Centisome position: 83.12

GC content: 61.37

Gene sequence:

>2133_bases
ATGAATAGCACGAAAACCTGTTCGTTATCGCGCCTGGAGCCGATCTGGCAGGATTTGTTGCATCAGGCGACGCAGAGTCT
GCTGGCTCAGCGTTCGCTTCCCGAGCTGATGCGCGCGCTGCATCAGCTCTCCTTCTCCGCGATCCGTTTCGATCGCGTCA
ATATTCTGCGCTTGGATCCGCTGCATAACCGCATCACTCTGTATCGCTACGATCCACAGCAAGATCGGGTGATCCAGGAC
GGCGATGTCCTGCTGGCCGATGGCCCCGGTGGGCGGGTCTGGACCGAACAGCGCCCGGTCTGCTGCTCGGGCGCTGATTT
CTGCCTTGACTTCGCCGCCGTCGCCGCGCTGCCGCACTATGCCGGGATTACGGCCTATTGCCAGCTGCCGCTGACCACGG
ATCGGCAGCGTCTGGGGGGTATCGAATTCATTAAGTATCGGGCCGATGCCTTCAGCGAGGGGGAGCTGGCGCTGGCGGCA
CCGCTGGCTGAGATTGTGGCGCAGATCTTCGAGCGTCTGCTGGAGCGGGAGCAGTTCCAGCAACAGGAGGAGCTGCTGCG
TCGCGAGCGCGACCACTTTCGTATTTTGGTGGACGTGACCAATGCGGTGACCTCCAAGCTGGAGATGGATGCGCTGGCGG
GCGAGGTCTCCAAAGAGATCCACCGCTTCTTCGGCATCGACTACATCGGACTGTCGCTGATTGAGCAGGGCGACGAGCGG
CTTAAGTCCTATATTACCGGCTATCAGAGCGGTAAGGCGGTGCTGCGCGAGCAAAAGCTGCTGCCGCTGGCTGGCTCACT
GGCTCAGCGGGTGATCCGGGATAAGACCCTGCTGCTGATGGATCTCTCCGATTCTGCCCGCCTGGCACCGCAGGATCGTC
AACTGGCTCAGATGCTGAGTTTTGGCAACCGTACCGTCTGTCAGTTGCCGCTGATCTTTGGCAATAAGGTGCTGGGCGTG
CTGAAGCTGGCCCAATGCCAGGCGGGAGTATTTAATCCGCGTAACCTGAAACTGTTGCGCCAGATCGCCGCCCGCATCGC
CATTGCCGTCGATAATGCGCTGGCCTACGGCGAGATTTCGCGTCTGAAGGACAGCCTGGTACATGAGAATCGCTATCTGA
CGGAGTGCATCCGCCAAGAGGGTGACTTTGGTGAGATTGTCGGTCAGAGCGAGGCGATGCGCAGCGTGTTGGAGCAGGTG
GAGATGGTGGCCGCCAGCGACTGCAGCGTGCTGATCCTGGGGGAGACCGGTACCGGTAAGGAGTTGATCGCACAGGCGAT
CCATAATCTCAGCCTGCGTAATCAGAAGCGGATGGTCAAGATGAACTGCGCCGCTATTCCCAGCGGCCTGCTGGAGAGCG
ATCTGTTCGGTCACGAGAAGGGGGCTTTTACCGGCGCCAGCGCCCTGCGCATGGGGCGCTTTGAGCTGGCCAACCACGGT
ACACTGTTTCTGGACGAGGTCGGGGATATCCCGCTGGAGCTGCAGCCCAAGCTGTTGCGGGTGCTGCAGGAGCGCGAGAT
CGAGCGTCTTGGCGGCAGTCGGGTGATCCCGGTGGATGTGCGGCTGATCGCCGCCACTAACCGCGATCTGAAGCAGATGG
TGCAGGATCGCCAGTATCGCAGCGATCTCTACTACCGGCTCAACGTTTTCCCTATCGTTATTCCGCCACTGCGGGAGCGT
CCCGGGGATATCCCGCTGCTGGTTAAGTTCTTCACGCGTAAGATAGCCAAGCGGATGAACCGCACTATCGACAGCATTCC
AACGGAGACCATTCGTATGCTGAGCCAGCTGCCCTGGCCGGGCAACGTGCGTGAGCTGGAGAATATTATCGAGCGGGCGG
TGATCCTGAGCCGTGGTCCGGTGCTGAATCTGCCGCTGGATGAGCTGCAGTATCACCTTTCTCCGCCGCTGGCCAGCCGC
AGTATGCCGATGCGGGAGCCGATGGTGCCCGCATCGGCAGCGTCGGAGGAGAGAGCGCAGAGCGAGCGGGATCGTATCCT
GCAAGTGCTGCGCGAATGCAACGGCATCGTCGCCGGGCCGCGCGGCGCGGCGGTTCGTCTGGGGCTGAAGCGTACGACGC
TGATTTCGCGCATGCAGCGTCTGGGGATCTCGGCCCACGACGCCACCCAGTAG

Upstream 100 bases:

>100_bases
CAGTTGGACCGGAGCCATAGGCTGCCGCCCGCCTCTTGCCTCCCATTTTTGGCTCAGCGCCGACGCCACGCACCGTTACA
GCAATGGGATACAGTCGAGT

Downstream 100 bases:

>100_bases
CTTCAGGTTTCGCCAGCGGTGTCATGGCGGCATCGGCACGAGTGTCGGTGCCGCCGTCTTTTTGGGACGTACAGCGTGCC
ATGCGGCATCTTCTCTGGCG

Product: formate hydrogenlyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MNSTKTCSLSRLEPIWQDLLHQATQSLLAQRSLPELMRALHQLSFSAIRFDRVNILRLDPLHNRITLYRYDPQQDRVIQD
GDVLLADGPGGRVWTEQRPVCCSGADFCLDFAAVAALPHYAGITAYCQLPLTTDRQRLGGIEFIKYRADAFSEGELALAA
PLAEIVAQIFERLLEREQFQQQEELLRRERDHFRILVDVTNAVTSKLEMDALAGEVSKEIHRFFGIDYIGLSLIEQGDER
LKSYITGYQSGKAVLREQKLLPLAGSLAQRVIRDKTLLLMDLSDSARLAPQDRQLAQMLSFGNRTVCQLPLIFGNKVLGV
LKLAQCQAGVFNPRNLKLLRQIAARIAIAVDNALAYGEISRLKDSLVHENRYLTECIRQEGDFGEIVGQSEAMRSVLEQV
EMVAASDCSVLILGETGTGKELIAQAIHNLSLRNQKRMVKMNCAAIPSGLLESDLFGHEKGAFTGASALRMGRFELANHG
TLFLDEVGDIPLELQPKLLRVLQEREIERLGGSRVIPVDVRLIAATNRDLKQMVQDRQYRSDLYYRLNVFPIVIPPLRER
PGDIPLLVKFFTRKIAKRMNRTIDSIPTETIRMLSQLPWPGNVRELENIIERAVILSRGPVLNLPLDELQYHLSPPLASR
SMPMREPMVPASAASEERAQSERDRILQVLRECNGIVAGPRGAAVRLGLKRTTLISRMQRLGISAHDATQ

Sequences:

>Translated_710_residues
MNSTKTCSLSRLEPIWQDLLHQATQSLLAQRSLPELMRALHQLSFSAIRFDRVNILRLDPLHNRITLYRYDPQQDRVIQD
GDVLLADGPGGRVWTEQRPVCCSGADFCLDFAAVAALPHYAGITAYCQLPLTTDRQRLGGIEFIKYRADAFSEGELALAA
PLAEIVAQIFERLLEREQFQQQEELLRRERDHFRILVDVTNAVTSKLEMDALAGEVSKEIHRFFGIDYIGLSLIEQGDER
LKSYITGYQSGKAVLREQKLLPLAGSLAQRVIRDKTLLLMDLSDSARLAPQDRQLAQMLSFGNRTVCQLPLIFGNKVLGV
LKLAQCQAGVFNPRNLKLLRQIAARIAIAVDNALAYGEISRLKDSLVHENRYLTECIRQEGDFGEIVGQSEAMRSVLEQV
EMVAASDCSVLILGETGTGKELIAQAIHNLSLRNQKRMVKMNCAAIPSGLLESDLFGHEKGAFTGASALRMGRFELANHG
TLFLDEVGDIPLELQPKLLRVLQEREIERLGGSRVIPVDVRLIAATNRDLKQMVQDRQYRSDLYYRLNVFPIVIPPLRER
PGDIPLLVKFFTRKIAKRMNRTIDSIPTETIRMLSQLPWPGNVRELENIIERAVILSRGPVLNLPLDELQYHLSPPLASR
SMPMREPMVPASAASEERAQSERDRILQVLRECNGIVAGPRGAAVRLGLKRTTLISRMQRLGISAHDATQ
>Mature_710_residues
MNSTKTCSLSRLEPIWQDLLHQATQSLLAQRSLPELMRALHQLSFSAIRFDRVNILRLDPLHNRITLYRYDPQQDRVIQD
GDVLLADGPGGRVWTEQRPVCCSGADFCLDFAAVAALPHYAGITAYCQLPLTTDRQRLGGIEFIKYRADAFSEGELALAA
PLAEIVAQIFERLLEREQFQQQEELLRRERDHFRILVDVTNAVTSKLEMDALAGEVSKEIHRFFGIDYIGLSLIEQGDER
LKSYITGYQSGKAVLREQKLLPLAGSLAQRVIRDKTLLLMDLSDSARLAPQDRQLAQMLSFGNRTVCQLPLIFGNKVLGV
LKLAQCQAGVFNPRNLKLLRQIAARIAIAVDNALAYGEISRLKDSLVHENRYLTECIRQEGDFGEIVGQSEAMRSVLEQV
EMVAASDCSVLILGETGTGKELIAQAIHNLSLRNQKRMVKMNCAAIPSGLLESDLFGHEKGAFTGASALRMGRFELANHG
TLFLDEVGDIPLELQPKLLRVLQEREIERLGGSRVIPVDVRLIAATNRDLKQMVQDRQYRSDLYYRLNVFPIVIPPLRER
PGDIPLLVKFFTRKIAKRMNRTIDSIPTETIRMLSQLPWPGNVRELENIIERAVILSRGPVLNLPLDELQYHLSPPLASR
SMPMREPMVPASAASEERAQSERDRILQVLRECNGIVAGPRGAAVRLGLKRTTLISRMQRLGISAHDATQ

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=700, Percent_Identity=52.8571428571429, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=600, Percent_Identity=50.6666666666667, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1788550, Length=343, Percent_Identity=45.1895043731778, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=45.2830188679245, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1788905, Length=323, Percent_Identity=44.5820433436532, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=50.6382978723404, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1790299, Length=342, Percent_Identity=40.6432748538012, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI1789233, Length=244, Percent_Identity=48.3606557377049, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI87081872, Length=245, Percent_Identity=46.530612244898, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI1786524, Length=377, Percent_Identity=38.4615384615385, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787583, Length=322, Percent_Identity=36.0248447204969, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081858, Length=302, Percent_Identity=33.7748344370861, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789828, Length=245, Percent_Identity=35.9183673469388, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 79749; Mature: 79749

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTKTCSLSRLEPIWQDLLHQATQSLLAQRSLPELMRALHQLSFSAIRFDRVNILRLDP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEECC
LHNRITLYRYDPQQDRVIQDGDVLLADGPGGRVWTEQRPVCCSGADFCLDFAAVAALPHY
CCCCEEEEEECCCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGITAYCQLPLTTDRQRLGGIEFIKYRADAFSEGELALAAPLAEIVAQIFERLLEREQFQ
CCEEEEEECCCCCCHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QQEELLRRERDHFRILVDVTNAVTSKLEMDALAGEVSKEIHRFFGIDYIGLSLIEQGDER
HHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LKSYITGYQSGKAVLREQKLLPLAGSLAQRVIRDKTLLLMDLSDSARLAPQDRQLAQMLS
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHH
FGNRTVCQLPLIFGNKVLGVLKLAQCQAGVFNPRNLKLLRQIAARIAIAVDNALAYGEIS
CCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHH
RLKDSLVHENRYLTECIRQEGDFGEIVGQSEAMRSVLEQVEMVAASDCSVLILGETGTGK
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
ELIAQAIHNLSLRNQKRMVKMNCAAIPSGLLESDLFGHEKGAFTGASALRMGRFELANHG
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCHHHCCCC
TLFLDEVGDIPLELQPKLLRVLQEREIERLGGSRVIPVDVRLIAATNRDLKQMVQDRQYR
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHH
SDLYYRLNVFPIVIPPLRERPGDIPLLVKFFTRKIAKRMNRTIDSIPTETIRMLSQLPWP
HHHEEEEEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
GNVRELENIIERAVILSRGPVLNLPLDELQYHLSPPLASRSMPMREPMVPASAASEERAQ
CCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SERDRILQVLRECNGIVAGPRGAAVRLGLKRTTLISRMQRLGISAHDATQ
HHHHHHHHHHHHCCCEEECCCCCEEEECHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNSTKTCSLSRLEPIWQDLLHQATQSLLAQRSLPELMRALHQLSFSAIRFDRVNILRLDP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEECC
LHNRITLYRYDPQQDRVIQDGDVLLADGPGGRVWTEQRPVCCSGADFCLDFAAVAALPHY
CCCCEEEEEECCCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGITAYCQLPLTTDRQRLGGIEFIKYRADAFSEGELALAAPLAEIVAQIFERLLEREQFQ
CCEEEEEECCCCCCHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QQEELLRRERDHFRILVDVTNAVTSKLEMDALAGEVSKEIHRFFGIDYIGLSLIEQGDER
HHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LKSYITGYQSGKAVLREQKLLPLAGSLAQRVIRDKTLLLMDLSDSARLAPQDRQLAQMLS
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHH
FGNRTVCQLPLIFGNKVLGVLKLAQCQAGVFNPRNLKLLRQIAARIAIAVDNALAYGEIS
CCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHH
RLKDSLVHENRYLTECIRQEGDFGEIVGQSEAMRSVLEQVEMVAASDCSVLILGETGTGK
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
ELIAQAIHNLSLRNQKRMVKMNCAAIPSGLLESDLFGHEKGAFTGASALRMGRFELANHG
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCHHHCCCC
TLFLDEVGDIPLELQPKLLRVLQEREIERLGGSRVIPVDVRLIAATNRDLKQMVQDRQYR
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHH
SDLYYRLNVFPIVIPPLRERPGDIPLLVKFFTRKIAKRMNRTIDSIPTETIRMLSQLPWP
HHHEEEEEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
GNVRELENIIERAVILSRGPVLNLPLDELQYHLSPPLASRSMPMREPMVPASAASEERAQ
CCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SERDRILQVLRECNGIVAGPRGAAVRLGLKRTTLISRMQRLGISAHDATQ
HHHHHHHHHHHHCCCEEECCCCCEEEECHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]